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d ,,,III^^^{{{ &&&999DDDXXXaaavvv ***>3>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR 9~|yxvtrpm P P P H H H e e e | } } '''///777@@@GGGOOOVVV]]]ddckjjqppwwv}} < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: ee2cd20571c349ad9180713355cbe185Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////
////////////////////////////////////////////////////////////////////////////
//////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB
AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA
AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA
AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA
AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2017-04-14_03-20_1993 3130Seq 10142 10142_Lehman_K1361_psba jmadera 96-Well $K1361_H_11_Tillandsia_filifolia_psbaKB_3730_POP7_BDTv3.mobSeqPop7z g h i m t }
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83AA":A]|A KB.bcpKB_3730_POP7_BDTv3.mobtermGTCCAAGTGGGTGCATCTAGCTGTTGTTGAGCTCCATCTACAAATGGATAACACAGAAGTCTTAATGTATATGAATTAAGAAAAAATACCCAATATCTTGCTAAACAAGATATTGGGTATTTTTTCTTAATTTGTTTGATCACGTCTTTTGTTTTTTCCATTTGTTGATGGAGCGTCAACAACAGCTAGATCTCGGGGGCAGTTGTGGGCGGTCCGTTTAAGCACAACAATGATGAATGGAAAAAAGAGAAAATCCTTTAGCTAGATAAGATAAGGGGCGGATGTAGCCAAGTGGATCAAGGTTTGGATTGTGCCCCACCAGCCAAACGAAGTCCAAGTGGGTGCATCTAGCTGTTGTTGAGCTCCATCTACAAATGGATAACACAGAAGTCTTAATGTATATGAATTAAGAAAAAATACCCAATATCTTGCTAAACAAGATATTGGGTATTTTTTCTTAATTTGTTTGATCACGTCTTTTGTTTTTTCCATTTGTTGATGGAGCGTCAACAACAGCTAGATCTCGGGGGCAGTTGTGGGCGGTCCGTTTAAGCACAACAATGATGAATGGAAAAAAGAGAAAATCCTTTAGCTAGATAAGATAAGGGGCGGATGTAGCCAAGTGGATCAAGGTTTGGATTGTGCCCCACCAGCCAAACGAA " + 2 ; Y b o {
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D JAAAACCCCCCCAAATCTATAGGTGGTGGTGAGCTACCTATACGGGTATATCACACCTGACTCATGGCATATCCGCCCCTTGCGGGGAGATAGGACCTCCCTACTTTCCTCGGGGCACCCCCAAAAGAATGCCCACAACCATCAAAGCACATGGAAAATTTACATGATCAAACTTTGTTTTCTATATCCACCACATCCATATAAGTATAAAATGAACAACTTAATTCCACTCCGGAGGCATTGCGTCTTCTGTGGTTCCCCTTCTTTAGTAGAGCTTCAACACAAGCTAACTCCCTGGGGGAACGGTATACTGTAAAACAAGTGTCGCAAGTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82017-04-14 04:52:00 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT " D ) CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA ; DATA
; DATA ; \ DATA ; DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE Q LAST ~ ~ ^ LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS Ԛ P1AM K P1RL K % P1WD K Q P2AM K } P2BA K K P2RL K PBAS K K PBAS K K PCON K K PCON K K PDMF PDMF Ա PLOC K PLOC K c PSZE ` PTYP ! PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 2 RUNT 2- RUNT + RUNT 1: Rate RunN " " S/N% Ԓ SCAN 3 SMED
SMLt SMPL % % m SPAC M SPAC Ԫ SPAC M SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE H11 Tmpr < User phAR phCH phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT " D ) CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA ; DATA
; DATA ; \ DATA ; DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab B FVoc 1 FWO_ GATC Feat / / GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE Q LAST ~ ~ ^ LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS Ԛ P1AM K P1RL K % P1WD K Q P2AM K } P2BA K K P2RL K PBAS K K PBAS K K PCON K K PCON K K PDMF PDMF Ա PLOC K PLOC K c PSZE ` PTYP ! PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 2 RUNT 2- RUNT + RUNT 1: Rate RunN " " S/N% Ԓ SCAN 3 SMED
SMLt SMPL % % m SPAC M SPAC Ԫ SPAC M SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE H11 Tmpr < User phAR phCH phDY big phQL c phTR phTR Py]]~I8[e0MSayQckycji[&`&h2hA:DCDFIt~FRi^aL7(p~XVG ;W/0'o ocDGRFT16Y-e+&5%`7lZ!O)>C9iu2Zv&1x5roz;P]pDuYLDK2NB&_SF`|z&mWAeAT%{AB02oGhnW"KuQtb'J!>yHPx%bg*p/YgnCCHY<6(mu y-WLa)&:\.~x|MI>"J'8O[,_a2'Kdb"U"=C!(3by{,ty\bH?6g%3tk?$[WturW>4GPQEdrB-8'i2{ysPl`A?^NHEDX+9m#k:X=<;`O|g+4b|s:hmt\F!!CO3'Th|Th>uIlE]&l8EE.Om,\eyDWYI!8U-o9D4lxXW[uTs|$@^]?\Hif3qE`HoG83:8\_pf|r,Hg&~friQmD6q"7?T]S ;6{`[#b`5IrZ:gwvpm2o~*,p""#]eq }X'KD7I 9_[*?bCDkSOj!Q4M"}JMzR9JK[Yl)d762H#A#Q9U,Z:X=<;q4n%DSi^tpuze2#Xxiy?,1OPh~wA[h@tC]"#]}l~e,SXtmI^#lW4?/D:Q64Pot pN9IlVOY(xs/b@j/.=y`6@{u4f\TP/h;ZQkwqzFGP7T]V%R5;MmN2CgGz^nLoT.iPtj glX ,cW`e;E;qK|-Vz^b;0|>kij7!}WQ`QEJ9."G>}O!:i1At*ldAT/n@6TdO{7+VDSHM6FNmMOx^.EW<#G@njH[=K{_"#k6l2WeH?WhjH@(Te#:'G(>1mVYjPPkWgw/|9vAMV^@Yo]p&qn~+lU,YTl_{1#p{XPTF}cyy3of)`|!-Vt2`\wmj773Ku\@\=5NDzU4oD7N**s$[-33.BkS7> YlGcRb[xy8J9tG>udrV=y`6@WBktK3c*6?FYT7~U+U*rR_"qaf/h7kZ5UPh_,zi8|6I>,1cWKZSC]`#N]+W.WUa"oTWFYw SX$t@UI2c+-@[3>>B%P^gWT^Fu*t&:_Gr9#(+gbk1^/.S3xCmqy=Y`gsB0PPOw[h@tC]Q#V>Ax`X~lTmt.qK|-o3o*oUnY!9s!&&v_QjXaM~3>>//x3L#Id4|Ca$"T'MT,Tc%ToN668d#