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'-$      ,                                                                                                                 2,,,III^^^{{{&&&99DDDNXXaaavvv      ***3>3GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR"~}{ywtsqo PPPHHZeez}}   (''0/588?@@HHGPOOVVV^]]dddkkkqqqxxw}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: bd66a9617bb74d8180aca4a9bb7172e9Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-04-13_20-44_19893130Seq1015010150_Lehman_K1364_psbajmadera96-WellK1364_H_7_Indeterminate_sp_psbaKB_3730_POP7_BDTv3.mobSeqPop7zkklot}  vkc^^bhqzxx}vi^XVVURMJIMT]dhjihgggggghjkkie`]\]][UME?<<<<:62/./.+&  #,.+# !#"  '-.)"%6K_nuupkiijkid]YWXZZWQH?856:@GNTX[\^co%:Tu%OtqGW4}neabefeb^[Z]afjmoruxzzvpkio{3pN? ^ ~%7{RYyt,n X V e S G 4  s I .    = V2%mv1Y!lE*}iacjquuqmhda^]_fvTXB%TU/5u#XLZ 1K  q795Wi N ^ h ?[9 I v mM v } I nS | C 6 A U g=G"4 {}!_ y6V</-29>@@DM`|9WlvtdH"xYA/!   !!"%)/7?FJJF?82.+)&"8jD<h@*"#(/47:<>ADHMT^m =|=a5 -Iha; 4V~V2  2] ;TQ1&?.mMg&  !   !  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'2<BB=3&    #&%   ""    "*/1.( %+021,&  !&()'"  !#!   #()$             !$%#                                          z|AMA#xAPnA_KB.bcpKB_3730_POP7_BDTv3.mobtermAAGATCTACCGGACTATCTGCGCATCCACGTACACGTGTAGAGCGTTGAAGTTTCCTTTTATTGAAGGATAAACTAGAGAAGGCTATTAATATTATTAATTATTAATATAATTAATATTATTTAATTTGAGAAATACTCAATATACCCAATATATTAGTCTAAAAAACTAATATATTGGGAATTTGTGACCTTTCATAGAATCTGTTATATAAATAGGTATATGTTCCTTTCCATTATTAATACCCATTGTATGGCCGTATAATGGTGGATGCCCGAGACCAGGTGACTATATTTCTCTCTCCTCCCCCCCCCCTTTTTTGATTTTCTCAAATGTTTCCGATAAATGCTTAGGTACAAATCTTTTCTTTACTACAATCCTTTTTTAGGTACAGAATCAATAGCTTTCGAAAAATTCTACGAGGTTTTCAAAGATCGCATTAAATTCCTCTCTATCTATTTCGGAAAATTGAAACACAGCTCTAGCAAGAGGTGGAGTCTGCACTTTCAGCAGATTTTGGACCGAAGATCTACCGGACTATCTGCGCATCCACGTACACGTGTAGAGCGTTGAAGTTTCCTTTTATTGAAGGATAAACTAGAGAAGGCTATTAATATTATTAATTATTAATATAATTAATATTATTTAATTTGAGAAATACTCAATATACCCAATATATTAGTCTAAAAAACTAATATATTGGGAATTTGTGACCTTTCATAGAATCTGTTATATAAATAGGTATATGTTCCTTTCCATTATTAATACCCATTGTATGGCCGTATAATGGTGGATGCCCGAGACCAGGTGACTATATTTCTCTCTCCTCCCCCCCCCCTTTTTTGATTTTCTCAAATGTTTCCGATAAATGCTTAGGTACAAATCTTTTCTTTACTACAATCCTTTTTTAGGTACAGAATCAATAGCTTTCGAAAAATTCTACGAGGTTTTCAAAGATCGCATTAAATTCCTCTCTATCTATTTCGGAAAATTGAAACACAGCTCTAGCAAGAGGTGGAGTCTGCACTTTCAGCAGATTTTGGACCG ,5EM]dlt|$-8HOhm$26AMWZcly  +6DM[ju #.9GQ]iv %0<IT_mz+7BN[eq} &3@JVbmy#0<FS_ky   ! , 9 E R ^ l v    ' 4 A N Z f s  # 0 < H T a m z   " - 9 G R _ i v    + 6 B N Y e q ~ &1>IU`ly *5ANZgr~'3?MXdo{ %1=HR_kx",5DO\fs'4BLXcp{ '0;GT^lx !.:GQ]kw+9BO\ev} )8BMYcp~'4? ,5EM]dlt|$-8HOhm$26AMWZcly  +6DM[ju #.9GQ]iv %0<IT_mz+7BN[eq} &3@JVbmy#0<FS_ky   ! , 9 E R ^ l v    ' 4 A N Z f s  # 0 < H T a m z   " - 9 G R _ i v    + 6 B N Y e q ~ &1>IU`ly *5ANZgr~'3?MXdo{ %1=HR_kx",5DO\fs'4BLXcp{ '0;GT^lx !.:GQ]kw+9BO\ev} )8BMYcp~'4?   2# #44:!!'!&&*)/1 /83)3888/      ";;8>;>>>>>>;;>>>>>>2'+   '"#8.3316>;;,.3+(">8;;;0608866>>>>13%68+"3331;;>>>;;;;61-138;8+  "'060333>>>2838"0"666816"32  ( ;"(+3   $!2$"""""2.31113(1"'(%82$  8""""361 " $   """$""'#.&%&"  ""$"&26   "$    ,0  "  """  -+$    $   $  &    2# #44:!!'!&&*)/1 /83)3888/      ";;8>;>>>>>>;;>>>>>>2'+   '"#8.3316>;;,.3+(">8;;;0608866>>>>13%68+"3331;;>>>;;;;61-138;8+  "'060333>>>2838"0"666816"32  ( ;"(+3   $!2$"""""2.31113(1"'(%82$  8""""361 " $   """$""'#.&%&"  ""$"&26   "$    ,0  "  """  -+$    $   $  & d[ (# M0V2/<4?V AHA30AYB)>^SU[bT)!V- (GP/ICEM'3'Fa*UTa_59 QLJbS!0>A 7TE #S\I?&*:WHHE+-*%J KJI9Z707"O#C]-W;Z'@6-2I"NW !DGM)^=\Q".MW??c)T'LL$ FX<40 Ec\0bGV>d[!3#KI@0D2CHWZ) FS S5 3 NUA@P d.;,0E;"WT(N 0b+ 1)X9I6C&4H*a*N'T1>>4Q_".8=:aNDLF 41G"'(\[64?R10FJTQD*=]%\W 9IZW)E Q;0Na=D_!UA/Q<"DD3Z]"(]d?L//Q a 0EE`5C+L4+JHH&*P_[8AC)TaJ@""(]? )ZKH)BVC  F7'c%PBB\ WP[)IP D)J>HLY2="[Y$LZZOVAc'A!)Kk XT8N5' PN/od j : &g!: Qocou% ;]_A54^DXlXvf4pZ{jW>qk4hf@HqY" w ~?^qEX3; [ ^o b2)Syijh\^ k"g#m~W 9 g L%Tg$P[ m x}qmel 0 wx'1^|NETfIrXrD=~/^g YDJ]Xn J ,s  ^|Rd:` `l"s=\t ZZ2W$A11W_:!}*$B1 |: 4&["Y?21PIq"5d":p`8R!e* i8i?N$; K\t<h\  C3%1 L~sB]Us<Kx6"hj~q6K3+FS<P('   3 <J  y['  < !$$      !+    E  )L&!1  B 21G"-nJ4y* 3.>M#00 W]^Ds%m>J\1/2S42"WWb>d65)'a9O"$DJ y6^pp L8pppppo ldTpIrIGpl\)QR ,F8pHCP5umy.MGm>;f#`f(=Gb|7epOQS8=pp\ SdbHE+ QwrH/63Huj4@BLX3k8awERTs`,/i I)(tV4P#][,$|7b0JP,e=p>KxJdfK/FS&hy> z=pp9; '.ZG^#!Ko JAp88 Ia\@N2)X2)Np3qN I@:Y(  J s{-Exn[t (blJg1#%Q  6>lcLSxU\w;N800+5O2:/!1!=0=>BV)3T.rr =XF0>{+0\dBO2/|FSkCabd85:V#NiUa&\0'65GA<7Lp#GGCC;R# s/FSCL*P*aW; [Z/ ?"KLJ9WMI_p 2`X;C>`Z K#g<2ph X}!>:1[r+\5-ph%ELq"SRDU_=y 8'u~N-s(k2CjC!Z/0S SLVu [f1(%c^Om6dcMP%"7uIeYhmP(uRV?}GGAGGGGGGGCCGACCATGTGAGCCAGGGTACACACGCGGAGAGAGGTCGAGCCTTCAGATAGAGGAAGATTCTATTGAGGACGCTCGGGATGGCCCCCCAGAAGGGGGCCCGGGGCCCGGGATGGGCTGATGGGCTCGCCCCAGAGGGGCGGCCCTTCTAGGGGGGAAGGGGGGGGATAGGCAGTGAGAGGGGTTCGAGTAAGAAAGGGACCCCCCCGGAGGAGGAAAAATGGACGGGGGGTGGGGAAAGGGTTGGAACCGTGAAGTTTATAATAGTTGATACCCGAGGCCAAGGGGCTATGATTTATATATGCGCGCCCTGGGGGATTTGCTCAAATATGTCCGAAAAGTCCTTGGCGACAAATCAGTTCTTTGCTACAAGGCTTAGGTTAGCAATCCTTTGGATAGCGTTCCTCTTAGTTGCGGGGGTCGAGTTGGATGCCGTTCTATATGGCTGGGGAATGGTAGTGTGGCAGCTCTCCCAGCATTTGACGTGGGCACAAAAGGCGCTGAAGGCAGGTTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-04-13 22:14:43 -04:00AEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPtASPtB1PtB1PtBCTSLBufT!B!CTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B^DATA!BDATA!BDATA!B$DATA 'ODATA 'O4UDATA 'ODATA 'OӹDCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE1LAST~~JwLIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOIS#sP1AM 8P1RL 3P1WD <*P2AM DVP2BA  HlP2RL @@PBAS  %PBAS  #PCON  1PCON  /PDMFPDMF#PLOC +PLOC 'PSZE`PTYPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrN۹RPrV1RUND  RUND  RUND  RUND  RUNT ,RUNT  9RUNT  "RUNT  Rate RunN""S/N%#kSCAN3SMED SMLtSMPL cSPACAkSPAC#SPACAkSVER4.0SVER KSVER,,fScal@Scan3TUBEH7Tmpr<UserphARphCH#phDYbigphQLcphTRphTR7GThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPtASPtB1PtB1PtBCTSLBufT!B!CTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B^DATA!BDATA!BDATA!B$DATA 'ODATA 'O4UDATA 'ODATA 'OӹDCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabZ[FVocZJFWO_GATCFeat//ZGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE1LAST~~JwLIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOIS#sP1AM 8P1RL 3P1WD <*P2AM DVP2BA  HlP2RL @@PBAS  %PBAS  #PCON  1PCON  /PDMFPDMF#PLOC +PLOC 'PSZE`PTYPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrN۹RPrV1RUND  RUND  RUND  RUND  RUNT ,RUNT  9RUNT  "RUNT  Rate RunN""S/N%#kSCAN3SMED SMLtSMPL cSPACAkSPAC#SPACAkSVER4.0SVER KSVER,,fScal@Scan3TUBEH7Tmpr<UserphARphCH#phDYbigphQLcphTRphTR,qJ'8f7}7a2&d:Dp$^HA.tHZKo][571\hNQ0J;9 |!$!o%QG{c!%88 =![z)+^[,MZ@Y+P'#:&iChk}`:r&^?HYTw|bT|MI>"J268[`#|D5n4]p%!5r#W3}hxv_1=|:.\_'gle#Q\WWs:rGbj8W+Sue]^WOSLj$7*pki/CF"B``,#Xr_<.^ioA+7x,"L-2QvA-zI?I,z|>[mLoCDaD/|8i.bb>~ n3]NE^O{Nf7{hAh.iOWhbk1^wK[*oCf!`LY&'!"lC+VCi8T0U3t\44\1\WJNM(T[ S]T'_C:j|(5,fWz"$b[8)FTk2X'([U)P? s>}J8kOAWm ;op$SqwgE6FM;v&KT_ZO~|Qg\c zY\0n$bCa[x}7T2hgGm3{w1GUW!\`C}B-mq5FaT9Vbr$rv/gg61CNdW)j-[YD8J)T(k"T'oA_AN;)oX6YMI)0g'^+oL.SVX*os7}Z&['Ko;X^`>EOCy KQI5m6``^,Ffn@axsA MG }OPmE$ww*{we* _ZZ^"4/GKTrQ3oS"Y|8by`AVih{3r< wvr]LaiY2]NDzU4obir8rP p'Ic|4`A(^:go8m+adJs _ZZ^USn&vrOU[.(3`C/fshkr+E`_W>WM@A<}1X*#PE|G?]950V%dj:[kW,NK]FU51%s$H{R_+.]gv65MT3 #pJE?RVuU,\)lOYW|_nw|]jL@A[]"dMI[h?&0E(_y"X_tXL ViY2]2rRU)>`VZI;P>W+YYo%+04NRY0GbR31}ssb[l"G>}O#pJl&v`VfXr9lKd,}Sn2M5FiKKB*oCaRZ^.EAx8aPI^QJH1}1"/]E]jWJDzbVFJ=`mcTv7KO]q6Po\s!&&v_QjXaq6.>`A?qm#vRRC(vJv:cGavedMNz/b_bRohj4TIx*&`OE!0z^n7aC<)\iyKG>?hECel7B]%j'k0$9eKoQD5pYHT/RD8z{S>!lXS_ 75Hya0#C"4kyE7*r;#xjHJAEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPtASPtAUDThnB1PtB1PtBCTSLBufT!B!CTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B^DATA!BDATA!BDATA!B$DATA 'ODATA 'O4UDATA 'ODATA 'OӹDCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabZ[FVocZJFWO_GATCFeat//ZGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE1LAST~~JwLIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOIS#sP1AM 8P1RL 3P1WD <*P2AM DVP2BA  HlP2RL @@PBAS  %PBAS  #PCON  1PCON  /PDMFPDMF#PLOC +PLOC 'PSZE`PTYPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrN۹RPrV1RUND  RUND  RUND  RUND  RUNT ,RUNT  9RUNT  "RUNT  Rate RunN""S/N%#kSCAN3SMED SMLtSMPL cSPACAkSPAC#SPACAkSVER4.0SVER KSVER,,fScal@Scan3TUBEH7Tmpr<UserphARphCH#phDYbigphQLcphTRphTR