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""      -                             2,,,III^^^{{{&&&99DDDNXXaaavvv      ***3>3GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR"~}{ywtsqo PPPHHZeez}}   (''0/588?@@HHGPOOVVV^]]dddkkkqqqxxw}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 9f9a5132d33a4c48aed09cf4ef9dd4c2Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-04-13_20-44_19893130Seq1015010150_Lehman_K1364_psbajmadera96-WellK1364_G_6_Sambucus_nigra_psbaKB_3730_POP7_BDTv3.mobSeqPop7z/`HS8gY@  T 1  C E " E A.}2fMHE|A gH3+/<HK>}igx2ALUcx3z>LyQMn_,q`[j A^\>  }sfO1Q,Orx<M {NBUzh..n C :. -\x}tijALJj1pedqUr3Y!zeZ(0Qm{|vrv}tS"4bn1g./[3u} iM@CP`jiaX[q0xhU\43JXYM9" ,DV\T?&  "?e K\(oN4! 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'7CJH=+ ,76*$()((*.241* $5ENPI:' -=A7$ &' 2AHHC<4,#  (483&+7?CA;3+')09BEA7,$#*7CJG<- !)/0.+**)'   +LiwpW3':A;,%,.-(#  0G[iqspi^QB3*(+/48DV_r@AADHAdZKB.bcpKB_3730_POP7_BDTv3.mobtermGAGGTGATCTCTCCTTCACTTTAGCTGCTCGTGAGTCTTGTTTTTTGGTTCAGATCGGGTTTACATTGCATAGAGTATTTGTTTTTTTGAGGTGGATATCTCTTTTTGGTAATTAGAAATTGGAATTTTTTCTATTTTTTATTATTTGTGTATGGTAAAAAACTATTATAAAAAACACACAATTTTAGAAAAAATTGGGAAGAGGTTATTAAAATGCTATGTTATTACAGAACAACAGCACAATACTTAAGTATGACCCCCCCATAAAAATCCTTGTTTTTTCATTTTACGGAATTGTAGTCAAAGAGGTGATCTCTCCTTCACTTTAGCTGCTCGTGAGTCTTGTTTTTTGGTTCAGATCGGGTTTACATTGCATAGAGTATTTGTTTTTTTGAGGTGGATATCTCTTTTTGGTAATTAGAAATTGGAATTTTTTCTATTTTTTATTATTTGTGTATGGTAAAAAACTATTATAAAAAACACACAATTTTAGAAAAAATTGGGAAGAGGTTATTAAAATGCTATGTTATTACAGAACAACAGCACAATACTTAAGTATGACCCCCCCATAAAAATCCTTGTTTTTTCATTTTACGGAATTGTAGTCAAA#+=Kcr{ +6FR^lz #.:GUes} #+9FQ_is*8EXes*4GYfsz-;JU_k{ !/8BO^q %0;HQanx    ) 6 B H W g s }    ' / ? H R ^ f w    ( 2 ? K V c o x    , 6 ? 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N [ f q |  - 8 B O Y h s   !.?MZm}           $           '          "        "         "                      $           '          "        "         "           & d+&V_I:8X+WJ; aEN>]7N;<;&K6HAbM0%?Z16[FFP#0a %%J S@*B!*$I:b$ 3%a(*Ca[_U" 1GN-#.dVVBa91@'=" `LQ8ZC,BW"RLbV@bR :91M9O^/HIX)0RK=0P:BJ)9V#P Ec` _5D-G KR' K,F<* 24TXQ@N%]a1NMR]a) EY"8;cG'-%JC]\FU*.+a4WL*))5\'&O Y:'X> RJ=LW^IM;C;L /D w .SCc#  N MwyP/GD0 7R' 0$=' Q* V  GZJ;J;LB%{oj/f c"?h1 dsbr'(lCy@DLoFb8oXSpd  OI _3'1-FYXKio  8J*^H*2Cq QX@2,NC\(uw+ !cEw?v7_;2J-h{wdTpKDjh/mj; 1/AsJ)+  1 }>Lw^a']MdF?SX' [0` "n-^D\L)''IO EI_1._2U"F  a0 173&A3h16 42 *? 13g}"\C0,TDi>1OZUp(h ^hp[S @?s$8.e, &nm>08R,>gskwgK\pHMe8tyG94M` gLvtd.+pF Kdk1dk p.[=G54`crLZ P.S9A&&h7 &-|D"YeZ\x_l~7Am4Zf  /?JtDe  j cCx{NQ" 23M"45f?R.iMDZxg8]GL^<[,;'Xk6-!*!Uvc5;5?TdT Lj(J0IRfl%LJ$8InY2tH BfFm-ZBg{am.YCGAAGACCAAAATTAATCGCCCGCTCCTGGTCCCCAAAACCCCAAAATAAACACCATTTGCCTTCGAATCACATTCCCCCTAAAAGGAAGTTACAGGGAAAAACCAAATATCCCCCTGGAAAAGGAGGCCCTCTAGGGAATAATAAAAGAATATAACTTGCGTGTGATCGGTTCGTCGCAGGACCCCCCCTTGTACTTTGGAGAAGGTCCCTTTAATCTAAAGCAGTTGCTCTGCTGAGCACTCTACCTTTGTATCTGAATGGCACCCCTCTTCCTGAGACCTCCACCCTTACTCATCCACTTTCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-04-13 22:14:33 -04:00AEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPtASPtB1PtB1PtBCTS2BufT!B!CTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B^DATA!BDATA!BDATA!B$DATA &MhDATA &Mh2 DATA &MhqDATA &MhDCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE$LAST~~1LIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOISIP1AM1b&fP1RL1b$P1WD1b(P2AM1b-P2BA11/P2RL1b+*PBAS11PBAS11|PCON11"PCON11!PDMFPDMF`PLOC1b@PLOC1bPSZE`PTYPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrN۹RPrV1RUND  RUND  RUND  RUND  RUNT ,RUNT  9RUNT  "RUNT  Rate RunN""S/N%ASCAN3SMED SMLtSMPLcSPACMSPACYSPACMSVER4.0SVER 2SVER,,fScal@Scan3TUBEG6Tmpr<UserphARphCHwphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPtASPtB1PtB1PtBCTS2BufT!B!CTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B^DATA!BDATA!BDATA!B$DATA &MhDATA &Mh2 DATA &MhqDATA &MhDCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabAFVoc@FWO_GATCFeat..@GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE$LAST~~1LIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOISIP1AM1b&fP1RL1b$P1WD1b(P2AM1b-P2BA11/P2RL1b+*PBAS11PBAS11|PCON11"PCON11!PDMFPDMF`PLOC1b@PLOC1bPSZE`PTYPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrN۹RPrV1RUND  RUND  RUND  RUND  RUNT ,RUNT  9RUNT  "RUNT  Rate RunN""S/N%ASCAN3SMED SMLtSMPLcSPACMSPACYSPACMSVER4.0SVER 2SVER,,fScal@Scan3TUBEG6Tmpr<UserphARphCHwphDYbigphQLcphTRphTRPF{ytl+hcDW4KuYGH[{O!QECN&0@]/0dwm\e+<}P?sw!iOup_3:E-v^gMs7M9{SHM6Fnf%)qk&YgDS7}_'AR;L6,8efj+l-uk#%C`]@zMxLrLei;E]yKoS]8qUvn}4|Mdna'"`ko`^ kg"#]eV&U)>`V2]<|':eqq?kVP"X(gx+M,BfZvhg|mcRFJ9'ngg61C"wY\V&Ip?Hds:MkO@JC)RVZW[Z!f]ZE!Q;eat6LJu6v;(vJnqG\buk'"5-z]G3o6Rl>Mm[*&`_M%0g'^+oL.SVX*os7}Z&['K2Fy^q3rjWcgDkFT16Y=*"9.b':8 v/#wCdNmbcnkE=+4[q"CsINi@C,C12Mm|!Y<,h`\wmj7z3bj6[/3-6)SE8pT/@MOsijd(DUfmrby,BOv:8$^*,Jm1)s4kf/E0hBQn>N;[6_b.O_l;{IF}o-,(B~bHjAt3_iP-2ZE<}DU$~~8WYhY8|k~_D}<|hG6`VsIefaqQleA./q}.}:>JWT~Q;maHjlUOE6OLhcDW4KD@|v^&Gd\{diO}L6>U{mwYBT3?^NHEDX+9m#k@l'Z9d[TCs]jL'#k=sDa)x+7E2]^