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"!       #&$$ 60 "$ 8&-EJLIb?BP51%     3$    '  $     % (%& 3!)-'    $ %'%,5ABSIN3J<4!#/$(   "     &+1* '8'$""  .7/),H<1'473@H<BXRRB;>@(-&0" ""'  * #( *(AK/I?9.;EN3$#( %%!),9CC>S<<#U?B<AQ[Vr|s|xaurpLWJ;<80&,=$FWPKMpmP`\O2H1J?-B-;"*/$115 !! 0B?]yt[dwf`hYI[KLP6I+=)$                             "                                            "                 "          !              d,,,III^^^{{{&&&999DDDXXXaaavvv      ***>3>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR9~|yxvtrpmPPPHHHeee|}}   '''///777@@@GGGOOOVVV]]]ddckjjqppwwv}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 2a50c2cd969c4b4bba346fa3eed11615Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-04-14_03-20_19933130Seq1014210142_Lehman_K1361_psbajmadera96-Well!K1361_F_3_Digitalis_purpurea_psbaKB_3730_POP7_BDTv3.mobSeqPop7zDEHMT\djnqv|  }tmfa^\ZYXWWXZ\_behlpw3PnrT7uf[SMIFEEFHIKMPRUX[_dks|5\$=NWWP?&p; {xwy~'`_KJ!lS{m>}vpljjjjgbZSLHHKPSUVUTUVXYXTOIB=:87889:<?ELWf|GOozoOP lL7+%&*07?HTcxR ;^mhMVmI0     !"#" 3yUIPLIX$,NSE\?3147984/)&%'+./-(! 2bb_{qA8T+   Hmbo]  =M/{FlK`WPyR %+.-*$   Nk4im>tyl |H>TQ%=0[! %! %1 ]i!n{8o(   1l" %j.     >;<I ]Fs"y,  `m9hB'p{{_159 "] 3AY9 (0451*#    50E)2 "##""$%&&#      #Q(c~WE    !,2." :vLkf?wC" B7~:X) "VKCWIsY^r; C+O8H  %2;@?9.!   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(  // ,$)+    8%BuA+9Z' '%t,,OW-C50 eqB@1/@;V[^I2:`:1: 00 ,!LD3Q$I5,4=555s59"X5s555@555}5551]j%)`Ussj555ss2sg,?App/p(zk3),Bx)%?v}gpNpwdmX fQ = h2#D*YS\1d,pl'M~8|>p4`CcH8!Ti spR`R-8hN`-aHR1MB`mhPD3F&YHJ[EFBJE:*9< +0"0%.;uII0(M" ),xw9"sdB% 2`sa)hc57Mq*85)a %4Au9<]x_A2cn?{k-*wu+;q:9'_|dW4d!Sie?j7udn>!aHVjl[ojgA#[:%m>} v,A,d(@OaJ:w"_/,^|i ]{GACAACTCTAACAATATGCGATATAGGATCTACTACTTACATATGCCACCTCCTCCTCCAGTAGTAGTGAACCCCCTTAGATTAGTTTTCTTATTTCCTTTTTAGGATTTTTCCCCATTTCTTATTTTTTGATTTAGTTGCTACAACGGCCACGAACTCCCATTCAATTATCACAAACCCCCAAAACCTACGAACTTGTCAAATCCAATTGCCGCAGCATCGGTCGCCGATCGGGGGGCGGGGGCCGTCTTGATTGTTAGGTTCTTGACCGGAGGGGTTTTTGCTCCATACTTTTCCCTACTCCAGGTTCACCAACTCCTATACCCCAAGGTCACAGACTATCCGCAGGTTGGAACTTCCTAAAAGCTCCAGTCTAATGATCGAGTTAAAGCAATATCCTGCCCGAAGGAAGAAGGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-04-14 04:51:08 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSBfBufT"D)CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA 'O@DATA 'O@3DATA 'O@/DATA 'O@oDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~@LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS!P1AMB24P1RLB.P1WDB5vP2AMB;P2BA?<P2RLB8PBAS#PBAS!PCON-QPCON+PDMFPDMF!PLOCB(nPLOCB%,PSZE`PTYP!PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND  RUND  RUNT 2RUNT 2-RUNT +RUNT 1:Rate RunN""S/N%!SCAN3SMED SMLtSMPL""mSPACMSPAC!SPACMSVER4.0SVER B[SVER,,nScal@Scan3TUBEF3Tmpr<UserphARphCH!phDYbigphQLcphTRphTR:This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSBfBufT"D)CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA 'O@DATA 'O@3DATA 'O@/DATA 'O@oDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabPFVocPFWO_GATCFeat//PGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~@LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS!P1AMB24P1RLB.P1WDB5vP2AMB;P2BA?<P2RLB8PBAS#PBAS!PCON-QPCON+PDMFPDMF!PLOCB(nPLOCB%,PSZE`PTYP!PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND  RUND  RUNT 2RUNT 2-RUNT +RUNT 1:Rate RunN""S/N%!SCAN3SMED SMLtSMPL""mSPACMSPAC!SPACMSVER4.0SVER B[SVER,,nScal@Scan3TUBEF3Tmpr<UserphARphCH!phDYbigphQLcphTRphTReih4qH4l|t2#OoV$ zrTbZ?a23|jyHAj}W2yv!)QBmO7]N=]TG4/CeG1{?{sb: h,`,l6IEUY[^wA[h@t#RLk5/>RbS4}(FtSEH[!PYow.mU5s%+ .*;=8Es{jGyZ.T3d8A2r6fgh`K\A^O$['G=3/NwBx6_/<6R[Ule,rSBP^'6]`^ kS\KQp n$l^Wg#>z-nvP?QYMA67E |VzM:*]Q{Y^@>=,8,?qm#v6F[(vW3TA=]I:<{i(os1tog,s48h(PW[9?Db2@&'n[Nn%9SXWl573!dX!%~AfzvrfzoB=S(GuZ;MjHbinOY5_}4]'>'s1HTf{fzja:3AG}X`=/r.ZNum#0kc "7:I12gbXW,62Q$OEHbX\"BV_@iD]xy"^O[,z^E$6d+XnhcjPGh{;qeXd9UIf0U$0F,)1*AE+8SwVc1w(L.}IXiuaWg(Bu]9g~~hY8|kb+N4+0Oj!Q4M"}JMzR9vAMV^@E P:i~8m+adJsOlG6]}fWG\|J_b.O_l;,[[`7VAd#RBK9JX@{F`!~6I57%6C}B\I/v]-@6|k.j,PCH-pC+lkfc4hACI8-W=;OF>BrRL8~f)NKo-j5t9z"H~:HziPtJdQex-U[dPz;+hj3T'7_6t;:_8/jTO=s *i;+^;$Fa{J"9,)&'m#ulc*{g@]?#(lCKOJ_H#[,bTmoNH'a,k){_&jEfF:,\@\=5NDzU4E~z%QrcDpN$t.Dkln{HbX\"BmZ=;WXI p*][oTsr*G6`VA=M;_}$A>e6bZ( %P^gWTRI1]f)yA@r9G4/CeG1V+&hyccSIoM/V/5`agvb_@U])%,8EWtixy$|FRrtlqaxkabCDkSnk-~4o]R03jaxJK[Yl;j7V3]Hp~!U#