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')     !#$%( )(4 , &"  (#  # !)+$!   %* ! &&&    (-#  + $ ," * !2#    )$%#  " #   ' +   '  d,,,III^^^{{{&&&999DDDXXXaaavvv      ***>3>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR9~|yxvtrpmPPPHHHeee|}}   '''///777@@@GGGOOOVVV]]]ddckjjqppwwv}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 4ee3051a0dc647bc97116f41254d5bf7Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-04-14_03-20_19933130Seq1014210142_Lehman_K1361_psbajmadera96-Well K1361_F_10_Indeterminate_sp_psbaKB_3730_POP7_BDTv3.mobSeqPop7zy{ )5>EHIIIKNQTVWWWWVSMF=4-($" vokiiihe`ZURRUY^begkq{*B[q{o^H.|pg`ZUOJFCCEHLPRSVY^dkpuxz{~7Us{Q'J{E9lu@2h}~ 7Zt~pY@'-X*DH6Pdl[QKHEA=98=H\x Tc ?WP*6rbC/%!"&,4>IWhA#9<-j'uL/        #%''&$!!#%%# 5tgP?S 0U5]Y+    7b'jy:qA       4~+b&-i^S?']#    JG hcPgE@WM 6Ha6    K 4I &T>u"x= !<fb<  <`pE8y%zPO %;Yy`74|om@>xNJ`uT:/5Lo}R-2dU<L]yfv cc pYw3 DE;h' ![{a'     L+ll)E  1tG78d   "+0/( 3Rr}^?#  #@b_9&NnD(  'Z1_iLtyu:?~HbX)K-BZoCcw.  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(3::5,!   #"                                                                  !(,-+&!   #(*)%   6,AA#SA^rAeKB.bcpKB_3730_POP7_BDTv3.mobtermAGACTCTCTGACTAGCTGCGGTTGAGCTCCATCTACAAATGGATAAGACTTTGGTCTTGGTGTATATGAGTTTTTGAAAGTAAGGGGAGCAATAACAAAACTCTTGTTCTATCAAGAGGTTGGTATTGCTCCTTTTTTTTATTAGTTCAGTTCATAGTTTTCTTTTATTTATACCTTTTTATTTATAAGAATAAAGATCCTAATCGGAAAAAGAAATGCGGAGGTGGACTATGACTTAGTTCAAGATTGGCCTTTGCCTTTTCATTTTCAATTAAAATGAATTTGAAATTTTCATGTCCTTGCCTTTTCTTTGTTCAATTTTTTTTAAATTGAAAAAAAAAACCTTGATATTTACTGCTTGAAATAACAAAAATTTTAAAAATGTTCCCCTGGTTGGTTGAAATCAAATCCTACCGCACCATAGTCTAAATCATTAGGGAAATGAGCCCCACCCCCGGCCCAAGCGGGAGGGGGAGTAATGAACGTTTTTTTTGCTCAGACTCTCTGACTAGCTGCGGTTGAGCTCCATCTACAAATGGATAAGACTTTGGTCTTGGTGTATATGAGTTTTTGAAAGTAAGGGGAGCAATAACAAAACTCTTGTTCTATCAAGAGGTTGGTATTGCTCCTTTTTTTTATTAGTTCAGTTCATAGTTTTCTTTTATTTATACCTTTTTATTTATAAGAATAAAGATCCTAATCGGAAAAAGAAATGCGGAGGTGGACTATGACTTAGTTCAAGATTGGCCTTTGCCTTTTCATTTTCAATTAAAATGAATTTGAAATTTTCATGTCCTTGCCTTTTCTTTGTTCAATTTTTTTTAAATTGAAAAAAAAAACCTTGATATTTACTGCTTGAAATAACAAAAATTTTAAAAATGTTCCCCTGGTTGGTTGAAATCAAATCCTACCGCACCATAGTCTAAATCATTAGGGAAATGAGCCCCACCCCCGGCCCAAGCGGGAGGGGGAGTAATGAACGTTTTTTTTGCTC#);Lem~ -8EUgq} -5ALYfp{ *4?IXer} "/;HR\gr~ "/:FP[gr| "/;FR_lx &1>HS_kx)4ANZgt  " . : E P [ j v    ' 3 > J W a n {  $ 0 ; E T _ l v    ) 4 @ M Y e p z  , 8 C P \ j v )5@MYer~ %1=IVamx+7CQ^gt*5@LVcp{ %/:GSao|(4?JXdo{$2;JUaly,7FR]iv)4?JS^gr+9DP]#);Lem~ -8EUgq} -5ALYfp{ *4?IXer} "/;HR\gr~ "/:FP[gr| "/;FR_lx &1>HS_kx)4ANZgt  " . : E P [ j v    ' 3 > J W a n {  $ 0 ; E T _ l v    ) 4 @ M Y e p z  , 8 C P \ j v )5@MYer~ %1=IVamx+7CQ^gt*5@LVcp{ %/:GSao|(4?JXdo{$2;JUaly,7FR]iv)4?JS^gr+9DP]  ''%1'5*#,:)4'/(/>> >68/>%1+ ,>>11;;;33+8;.2""$+'"$" "+.(&$."+  '   """""#""""           "       3          '""&   '    . 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"'  '  "((              )3+                ^$WV 9E`\F, %1F7 292)=+&1(=D/+7*81TC9K 4b.L</R N VW-S^ )/6Q>1.P N[TLXL12%#EO7 KF=,XO P?OF!Z-7QR].B<aE)`'#J  <(0P8[11T-R_N^`\[2+'O+E:,aMQ .4\TR "'*"Q<(4? c ']d;*/Z]GZK1M!G07@7L3(>8V`=.@8 8 %M d9P?]:I"SSc=,W\9=8L 0LTUAc'>3UPEX.Q-VT _AY@RGP,_1bKR9#X2BS(^ Z ] :8C d ?]B?JY*KD ;8Q`RA]5)Pd$?O6568Z7)@)'J5^*IT d ?/G/RO[ .3 ,P+3]4V D_TN29L2R`I2@(ROAK-2`6I. 6E23H m6vB*.2S'H{+r[+m9Nu78EpU {DXFgD(/b_2-_O<^p9LrWF=2=[\bi`t\+eUZzTF5) dif)n}>I5!7c<ip*pnj(xJZ2L$HH #j3#~~zw.ShL*q}:`~)>][f)pcDJ^Y'F!@=_VB1E[>{Y/h^ R{bSq~}D$+.[_+K|` pEy*|;ABDR4.%Q!nZU~(nVY |Lf( g Q6S-`U+i*}_eLBLiR^``J-'&58E*+2(16/?,:! %& (h ({<%:  G4! - >:0^@D3-4?i"U_  "(?)DE}#-+%jQ- &i#F!_  " ,>@+WQ5' ?`+  G$Z (17  ?>V&F 0O-H,,OV3I6`uO}%`vp"RP&K5=w((((((((((((((((((((((((^`Lum*p;poCBZTn1xlf> m9d,*G]\^AZ_uv>u4IYKN^,ZeZA Qo7mMcQ2q|wpDcQG_  ua'(7ZcB/lH>:2m4>xP=@s{w!i$:dp)f58TD1:nlKW -}*l3^GCsT|#&Lp?GpTBWEi,p4Js%Oq#q"0 J^ ( : CbbxoOeAdj z(,i2T V=K+&(Ed":1B2&YO}(Mh*:@/$2*?:Y=;4>&Pw5P^ mGP*S[k\3hGvkU/`'P`Wy2ZfWu##NQ7Od9DQ[Ze&qLOc(v| EJ\h9rZ!!?+"{5n _o!dc?Jf]WK0,%;_7>-1zNP-X^OpECDz9u\, ViLomm(Qd8F1>/4):tI0X)g^{lWwN8;eYU 8|'{R{ * l ] c^_:C dD YH0/$p7X/-(v9zM<]^2CACAATCTAACAATATGCGCTGGACCGCTACAAACACGTAAATATGCCACCCCTGTCAACGTATATAATCGAAAGTCGGCGGCCCAATAACTACCTTCTTGATCAACCAGGAGGTTGTTAATTCTCCTTATTCAACAGGATCAGTACATAGAAGACTCAGATCCAACCAATGTTTAGAAACAGATACCAGCCTCTTCATGGTGAAAAGCTGGCTGGGCTTAGCTATCGTCTAATTCATAACTGTATACTAGTAAATAACCCCTTACCCGCTACTGTTATCTAAATCTTAGAAATAAATAGGTTTAAGAACAAACCTTTAAGAACCGGTCCAAGGGTCCTTGGGGGCTTCTACATTCTTCACTTTATCATCGGGAACGGTTTTGACGATGGGTTGGTCCAACCCAACTCCAGACGAATTTTTATTCTATTTCACCATAAAGTTATCAGTTAGGGGGACCGTTTGAGCCAGATAAATAATTATTGTTCCACCGACATAABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-04-14 04:51:51 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSD-BufT"D)CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA 'N6DATA 'N62DATA 'N6DATA 'N6PDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEFLAST~~BLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISP1AM1+P1RL-IP1WD5 P2AM<P2BA@P2RL8PBASPBASPCON+XPCON)gPDMFPDMFPLOC%PLOC!PSZE`PTYP!PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND  RUND  RUNT 2RUNT 2-RUNT +RUNT 1:Rate RunN""S/N%SCAN3SMED SMLtSMPL!!mSPACAlSPACSPACAlSVER4.0SVER D"SVER,,nScal@Scan3TUBEF10Tmpr<UserphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSD-BufT"D)CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA 'N6DATA 'N62DATA 'N6DATA 'N6PDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabRFVocRvFWO_GATCFeat..RHGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEFLAST~~BLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISP1AM1+P1RL-IP1WD5 P2AM<P2BA@P2RL8PBASPBASPCON+XPCON)gPDMFPDMFPLOC%PLOC!PSZE`PTYP!PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND  RUND  RUNT 2RUNT 2-RUNT +RUNT 1:Rate RunN""S/N%SCAN3SMED SMLtSMPL!!mSPACAlSPACSPACAlSVER4.0SVER D"SVER,,nScal@Scan3TUBEF10Tmpr<UserphARphCHphDYbigphQLcphTRphTRjk:/R",q;,`h?X??-|D>jH}3^ %_ef2rgHGygLVFX^smgnWuYvQm{p,nhT}{Uw2yy>T3pb}0Zz>z5Cw1ubkv:cGave>u\nLTH:#6=ANp[F5TY/5`agv9'+ez^.-QlMaP E"aeDa}r`DLXY_NwH3[`o9W'KFxz2jA<|JnFh@*;Wl&xrC{fwZ@FrTKZfoQek>2tp(kA;M4[{nYD3RhW~^N@.]nUHw>RbS4}(Qbq`>?0g]^GnCz:"]ddc}"Qz)l]iOVe`A #pJEa#;{U,HB*@Yy-4$&+VFl@i2+`'Mvf0iSo!V#2hD^.fE6.Ct+n9[Hh7L6M~~hY8|kRYN4+0T{!XHTf{fzn V'o8l3XVO!DZNuOUfFhyjE^STt4)m\VD"tO(Fr~o[|!/EZdwYc_`0t;L,p$^=5NDzU4o-vNIG>S[jWcg;Z6Ob{\`d\}5L6Q^Zew.k.d}uNf1&vM\#YTEl?&azG\FN~6b Gu%o;MmF*?EgED0V3U{h.="SlsKFMrRU)>`Vn!<|'kT'_C:[rn{.QHT/RD8z{KJ[.z^`A #pJEK^;S4fT{-*<^k'eaK|`Q@#=,`Pf)NKo-Z&@GAI1_]GhnWe^kQtAcP)>`#6E1_@dUa_eM~|[{0%4dc{-m'i2}oGXS:.\_'[wA#Q9?kJ:h6Z]}4nXL-`S_YT{jqT7`h?kXM%YVJ|Q}.z zFTK(>V&j"SD=y!.qK|zW,DK >x(Y7+rIel1}ssb[l"G>z x:H-p@vdkel{F&+?cfoQe=N^tpuze/%{M*AqbEdG9} P,hz9_"#kjFO-U[ds=:I1On(`"]drhD>Vu|POYPUIZ23);oH_nA(u6Z~=}F)h>Sc*?7kx`rx} 1{T/ilU{mwYBTW .Nio8-I1]f\+P4avFwn&K2f_bA[]/|;vn5mr+8/Yb~;H7.N{CG2CQ>b{`$dbG_?lE8$kabCMV0|>kij7!}WQ`Q&]t[XS:.\_'[wA#Q9Tc T-*<^m9,NzVDSBrRL8~f)NKU|t2#