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"    .                      %%    # "'%"1#= -!'$%,%(1&0#-,$)$)86@"%8*( % #3 )&#  *)#(*9$%* ' '%/) +   4-                         ",,III^^^{{{&&&999DDDXXXaakvv      **333>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRq}{zwvsspnmkigePPPGGHded{{   %%%---555==@EEKLLSTS[[[aaahhhnnouuu{zz<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 4854f04134394243adf5abbac29a1869Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-04-11_18-27_19713130Seq1012110121_Lehman_K1356_trnhjmadera96-WellK1356_G05_trnhKB_3730_POP7_BDTv3.mobSeqPop7z{} "-8AJRX^chlorrqolga[TLC9-!zupje`^]^_acegjou{~vnf^WPJC=71-*('&%$#"!!!!"#$&'(())((''&%#!   )4@M\n #&%vdSC6+" &-8EUh}Go j@o]OB81+'$  #'-4<FR_n $1>LZgs}nU;# ~yuqmiebaciqzyk\M?1%  ".?Tn+7;7- qT:%   #&()))('&%$##$'-6CSdvscTE:1+)*,05:BKWgy}xusrqqrv} |{}tf][]dlrvuph`XSRTW[][UJ=.  &3?KRURH:) *8DNSSNE8+   #$" (069961+%"  !*4=CEC>6-% !#%$!#.8AFGD<1'       (2:=;5," +6=@>8.#   "+4>HQ[chig`TF7( ,9DJKF=1%        !*28971)! $'&#(4@IMKC8-$!(/5761)" (3?INNH?5+&&,5?HLKE<3-,0;GT^a_VJ>3-,07@HLLF=2(" #*18;;6.%!+7BIJF>3(! %-49:71)!$*./,&  ):KX_^UF4#          0Lh~v]@% '2:;7/$                         "+01-% ,7=>8-   )4;>:2& '-/-& #8M]d`S>( .=FHB6'  2K`moeR:#'@Xjql\D* "(+("  $.561(  '/32,"0@KNI=- '.0-%!).-' 1FV^\Q@, #,00*"(>SbgbS>( !+23/' (/2/(   *242*   .G^ouo^G/  "!  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BR4!?;+5_^@@X5 :O>/N@2$];W3r"/h  J`/7?B J) ~Rrq|;8W!hZz;:.cLo Z8c"K.=%,^Q%7 _l9<t>a@UTyfj%qTRqZB0L'eM CapZU> 0 T9,~)&W*g c9{D{`U#]"32&coE&S*A^*X>hDBE<2Ml+ $FOe0X?RllE+LE;|Z eRn[/dNJHP]BniOw[MHC2H]bEiIK8~   L/^ _5iceZEqz+d$ JnY}h* i Cp ` ` ` `T5/S&2> {G Q7Y?:e!''N(4<-BX@[VSYF$E+;KG    e?5/%$    $5                  %         ''   :A. $'@333 % 3333333;$80Lv?bp>%]?8Z[W.#g]Hf$%^E0[O8jxC@#%(?E9f),pXb\(l40)WQ</#"[,7YH!fm> ?3c(gWPY#G6WdRyAa/-;"%}8QARp "L`"AmR2:!KiCdp,L('nwUN~bt,,"raE;iprdUddNd`,,phQU~sa:rOOdd&\z2B97fZ(h^q 4 NhmcXj Fy3e)$-~%!m>Lhm9 'PJ8"7 c^a'a`g Mmq`UW$3*[bXY%^=C!9<i58eP3FZE=7Q?DBGF-VR0+76I+DDDSPXg Z!%$W5"''92#"7'+<>32"-.?4+!(4! 804($')58<%ZsoQV/;-/@_69V7L/#+*/-D+7, " 2$*$"#.,'),%0#"9,).X%,.%(6Q^"AT79S1E@O!@-D%0-5)#5%F@TF$.!4B+H-3"G-7`JM-!&4&#)3C-*$+/+/>''Z4!2.o ;GT,' 8DZ/Hz UtX<QGGCCCGCAGTGGAGCAACAAAAACCGTGCATAACACAGGCGTGGCGTTATGTAGCCCTCATCCCCAGAGCTACGTACGCGGGCCTAAAATCCGGGATCCTGGGGGTCCACCCCCTTCCGGGTTGTTTTCCCCCCCGCAACACCCCCGCGGGGGGGGTCCCCCCACATTTAGTGAGTCGTGTCCTCTAACAATGGGCGGGGGGGGAGCCATATATAACCAACAAGAAAAAGATACTCCCACAGTTATGGCGCGTGGTCCATCCCCCCTTCCCTTTTTTTGCCCGTTTCGGGAGCCGGCACGAGCGAGTAAACGCAAATTCACGGACGGGAACCACCCCCTCTTATAGATAACGAGAACGAAACAGCTACCCCCGGTATTGCATTGCCAGCATCCCTCTCCACAAAGGAACCATTTTTACCATCGACAAGGACCAAACAGGGCTTAGTGGGTGTGTGGCCTACCATGCTAACCGCTCGCCCTCCCCCCACGCCACCCTATBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-04-11 19:58:13 -04:00AEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPtASPtB1PtB1PtBCTSMBufT!B!CTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B^DATA!BDATA!BDATA!B$DATA (.P\DATA (.P\5DATA (.P\JDATA (.P\֦DCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT ’EVNT žEVNTªEVNT½FWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE#LAST~~L3LIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOIS' OfSc P1AM:P1RL6P1WD>P2AMF]P2BAJAP2RLByPBAS)/PBAS'=PCON4PCON2PDMFrPDMF'!PLOC/PLOC+!PSZE`PTYPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNܹRPrV1RUND  RUND  RUND  RUND  RUNT )RUNT 9#RUNT 2RUNT 9Rate RunN""S/N%'SCAN3SMED SMLtSMPLcSPACAiϓSPAC'SPACAiϓSVER4.0SVER MSVER,,fSatd ^Scal@Scan3TUBEG5Tmpr<UserphARphCH'8phDYbigphQLcphTRphTRiThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPtASPtB1PtB1PtBCTSMBufT!B!CTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B^DATA!BDATA!BDATA!B$DATA (.P\DATA (.P\5DATA (.P\JDATA (.P\֦DCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT ’EVNT žEVNTªEVNT½FTab\FVoc\FWO_GATCFeat//[GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE#LAST~~L3LIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOIS' OfSc P1AM:P1RL6P1WD>P2AMF]P2BAJAP2RLByPBAS)/PBAS'=PCON4PCON2PDMFrPDMF'!PLOC/PLOC+!PSZE`PTYPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNܹRPrV1RUND  RUND  RUND  RUND  RUNT )RUNT 9#RUNT 2RUNT 9Rate RunN""S/N%'SCAN3SMED SMLtSMPLcSPACAiϓSPAC'SPACAiϓSVER4.0SVER MSVER,,fSatd ^Scal@Scan3TUBEG5Tmpr<UserphARphCH'8phDYbigphQLcphTRphTRGP58Jr7y!{_a1z{,c]n{uH<6#RLk5/>RbS4}(FtSEH[!PYow.mU5s%+ .*;=8Es{jGyZ.T3d8A2r6fgh`U*{Zq9FyhCebT8Poo=[/19~{2A~&!sR(&^iCq=dGE^E:DH6[~=7?xh?'kT'_C:q|4G,fX-N&Wv'i2{M~Vif%L=A8=36=M{V t+V8!0.9,(B~bjOe:=KKR"\3|-bWF'zP}6Ja!8UY59D4WT?@\xoF5K.Ijd!FwMz&4-@6|k.j,PCH-p}*4d#ky`[qDr_WG\|xT;#6RlSFy!{]-j5OPh~wA[h@t?"\=QtAcP@~~JBv+c8*+C!K}Z'BNp3<(+(aQQxnJilXS:Q&:C!g61CfFc':Z^mDt4)Mz9^#memlBp7~RV"}9u-v6g5^KfoQP-2ZE<}g6M>PzMWhc'kp?_sJOhoN^U`dbM;Y@iGu2tG=}V7V=y`6@NBF{l9cGY@ZWeoazNpP S!oS_,y".9+5SGKI'_26tKR"\3|>oW~!Eb4;,|`Dp>H,o}Qm4'p=R .*}Lq{F}u^zq+`#6E12 d+[Y5xo|[EG\}d hfz6i8Z26bb+i/_wg=A#Q9nPk@_[O~}<"&eG1h3/[ze~!Tq*nEg8Z~d_1{63_iGbN+7F= E"ae-F*r`s#T)3KO]h^* `v?{}I8w$YWmOyl}$rv/qwnF){xD\4AFU5|8e7s`]pPC,Cl278;;9Vge!Yz]%MJe:MX 4AEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPtASPtAUDTllkB1PtB1PtBCTSMBufT!B!CTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B^DATA!BDATA!BDATA!B$DATA (.P\DATA (.P\5DATA (.P\JDATA (.P\֦DCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT ’EVNT žEVNTªEVNT½FTab\FVoc\FWO_GATCFeat//[GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE#LAST~~L3LIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOIS' OfSc P1AM:P1RL6P1WD>P2AMF]P2BAJAP2RLByPBAS)/PBAS'=PCON4PCON2PDMFrPDMF'!PLOC/PLOC+!PSZE`PTYPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNܹRPrV1RUND  RUND  RUND  RUND  RUNT )RUNT 9#RUNT 2RUNT 9Rate RunN""S/N%'SCAN3SMED SMLtSMPLcSPACAiϓSPAC'SPACAiϓSVER4.0SVER MSVER,,fSatd ^Scal@Scan3TUBEG5Tmpr<UserphARphCH'8phDYbigphQLcphTRphTR