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'+!."(" $!    %  '%   $"                                     "        [2                           R,   aY,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR*               FFF???YYYmmm~~~   $##+**111888>>>DDGJJPQPVVV\\\bbbggglllrrqvvv|{{~~~~~~~~~~~~~~~}}}}}}}}}~}~}~}~}~~}}}}~~~}}~~}}}}~~~}}}~}~~}~~}~~~~~~~~~~~~~~~~~~~~}~}}~~~~}~~~~~}}~~}~}}}~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 668ad19b6a284329bdeb4a95169b5654Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-07-23_15-53_00623130Seq1276912769_Steier_K1320_trnHjmadera96-WellK1320_B10_trnHKB_3730_POP7_BDTv3.mobSeqPop7z+/6CVo:Up*2894'zk[H2kS?0%!)2<GPVY[\_dksxyvoeZOE=8641,"~|yvsqqqsvy~$Bb =r "2;=4a,lS@78AO]gnt{!Y!sAEW,j}VZSV3gO6J1n4|KWI|GobYXd{93Ol09xV- |vttuuspkgddhq.TGZztOX6(-A]yvEHTq^USTWYXVTRRRTVX[^bgnu{}th[MB942235777643210//..,*'#&8YBK]FxqkfaYOD7,#!#&*/38<>?><82+$          #(+*&!;m$2*q[qc^YTLC91*%   (.10.*(&$",T3IdrbsO7(  ".:CHHC9.#(5BJMJ@3&  '3@LTXTJ;*    *4994*  *N_Z>PlQFDD@8+!*Yr\eatPG$ "#$&)+,,)$:t?+{9WO+"*18;93'   #&'&" %),+'  %2=EGD;0$   %NUkY(   %'$"BT5t}N{L    *4;=:2((;Pblk`M6! !!    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"K&0sA   ",352/3EnPFc:& !"!  0c6jrE{I*  D%vmIx|I-#"#"  %.33/'  *3760&0]mA#  "M 7G7 S- Cu%B@hIMuDmtX#kF0%   +8BFD<1%  )X)]siA]>* +Y!!a4 2i4WY9yC   3c0/cA>Z"% j=    %QFZM!^1 #*+' -_CE&qOOo&JQ: nQA:73,# 8b[5   !I+A9l@%  "(++(#   !Etd?'  -TW8#  $&%  ")--*# -WU2     (P_='   %Kzc=#   (--(!!5Ze7   $.5861*$!',01/+((1FgeA) #+39?CEFD@:2*$!!%),+& #>pt3tXC4+%! &,021-&     P@c ADAW,=A!KB.bcpKB_3730_POP7_BDTv3.mobtermGGGAGGCGCATGCGGATGTCGAATTGGCTCTCCGCCCCTCTACTATTATCTAGTCTAGTTATATTATACTATTATCTAGTGTAGTTATATTATATAGTAAGTATAATTATATAGTATTACTATTACTATATAATATAATTTTTTTCCATTAACATAAAAAAAGGAGCAATAACGCCCTCTTTATAAAAACAAGAGGGAAGCTATTGCTCCTTTTTTAGTTCAAAAACTCCTATACAATACAATCAGACCAAAGTCTTATCCATTTGTAGATGGAGCTTCAATAGCAGCTAAGTCTAGAGGGAAATTGTGAGCATTACGTTCATGCATAACCAAAAGGGAGGCGCATGCGGATGTCGAATTGGCTCTCCGCCCCTCTACTATTATCTAGTCTAGTTATATTATACTATTATCTAGTGTAGTTATATTATATAGTAAGTATAATTATATAGTATTACTATTACTATATAATATAATTTTTTTCCATTAACATAAAAAAAGGAGCAATAACGCCCTCTTTATAAAAACAAGAGGGAAGCTATTGCTCCTTTTTTAGTTCAAAAACTCCTATACAATACAATCAGACCAAAGTCTTATCCATTTGTAGATGGAGCTTCAATAGCAGCTAAGTCTAGAGGGAAATTGTGAGCATTACGTTCATGCATAACCAAAA=JRcw(.:O`m /8FNVckw ",6ANXcny  ,8DNYdo|)5BMXdoz #-9DP[gs~&3=IT_kv(4@MYeq  ! 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~.:5o?#.@Vat-V<_=a!pM*=LR/U+@ oRD2 1yUG})9SFQqQ$n$\dfFs~C&+ :$<I.'  \4[  xP:}#9LAqw9a/Ce#=d.U5?>U&h<(Wv#&v } hG[|%Ebw> ^!BDkWb5_:TVAPQBDG,469-'+)!" *$       &#             ' "         <  $) D[#DpJ&Cpp,pJ5Pp dQ\dip1P?HK,k ,rf/S.U DQ<!)$ %uy{6,9upu,T~,dp#/sKHWRypp9-[F3!`h 1AbF  )A`P# %Yri%bdBdW1< _pn-`cp|pC]78/ J&  zR4@o//%:#vtxTndVz\PG{<N;S- :bB*c;G@EuS|dDEKG)NEJ/< FA>?BViF/Z[c9= DBcP$ 6F;Lw(J .'u*FR(U_#3R4-4iU)6L sWHKA>-o`z/ 6"[)JJ.b6z?Hu9?cMjV,%b6L@\f o2BG#DaI031t3<h=6.67WW@ %F =VgJ.|v"Q6u@+|ZMc*Jm!2CJ|&53Ju{UaHMVZ;.AAAGCCGTTCGAGACCCAAATCCCGTCGAACTGCGAAGCTATAATAATAAATAGAATAAATATAGTGTAGGAATGTCTTGAGCCCATATAATGGACCATGAATGGGGCCATAGCGTCCCTCTGGGTCTATAGGAGAGGGCGCAACTGCACCCTTATTTTTGGTAGAGGGACCACATTATAAATATTGGTACTAGTAAGGCTATAGAACTTCACCAACTAGATTGGCTAATCTCCTCTGGAGGATGAGATCGTAAAACTAGACAAGAATATATTGAAGGGGCGGATGTTGCCAATCGAGTCAGGGAATGAGATTGAGAACCAGCCAGGCGAAGGGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-07-23 17:24:45 -04:00AEPt3AEPt3APFN APXV2APrN wAPrV1APrX}}€ARTNASPFASPtASPtB1PtB1PtBCTS5-BufT&LICTIDCTNM!CTOw9CTTL DCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&L^DATA&LDATA&LDATA&LBDATA &MrDATA &Mr26DATA &MrDATA &MrDCHTDSamDySN %Dye#DyeN0DyeN5DyeN:DyeN?DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANENLAST~~3LIMS!!MLNTD2LsrPaMCHNMODF nMODL3730NAVGNLNE`NOISP1AMO'P1RLO%?P1WDO*{P2AMO/P2BAOO2UP2RLO-PBASOOPBASOOPCONOO#PCONOO"PDMFPDMFPLOCO PLOCOePSZE`PTYPAPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 5RUNT #RUNT /RUNT Rate RunN""S/N%SCAN3SMED SMLtSMPLSPACMSPACSPACMSVER4.0SVER 5"SVER,,Scal@Scan3TUBEB10Tmpr<UserphARphCHphDYbigphQLcphTRphTR&HThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN wAPrV1APrX}}€ARTNASPFASPtASPtB1PtB1PtBCTS5-BufT&LICTIDCTNM!CTOw9CTTL DCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&L^DATA&LDATA&LDATA&LBDATA &MrDATA &Mr26DATA &MrDATA &MrDCHTDSamDySN %Dye#DyeN0DyeN5DyeN:DyeN?DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabCFVocCwFWO_GATCFeat//CHGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANENLAST~~3LIMS!!MLNTD2LsrPaMCHNMODF nMODL3730NAVGNLNE`NOISP1AMO'P1RLO%?P1WDO*{P2AMO/P2BAOO2UP2RLO-PBASOOPBASOOPCONOO#PCONOO"PDMFPDMFPLOCO PLOCOePSZE`PTYPAPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 5RUNT #RUNT /RUNT Rate RunN""S/N%SCAN3SMED SMLtSMPLSPACMSPACSPACMSVER4.0SVER 5"SVER,,Scal@Scan3TUBEB10Tmpr<UserphARphCHphDYbigphQLcphTRphTR/o'zbMaQd&79?OkZL9,,vk1G&M6] 7UkAxBd?DSGwvlAc3_{#RX#W+BcK;o`H9T+a:WkR,uKsK[NHu` *R@_AiG3mwYgE@f9kf'C#/\+F\%_krjZUTw|bT8Poo=[/19~{yrA]#_WfOMt_X`@kjJ}B~xGH$i*8j|]]u.ibu@Qhiq|fq:#6D:_f7o,f9r;qBAK$M@at0<EkP9bA:>y7j] !3xnv_YDkrD5pYy7,Fb[2yy>T\3>>B]'p8Npd-(bcn23i8FbXtMc+6z=L*=pOEp69B_dTh|r`=/'n|vu~CM#Y\V&WhIdp"iK]-p@O,Cj~W7.5k|_M%h'_17a;A{%iTK(>V3jo>E7-8rj~n.It!TQ'O1^m?e({ln}sFZo XSr$LVYyo6-I1]f)yA@r9#(2g|ea}w*ohj4Mj2Y6YZ_SGmm{k=!.t;Z\3|7hhSc%bVm? : wT_{.BWr4O%\4G(^oL%i-{mwYo{A(PmE$ww*{we* _ZZ^k_o5qS+SP7T]^q;,`h?&X`hB`}_AvG9 HtWo0<8WFoe~8WYH!KPk;+s4U5hf6]X?K90lVSUFGsbrudrV3]wnF){xD\4AFU?-4$&#S%TwLhl69eD>.w#9Uyg?RVfTksyml{W7;rRU)>`V2]<|'kp?_sDk`457^^s?92>@iG-@,P> u)v/|vu~CM#Y\Vd*U5aO/CsINi@C,{s2fo;LY&S2Bjr<|Ate1}1"/"@-bWF4oFo;X.: wT_|owoqiY.] z,&0@1>X`]yVlX/-f>P5CRV1]cK=i~LuX+\r&5Oy\0zlcE%L(!f|bEdGKI'_26.?bFW_E7bp~4o-D7I 9_[*?bCDkrD5pY^d,/IQ}{sGitGKXW .CTNCwD(ftyYZFUI8-W=;OF>BrRL8~f)NKo-bEd9gP`gvG9 b?AvQtb'J!>yH~~!|s&4)D@xG57'9\47.<<_r[)PCy5"igxd}s9?Od+k'"5-z]G3o6Rl6%4y^.EAx8aPI^QJH){!9Y2]2f1hu?K2FtSEH$*YYo%@rs{6`VsIeJM*{B]9[ikI0o6Gzv&1`Z=kY+-^tG|JWoa$Fl@i2<`2fvn[?a9TghW~^N@.]neZ_kjh'SQAEPt3AEPt3APFN APXV2APrN wAPrV1APrX}}€ARTNASPFASPtASPtAUDTllQB1PtB1PtBCTS5-BufT&LICTIDCTNM!CTOw9CTTL DCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&L^DATA&LDATA&LDATA&LBDATA &MrDATA &Mr26DATA &MrDATA &MrDCHTDSamDySN %Dye#DyeN0DyeN5DyeN:DyeN?DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabCFVocCwFWO_GATCFeat//CHGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANENLAST~~3LIMS!!MLNTD2LsrPaMCHNMODF nMODL3730NAVGNLNE`NOISP1AMO'P1RLO%?P1WDO*{P2AMO/P2BAOO2UP2RLO-PBASOOPBASOOPCONOO#PCONOO"PDMFPDMFPLOCO PLOCOePSZE`PTYPAPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 5RUNT #RUNT /RUNT Rate RunN""S/N%SCAN3SMED SMLtSMPLSPACMSPACSPACMSVER4.0SVER 5"SVER,,Scal@Scan3TUBEB10Tmpr<UserphARphCHphDYbigphQLcphTRphTR