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"+$ $$5!?7<)6H7B;'&9'X:FLF5@8,8D7)&KH4'2<;JGM?A>>C&(<>%G?B?BJE=C*8B?C46'%+=6)* #        !"#$2345!"#$2345dx,,,III^^^{{{&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR7y}|{ywurrpnlihgeOOOCGGddd{{{   #%%---555=>=EEELMLTTS[[[baahihnnnuuu{zz<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 171926807dd54156b4e2b9b6b3da5912Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-04-11_15-26_19693130Seq1011910119_Lehman_K1355_psbajmadera96-WellK1355_F11_psbaKB_3730_POP7_BDTv3.mobSeqPop7z###$%&(*,./0//.--.13689:::::;<>????@@ABAA?>=>@DINSUTOH@82.+*)(&#! 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3VEab:+PVL :DEH2XQ&$#H5.6&SF3\./\B2>9UbZ CH[ZY<K "^BZ56A /XD["Lc8VO4K-EELUELS?0(4E(.]D (0=*?L;MD0<8X;a+, .8L %0I%YDG:a2HO_7Fcd<6":^;aC(b4ab0 #=W 'A[7M$'Z5T/O *I`M 2( w@o)6D"TtdVV} k!#xY`ZRJ9-3bGAt*]F-=^ l8!\pYI~>JHL'A1d,kmizjX-R0 ?^$JL 2PHSSvN"~o=}\"Hz2+ZgYgBI|#Z;!'5]h0k79rtze!+3>Lap;itw=\lzcnYH9_vx#wgb )Gl)JF YqcKBVrDG#h*H6 In GL5I*O hGXuk(}]0CuR *`9N"u\7^Z HF :Q8&Fw{fifbf@@oK@A<MAL B>/J1=4%,'353  ''             Z'J#1KvW[ 9''''''' '0'''''''''''''''Knupdp\,{%,dlNNp8 8pScdDdr+|Hr,Ord*Ex|%qQu/8:l8k{"p'5nbpB'i S,J)kd@T`d+k8DkVp7sddqKgSS 0Q40zld>&dosKD5|@ddhYddq0p8a$,41yr_Lc{zzq,8PHU]?6Ad,lGY36NY.cs2pwjR0D.)5Va -/, & 7-*07$ "An%(" `qJ!!!'>$!     *Fa#!$"!  ! .          )! $              /   # %%prQ:\pM'!1k<<{sm8>DuLf!"Wa&kB-_\k,VGi}Q|)]C@yy:"|Z #`z@v|4ss 5m"c lN#ACTACTTCTAATAATAGGCGATAGAGCGCTACCACGTATATATGAGACCTTGTCAGAGTGTAAGTAGTGGCCCCCCGTAGAGTAACACGGGCAGAACTATTTGCCCTCGAGAGCCCACCAGGGAGAAAAGGGCGCTTCATTAAAATCAAAACTAACAAGAAGAAGTGAGAAGGGCAGTGCACACTCCGAGTCCCCTCCTCACTACAAGGGAGGAGGAGGCAGGGGCTGGACTAGAATCAGTATCAGAACTGGAGAACTGAGAAAGAATAAGACTAGATCGCTATAGGCGGGAGGATCCATATGTAAGATAGACAAGCAGAAATATACGAGTAGATGGACGGAGTAGAGCGCCCCTAAAACAAAAAAGGACCTGTAGTGACACCAAAAGCGTTAAAAGCGCGATCAGGAACTTTTGTGCGTTATTTTGTTTATTATACGTCTCGTATGAACCCCAAGCATAATGTCTAATAAGAGGACCATAATTATAACCAGATBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-04-11 16:57:12 -04:00AEPt3AEPt3APFN APXV2APrN APrV1APrX}}˜ARTNASPFASPtASPtB1Pt B1Pt BCTS=BufT!BaCTID3CTNM9CTOwQCTTL \CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!BDATA!BDATA!B"DATA!BdDATA &TLDATA &TL1zDATA &TL~"DATA &TLDCHTDSamDySN =Dye#DyeNHDyeNMDyeNRDyeNWDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEULAST~~Id`Po<_+MWQ}VX*oE}`Z&oRLV;);-ags5)9mW'>YP~jhM^,%bkb |{cokB}qHqHStXK#XTE-=[c}Sn2M5FiK&=RSbXW,62DmOYX/D?**s$[-p+D42F7f6QuA!9@1%ilJG82G [7P^E$62:gtpMu9ifB;YD<&s6|MR_le/M#yKAI8rBAr_s_bed{a9_ajF=W.X}=_;!;`sEYWC.%ouO{9X6!7&o.jaxJK[Yl)d762H#A#Q9U,7Yv^TTH455-z]G3o6RlSR#yK9YtIRPV]O_,A8 z9X=5>2jP@*F8a?G>Sx+C!K}Z'BNpbX\"B=8djF3u2yl:[>zw#NRd)B*OqPk&OE6OLxPo%?E7\lzqdY(xs/b@j/.3]Hq<6(m=':Z^u4ZP}Gs,"T'oJ-omMOx+6T,IL|2D5^.4*6AHqW`A>nluZogjHbinOY5_}4]'>'s1HTf{fzja:3AG}X`=/r.ZNum#0kc "7:I12gbXW,62Q$OEHbX\"B[M@{`29w/>r|ESRDujXTE-yh;}S$oa$N;172e,qz7|!f|bEdG[~{?26ttRHSK%}jx7Z]et|?%9'o *&AEPt3AEPt3APFN APXV2APrN APrV1APrX}}˜ARTNASPFASPtASPtAUDTll\B1Pt B1Pt BCTS=BufT!BaCTID3CTNM9CTOwQCTTL \CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!BDATA!BDATA!B"DATA!BdDATA &TLDATA &TL1zDATA &TL~"DATA &TLDCHTDSamDySN =Dye#DyeNHDyeNMDyeNRDyeNWDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabL;FVocL*FWO_GATCFeat//KGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEULAST~~