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%   !+   #    *(+5 $% %)- )4*%/8>&344%J'1,"263/-.*;;.%*2%F7@/R74<5957GC5LIXdcJPM>>9C)'/3@)5E89<6,,5C?<4*'>B?A@51EH9QD"?9@>J<+,"$E217@3+0&$,.-#,#.A#).5+44,*:M3#3A@<;@@8@01+0+AG7.NBAC-3%$#0E,/%'7.#$(,5<<H[1>5S56<69A,%)0,)2.-)-"$!3/.,30#      !$          #           "                                   #         % /) "$ &$3.# #&'!( & a",,III^^^{{{&&&999DDDXXXaakvv      ***33>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRp~|zyuurpomkigfd3OOOGGGddd{{|   $$$---544===DDJLLRSSZZZaa`hhgnnmuttzyy<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 2928c4e660a644be8d3103bb9010798eSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-04-11_19-58_19723130Seq1012210122_Lehman_K1356_psbajmadera96-WellK1356_E07_psbaKB_3730_POP7_BDTv3.mobSeqPop7z,?IR]m(7DMU[ais~xncXK?2% yqlhfdcbdjs~yncYSQQRQMIECELVbny#7J[gmkcUC.tM&o\K?5.(#  !""""$&),/12456788888;@HR`q <q WzS*pe`_bjs~ ,X+[`s`eQFBCGJMOT_w!|]hlt U!&U6# $*4D\~,BQM -iL>:=DN[kU1}$[NY?0(%$#! !%+16:;960)! 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'^[y+C   C9}3D      8vd~?V%  0`12cACjRPk6   8i'"V)*QyI#         HO[a\a(\ +Eisfdo,$tG'  5b)4&e<   -_6pg+f=$"#"     k AIA%-A_cA".KB.bcpKB_3730_POP7_BDTv3.mobtermAGGGTCAGTCTGATCTAGCTGCTGTTGAGCTCCAACAAATGGATAAGACTTGGTCTTAGTGTATAGGAGTTTTTGAAAATATAATATTAATTAAGGAGCAATAAACTCCCTCTTGTTCTATCAAGAGGGGTTATTGCTCCTTAATTTTCTTTTGTTTTGAATTATTAATATTTTTGACTAATATTATTATTTTAAAGATTTTTTATTATTTAATAATAATATCTAAGAAATATTCAAATAAAGTAAAAAGGAATTATAGTAGAGGGGCGGATGTAGCCAAGTGGATCAAGGCAGTGGATTGTGAATCCACCATGCGCGAGAGGATGTGGCATGAACATCATGTTCTTTTGAATGTTGCCCCACCCAGCCACAAGGTTTGATTCCCACCGCCAAACTAAAAAGGGTCAGTCTGATCTAGCTGCTGTTGAGCTCCAACAAATGGATAAGACTTGGTCTTAGTGTATAGGAGTTTTTGAAAATATAATATTAATTAAGGAGCAATAAACTCCCTCTTGTTCTATCAAGAGGGGTTATTGCTCCTTAATTTTCTTTTGTTTTGAATTATTAATATTTTTGACTAATATTATTATTTTAAAGATTTTTTATTATTTAATAATAATATCTAAGAAATATTCAAATAAAGTAAAAAGGAATTATAGTAGAGGGGCGGATGTAGCCAAGTGGATCAAGGCAGTGGATTGTGAATCCACCATGCGCGAGAGGATGTGGCATGAACATCATGTTCTTTTGAATGTTGCCCCACCCAGCCACAAGGTTTGATTCCCACCGCCAAACTAAAA '4CIU^ex*7DVfp}  +9FO[iw $2@KVbnz)5ALYdp~ #/<HUbny*6AKXfq(4@LXdo|!.:GT^ku    ) 5 B N Y e q }   $ 0 < H T ` k x    * 6 A M Y f q }  # / ; G T ` l y    + 8 D P ] g s  &3?JT`kt~ !-8FS^gr'5BKVbpz-7BMXdn}#/9FO\fr '4CIU^ex*7DVfp}  +9FO[iw $2@KVbnz)5ALYdp~ #/<HUbny*6AKXfq(4@LXdo|!.:GT^ku    ) 5 B N Y e q }   $ 0 < H T ` k x    * 6 A M Y f q }  # / ; G T ` l y    + 8 D P ] g s  &3?JT`kt~ !-8FS^gr'5BKVbpz-7BMXdn}#/9FO\fr   #  #(5%-)::0-4$:116:>>>>>/8+//>>>>>>** 88>>>>>>6>>>>>>>11+>>>>>>>>>>1>>>>>3;+(.+3>;>;>>>>>>>>>;>;;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;;>>>>>>>>>>>;>,&&;;;>>>>>>>>>>>>+>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>/>>>>>>>>>>>>>6>>16>>>>>>>>>>>>/6>>>>>>>>>>>1/>>>>>>>>6+)/>>>&"                        #  #(5%-)::0-4$:116:>>>>>/8+//>>>>>>** 88>>>>>>6>>>>>>>11+>>>>>>>>>>1>>>>>3;+(.+3>;>;>>>>>>>>>;>;;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;;>>>>>>>>>>>;>,&&;;;>>>>>>>>>>>>+>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>/>>>>>>>>>>>>>6>>16>>>>>>>>>>>>/6>>>>>>>>>>>1/>>>>>>>>6+)/>>>&"                     WM(M*D7$+FSZPZ@S")`S # `(/U1]+-MW5B .RA(S]6I]"X[>([M727^<TZ^D@>]A>LdQc*B%$1!Y)HB?DG Sc$-*`#/`E!="[_(2 LQSCUB , *D.Lc:%!&D@bRYH'S6EG5#b]G# 7D#%d6"F;"X'8Lb0a`.$W%&[0A'#1. /FZF"W9-G7=[X-Y%S?8K":WV %N Q"C'2!K$1C(55;BH D**!&$ZK\ /S8OX&ZI?DB.Na_?2/_Z222F[!"_7*_ 5"=TA2c a92H_MD _D1 _JDP__5/._DD __Kb%,;\)6z@+ 6 cNF|-Vj(qdt-EU. u*;{ AOrR .L @BW+e(/>ke|37  3V([]3P=A?hs<e\(QjLP1CT+^ 8)9P4#M]0$9(*9Vrv=58c=[w)%3@zV`y(&u /S.&W^ -#BSdWrl4(128 Q 6V e|K%3<|9'qs r ! =2~0IZ=' Og HJl%4XdzO|bUkuQtihcDFP8gUMRH?A;QBK>>@E) 5LR8?0:#$#'      =!!                         %         1G! +2(& 11111111111111111111111111f111111111111111111111111111111111111111\!bpp~E%pIU8P, %2L]$#zrr w}*df\pgE,Vpm{e8Y4481h8TpEs  @dpapz(3"Sp$JD@[0(Gv1A?"=Z"o_Es5X#+   !0?&;@>B*       ,  "Ipyl/?4  0&% ") """  ! #8H +  ( ""#- ! $ Z0<P!/(:0z`:H?"`"Iy:FEE(!>&g(*@^ i, 1j$U=b*^t[GAAACTCTCTAATCTATATGCGGTGGATCGCTACCACTTACATAGGCCACCTTGTCATTGTGTATCCGTGGGCCCCCTTATAGGATAAGGAGGGAAGAAGTATTTTCTTTCTCACCCACTAAGGAGTAATGGGACCTCGACCTTAAAATAAGAAAAACCGGAAGACGGGGGGACCCGGGTGGGGGGGATAACCCCCCGGGAGGGGGAGACCCCCCGGCTAGATCCGAGTTAGGCTTGTAGGGACCCTGTTATTACGACCAGTGTACCGCATACGCTTAGGACCATAGCTAATGAATCCACCGTGTATGCAACGAGAAAGAGATGCAATAAGGTGTATCTTCCCGTAAAACGGGAGCAATTACAGGGCGAGGGGTTACCAGGATGACTGATTGTTACTCGCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-04-11 21:29:30 -04:00AEPt3AEPt3APFN APXV2APrN wAPrV1APrX}}€ARTNASPFASPtASPtB1PtB1PtBCTS?8BufT&LICTIDCTNM!CTOw9CTTL DCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&L^DATA&LDATA&LDATA&LBDATA '`NDATA '`N3DATA '`NDDATA '`NDCHTDSamDySN %Dye#DyeN0DyeN5DyeN:DyeN?DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE7LAST~~=LIMS!!MLNTD2LsrPaMCHNMODF nMODL3730NAVGNLNE`NOISP1AM /P1RL ,P1WD 2P2AM 8P2BA<P2RL 5PBAS!PBASPCON*PCON)_PDMFPDMFPLOC &?PLOC #PSZE`PTYPAPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND  RUND  RUND  RUND  RUNT :RUNT %RUNT RUNT  Rate RunN""S/N%SCAN3SMED SMLtSMPLSPACAfxtSPACSPACAfxtSVER4.0SVER ?-SVER,,Scal@Scan3TUBEE7Tmpr<UserphARphCHphDYbigphQLcphTRphTRAThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN wAPrV1APrX}}€ARTNASPFASPtASPtB1PtB1PtBCTS?8BufT&LICTIDCTNM!CTOw9CTTL DCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&L^DATA&LDATA&LDATA&LBDATA '`NDATA '`N3DATA '`NDDATA '`NDCHTDSamDySN %Dye#DyeN0DyeN5DyeN:DyeN?DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabMFVocMFWO_GATCFeat//MSGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE7LAST~~=LIMS!!MLNTD2LsrPaMCHNMODF nMODL3730NAVGNLNE`NOISP1AM /P1RL ,P1WD 2P2AM 8P2BA<P2RL 5PBAS!PBASPCON*PCON)_PDMFPDMFPLOC &?PLOC #PSZE`PTYPAPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND  RUND  RUND  RUND  RUNT :RUNT %RUNT RUNT  Rate RunN""S/N%SCAN3SMED SMLtSMPLSPACAfxtSPACSPACAfxtSVER4.0SVER ?-SVER,,Scal@Scan3TUBEE7Tmpr<UserphARphCHphDYbigphQLcphTRphTRk#[Nn$Q2CWllm4y[*M0Jhjag!aczjCt>-8G8A,Fl.XOAGi[UePd7n%&/VmO\rwHB*@Y%H_TQFGa^_#=?o'%x'[vA-\K"L1{!UlyN7:I1DD[ho* xv'o>s1HT/(J8dR9JK[Yle9Su3XwC^8m]Cy4=?6vtfQgsk>H~pL"A~oTq}}89u:CQ{&q1GUW!\`C}B-mq5FaT9Vbr$rv/gg61CNdW)j-[YD8J)T(k"T'oA_AN;)oX6YMI)0g'^+oL.SVX*os7}Z&['Ko;X^`>EOCy KQI5m6``^,Ffn@axsA MG }OPmE$ww*{we* _ZZ^"4/GKTrQ3oS"Y|8by`AVih{3r< wvr]LaiY2]NDzU4obir8rP p'Ic|4`A(^:go8m+adJs _ZZ^USn&vrOU[.(3`C/fshkr+E`_W>WM@A<}1X*#PE|GO 9 MV%S!CxAe]rbZ]"Po1Cd}F<_`VWYH|O[nY\Kwoh^*p%!++(aQQ*G]mysG(B]mzQLAEPt3AEPt3APFN APXV2APrN wAPrV1APrX}}€ARTNASPFASPtASPtAUDTll[B1PtB1PtBCTS?8BufT&LICTIDCTNM!CTOw9CTTL DCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&L^DATA&LDATA&LDATA&LBDATA '`NDATA '`N3DATA '`NDDATA '`NDCHTDSamDySN %Dye#DyeN0DyeN5DyeN:DyeN?DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabMFVocMFWO_GATCFeat//MSGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE7LAST~~=LIMS!!MLNTD2LsrPaMCHNMODF nMODL3730NAVGNLNE`NOISP1AM /P1RL ,P1WD 2P2AM 8P2BA<P2RL 5PBAS!PBASPCON*PCON)_PDMFPDMFPLOC &?PLOC #PSZE`PTYPAPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND  RUND  RUND  RUND  RUNT :RUNT %RUNT RUNT  Rate RunN""S/N%SCAN3SMED SMLtSMPLSPACAfxtSPACSPACAfxtSVER4.0SVER ?-SVER,,Scal@Scan3TUBEE7Tmpr<UserphARphCHphDYbigphQLcphTRphTR