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    ! /                                     dx,,,III^^^{{{&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR7y}|{ywurrpnlihgeOOOCGGddd{{{   #%%---555=>=EEELMLTTS[[[baahihnnnuuu{zz<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 8ad2a7dc092c49c889baa093e3713212Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-04-11_15-26_19693130Seq1011910119_Lehman_K1355_psbajmadera96-WellK1355_E02_psbaKB_3730_POP7_BDTv3.mobSeqPop7zZZZZZ[[\\\ZXVTTUWYZYWTPMLKJIGDBAABCB@;4-'%$&()**)(()*,,,+*))))*****+,,+*'&%%(*,-+)&#""#%&()+-/13579=CJS]jy9Z{ fApcXND;3,('(*,--+(&$#$%&'()****,/6?JWdtCu B_u~nR+i8 zgXNF?:88;BLXeu!e dm<am\- NNzy{}~}}B[Dn&-"KqYI?9543469=CN_zW;y>KmWJC?=?DPd3pR*%:EZ>-%#$%$"  #/@VofK4"  %5JaygO:(  /c=d|hHF.Y7b,q`TJA80)$! !##!.Kx i!`|p?-O+!&(($   N3_LUb~d_},1J    :p4^[+jK "!    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DN4 HN]'AGY[.c:aS(..D#JX9W9),^F*= RN5^5*: CH9[a^6!Y'U:HBN-YE6/1*SO< `Y'5:Ya<O (H'_R`G;, $ !C' F+-<TYX<UCGY/B.[ FJ.D- Ac,C8&3*(%2*4;B3[R<CF8AM%:8(LJ0J!+!,2R2@O8WU.%4 dVb-(I@K HIa+I=ALF ;^1NNRACZVM0H7/!JK]?*&9S# O (b0*&4.H5b)1V&APA*4EC2R.V.G;^%R[$<@5I^QU !F;",+\@W\DaPGF0!8_N8-0:5+3#Bc+ PH*$7 F K6 D!^H_2@O$^Cb%je7nik)H:+>=o\o vc>, ` H{Ow5i<Q, T:1OK,.*&fuIH7XnTHN.'&tuPZDhlh/MSuG:JX # <vRTn\']:p47[biF>4/I$,EJ[B'j,"$C^1YFM*`+JKvF,A  AP'Q]18@jT #}]-JfBpdv{j'w_.8lj4 SVT{ZMe=/6uP)w% Oj!GmQH=z|S3hsBf[K_vG}|qwVB=fy]" R-5}  H4B2~% V6/sg%avlOXc0 #>71!G5)HA=2.+'#$  ! 5*                       !              % % '  (('6  + !.  -,(1$# $1"!&1 /7%'61!&</CA46# */E2/>A<)""E$<J";>#7E"6@(8#0KAXOE>$F8>NO^5GI#IKKJ.KfOL;JHFN^:EC=ACLK/MMMM22/HMH22222=6ppB .S0d#j8O8)PGL<,T>XV,7clA8M1&d\wUpN[tZ7md5=tA=HMyE,"tpVQeMablR410,,`1G!82t+~t:BiQ|]@xL1KJ1@C,0:'H.sanHx28Q:8p1E,zd\&j,-sJ8w?p7(=FYI@8,vZC(p8gNpp Xp~pj SFxLOl4Uy\-YQbQ=bN e- 1?WMUO@v8FM8 ynR7<lq\E5!rW"ME7J'(&"!BHL+&(,@"f='.D2_#HJB7/,9;*A&W.Vb",[(-'//0 /'()-1#L#C%!-!4/#>8+;7H%4##)9%S#F&G!"'"D"Bn6I";E(  "D         #"" !^!>!( "-.%%B-*B#3')%"+4  "%,7 8-,75 ),A% 5*1'"96%1.*$!%+G%"((%!K$72V .,/J(`"1U?9),"B)1&4P:,= 1=(C*' @GB<8^P5 8`AAATCCTAACAATATGCGGTCTAGAGCTACGGATACTTATATATGACACCAACATAACAATATGTCTGTTCCCTCAGCCGATAAAACCCTAGGGAAGTACCCTTAGGCTTTCTCCCTAGTGTATTAGGGACCTGGTAAACAATCATATAAATAGCGGACGGAGGAGGGCGGGTGAAGGAAGGGGACGGTGGGTTAGGCGTCCCCGGAGAACGGGAAATAGCTAGCCCCGAGCGACCCTCCTAAGGGTGAGAAGTAACTCCTACACCCCGGCCCTCCCCACCAATCCCAGGGAGGCGGCCACATACCAACCTCCACCACCCCCCACCTAGTACGGTACGCGGAAGAGAGTACCCTTCTCCTCCTGTATCATGTGTGGGTTGGGGGGTGCGATGCCTAATCTCTCTACGTGAGGGAGAGCCGACCCTCGTTCCATAAATTACGCGTTAATTACCCCAGAGGCTACCCCGAGGGGACGGGGAGTTACCCCTATTCGTATTAGTGGCCCCGCAGACAGAGACTTGCATAACGACAGAGTATAGTGAGTATACAACTGCGACGGGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-04-11 16:56:07 -04:00AEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPtASPtB1PtB1PtBCTSG9BufT!B!CTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B^DATA!BDATA!BDATA!B$DATA &MDATA &M2bDATA &M2DATA &MDCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~ELIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOISP1AM1b1P1RL1b-P1WD1b6YP2AM1b?P2BA11CP2RL1b:PBAS11>PBAS11 PCON11+dPCON11)3PDMFrPDMFPLOC1b$PLOC1b oPSZE`PTYPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrN۹RPrV1RUND  RUND  RUND  RUND  RUNT RUNT 75RUNT 0,RUNT 7Rate RunN""S/N%SCAN3SMED SMLtSMPLcSPACAkSPACSPACAkSVER4.0SVER G.SVER,,fScal@Scan3TUBEE2Tmpr<UserphARphCHphDYbigphQLcphTRphTR&This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPtASPtB1PtB1PtBCTSG9BufT!B!CTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B^DATA!BDATA!BDATA!B$DATA &MDATA &M2bDATA &M2DATA &MDCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabUFVocUFWO_GATCFeat//UTGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~ELIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOISP1AM1b1P1RL1b-P1WD1b6YP2AM1b?P2BA11CP2RL1b:PBAS11>PBAS11 PCON11+dPCON11)3PDMFrPDMFPLOC1b$PLOC1b oPSZE`PTYPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrN۹RPrV1RUND  RUND  RUND  RUND  RUNT RUNT 75RUNT 0,RUNT 7Rate RunN""S/N%SCAN3SMED SMLtSMPLcSPACAkSPACSPACAkSVER4.0SVER G.SVER,,fScal@Scan3TUBEE2Tmpr<UserphARphCHphDYbigphQLcphTRphTRa,pEnO"#G)jp~0,W(YzX|s$4/YCC"*0RgKtj({W_zEUWo VgEM OkoWvWv9k]ty6Ka;` "Gq.aUPj(K3qoIH}7a2&d:Dp$^HAp2 ^m%bVm? VCCHY<6(mu y-WLa)&:\.~x|MI9?";!8L[2Rr`gPV]O_,~])RpikE:b1AmX-RMLr92U*a!(G2MZLNN1O3WAd@EaZL)E^rkrOW9;(dC18{ZWFrbT;fQGKekx&#v3"qo,D`&Tj1t9z"DEoh90S*o_cM&+#u?GG5)ljD_P?Yf]4'Uw\Pcc{\A'ZvXTP>I_n@(Zf.kRr+4#3y4E^h9r4uoak"T'o7LA;mq;;9VgO(_y"`k:!.tkSY4E2 (#W+6,j'zpTVy{BGk3_"Z2Kr)YW [xinJil]ZCd]'DZNu@v0kc "V&S$~=}F)h>;-T)RJTZmU)P? sIe]:Axe<>/D?*Uq$[-g]A8c[68Nv-OTVggi|}M|o9 nsu9-]H6X+9muy%lFU.F4/oawfT;GAzr|ik[}7-jAS_7WX7a^]*RiTK(>Vq&s1Hw,RI[xsU3T%ycX}O!DZNu@vulc*{d&]?#:{CsU|}&_[3A4zW2`&TA]BGmb,tJLV>BZuGZ-p@vddD\4ApZTFS$oak"T'oA_AN;)oX6YMI){mnjH[F.A[s:>ZCCd+4k,Z)lO[vHds<-F3x[R2LxC{IF}oH,k#_'>XA[.VJz$*#sejp~DsK2FtSEH$*YYo%rF(5,fX!Hiwe2{sGiSVqrj/.3~V'z(Z=kT/n@6TdO{7>kOptpuze/WR/X2EtERPV]O8oe])%,8Ea-cZ,f*F8B,}&8)TK7BKv'xPoQ`OY}]66{qT[sEF($}v4"/VAEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPtASPtAUDTllcB1PtB1PtBCTSG9BufT!B!CTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B^DATA!BDATA!BDATA!B$DATA &MDATA &M2bDATA &M2DATA &MDCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabUFVocUFWO_GATCFeat//UTGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~ELIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOISP1AM1b1P1RL1b-P1WD1b6YP2AM1b?P2BA11CP2RL1b:PBAS11>PBAS11 PCON11+dPCON11)3PDMFrPDMFPLOC1b$PLOC1b oPSZE`PTYPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrN۹RPrV1RUND  RUND  RUND  RUND  RUNT RUNT 75RUNT 0,RUNT 7Rate RunN""S/N%SCAN3SMED SMLtSMPLcSPACAkSPACSPACAkSVER4.0SVER G.SVER,,fScal@Scan3TUBEE2Tmpr<UserphARphCHphDYbigphQLcphTRphTR