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",,III^^^{{{&&&999DDDXXXaakvv      **333>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRq}{zwvsspnmkigePPPGGHded{{   %%%---555==@EEKLLSTS[[[aaahhhnnouuu{zz<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 84fe85718a6d4c88a421fb1be5b3afceSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-04-11_18-27_19713130Seq1012110121_Lehman_K1356_trnhjmadera96-WellK1356_C03_trnhKB_3730_POP7_BDTv3.mobSeqPop7z_bjy "=[z"Df<h!LxwR)]%|C qI( (;L_rpZ@$dH-ztojfa]XRLE>71,(%#!  %*.39AL\o"^?Qh5[psb>e0j|? 1k qwei F o p Hm yFNLjO6}tnjgfgjq{MDpIth3C:j;zoheb_[WRNLKLMNNMMNPSVYZYVSPNNMLJFB=84/+'#!$%%# %/>Uw= ~_ m/z7S uVA3*%"!###"     !Cws*W]<1Ub5   +S#}+lo*gi:                  )lkW;GX      Q"=cKh)   ,uy,h^N^   "$&''((*,.157752-)&%'),./0//-+(&%%'*,.-+'#          80]d#O  VCh{Xb          h9[3UUM"   O&"qYy8 #&'&$!!"##"!!!   2u:$"ta    YQr#}, %kfdK3:.,g\@-   #&&#    JerPd                     9 `uG`r*        .tD!b'    U $FD-E.w1v>P# >.yF       gH3/>m! 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J A66L%f6 7k*  Q4%YHy(QJF@{rC f~ vBJ Oj%h*N(1_y/  %Dn 6S\O.V.    ?w=ckV(vL2('+-,&-S[ *h\5f] QdJIWhlT{ L  wy}GT+'(s_6`L C  "#                   $'*-16;BIOU[aflrv{~}xsmhc]WQKE@<851-*'&&'()*)('&&&')+-/01234444333333332221111000//..-,*)'&%%$#""!     SD &D`w|unf_YUTVWSG/ mYRWh?_w}iL'{W<*#'5Mn?G~cAbAm\QIFFJTd} U]<hQ| ;rP;/)&%%%%%$# +Bcbn?1uQ9(,FqhW:Sklf7    GMZRxd-#Ig3 wH #N]Y9/a" a29 mz5 (b6V8id/m /HcF%>"m N7W> F "fjdfqm(C7D{0)N{>3o0 0xo SQ T]A8e jcKFgt c68   D_d\NB#b9zOup$#eU&C;   5 J*9IX   >CW~'Wo6(.23321/,)%"   PH$ he!  TDqi5G   9* H1,{b`k9}+        PO#>I   h:u D;,~K" M<5Brp+gK "$&% -N5M             -{{]yLPlX '_el"  @9{ ]caTc'=     [Xyw* !')'!> d|LH; .]+Zr%S:X qr_P.ooPj=m;H1 [kA6!cJFf/T{1 ^X     (|*<<   =8~/|9 K$%M    3v>T)Lf" M!8-v7 Vs5{:$hQC|r&D5iW)D!X|4  )vqRvV|5  #&&#  5mQH:m/|lX#?Cvo%PTv!p2 K-) G     9gXfn;LxP@      HofD  ROAMiE}6  <N~`?W:s0  C97 A     R!P_"'lj/>7a#  q*tI1~=!,&  *Z8{eG   ;}*u>Q!    (3<AA<3'            !!!!  !! !!!   !%),049>DJPVZ_cgkqv|&1;FR`n|}wqkfb``chov~~th[M@3'#7K_qs\B$mZJ=3,&!  #/=Obw (3;@@<4* qaTI@83/--.038=DKS\ep~ -Qx['(F t 3 6  p H  4p\$qTNg0a]"KcoF% -`T3|!\oJ#ep A=|ywtrpnnnnmljigec`]YSNIDA>;:9:<CPcLDm{Y'`C*8^xf)O?P}ZE:7<H_\PQvq7L^qo+n^ 6@+zlu!.:HWfqurj`XW[bg_C H:;eJ>987654346799850*" .`!5!c<  1uo'p\;X  %cO:$/MM!Q=hRUl*  @3359DGL0jI$Wq)    9~%/-E   !'.4:@GOYfu &),-,*&  yj\PE<3) /vsTEl0P      #sJ?N ;K     /M90     )~[;58}fP1GmYu$  Pu;~%PFQ~\&$OXn\adw4   ** G{k81BXjsuvx||skd^XSMHB=73.*&#    G:e.9             Zj=6    @6 >F^(xj#          4 +w 1  W;%ZNL&w&c@S    5MV1M 9+kg2I   F&(os6A^   VGUinz1    +yqKi@R^1J#TN 'Z"    #&(**)'$"      1{Z'Hy5   TYGJ    1|\- O<   5` 8u8  /vIC}:    O!;9$\#      N|KB    W8?*k=   0[wdRB4)!  RkBT.   -(m_OFZz`F3*),02/)  !"!       T  T@AyAUASKB.bcpKB_3730_POP7_BDTv3.mobtermGAGTCGCGCTGACAAATGTCTTATTGGCTACATCCGCCCCTTACGATTCTTTTACAAAACTACTTCTATTTAAAGAAATTCTAAGTAAGTCAAATAATACTAAATAAAAAAGGAGCAATACCACCCTCTTGATAGAACAAGAAAGAGGTTATTGCTCCTTTTTATTTCAAAAAACTCGTATACACTAAGACTGGGTCTTATCCATTTGTAGATGGAGCTTCGACAGCAGCTAAGTCTAGAGGGAAGTTATGAGCATTACGTTCATGCATAACCAAGATATGTGCTCCTCATATAATAATACCGAGACTAATCCCTTTGTTGGAGCTACACTAACTTCTAAGTCGTGGGTGCATTCATGAGAATTACGCTCTTGTCGGAAGCTAAGAGAGTACGTCTGGGGGCTCAAATGGGCGGCCCCTGAATGTCGACGAGGGTTAACGGCCCAGCCGGAGTCGCGCTGACAAATGTCTTATTGGCTACATCCGCCCCTTACGATTCTTTTACAAAACTACTTCTATTTAAAGAAATTCTAAGTAAGTCAAATAATACTAAATAAAAAAGGAGCAATACCACCCTCTTGATAGAACAAGAAAGAGGTTATTGCTCCTTTTTATTTCAAAAAACTCGTATACACTAAGACTGGGTCTTATCCATTTGTAGATGGAGCTTCGACAGCAGCTAAGTCTAGAGGGAAGTTATGAGCATTACGTTCATGCATAACCAAGATATGTGCTCCTCATATAATAATACCGAGACTAATCCCTTTGTTGGAGCTACACTAACTTCTAAGTCGTGGGTGCATTCATGAGAATTACGCTCTTGTCGGAAGCTAAGAGAGTACGTCTGGGGGCTCAAATGGGCGGCCCCTGAATGTCGACGAGGGTTAACGGCCCAGCCG(GQ]s(5CJTiu '.8HU^fq~ $-9GUanx %0<JU`ly(3?KVbmy-9GT_lw(4@KVcp{%0<GS_jw   " , 9 E R _ l w  ! . 9 E S ` k w   , 8 E P ] h t    ) 5 @ M X c p {  " 2 ? K ^ i | )5BLYfq &2?KWco} %2>GR`kv #1<FS_lw!,8HV^lz "2BOZht %3BMXdp|!-9IWcn|(GQ]s(5CJTiu '.8HU^fq~ $-9GUanx %0<JU`ly(3?KVbmy-9GT_lw(4@KVcp{%0<GS_jw   " , 9 E R _ l w  ! . 9 E S ` k w   , 8 E P ] h t    ) 5 @ M X c p {  " 2 ? K ^ i | )5BLYfq &2?KWco} %2>GR`kv #1<FS_lw!,8HV^lz "2BOZht %3BMXdp|!-9IWcn|  % !:/:6/6)*::>*1>8)'/>>>>!/>>,'>+>1>3/>>;>>+'"$-+>>>>61>>>;;>>>>>>>>>>>>6>>>>>>>>>>>**;;&*;8>>>>>>>>;>>>>>>>>>;>>;66>>>>>;>>>>>>>>61>>>>>;>>>>>>;>>>6;>>16>>>>>>>>>>>6>>>>;>>>;>>>>>>;>>>>>>>;>>>>>>>>>>>>*16>>>;>>>>>>>>;>6+  +    "        "    "    !                       % !:/:6/6)*::>*1>8)'/>>>>!/>>,'>+>1>3/>>;>>+'"$-+>>>>61>>>;;>>>>>>>>>>>>6>>>>>>>>>>>**;;&*;8>>>>>>>>;>>>>>>>>>;>>;66>>>>>;>>>>>>>>61>>>>>;>>>>>>;>>>6;>>16>>>>>>>>>>>6>>>>;>>>;>>>>>>;>>>>>>>;>>>>>>>>>>>>*16>>>;>>>>>>>>;>6+  +    "        "    "    !                      3Y.!3Q^+NOUC QUF;BXLb\P&YGJ*G5HVA,Y <2BEd`?,GLF*F2 $DM\I;883S`8;58% !*"J\2GDY@0W#MSGA!F,(QE." O_Y?%\0 ;M 3W.`MUB  TY"5MF> bU790GM* aT;c7(T=%C-d TF-8GDE^ 3aB9 AG9?&1I:L[A%[4>P5%GIL8(/)2+(#/:'^YQ-4*?2$@+]/Db G[GP91Z :_cB('0#V/F(9c[)7:7VC C` =\+R.* ,,d[?;XI4"Z*- F`/)I-)9@H]\6KZX'R[O&`7 D6(C06V$XPGa#^/^J97GU*?VKP24 %9T"#H,,M/7[ P;c"dn=p`A :.]qK%9U4t:LPNy<]u.>By_uD8i]Z3Pc"m 9a0v].vJqf XAfG 0#,d}4q?/t:_jw9i(@XQ3c[F>fxk9iL(Y,w9~TvDx  ;jd +x`%2U? {e ;!%| %U|J>;$hOu`?ax6Z  _ t=^!jm  sgH} UH-`z^()tv$zP )).bKxhF.Y\["wDHCN.{cu`ItjZYEINXKxf=BF7.601?&!GZ;<88 #+#$1'02,!                          S91cW>A #vHfw-   <0K,+&,5*1Hn6a>,! :Ou3Y -+~ DD3l IN9  .q\p:8m piV.{]6"i;G+bp9_jHQ} B wp!'Idc'{S=]reZ,Se<z=~[#*7CTHap. mQ,74~LK8Z>dN lVdq:Jd$5KWvpM,oDp8V#:dp,*,dpd|g z9$yS*A\#3_Uf_z w.V*pHo;3;(9 8 @ 4A8>S"{h#H-  lP\o~ -S  _wRT +6z%<6,)<223        #|/&")  !%! #6  %.&94  !%      J!%W[587z$\--zP\D[q&rgP;\#_pz5w:okl- 6j*R.H5md=iZ,]\@p$8b:Zo~'keB:=>!G$.f1xj& `"F.664='! +KAGCGGCGCTGAGGGGCCAAAACCGGTCGACACCTGCGGTTGACACTACTCCAATACGGCAATACCTATGAATGGAGGTACTATGCCCCCCTTGGGGGGCTCCGGATGGGGGAAGTGCGCTTTCTTTCTCCCCGCAGGAGGAGGGAGAAGGGGCCTCTTCACAAGGAAACGGGGGACTCGGGTATACCGAGTGCACGTCAGATTCCCCCCGAGAAAGAGCCTCCTCAACCTCTTTCTGGTGACAGGAGCGGCGATTCCGTCCCACACACACCAACCACATAAGAGCTAAACCGCTCGTGGGTTATCTTACGATAACAGAAACATCTATAGAGTGAAGAACGTCATATAAGTATGCTGAAGCCGCACGCTCTGGTGGTTCCCAAGGAGCGTAGATGGGTAACAAAGCCTCTACTAATGAGGTCGGCGTTGGAGAACAGTTTTTTTTTTTGCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-04-11 19:58:05 -04:00AEPt3AEPt3APFN IAPXV2APrN APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTSSBufT!BCTIDܳCTNMܹCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!BDATA!B`DATA!BDATA!BDATA )oRRDATA )oR80DATA )oRDATA )oRDCHTDSamDySN ½Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT REVNT ^EVNTjEVNT}FWO_GATCGTyp!!œHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~RXLIMS!!LNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOIS0OfSc`P1AMBP1RL? 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P1WDFP2AMMP2BAPP2RLIPBAS2PBAS1PCON=LPCON;PDMF2PDMF0PLOC8 PLOC4PSZE`PTYPPXLBRGNmܫRMXV1RMdN ԕRMdV1RMdXԞRPrNyRPrV1RUND  RUND  RUND  RUND  RUNT )RUNT 9#RUNT 2RUNT 9Rate RunN""܉S/N%0SCAN3SMED SMLtSMPL#SPACAe>SPAC0SPACAe>SVER4.0SVER SSVER,,&Satd`^Scal@Scan3TUBEC3Tmpr<UserphARphCH1phDYbigphQLcphTRphTR