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$##+**111888>>>DDGJJPQPVVV\\\bbbggglllrrqvvv|{{~~~~~~~~~~~~~~~}}}}}}}}}~}~}~}~}~~}}}}~~~}}~~}}}}~~~}}}~}~~}~~}~~~~~~~~~~~~~~~~~~~~}~}}~~~~}~~~~~}}~~}~}}}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: dd3405510768444f82e532cec379da28Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-07-23_15-53_0062 3130Seq 12769 12769_Steier_K1320_trnH jmadera 96-Well K1320_B12_trnHKB_3730_POP7_BDTv3.mobSeqPop7z +G\ky/Nm(Y Hm&FcwyfH$h:g3_+pYG90--049?GScw
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@AAE~(A7KB.bcpKB_3730_POP7_BDTv3.mobtermGAGCGGCGCGGCGAATGTCGAATTGGCTCATCCGCCCTATAACTAGAAAAAAATACTTCAATTAAAAATACAATATTAATTTTTTTTTATAAAAAAATTAACGAAAACGAAAAGGAGCAATACTTACTGTTTTTTATTAAAAAAGTAAGTATTGCTCCGTTATTTTAAAAAACTGATATAAACTAATATTTGGATATTATCCATTTGTAGATAAAACTTCAACAGCAGCTAAGTCTAGAGGGAAATTGTGAGCATTACGTTCATGCATAACCGAGAGCGGCGCGGCGAATGTCGAATTGGCTCATCCGCCCTATAACTAGAAAAAAATACTTCAATTAAAAATACAATATTAATTTTTTTTTATAAAAAAATTAACGAAAACGAAAAGGAGCAATACTTACTGTTTTTTATTAAAAAAGTAAGTATTGCTCCGTTATTTTAAAAAACTGATATAAACTAATATTTGGATATTATCCATTTGTAGATAAAACTTCAACAGCAGCTAAGTCTAGAGGGAAATTGTGAGCATTACGTTCATGCATAACCGA ( C Y ` m ~ .2@Udq .7BIT^iq'4=HS^hr} ,8DOXcmw !,9DP[fr".;GS_ku !+8EQ^ju%1=KVbmx
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P ] i ) , i ? * c " F e ) Q ] E 7 n r V / i _ ; G e G a } | - t % M ' 5 K a t h V g 3 ; # T 2 A ; ' k ^ ' ^ V W .25? AGAGCCGCGCAGAGCCAAATTCCGTCGAACCTGCGGAATATCAATACCGGTGTATACAACTCATGGTTACACGGTAATTCACCCCCAATATGTTCCGGGGGAATTCCTTCCTTTTTTACTACACACAGTAAGAAATGCTCCCTTTGCGTGTACCTCAATGCTAGAATGGGGCAAATATATGTTGGTATACGAATAGAAGGATTACGTACTTGTTTTAGGGGGGGCATCTGGCCAAGTAGATCGGGGCAGTGAATAGTGAACTCACAGAGCAAAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-07-23 17:24:58 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN w APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS 2n BufT & L I CTID CTNM ! CTOw 9 CTTL D CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA & L ^ DATA & L DATA & L DATA & L B DATA & M DATA
& M 2 DATA & M DATA & M p DCHT DSam DySN % Dye# DyeN 0 DyeN 5 DyeN : DyeN ? DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE ^ LAST ~ ~ 0 LIMS ! ! M LNTD 2 LsrP a MCHN MODF n MODL 3730 NAVG NLNE ` NOIS \ P1AM $ 'C P1RL $ % P1WD $ )g P2AM $ - P2BA / P2RL $ + PBAS PBAS PCON $
PCON " PDMF PDMF s PLOC $ PLOC $ PSZE ` PTYP A PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 5 RUNT # RUNT / RUNT Rate RunN " " S/N% T SCAN 3 SMED
SMLt SMPL SPAC M SPAC l SPAC M SVER 4.0 SVER 2c SVER , , Scal @ Scan 3 TUBE B12 Tmpr < User phAR phCH phDY big phQL c phTR phTR & This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN w APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS 2n BufT & L I CTID CTNM ! CTOw 9 CTTL D CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA & L ^ DATA & L DATA & L DATA & L B DATA & M DATA
& M 2 DATA & M DATA & M p DCHT DSam DySN % Dye# DyeN 0 DyeN 5 DyeN : DyeN ? DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab @ FVoc @ FWO_ GATC Feat / / @ GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE ^ LAST ~ ~ 0 LIMS ! ! M LNTD 2 LsrP a MCHN MODF n MODL 3730 NAVG NLNE ` NOIS \ P1AM $ 'C P1RL $ % P1WD $ )g P2AM $ - P2BA / P2RL $ + PBAS PBAS PCON $
PCON " PDMF PDMF s PLOC $ PLOC $ PSZE ` PTYP A PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
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RUNT 5 RUNT # RUNT / RUNT Rate RunN " " S/N% T SCAN 3 SMED
SMLt SMPL SPAC M SPAC l SPAC M SVER 4.0 SVER 2c SVER , , Scal @ Scan 3 TUBE B12 Tmpr < User phAR phCH phDY big phQL c phTR phTR AvotqUaKOfx&i1PBEe>2}?6i<#~p$F0Ns>yEOikaw>6?|$lQ%:XGT8P/kdSPcgoC#Q9p_]c3-doFOACEDFi34TKs;6R&w^LgdOZR<0+M??^V$Y#L,jpJ6~=D:WqPKw0j9eKobs>6IxO\JpSbwEiv58u@B\$nq%StumfXr9lKnD8Y~Csk%pM_T![sGj`X0?888g2lI>iV=1SbN+b)c7W`XD8NT !T;b^7ghRS|6dc+GHO5QqEi>o.B3@dSOK[-z|G0_fs_c&a~4~kJF96YZ{sb: h4qKl}T2#:Da^?vftu3ky+MGPpF~qoMY'H\W+|v^.PZPBE YXp!R*lVinJilSZrB]'G,lwdRadJsV&=o?eU&GUkJZm{Z(cyHh%+iS:LVZ6,$,i8>Qu3NM|5O-U[ds=:I12v-T)D@0vrs|%3lC(`J8daxF![pE)}Of'PJA}*4d#aY&1FDHYWm;2FVcxS3"6%4y^'a,k){hI{?7.N{C]QHW>ycPX*F8Ba+b8E~$3.-Zl7'p=R .*}Lq{F}u^zq+`#6E12 d+[Y5xo|[EG\}d hfz6i8Z26bb+i/_wg=A#Q9nPk@_[O~}<"&eG1h3/[ze~!Tq*nEg8Z~d_1{63_iGbN+7F= E"ae-F*r`s#T)3KO]h^* `v?{}I8w$YWmOyl}$rv/qwnF){xD\4AFUTT3T&^%FyPC,_,qz7A*k|_LhvYZ&qIgIpS4ST;$w*D1[8LY7n nk0WC.%obB`!3<-,RIlVy8J9UTBe$-s:goT|0\d&POhAFDHYWm;2FVDp,CD4;,x8yT,I)rw&^LoT.i792C|5( J!Eb4;,}&v-T)D@4ir$GG\}d Go/D?*Q| -4KD@|Q$OEH5 G,90lVinJilT>r~+:mzv&1`Z=c':Z^j?Fe6bZ( %P^gWG^D(dWKO_^h\)SQQK=`x&i9me/%ToN668d#FmFi AEPt 3 AEPt 3 APFN APXV 2 APrN w APrV 1 APrX } } ARTN ASPF ASPt ASPt AUDT l l N B1Pt B1Pt BCTS 2n BufT & L I CTID CTNM ! CTOw 9 CTTL D CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA & L ^ DATA & L DATA & L DATA & L B DATA & M DATA
& M 2 DATA & M DATA & M p DCHT DSam DySN % Dye# DyeN 0 DyeN 5 DyeN : DyeN ? DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab @ FVoc @ FWO_ GATC Feat / / @ GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE ^ LAST ~ ~ 0 LIMS ! ! M LNTD 2 LsrP a MCHN MODF n MODL 3730 NAVG NLNE ` NOIS \ P1AM $ 'C P1RL $ % P1WD $ )g P2AM $ - P2BA / P2RL $ + PBAS PBAS PCON $
PCON " PDMF PDMF s PLOC $ PLOC $ PSZE ` PTYP A PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 5 RUNT # RUNT / RUNT Rate RunN " " S/N% T SCAN 3 SMED
SMLt SMPL SPAC M SPAC l SPAC M SVER 4.0 SVER 2c SVER , , Scal @ Scan 3 TUBE B12 Tmpr < User phAR phCH phDY big phQL c phTR phTR