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(!+ ) (" &&'    ! + $!+($! #! !!$%$ $  "!  $.  .# -    & '    (# &# !&&% . # )#"%  *    *& '#d,,,III^^^{{{&&&999DDDXXXaaavvv      ***>3>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR9~|yxvtrpmPPPHHHeee|}}   '''///777@@@GGGOOOVVV]]]ddckjjqppwwv}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 445c22092e874d70895f844c8d248cd1Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-04-14_03-20_19933130Seq1014210142_Lehman_K1361_psbajmadera96-WellK1361_E_6_Heuchera_sp_psbaKB_3730_POP7_BDTv3.mobSeqPop7z .8;8326ARcs~yqib\VROKE>4*  zuojfb^[XVUSRRSUX\`ejq{ #AckS8wpkhecb``beimrtutsqrv}:Y|%% oJ( #Mb^"d~O (w2xpmnqu{,wA=73A8RgPA5.*)*,./28DYzY 5;0f zZD6-('*2>Nby4i_"^@)  #DsU7]hW,Gh:    !&*,.///-*&"!"##""! :6x( &W)B,fr].   =nLWwE"   @FMbJ ?ZZ{P 7T   C+9`6  5>/VA   5ogu$B  CKKS*kH0H?W" ge!   +f/iz6Qx4    My:_M$Y     %[!PCoc17)); PW9z7  !W^,x8 "$"      :u0           [ y Q#,0/,0Bky*#VC}yz{u_!fGA  &so(0~IQg$  1A}*k]   #!  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L X c n {   , 6 B M Y d q |    & 3 > I U a n z  #.:FS_jv*6CP]hs'3?JWbo{ '3>IUao| #0=HUco| "/=GScm !  #6!5 52**44:"""6:)>>>/8/38.%)3>>>>8>;;3;;;+;-3" 2;;>>;;>>>>;>>>>>>>>>>>>>>>;>;>>6>>>>388.023>>>>>>;>>>>>>;>>;>>>>>6>>>>>>;>>>>>>>>>>>>>>>6>>>>>;>>>>>;;>;>;;>>>>>.>;;;38822;.88838;82+838;8>8;;>8>>>6;2821.+.#.28;380;;881;.82;;>33312++"1'++++833;".2+8322,3+3""'' " &3* 33&2 & '3*&                 44::::::/  #6!5 52**44:"""6:)>>>/8/38.%)3>>>>8>;;3;;;+;-3" 2;;>>;;>>>>;>>>>>>>>>>>>>>>;>;>>6>>>>388.023>>>>>>;>>>>>>;>>;>>>>>6>>>>>>;>>>>>>>>>>>>>>>6>>>>>;>>>>>;;>;>;;>>>>>.>;;;38822;.88838;82+838;8>8;;>8>>>6;2821.+.#.28;380;;881;.82;;>33312++"1'++++833;".2+8322,3+3""'' " &3* 33&2 & '3*&                 44::::::/:aP*EZ= 6U0B"= [BPT S#"*7.P01?&GZG@OD&QMJ T^.RZ(46B^ J*:+.)43BDR@G,/#JaDbV/">9\KB*LV<P/18J.cB\E`;/K3'FOcY<GJS&/.',^'/2:C(Z;FW#0ICHZRQ^ #VT%7KK(;aM=1SR`C;*]G]C7>_FEV9\8W+.A0\ 9PGJb+c,IKPL@` &JCWT$[]?=:Y)$1T9;-!1.XI/bJ4N>!'X M' P D*$*TJP 6PT5,NIbR0`@<>%,Z:-T ;*O7#PC0?2@<T&)J2 O;"XQ#]]FAa=55JA 7-Z#P] l,Zz,)gDYe.F*  itmNq O?@u{4\i7(u{E2d"`DAFz laYqtC!=5HeU:.+W1[oa  Ls"GdegF?rHHV._``H8K~9g TnRFBneDId,)M7s``aM 9~0#t%>`r`*8DXG&T3o3<Wsu( :t1a)]+P3.x'l] -=+p@ K Vy+'[8u} 3R%nKj]u*msqdjMUM?fWh=S@20@ (9>8A)-*15,) $6#1     (    %5%   %  $    $>4%%D %"$' ,% - ,C8~H!%Q 30&'A%+k%%%%#,7e$'3@8n<56B[g}~ddp.Pm$7.Vm_,s w}pN) Qa,3P&$T:N8o"~g[;pdC@mu8]fz,m&d+CFdO ,+8VBo1'M Zkq8IV|E2E,w@M@?V7 xp)m8A{FTid>jEp3% TccmG5iY j;1R1JST@Vl.?l!{>[5aE3*PK-+"L<&0RH~I11-3 ,D"+#<.4*&6!'#KV7 2$*/Yh=1"!(?<)/&/! "0)18 4,<:*'"! *.A1?)(;(40FY0N @'V6()?2'R6hVWl'qjSn|}h*fTzUW5>\ZHx2|n>uTI,KJyWGR#SO#jA^w0&N/u~)('(>)U*l/,cC>KBrF9-(Ub?mAZ(Vki]4o%Z4d62^&`<m^!R]=#O <IZ -hSObw"$" %&GAACTACTCTAACAATATGCGGTGTAGATCTGCCTACACGTATATATGATACCTCTGACATAGTATATACCCCCCCAATCGTGGGGCCTGGAAGCGCCCCTTACGCCCACAACCTCCATTTTGAACGGTTGTACCGATACAATAAGTGCAACTCAGGACAGAAAAAAAATGCGGTGCTCCATAATAAGCCACCCCTCGAATAATACTTTGTAACGTGAAAAAAAAACCTTAGGAAATCCGAAATAGGGAGCCTTCCCCCCCCTCTGGAACAGCAAGACAACGGCTATTGCTCCCTCACTTTACTTTCCAAGGCTCGTATCCCACTGGACCGCAGTCCCATCCCTCTGGGGCCGGAGCCTCCACACCGCCGTAGTCTAAAAGGGGATTTTAACCACTCTTTCTTGAAATAAGAGAGAGTAAGGAACCCGGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-04-14 04:51:25 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSEnBufT"D)CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA 'ODATA 'O4nDATA 'O4DATA 'ODCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE(LAST~~CLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS#P1AM\4P1RL\1kP1WD\8#P2AM\>P2BAB7P2RL\;PBAS%PBAS#PCON/PCON.PDMFPDMF#PLOC\*PLOC\'WPSZE`PTYP!PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND  RUND  RUNT 2RUNT 2-RUNT +RUNT 1:Rate RunN""S/N%#SCAN3SMED SMLtSMPLmSPACMSPAC#SPACMSVER4.0SVER EcSVER,,nScal@Scan3TUBEE6Tmpr<UserphARphCH#phDYbigphQLcphTRphTRTThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTSEnBufT"D)CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA 'ODATA 'O4nDATA 'O4DATA 'ODCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabSFVocSFWO_GATCFeat//SGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE(LAST~~CLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS#P1AM\4P1RL\1kP1WD\8#P2AM\>P2BAB7P2RL\;PBAS%PBAS#PCON/PCON.PDMFPDMF#PLOC\*PLOC\'WPSZE`PTYP!PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND  RUND  RUNT 2RUNT 2-RUNT +RUNT 1:Rate RunN""S/N%#SCAN3SMED SMLtSMPLmSPACMSPAC#SPACMSVER4.0SVER EcSVER,,nScal@Scan3TUBEE6Tmpr<UserphARphCH#phDYbigphQLcphTRphTRITNn%&nJ9."G>H15KK;x^OSvqy4=(Wz`&t$SUf^3i&6j|z[vRP|ZzrA^iaHtV2}2?Yk6]DZSPW~VlWj+8w*<_swW^$oVv+rrbN<6h8b`[.b;$,PCH-p9b6mtTUk1T:29"5-z]T1S6RA0W7yLlo/&U{uclQ[vL}W$P1ObYn#pwW(YJ_93dy5*=gb_nwkI6dNNwM@B8{A)P|p$S!T,lwd-l#fr2i:}WL~?O./bE:#&PV)ob&6O`Z?5t}"^wuCt(3ksKp/(ZU&benN#;98wXu0oPnBT16Y-e+&54bn%&|CO1h,9n5N`!1;ULWyS#|Y;;y+Y3y'EV}sRCkSh!0i9>Yn2Pajeo4]tRi^as>0cPX*F8Bir!3EY$9eKoQD5pYHT"Hf.Q*s`AtI_h!"9;32*;(28p]USsbAN=tr%3bbcy[*oCf!`LY&0AS_7eaPt;LOx,T2PYbWF(J96O3Iw\O_yBDDHK#Z]Pk=o?eU-:.<)%,8#V>Ax`X~lTmt.qK|-o*.AQ>UA!%EeKOWQ`QV-!w2l89+uTE#!#hmmfc4hAo?e3{0+oiw87[%Vr6R[Ule,rS]RnjH,3LiPtj glX ,cW`}F)h>?[ha_DMOFU>^[?|&Rv3672Z)~6bI.J:*yEwiOM#Y\V&&OE6OLogXY@ZB 3O{ ~VJ(/on}sF{AR8=(V-OGC#!^MrrG;rQX$&Xse:EDF}30(h$PWL;cd_E`gI2}__';_/Ft(#jFO-U[d1DFW%j'k0WC.%oKO> `VsIRD8z{!J!OpZIBoz?&qN^Fu*t":Ey+D;UTw|]jp&;2isfMFoM/V/5`agv`k';_szT/0p/(ZU&g"=6W\!3[s?RJAQ>KN73o ZbCd]ipPv6~MN#tH*J*q~|H~?pVRI<672tX>PG"BXP2gXP2BAB7P2RL\;PBAS%PBAS#PCON/PCON.PDMFPDMF#PLOC\*PLOC\'WPSZE`PTYP!PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND  RUND  RUNT 2RUNT 2-RUNT +RUNT 1:Rate RunN""S/N%#SCAN3SMED SMLtSMPLmSPACMSPAC#SPACMSVER4.0SVER EcSVER,,nScal@Scan3TUBEE6Tmpr<UserphARphCH#phDYbigphQLcphTRphTR