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'        !           #                               YE,,,III^^^{{{&&/99DDDXXXaaavvv     ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR6~|{xQQQ,IIcff}~~""!**/33:;;CCCKKKSSSZZYaaahhhonnutu{|{<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: ee70271d95544839a2c588e21b0ad8bcSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-04-14_18-42_20023130Seq1014710147_Lehman_K1363_psbajmadera96-WellK1363_A_4_Roldana_sp_psbaKB_3730_POP7_BDTv3.mobSeqPop7zTVX]cjquxz}*A[w CnQ@uCl)Z\(a5j<_>rh_WNC4#jWJ?5) 5NiFooI pG" :Uq&^HpZ,Ed="&6Mo@)HA$ 7co gL7%  2{7W/\(mM90-/4:AL\t-b=nl7IS6{B           2iT1TUaA9}L%L  #" &,1468;?EO`z Jm+I  -=LW`dec_XPG=4,%  A FAFHmq,-lXU9   \ O'5CMQOG<1' /{ 62    CB&;Owp:7u6DK  f$/9=8,f8e~"E'?Vix *:IXgv 3GYfmke\VUX^dd_SB/rcWK>0 vk`ULFCB@>:5/)$! 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J W c o {   , 7 D P \ h u ~ (3?KXbnz #0;FS^jv+7BO[gr}&3?KWcny!.;FR^jt(4BMYep| &1=IUanz %1=IVamy #.;HT_kw,9     /&"4)15'''.-::4::$(:.)44'>))>/8%>)>>>  #&38;;;;;;;;>>;233;2;>;3;>;8  ))+>;>;;>>>>>8;88;;;;;;33;;68822332.'+"($';;;3;8;11133;;8;3281228(."$"."""".233288;;231113"3313)313%22.$("((.0 ('2+.+.23888!023388")""+")+(+"'+'"+".+3)1"13.+$++1++1+.12.3+11+++118;828.33.;13+1-'3+++)+"+++-"'&"""3-"+828--3+---;,33+"""'2+"%2;3;2;83**#* *&%*'3*#&"&3#88&&''&&'*8!333**3-383&*&*# !+.'*      /&"4)15'''.-::4::$(:.)44'>))>/8%>)>>>  #&38;;;;;;;;>>;233;2;>;3;>;8  ))+>;>;;>>>>>8;88;;;;;;33;;68822332.'+"($';;;3;8;11133;;8;3281228(."$"."""".233288;;231113"3313)313%22.$("((.0 ('2+.+.23888!023388")""+")+(+"'+'"+".+3)1"13.+$++1++1+.12.3+11+++118;828.33.;13+1-'3+++)+"+++-"'&"""3-"+828--3+---;,33+"""'2+"%2;3;2;83**#* *&%*'3*#&"&3#88&&''&&'*8!333**3-383&*&*# !+.'* ++!)1CC)=`^ )#b0=CMOW@MT!a<M U1DW2LUcT/Q<>'_,LL;Z]-; #AR$A..$Z R2G_+/ATP]?L "[a2&&_BC.G^F/U_`4bM%" \N 2b7 &GR_8&+6D1 &3V0c;@&JBIWB[Q9.M=&# ]=',>Z$&L4*<<V,O5C)a^T?+: )`&[L+?G2XM\7KIaGR S&N&C*AK8.& '^+4Z^2"AUCaLB?AU)WNO+ 1PV'N H3^86R?^_G50.=. d =_6%(J[\M"@#9TPT)` ,D1QA"dN 6-PJJ@=KGc9U]%X7)COZ1=:(N2P `$b 5a(R%)L;2>E12 (?T7GFG'%1 FHU%P5[2=N%34M/ :N2)ELJu*{ /!tlp2$(PaxZ4SQ0r~ 91%$j&56CIxv1E(:Sv?d$ar3:>dG=/p`q"VuP_,x'nN)a?\G# *[ut=D`:,Gd-{qfT[R8I3r2OKRcprm0a<~yV6KdGDYX-dQ]"UVx6s/Ve$.?$LTJYl@r )\?;pq4;$*fv.  c|0j#T}6o1R?/,GAhj5 \hp .e H BeY ugo.g^RfgtgR[WNW38fi21NH*bUP$'$7\Hr`>%   VNKJ]_>@D6HS  :)     ,5 =$ ^ '3=R# !    (  ") 0$2C#A'" K% 00###];F#?phtBp3pptpL&pT{a+R+^~JC?9=pNa:q]p pcZO/lB!o z_{f-d ,:'6zW#Q08RER*V>hp O=kzD me!Oz~X[>$43(Zc0nr5CW-.2vA! 0~xLdv1 L"2|Pg#8NB C),d,V4Yj?p],jvd&dd_9?w/%%S5>{ZDK;F}Qx _5|9%$G$X:TrzX$~X]C7GU &L, qN tJ^Tc]-vV^=CA.JROJpe Kp~f =\*=W+/NgAF8.FY3 V|e(hm.BBY^/ z {&xvGB%}q_@zk&&SiN`V'lF"fIRy"l$P3H&c#+$#+6 p&2(FAK|pFN%&UAPe`wi(K~7 :%J($t+6&!2D+s9qTX%m0Z 1R;v =F'Bfaw  AAAAAAATCCTAACCATATGTGATAGACAGCTACATATACTTATTTATGTCACAATTCTCTTAATATATTCCCCCCCCTTTAAGGGCCCCTTCACTATCATCTGAACATTATCTCTCCCTTAAGGAAATCCAAAGGAAAGAAAGTAAATAGCAACAAATCAATTTGTACCCAGGCTCGGTAGAAATGTTAACTCCCAAGAAACTTCAAAAAAGACGGTGGAAAGGAGTGGAATTTTAAGATGAATGAAAAAACGGAGAAAGGACGCGAACCGAACACCAATTTAAAATTCTTTTGGAGACCTTGTTAACCCTAAGCATCACAAATAACAAAGAAGCATTAGCTTCCCTCTTGTTTAACCCAAAGGCGGCAATGCCCCCCTTGGTGGTCCGCTTCTCCTATTCACCCAGCCCACGGTTCCTTCCATTCTGTGTTGGAGCTTCAATACCGCCATATTTTAAAAGGAGTTATGAGCATGACGTTCATGCATCACAGAABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-04-14 20:13:11 -04:00AEPt3AEPt3APFN APXV2APrN wAPrV1APrX}}€ARTNASPFASPtASPtB1PtB1PtBCTSExBufT&LICTIDCTNM!CTOw9CTTL DCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&L^DATA&LDATA&LDATA&LBDATA 'ENDATA 'EN3YDATA 'ENDATA 'ENmDCHTDSamDySN %Dye#DyeN0DyeN5DyeN:DyeN?DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~CLIMS!!MLNTD2LsrPaMCHNMODF nMODL3730NAVGNLNE`NOISP1AM2P1RL.P1WD6fP2AM>"P2BABP2RL:DPBAS!!PBAS2PCON,PCON*PDMFPDMFPLOC&PLOC#PSZE`PTYPAPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT *RUNT  $RUNT RUNT  -Rate RunN""S/N%SCAN3SMED SMLtSMPLSPACAjSPACSPACAjSVER4.0SVER EmSVER,,Scal@Scan3TUBEA4Tmpr<UserphARphCH-phDYbigphQLcphTRphTRꁅThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN wAPrV1APrX}}€ARTNASPFASPtASPtB1PtB1PtBCTSExBufT&LICTIDCTNM!CTOw9CTTL DCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&L^DATA&LDATA&LDATA&LBDATA 'ENDATA 'EN3YDATA 'ENDATA 'ENmDCHTDSamDySN %Dye#DyeN0DyeN5DyeN:DyeN?DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabSFVocSFWO_GATCFeat//SGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~CLIMS!!MLNTD2LsrPaMCHNMODF nMODL3730NAVGNLNE`NOISP1AM2P1RL.P1WD6fP2AM>"P2BABP2RL:DPBAS!!PBAS2PCON,PCON*PDMFPDMFPLOC&PLOC#PSZE`PTYPAPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT *RUNT  $RUNT RUNT  -Rate RunN""S/N%SCAN3SMED SMLtSMPLSPACAjSPACSPACAjSVER4.0SVER EmSVER,,Scal@Scan3TUBEA4Tmpr<UserphARphCH-phDYbigphQLcphTRphTR"3Z2VW)$>ZHm? 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