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" !           !                                                                      dx,,,III^^^{{{&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR7y}|{ywurrpnlihgeOOOCGGddd{{{   #%%---555=>=EEELMLTTS[[[baahihnnnuuu{zz<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: a0a6fb2355454591a10b48346fc60fb4Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-04-11_15-26_19693130Seq1011910119_Lehman_K1355_psbajmadera96-WellK1355_B02_psbaKB_3730_POP7_BDTv3.mobSeqPop7z78:=AEGHFDCBBCCBA?><<<=>??=<:8765421/,*(%#!   !)08@GPZft*GdrS6iXLD?<84/+(%#" !!!"&+07@KZl%Mv9NZ^ZP?'|L o]PF@<9778:=AGP\mO~"^.r[I=51....049AM]r(Z_-cM;.$"&+4AWw^C  DTfRD:55:FZt"_0sy3DkP=/%     Ef. n *'XdJ[f6 4^B d]E>K 2ThjZ9 uN2  ;a[3(gIpX'~oNr#D ".9?>6) ;qYm"oan FpvON# (V(js4_.-8AJXuiZ{Xf!E#;Xw`: +Q3%;&0q9%8n( 3:\6!&3HiYl$P(  %4FVckkdVD/,[CZU4H! 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$IP-_ O U"0z~?[~e}{*W":[1\NYcZ6p G%(|jU:SBG$KphBMVJ3>E>"8 abwVq 0\|],IYq*;+su D+Haqo/;d>nBOU$[ GmY dl-Q/M ) =0ECws[AeK2,xS-r2Ol i$U $:a U  ec 4 :$3Bu!H@@nt, r;/ s\@2 0XY=f|#!4 %t k  !*EUpd.5n;m!Vh*(!I4Qsj<eP1 q` H"L +]I ?SKenXk-&x\6PM=dWKJCMPD ##*(?= p64W: 0dn1( *6.n\Xy UO\{QVGcRk{[poA U9Hg'a$(;ap POW)C/CFHf&}x!   qQZ>/!,1)Ng#"#W0Q3++"/1( .+.{,..2/ ,'!1J<JD%+7%  0$ $ )>55!,">$%%G&%%%%%%%%%!A93(#7M&1m%E*%0/@+KZWv-%ZY5) N(( 8?((k++'"7+G.6E??'??n+Oe]+6:pIpip1_- t~'umpE%N zmU0*p1K,:e' O3$}r`!JBMG3o-R6p Y QrMo?l0.F=rKOOEZG~)ii4dE B]fh+M|pr=p sbNQ-p*$1!>>%Ps[ DF5&C.1%Cw,w^zv0@ [ovp}pRg5phpnpH&LRpy\iH&7 dd-Ip7eL`4 ")KG^<U{5 X]s]eVZ=.Ps`BS&_ G[IA_x0rB/L y,KT_}/JSM`71/;s;hsckc}Tun}Q&"wKs83zBs%GJ7[?&.! rXF );A W<8)n?cxw69'uV(h}.[[^Cf Ukh'i|}%ltkuklhy:)tH=g)%]Y($1cB7*"/9shI3B>?1TUC0*v5h&mdS#o-Dun&*L#?Y)D8U%F-k&N${[qeXdLHKHW_7Q|{,};Qc dfJMX#bjJh`BmLbATCTCCAACAATATGCTAAGTAGAGCTACAACATCAATTGGGTAAAGAGCTGGTGGTAGATTATACAAGCGCTTGGGCTTATAGGGCCATACCAATAAAACTGGTTTAACAAGTCGTTGGGTGAAACTGCCTCCAGATTTGTTCAACTAGCAATTAATGCAGACCGTAAAGGCACTAGTTTAAATACAAAAACTTACCATTTTCTTATATTCAAAACAACAAACCGAAAATGGAAATTATACTTTAACTAAGGGTAGTATTAGTATTGTCTATTTGAACGATTTTAGCGAACTGGTTAAAGTCAATGGAGTGTGAATTTCCCATGCGAACAAGGTTCGGTGTGCGAGTCCAAGCTGCACCATTTCATAACGACGTGTAACCAAATTAATTTCCCACCTTCATTGCTTTCTTATTTGTCTAACTTCGCCCGGGGTTTTTGTTTTATGGAAGCATCTCCCCCTATTTTAGTTGGGCAAATAAGCCCGCCCCCTCGTGAGGGTTCTACGCGATAGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-04-11 16:56:12 -04:00AEPt3AEPt3APFN APXV2APrN _APrV1APrX}}hARTNASPFASPtASPtB1PtB1PtBCTSF BufT!B1CTIDCTNM CTOw!CTTL ,CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!BnDATA!BDATA!BDATA!B4DATA '2NdDATA '2Nd3DATA '2NdjDATA '2NdDCHTDSamDySN  Dye#DyeNDyeNDyeN"DyeN'DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~DLIMS!!5LNTD2LsrPaMCHNMODF VMODL3730NAVGNLNE`NOIS:OfScfP1AM2wP1RL.uP1WD6yP2AM>}P2BABP2RL:{PBAS nPBASmPCON,tPCON*sPDMFPDMFQPLOC&qPLOC"oPSZE`PTYP)PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND  RUND  RUND  RUND  RUNT RUNT 75RUNT 0,RUNT 7Rate RunN""S/N%2SCAN3SMED SMLtSMPLsSPACAi,SPACJSPACAi,SVER4.0SVER ESVER,,vSatd^Scal@Scan3TUBEB2Tmpr<UserphARphCHhphDYbigphQLcphTRphTRjThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN _APrV1APrX}}hARTNASPFASPtASPtB1PtB1PtBCTSF BufT!B1CTIDCTNM CTOw!CTTL ,CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!BnDATA!BDATA!BDATA!B4DATA '2NdDATA '2Nd3DATA '2NdjDATA '2NdDCHTDSamDySN  Dye#DyeNDyeNDyeN"DyeN'DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabTeFVocTTFWO_GATCFeat00T$GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~DLIMS!!5LNTD2LsrPaMCHNMODF VMODL3730NAVGNLNE`NOIS:OfScfP1AM2wP1RL.uP1WD6yP2AM>}P2BABP2RL:{PBAS nPBASmPCON,tPCON*sPDMFPDMFQPLOC&qPLOC"oPSZE`PTYP)PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND  RUND  RUND  RUND  RUNT RUNT 75RUNT 0,RUNT 7Rate RunN""S/N%2SCAN3SMED SMLtSMPLsSPACAi,SPACJSPACAi,SVER4.0SVER ESVER,,vSatd^Scal@Scan3TUBEB2Tmpr<UserphARphCHhphDYbigphQLcphTRphTRTrU]wfgM6v;(vJ4;iD4ZI4$%\e]yaQ>2nxHBmHle8hmYh^n_k@0{RRV3?wrmJGLbva].p>x'[IoOoq4^M1}GVRI<672_4CF_E[UO')JRr+4#Mc]_^ave>uDs5iqbyMimE%ToN6|1:#<'r{?:TA2oz:4JA^V\h}c%kU?-}U&0;S3ZfYUgB\(<({]oT2mt1A'obU2tRHef&e}hw@q}%Q`+N@c'WQwK m}{H1}z3/onxd:}ml]q:jJRWO[@*!$T]_`#?Q{6Jn`BT]{?v2FKeS5~i2oru-<7X_ Wl*ugmp]sUOzjge`HB! 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