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" #  #                             ) )   ,'              !  +*+& #'!'+(%( '  !%           # *$                  !       +*            "   + -& !(" !!*    % ($                                           &$&  .""!'*").(% '31# ''-0)-*3:)-)",+('!'+ $$"" 1%,$  #%                                         j                                                                     dx,,,III^^^{{{&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR7y}|{ywurrpnlihgeOOOCGGddd{{{   #%%---555=>=EEELMLTTS[[[baahihnnnuuu{zz<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: b312f915b78c4e079ecad6e001eb0972Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-04-11_15-26_19693130Seq1011910119_Lehman_K1355_psbajmadera96-WellK1355_E06_psbaKB_3730_POP7_BDTv3.mobSeqPop7z#$&(+/221/-,,-.03579:;;:98889:;;;:865443320.,*(&$"  !%&'),2;HVgx,Gc}|fL/yeTF;3.+('&%%$"   !#$&(*-049AL[o&JljK+ gSE;510/148=CHMUat[Nv),G,llSF@??@ABEKUf Sh@7SK w $V\C624:AHNTY]aeks,ul"72(ehK9.)*-3<FTfA[f,NgJ6*"     7wlxpOQ_%*d)m0[;)  #&),./.,($ !$&((('%""5Vl >TI#R/ $())%    /}uVpz%2      7jP2QC$l{pUbV+0SA     ,i2ct5dJ /bN=T%     9u' 8}=  FTMG %W}D #&&#         ~24g'F             3u(        hz= 1N?UF]` !j{&'A2>               "xY$ ZQ 7c ~ H  X)(P       ZOjvA0WTOd/Zv4:     *5I^   ~$-,#~      +3iGwJ         5q EbV'M  $`k9; 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"s8{=D          * \y,7    ;tL6'H+=ax"   HB{#~d   M;H?+C     RG[L/B          9=?v3=']b.B: 7ZP] d^e4hdU7z'  4<zj  S=0bJ]@_^=AwQU;J D0A3l T#j_aI w!V> <7iVk3Y/* ?Zp{ qN hrYu+ !''#   *}[j+}(&@G_ (l5Ex/ CpT  I-0rlh 8Ws*] XrE*&$0m_ ZHmfI4*w-Vm&   I}S     727B    D$[S9    'rnTuH;,0X U6     Dm$8W%uV4,fUtd$0 [S > .m$vv#m. ,q@Y< /t?W> 3|SuF )nB ==|L)G 7!P_'1yh Rf)-rp.2~x)L,mF    <Dwe()nEnt-4!_w,  T'4_   <jCn)![jD?KFFL  Wsv\!     7^mE   YsYI     1zL^>    ,kq9@  A@q q3   5]z3$i0nU"#Z1X 6z-xjo1  3w7H@  "V:irTz=  .hMx|Wv9 !TJMY$ 1h1OI"Y(   -gO|_~?&[LI]) JAfo3 A+u}7I @{ C_W.`,   D*pen?@q1eU -^@E(k5  #`yY      3U  V0      "$&(*+++,,-./0123455677777777776655433222233320.,*(&%$##"!  #.A]-<FLPSVXYWRJ@4' ;f F Q*m9[vh@ It2N  i; _;}zvrommptz"Mz(OrnDv@ |iZOF?964344555678::::99:;<>?AEKTbsQ2``/x<tbULGCAABFNYkB1-qg_PuZI>85310.----./001000000.,)%  &AmW.8_cCEqdLDK`_.5e|ypmx0''iUKGECA@AGWvK0IcP<J_<( !"#%&&'&%$"      &dGvRtXi? #&)))'%"  8tP,[y:       Pc[M  #+28<?ADINU[aejnsx~~zvrmg`YRLGB=83-'                 ^_xs^c      ! 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            @@;Q'$j1GT^g=JK=YX|p7|/S:CZ*Fc>P~ ,%NkgpA+ \u Y4A@pwC<pbtUnRU{9KE0?.&HwR)LjhZFF Wt1gkgv2 n>GC)1d[G'W\j~]S8 %HTb3d z"d\3[whnl|I83O'0)5 ,4;#(6sI  fJxW`  ]E      6   - ?'    )    W .&!#   #,   (    ##" !      6 # '  *'g:I[I_7CATATCTAAACACATATGTGGTGGAGAGCTACCACCTAAATAGGAGACCTGCTCATTGTATATAACAGGTTACCCTATAGAGGGGTTACACTATACCGACGGGCGAGGAGGGACCCACCACCTGGTCACAAGAACGGAGAAGTGATTGCCCGCCGCAAACGGGGAGGAAAAGTTAATAGTACGAGACAGCAGGAGTCTGTTATCCGCCCTACTGTAAGAAGAGTGCGTACCCGAGGAGCGGGAATGGCCCATGCGGCAACGACGGCTGGTCCGTTATTCCCGTAGGGGTCGAGTATCTAAATTAGGGTCTACTCATATAAGAGACCCGCAGAAATAGTCGAGTTGATGGACGGAGTAGAAAGCGTATAATACGAGAGGGGCCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-04-11 16:56:34 -04:00AEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPtASPtB1PtB1PtBCTSFBufT!B!CTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B^DATA!BDATA!BDATA!B$DATA (jPDATA (jP5fDATA (jP:DATA (jPDCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE(LAST~~DLIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOIS'P1AM7P1RL4P1WD:P2AM@ P2BAC P2RL=PBAS)PBAS(PCON2PCON1PDMFrPDMF(PLOC.PLOC+PSZE`PTYPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrN۹RPrV1RUND  RUND  RUND  RUND  RUNT RUNT 75RUNT 0,RUNT 7Rate RunN""S/N%'SCAN3SMED SMLtSMPLcSPACAfSPAC'SPACAfSVER4.0SVER FSVER,,fScal@Scan3TUBEE6Tmpr<UserphARphCH(phDYbigphQLcphTRphTR pThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPtASPtB1PtB1PtBCTSFBufT!B!CTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B^DATA!BDATA!BDATA!B$DATA (jPDATA (jP5fDATA (jP:DATA (jPDCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabTnFVocT]FWO_GATCFeat//T.GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE(LAST~~DLIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOIS'P1AM7P1RL4P1WD:P2AM@ P2BAC P2RL=PBAS)PBAS(PCON2PCON1PDMFrPDMF(PLOC.PLOC+PSZE`PTYPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrN۹RPrV1RUND  RUND  RUND  RUND  RUNT RUNT 75RUNT 0,RUNT 7Rate RunN""S/N%'SCAN3SMED SMLtSMPLcSPACAfSPAC'SPACAfSVER4.0SVER FSVER,,fScal@Scan3TUBEE6Tmpr<UserphARphCH(phDYbigphQLcphTRphTRf\mci2a>qN8>o Ueh,9n5g~Ri6(*24;x$}FNVUCs-113xT]o7U"y6^i_y>]y&m"o`^ kS\KQpYp&!gL-GI&7.{Mco6m~2HBIUbe1Cd}FxQZEZ,}4HUXYnuPQhoNePv7b{Wj2oFtXyy3@=?]p&)C^DZ=CHTA,h{|dsYuTF}wK[*oCfo;LY&p5S_3I|o6HvFHZ( g>}WqD@~TlW+b@cT]`Aq)4YU/L3!,Nl)!|3M[H#S_d.u?zdq3+fFc':Z^P?ZY{7+VDTj.uFgq%,NKo-,(B~bwj{}L]^k@_ajF=W.X}=";WjcHdkabCDkm[&HcXe"t}1%j-]Tsz^`A.u?s-u@G1fCy:*?GD8Y~Csk%pcyO_lh#m|!yrA]#7w"OMbq?Eb,Z#GlOjW^]=7l1%Em[.YYo%/D^*Pis|K/l3672'[)VBT9\qwH=qd)B*E\jE@r9#$%rnLjL3ro{`N%7`h?D,kSagv`k=U@xkk^CELhhSc%bVm? >S]*|QK:}$OEH3udrVbH`6@m r[%`,vfa`~>`% {=+&*IB9e R0]-jE(_y"DQX;:,292CjFnjUZ:U$n?XDD[ho*9wQi:A,fv7b{Wj2oFtqwGK"j+OC5wz^`Z]k7{d&]?#5ngDVD$3#2ps'Mvn[Ca,k?]mnjH[q?Eb,u2We:lFm%FHN] 5y$47y 4orKOR`{A,}@1%i`+|*+RB]9tuEJeRnFCQ_y!iU5|8e7sINi@C,_,qz7z}yaih?]R_s_bed{a9_ajFO-U[dUDNxGq@p'Ic62nrD5pY^d,/IQ}{sGitG3(|}jGPNYQIxw}H{-*<^k8e7?GOV5ro{`qafE*&aF2`_c&a6';_szT/"a/hV6T]==6X*s$[-T_sKQm5APYXA\95Lq>l>o 8:T3?qm#vRRC(vJayr=u4ZfoQe=N^tpu&ofW)NKog~!-|r< wvr]LaiY2]kO~JcW`$~~;`.pr$,.[G57'9\47.