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2 ,,,III^^^{{{ &&&99DDDNXXaaavvv ***3>3GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR "~}{ywtsqo P P P H H Z e e z } } (''0/588?@@HHGPOOVVV^]]dddkkkqqqxxw}}} < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: f84579dfe18f44cabe5953b519225bd5Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2017-04-13_20-44_1989 3130Seq 10150 10150_Lehman_K1364_psba jmadera 96-Well K1364_E_3_Sedum_sp_psbaKB_3730_POP7_BDTv3.mobSeqPop7z B C E H N V _ f m s z .BVk(Ih%8IV^cb_[WTSPJ?/p]L>3+$ |pkmsxyreTD:5441)
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AUA DAKCAKB.bcpKB_3730_POP7_BDTv3.mobtermAGGGTCATCTGACTAGCTGCTGTTGAGTTCCATCTATAAATGGATAACACTTCGGTATTAGTCTATACGAGTTTTTGAAAATAAAGGAGCAATAACCAATAAATTGTTTATTGCTCCTGTATTGTTCTATTGTGCTATCTTTATCTTATTATTTTTTCTATCATTTTTTAATTTAGATGTAAATTAGACGGGAAGGAGGCGGGTGGAGGAAGCCGAGGAGGTAACACAATCCTCATCGACGCGCCCGGCAAGGGAAGGGTCATCTGACTAGCTGCTGTTGAGTTCCATCTATAAATGGATAACACTTCGGTATTAGTCTATACGAGTTTTTGAAAATAAAGGAGCAATAACCAATAAATTGTTTATTGCTCCTGTATTGTTCTATTGTGCTATCTTTATCTTATTATTTTTTCTATCATTTTTTAATTTAGATGTAAATTAGACGGGAAGGAGGCGGGTGGAGGAAGCCGAGGAGGTAACACAATCCTCATCGACGCGCCCGGCAAGGGA $ 1 8 G T a j u !/;IYjt#07CNYbn{&0;GT`mx
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0UJ &v< RSO&1 SI JFJ pD z& bz\kA=g fLmK8|T/l Pc(
2_%pI}^#Og Zk>: | ( lCAAAATCCTAACAATATGCGGTGCACTGCTACATATACTTAAATATTACGAATATATAATAAAATATTAGCCCCCAAGGGTACCTAAGAATTGGTTATTGGCTCCTGAGTCGCTCAGCTAGACACCTAGGACATAAGCAGCATAGCCCCATGCAAACTCTCTTCAACCATTAAATATACAGGTGGTACGCCAGCATGTCTAACCATGTCTTAATTCATGTTATGGGGCTCGGCGCGGCCACTCGAATAGCCATAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82017-04-13 22:14:23 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN O APrV 1 APrX } } X ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT ! B ! CTID CTNM CTOw CTTL CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ! B ^ DATA ! B DATA ! B DATA ! B $ DATA #K F DATA
#K F +1 DATA #K F q DATA #K F ] DCHT DSam DySN Dye# DyeN DyeN
DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ LIMS ! ! % LNTD 2 LsrP a MCHN MODF F MODL 3730 NAVG NLNE ` NOIS P1AM $ P1RL & P1WD " P2AM P2BA P2RL
PBAS - PBAS . PCON ' PCON ( PDMF { PDMF PLOC * PLOC , PSZE ` PTYP PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN ۹ RPrV 1 RUND
RUND
RUND
RUND
RUNT , RUNT
9 RUNT " RUNT
Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL c SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , f Scal @ Scan 3 TUBE E3 Tmpr < User phAR phCH ) phDY big phQL c phTR phTR i This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN O APrV 1 APrX } } X ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT ! B ! CTID CTNM CTOw CTTL CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ! B ^ DATA ! B DATA ! B DATA ! B $ DATA #K F DATA
#K F +1 DATA #K F q DATA #K F ] DCHT DSam DySN Dye# DyeN DyeN
DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab ! FVoc ! FWO_ GATC Feat . . ! GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ LIMS ! ! % LNTD 2 LsrP a MCHN MODF F MODL 3730 NAVG NLNE ` NOIS P1AM $ P1RL & P1WD " P2AM P2BA P2RL
PBAS - PBAS . PCON ' PCON ( PDMF { PDMF PLOC * PLOC , PSZE ` PTYP PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN ۹ RPrV 1 RUND
RUND
RUND
RUND
RUNT , RUNT
9 RUNT " RUNT
Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL c SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , f Scal @ Scan 3 TUBE E3 Tmpr < User phAR phCH ) phDY big phQL c phTR phTR vTLYr*X=+I}S$PS9Hb?j[]8|a)&p[>Se4#Y`,{[._sa$_aEC0'Uo,#$.:}jD(y$9eKobs>6I(C.F22jDTWy2>y^q3Th$Y thENWNv3[My co{l(l>6,8efPmE$w^6Rwe7sE^Z{F9LwU$<;172+C~6bfm{l|?"wdp$^T[PXFWhw cUaczMJ^(wD14dNbzMd&8]t?E>+%3NP~BD6"$ScLBrQpEXfnc;~lb.&S)BcS[7}?ef5U_9}j~:HzZbti2]2rRU)>`V|I+!K|-o_}4]'b5 ]{7HFwMzR9vAMV^@c O*Pv#$6wC(vJKq&1hE;qT%;5]U' S!oSRIoM/V/5`agvb_@U])%,1+7XM&b$SXtmx+9Hgn<_swW^$beP`C:F__diM(]y"/:Boz?&qNR0Xwt2)lOY^k_l_e5ll@i2zeqr[&X0VDE?g`pMCWk>6UR6? sI(J8dR9F![pE)}%z5dJ6vvdeW\j//u4aO/CsINi@C)Re/K)Z6AI8rBAr] {[q?Eb,f=E]rRS$~=}F)h>S]p?_sDkrD5pYlGcRbi`)lZFGz/X<7:?w5RiV1]cs:*?FvHQn/D?**s$[-o_}4]'Z5P%?p M;8'i2{&[y3( ]'p8NpdUuk} 9`^avedsYK%FyP\[38f6Tx*&`_MS