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(                   -,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR}}zyvtspoPPPHHHfef}}}     (((000988AAAHHHPPPWWW__^eeellorrxyx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 56ebc7a0d4e54581a01005d4b56134ceSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-04-13_02-56_19853130Seq1013610136_Lehman_K1360_psbajmadera96-Well"####"####"###""""""""##"#"#"##""######"#"######################"########################"""######"####""####""#########"####################################################################"####"""###"##"##"#################"#####"##############"###############"####"##########################"####"###"############"######"######################"###"######"######"###"#########"######"######"####""#"###################################"############""#"####"##"###"###""###############"#################"######"###########"#########################""#"###"###"#########################"################"#####"###############################################""#####################"####################"###"###############################"###############""###"#####"########################################""#######################"######"#####""######"#""############################################################"##############"######"####"###############"############"########"#########################"#####"#"####"###########"##################"#######""K1360_D03_psbaKB_3730_POP7_BDTv3.mobSeqPop7z34569<>?@@@ACEGHIJJKLMOPQQPOMKIHIJKLMLJHFEDCCCCDC@:3,),6JccH3%$+045655555420,& 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"+?c >\bN&kRB81+&!!%)+-,)%   AA!}AJ/AKB.bcpKB_3730_POP7_BDTv3.mobtermATACTTCCTCTGACTAGCTGCTGTTGAGCTCCATCTTCAAATGGATAAGGTTACGTAATATTCATATTATTTGTTTTTGAAAAACAAAAGGAACAGACCAATGCCAAATTTCTTGTTTTATCAAGAGGTTTGGTATTGCTCTGTTTTTAGTAAATTTGACTATACTATCTTATATCTTTATAAGAGAAACTATATATTATTATAGTTATAGATATTTTTTTCTATTTTGATTCTAGGAAAGCAGGAAACATAAAATGTATTGGTGGATGTTAGGAATTTGAATCTTTCGAATCCCTAAACGGGATTGGTTGTAGGGGCGGATGTAGCCAAGTGGATCAAGGCAGTGGATTGTGAATCCCCCATGCGCGAAGACATTTCGTCCACTCGCCCCGAGGGCAGATGTATCCAAATGTACCAAGGGTTTAAGGCTTTTCCCCCCAACACGAAAAGAGCGCCTCTAGTTAGGGGGGGGGGGAAATACTTCCTCTGACTAGCTGCTGTTGAGCTCCATCTTCAAATGGATAAGGTTACGTAATATTCATATTATTTGTTTTTGAAAAACAAAAGGAACAGACCAATGCCAAATTTCTTGTTTTATCAAGAGGTTTGGTATTGCTCTGTTTTTAGTAAATTTGACTATACTATCTTATATCTTTATAAGAGAAACTATATATTATTATAGTTATAGATATTTTTTTCTATTTTGATTCTAGGAAAGCAGGAAACATAAAATGTATTGGTGGATGTTAGGAATTTGAATCTTTCGAATCCCTAAACGGGATTGGTTGTAGGGGCGGATGTAGCCAAGTGGATCAAGGCAGTGGATTGTGAATCCCCCATGCGCGAAGACATTTCGTCCACTCGCCCCGAGGGCAGATGTATCCAAATGTACCAAGGGTTTAAGGCTTTTCCCCCCAACACGAAAAGAGCGCCTCTAGTTAGGGGGGGGGGGAA!.BHPgl~+8EVfq|!.6BM[is #/=HSan|&2?LXcmw(4AMYfr~ -:EQ[ht&2=IVcoz  +8EQ^it   % 1 > I U b m y   ! . : G S _ k u    * 5 A M X d q ~  ! . ; H T a n z  " - 8 E Q ] j v )7AMZgr)5AMZeq}'3@MXdp| $0=IWfs&/:DN[hs %/@JWfr$19DOZju$1=MXdn}#.9DOZev!.BHPgl~+8EVfq|!.6BM[is #/=HSan|&2?LXcmw(4AMYfr~ -:EQ[ht&2=IVcoz  +8EQ^it   % 1 > I U b m y   ! . : G S _ k u    * 5 A M X d q ~  ! . ; H T a n z  " - 8 E Q ] j v )7AMZgr)5AMZeq}'3@MXdp| $0=IWfs&/:DN[hs %/@JWfr$19DOZju$1=MXdn}#.9DOZev (("3':'4-::::.$:+>&>)>>//>>>>>/> />>1,>;;3, +;;>>>>>>>>>>>>>>>;>;>6>>>>>;>>>>>>>>>>+6;;>>;>;>>>>>>>>>>>;>>>>>>>>>>>;;>>>>>>>>>>;;>>>>>>>>>;>>>>>>>>>>>>>>>>>>;>>>>>6>;>>>>>;>>>>>>;>>>>>66>>>>>>>>>>>>>>>>>>>>>>>>;;>>;>>>>>;;>;>>>>>>>>>>;;>>>>66>6>>>>>>>>>>>>3>>;>>>>;>;>>>;;;;>;>>>;;;>>;>>1.;>>;;>>$>;>>>;>>>;>>>;>>>>;;>>8>>>  &        %         #        #  (("3':'4-::::.$:+>&>)>>//>>>>>/> />>1,>;;3, +;;>>>>>>>>>>>>>>>;>;>6>>>>>;>>>>>>>>>>+6;;>>;>;>>>>>>>>>>>;>>>>>>>>>>>;;>>>>>>>>>>;;>>>>>>>>>;>>>>>>>>>>>>>>>>>>;>>>>>6>;>>>>>;>>>>>>;>>>>>66>>>>>>>>>>>>>>>>>>>>>>>>;;>>;>>>>>;;>;>>>>>>>>>>;;>>>>66>6>>>>>>>>>>>>3>>;>>>>;>;>>>;;;;>;>>>;;;>>;>>1.;>>;;>>$>;>>>;>>>;>>>;>>>>;;>>8>>>  &        %         #        # 4.AQ.82L M$  P!^TNSG> c@G,501?PU]KND.$$@FGdT`6 G6>&L1#;<I@(@HMH70a?9U5AB4 *C>F17 56R?`AC`#NI'7% GKTRM4QM22/V 9Q QK4MI4(* Q_)O2C (@0c<L%$c4E)I1L35`1Z)\XR"1&#7(C]84OD\7;*V-X& +\B'3 P:'#0cdEP8:`?'E $*>&UMW88NB .@U%OU-_<TK6S,MND'#Y, ]+V1b8ZV!2(OP4'O`+>)A< 0+")Y1 <V%'PE=^-E#,/;c<2?a78@<=@GLSZO %JA &OZ?cU8>2&WCb2X8\O2 2Z$;/#82@O] XGF 4;,/ .2b%6&/@$3 b_\XUQQ  2u-8uPND9U/J|P qgjLkCZ]U`e/UJ?,n"itVT;g8r.~qY-n}wmfv5*6hRv.tbw1*hX L5J,s!VDN+]B7e6i*"#gwDu{<{)mm7VMF!62\0[)Qz^Wat"!lc y.@:d;j~&s] 4*F z2+$QM1Vh{Lor. z93?`R/kVPVa#{=2 ;aBtxZ1%8&Jf?mr/T~^rT<{i[ Hhg0 Tqr Bndb[SASdYgJ@fSX8;=?:BE61%@.('&   .             x*  x1@Knu((((((((*% %%%#0%%%%BBB9*%% BBBBBBBBBBB+nGyp QG)py'evaEdLH s4w"P8;n{XXp@ dH 9^_#[q?P#*Qvp8q1#L&3x >UCH |&E(8\1(XWo[{aJ_pVe,,'0/ cp/pVMYd,qo;@\)3NEl )1wR(EV8WCq&^6+{LdpSbl) |'A5K:w8<ob( F"&AF,? vEo.##I-:w!;0+2*08 75)#:0C0%'()E+H'"Ggy@:G7/#6)&%!" )'5*6(12&&%M&-#0&-,U(,'/-&W* 2?;3I=Z?%9%4 ;!.5 +%@(ET'4K;<#CS*@?6`,8)D)>@1:; 74T>.Q#U>0>bjE(JZ`6+9T){>?-:L$e(!!.F&L9'%*C'[2?<M5#9(8H#J6'H6H6DSC5qyjyA..TG=3:bubW@j"=N;cLLVA#-|veN5:\R\F5WaJR-gWF2CACAACTTCTAACAATATGCGGTGGAGCGCTACATAAACGGATTTAGGATGCCACGGGATACACCTCCCAACCCCCAAAGGGGCACTTTAAGGATTCAAGCCCTACTTGAATCACCCCAGAAGGAGAAGCCCTGCACCTCACAAAAAATAGGTTCCCTGAACACGATATAAGATAGCAAGGTCAGATTTACCACACACGGAGCCAACCCCACCCCCCGGACAACCCAGAGAATCGCCGGTTGGCCGGTTCCTTTTGTGTCCCAAAACAAAATAATTAAATTTAACCCACCCATATCCTTTTCAGAATTGACCTTCACAACCACCTCGTTCTATAGGGTGATTTTAACCACACCTTAAAAGACAAAAAAGGAGGCCAATCCTACACTCAAATCCCAAACATAAATGTTTCTCTGTGACCACCGGGTTTTTGAAGTAATGGGGACAAGCGGTGCAAGTCACGTCGGTTTCCTTACAAGTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-04-13 04:26:52 -04:00AEPt3AEPt3APFN CAPXV2APrN APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTSTBufT"DCTIDܫCTNMܱCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DDATA"DRDATA"DDATA"DDATA )JRLDATA )JR7DATA )JRtDATA )JRDCHTDSamDySN µDye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT JEVNT VEVNTbEVNTuFWO_GATCGTyp!!”HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~S>LIMS!!LNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOIS/OfScXP1AMByP1RL>P1WDF3P2AMMP2BAQaP2RLIPBAS1PBAS/PCON<PCON;PDMF,PDMF/PLOC7KPLOC3PSZE`PTYPPXLBRGNmܣRMXV1RMdN ԍRMdV1RMdXԖRPrNqRPrV1RUND  RUND  RUND  RUND  RUNT 8RUNT RUNT RUNT ,Rate ˆRunN""܁S/N%/SCAN3SMED SMLtSMPLSPACAd$SPAC/SPACAd$SVER4.0SVER TSVER,,SatdX^Scal@Scan3TUBED3Tmpr<UserphARphCH/phDYbigphQLcphTRphTRkThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN CAPXV2APrN APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTSTBufT"DCTIDܫCTNMܱCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DDATA"DRDATA"DDATA"DDATA )JRLDATA )JR7DATA )JRtDATA )JRDCHTDSamDySN µDye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT JEVNT VEVNTbEVNTuFTabc"FVoccFWO_GATCFeat//bGTyp!!”HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~S>LIMS!!LNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOIS/OfScXP1AMByP1RL>P1WDF3P2AMMP2BAQaP2RLIPBAS1PBAS/PCON<PCON;PDMF,PDMF/PLOC7KPLOC3PSZE`PTYPPXLBRGNmܣRMXV1RMdN ԍRMdV1RMdXԖRPrNqRPrV1RUND  RUND  RUND  RUND  RUNT 8RUNT RUNT RUNT ,Rate ˆRunN""܁S/N%/SCAN3SMED SMLtSMPLSPACAd$SPAC/SPACAd$SVER4.0SVER TSVER,,SatdX^Scal@Scan3TUBED3Tmpr<UserphARphCH/phDYbigphQLcphTRphTRITNn%&nAJ9."G>H15KK;x^OSvqy4=(Wz`&t$SUf^3i&6j|z[vRP|ZzrA^iaHtV2}2?Yk6]DZSPW~VlWj+8w*<_swW^$oVv+rrbN<6h8b`[.b;$,PCH-p9b6mtTUk1T:29"5-z]T1S6RA0W7yLlo/&U{uclQ[vL}W$P1ObYn#pwW(YJ_93dy5*=gb_nwkI6dNNwM@B8{A)P|p$S!T,lwd-l#fr2i:}WL~?O./bE:#&PV)ob&6O`Z?5t}"^wuCt(3ksKp/(ZU&benN#;98wXu0oPnBT16Y-e+&54bn%&|CO1h,9n5N`!1;ULWyS#|Y;;y+Y3y'EV}sRCkSh!0i9>Yn2Pajeo4]tRi^as>0cPX*F8Bir!3EY$9eKoQD5pYHT"Hf.Q*s`AtI_h!"9;32-;(284;U59b/D?*{|D$haygK >xP `v?{}I8w$YWmOyl}$rv/8NpFQlUAT/n@Z-I+TwM"f^@/(-[mMOMh7&05OPh~wA[h@tFt(#jyO~6X~3"F*+#dgU[_5:W^$OufcGHO5QfOl>o ZbCd]'GE#!#hmmfc4.=+I}S$o:/bEmKB6KP8)s5AVihx_7&OMb=KuRZ+kM7yW@"SD=y!.qK|-o*u0#|UUQ1V? MlYXjlnK[Yl`A #pJE,#VuU4zr)v4+A~#/zM{ZUnVIWz7K>0N}5!){mnjH[v]Eb,8HW>ycPJ~FT,{1:Kn6?_s2n`457^^sIdwz@{&]t[X'(A(f'PJA}*4d#kE_GY@ZWeoPTF}cyR6A;{IF}o-jAS_7ea_';_/Ft(#jvlj,DmZnyI;!H|x]0oPGlOG!>RE.$2<2oYWA8aM|`9}LK4mQ;R#:"`TDhcL(+gbk1^DR!_TcM#yKA[,5S[l'ny#RqAEPt3AEPt3APFN CAPXV2APrN APrV1APrX}}ARTNASPFASPtASPtAUDTllrB1PtB1PtBCTSTBufT"DCTIDܫCTNMܱCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DDATA"DRDATA"DDATA"DDATA )JRLDATA )JR7DATA )JRtDATA )JRDCHTDSamDySN µDye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT JEVNT VEVNTbEVNTuFTabc"FVoccFWO_GATCFeat//bGTyp!!”HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~S>LIMS!!LNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOIS/OfScXP1AMByP1RL>P1WDF3P2AMMP2BAQaP2RLIPBAS1PBAS/PCON<PCON;PDMF,PDMF/PLOC7KPLOC3PSZE`PTYPPXLBRGNmܣRMXV1RMdN ԍRMdV1RMdXԖRPrNqRPrV1RUND  RUND  RUND  RUND  RUNT 8RUNT RUNT RUNT ,Rate ˆRunN""܁S/N%/SCAN3SMED SMLtSMPLSPACAd$SPAC/SPACAd$SVER4.0SVER TSVER,,SatdX^Scal@Scan3TUBED3Tmpr<UserphARphCH/phDYbigphQLcphTRphTR