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***>3>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR9~}yxwtrpoPPPGHHefe}}}  '((000888AAAHHHPPPWWW^^^eeellkrrqyxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 83484d6c25964042b2bce1790e94c1bdSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-04-12_23-54_19833130Seq1013310133_Lehman_K1359_psbajmadera96-Well####""#"##"#####"#"####"""##########"##"###############"###############"#######"####"################"###################################################"####"##"########"##################################"##"#############################"##"#################"##""###############"##########"############""########################################"#####"###########"#########"################################"#########"#########################################""#######"###################################"#####"######"###########################"######################################################"#######################"######"###########################"#####"############"#######################"#########"##"########"######################################"###"##############"#"################################"#############"##############################""############"############"##################################"#############"###############"############"#""###########################################"##"##################################"#"####K1359_G02_psbaKB_3730_POP7_BDTv3.mobSeqPop7z 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. 9 E R ^ j v    * 5 B N Y e q |  $1<HT`kw)5ALXdq| &2?KWdq} &1=HT`ly !-9CL[ht  -(#2 555  !0):::1>/>>>1>>>66>>>>88/>>>>>>)#"";;;;;;>>>>>>>>>>>>>>>>>>>>>811>;>>>;;.#6;3.>>>>>>>>>;>>>>;;886>>>66>;>;;;0.;>;;>>;>>>>>>>6>;;>>>>>>>>>>>>;;;;32"6>>>>>;>>;>>;>6;>>;>;>;;>;;>>;;;;>;8;;;;;>;;332.231>>88;.283;3331+1;>;>>>;;6;38.88;>."+++;>;8>;>;>;;;;>>>;>>68.13'33832+;;3;;;38802.!;;8;33333;8;;3++3;;;;8881;;>;338>2;8;>8#,33+38 &83//8>388888/3&,333%%   -(#2 555  !0):::1>/>>>1>>>66>>>>88/>>>>>>)#"";;;;;;>>>>>>>>>>>>>>>>>>>>>811>;>>>;;.#6;3.>>>>>>>>>;>>>>;;886>>>66>;>;;;0.;>;;>>;>>>>>>>6>;;>>>>>>>>>>>>;;;;32"6>>>>>;>>;>>;>6;>>;>;>;;>;;>>;;;;>;8;;;;;>;;332.231>>88;.283;3331+1;>;>>>;;6;38.88;>."+++;>;8>;>;>;;;;>>>;>>68.13'33832+;;3;;;38802.!;;8;33333;8;;3++3;;;;8881;;>;338>2;8;>8#,33+38 &83//8>388888/3&,333%% I JLc"4 B5$() Z2daA)^5KC-?&1:)!EZM,#'/R XE, %N_KI )]49d);I.> cMSK-, `OXWUD5(U&dSDM -+6VLT$!FN.U6U/;H-#"3&J=>R Ea$@G"L+X#b6N/'#J62 2M9 -VC1RON8TE%(ICDR.\3)U-X1F?\G3/!K3G=T< F`= O!/?K\S,X9%+;dE @]:[%,5CF303Q6#I+D$OD!GRX<L/ [CX)$J8&M<'*]S]0!SD:=X-FL;<W K)X0a6BX36;-<aIO.H0A_%+9G8( \_JJP=2 U9<b1V2T$vhr R\Nsp7c\?ttr#nP#]hY ?4v]}[xtI 'tO>huh" i$E(RJdb.G67&j16qY>Lv3azWg}r i:x r(wp]wU ]!|k/ 5!7gs'} Sg{>y9WASbIa}FD b)B N("3x4$Im;3qpH\O#RKx {Nn:KE=<WF#C^x bSiPX H[h'h7s |qv[mM,[jSgTuVV+99:21/3568/<-2"("/0:5#             2          #.%-W>pp+ 1k+`7{bR.na%1M",%h e:&P#<~L;i4p%0E(H8,):pH3M~WhRh L8Lyu7#8v&$Q(_-mE~`2L p2Lb.*xN4RdgD$A'UK" kQCQuF\D x s4cNph6z_A:s[O(X+$V<*)#9&#%#02?<`3 /(6S/?P2o1  ;:<.%.8'D @i9@8*-v~/'6K=+7CF4% 1!&'=12,=.E-D^-TRL W AMTi^Y  X Z< 62($'RB]iA t f]hTXBWgEPKJd8RJJ}fY^_r{BKN1_cMeg['Choei nluFT:|~<P3 ^L#CUDxRcnVJzhbgj@4QK{{{hk-fW2_dO|?#alslKU_|swda\0fp#!tH]u& g2CAACAATTCTAACACTATGTGATATAGATCTACCACTTATATATTTGAACTAGTCATTGCATATTTGAAAAACCCCCACATAGCGCTCCCATTTACGATTTTGACCCTCCAAGTCCCATTTTATAAAACCCCTAAGAAATAAAAAAGAATTTCAGTCGTGTCCCTGCCCTACCCCCCTAACTTTAATGTGCCGCCAGGGCTCTTAGGCGGGCCTCCAATTGAAAAGAAAAAATAGGAGGCGTAAGCCCTCTCGATTGGACAGGAGTGAATTCATTGCTCCTCTTTATTTTCTAATCACAAACCTCCTATACCCTGGGACCAAGTCTTACCCCCCTGTTGGAGCTTCGATGGCAGCTACGTCTAGAGGGAAGTTATTGACAGTTCATTCATGCAGAACGCACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-04-13 01:24:50 -04:00AEPt3AEPt3APFN APXV2APrN APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTSEBufT"DܙCTIDkCTNMqCTOw܉CTTL ”CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DDATA"DDATA"DVDATA"DDATA (+PV DATA (+PV5bDATA (+PVDATA (+PVDCHTDSamDySN uDye#DyeN€DyeN…DyeNŠDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT  EVNT EVNT"EVNT5FWO_GATCGTyp!!THCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~DbLIMS!!LNTD2LsrPaMCHNMODF ¾MODL3730NAVGNLNE`NOIS&lOfSc8P1AM"6IP1RL"3'P1WD"9kP2AM"?P2BABP2RL"<PBAS(0PBAS&PCON1PCON0PDMFPDMF&PLOC",PLOC")PSZE`PTYPܑPXLBRGNmcRMXV1RMdN MRMdV1RMdXVRPrN1RPrV1RUND  RUND  RUND  RUND  RUNT 6/RUNT )RUNT  RUNT  Rate HRunN""AS/N%&dSCAN3SMED SMLtSMPLSPACAiwiSPAC&|SPACAiwiSVER4.0SVER ESVER,,Satd8^Scal@Scan3TUBEG2Tmpr<UserphARphCH&phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTSEBufT"DܙCTIDkCTNMqCTOw܉CTTL ”CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DDATA"DDATA"DVDATA"DDATA (+PV DATA (+PV5bDATA (+PVDATA (+PVDCHTDSamDySN uDye#DyeN€DyeN…DyeNŠDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT  EVNT EVNT"EVNT5FTabTFFVocT5FWO_GATCFeat//TGTyp!!THCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~DbLIMS!!LNTD2LsrPaMCHNMODF ¾MODL3730NAVGNLNE`NOIS&lOfSc8P1AM"6IP1RL"3'P1WD"9kP2AM"?P2BABP2RL"<PBAS(0PBAS&PCON1PCON0PDMFPDMF&PLOC",PLOC")PSZE`PTYPܑPXLBRGNmcRMXV1RMdN MRMdV1RMdXVRPrN1RPrV1RUND  RUND  RUND  RUND  RUNT 6/RUNT )RUNT  RUNT  Rate HRunN""AS/N%&dSCAN3SMED SMLtSMPLSPACAiwiSPAC&|SPACAiwiSVER4.0SVER ESVER,,Satd8^Scal@Scan3TUBEG2Tmpr<UserphARphCH&phDYbigphQLcphTRphTRS1P7!3AQi4'.QmjMy J1i2boR"^OH1sbu!M4M\mk*+_Wr9|>u2KTTkw^8-l278;;9Vge!Yz]%MJeVId4|Cn=aib*y%`;5m;*/P9m4%2pQWi?oFciW 45=)ySQbir8r|:7'I3#wor({Y]?\/t]66{qT[sEAhiW?WUBs*u hb7+#?Q{6Jn`|T6{?v2FKeS5~i2o8xOrz_lK63<%Ri24WXXp}@c[#m*Uc WUgK:=[:"yzv+rrbNn%Q8^L."gE{}Vm)HNtXehapc?_)54n,\(nqBz_^_<~H)&6AEMCBt9}j~:Hz4W9W3|7nM6!"/D?*128)TK72K`rx./vzFl,"SZ*]ilWAz6@f9-K!'[/ C,Z:X=<;`O|g+Vsp@OJ-12Mm8joIiD60mPa6(U.*)./"Dlyj,K]yDWa%2gP')3g;Z6ObeIXz7_lnqR03AGGK.97V=y`6@vdeW\jhAZG|JWm;2FVOR(_Y~8b`KJk/&UPh_^_,AG)!OiK(/(WWh[8'[%k Vl+?Ou\![H~?p MD8zEXTMk|k.j H5Xv&~^\mcJT&1o?e3{0+VNwBx6Ks;6R&8yT,I}_7~j{nF?/aOw2gpO0,cW`GCYHs@dUa0u:W^$)P? sI}}1%j-]Tsz^-d+O'i~88u h`k@_[O~}<"&eG1<@O(LxCmqL6&pg;gjmMUwch[}k@_ajF= =&e=.7I 9'k*|QK:}$OEH3SP')3g;Z6ObG`<(:&I;u4,ZE_GY@ZWeoa$sp@OJ-omMOx8yT,I~<'",s@3[{R9Ya)@Xp}"aFV;LX4KU>?+Nhsr5%NHP>$z 4+$8N[!o_M,? 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