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"                                                                  dx,,,III^^^{{{&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR7y}|{ywurrpnlihgeOOOCGGddd{{{   #%%---555=>=EEELMLTTS[[[baahihnnnuuu{zz<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 101abb8a6b414a9cb1b993734ce98318Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-04-11_15-26_19693130Seq1011910119_Lehman_K1355_psbajmadera96-WellK1355_A03_psbaKB_3730_POP7_BDTv3.mobSeqPop7z+++,-/122222234454322358:<;:7420//.,*'&%&(*+*($   (08?GNXeu3Le}v^C' n_QD:2-)'%#"!!"#$%%$#"! !$'+1:FVl 0V}{W3 lXH<3.*(()*,.17ANb|=7Fn fRpR<.&"!!""""""#$%%%%$#! !&.>XCU2QS7R4uO6'!!%-8Ic@7J1pK1   ;d8DC)Z6        CjDDmCK~U.ysFn]6a,(ShY}c/ !>n lJZC ^k="!$&(((&#   ,qt$q\Wy`EGoi8  4jn w?KQddv(     %UdA<E}:  \p*g3,@  0vep1 kPYr8^W$\@\BN       41R\AN           !irM`mW"=JyB&F      =pS4,q]      ,: ;x]   "(*("           4m2I< B       O6)\J}2            ;B*TNTP~b0       ,{rGc:NP )tFM/j4  T*7_Gv5 Q>hkI p5 A$`yi4O #Gqh@ :n X+W &O>}U" >O]Cj.!S5\]9i2 )Z<<N!/e$8)y@>4yV_(  $L}W-3`T,2oafl.!Kf9$S?@H>-h~k7Y'  .^yE;{"ibi+8mh=  3w3<<I3?*t<     Cv)MXI!rB   :_a>$ 'w>?_1%}q(   #&&! 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O5hqL^?WwFz2 #DpvmfcfpaF0"  %FtlC&  3_BYS3U1  {9AAALAvKB.bcpKB_3730_POP7_BDTv3.mobtermTATCTCTGACTAGCTGCTTTTGAGCTCGATCTACAAATGGGTAAGACTTTGGTCTTAGTGTATACAAGACCTTGAAATAAAGGAGCAATAACCAATTTCTTGTTATATCAAGGGTATTGTTATTGCTCCTTTATTTTTCTTCCATTTTTTTATTTACTACTTTTTTTTAGCCTTTTTTTAAATATTAAAAAAATGAATTTTAAAAAATTCATAAAGGCTATTTCTTCTTTTCTTTCCATTTTTTCAAAAATGGAAATTCTGCTAATATTTCGGTAGTTTTAAACTAGAGTATTGGGGCGGATGTAGCCAAGTGGATAAAGGCAGTGGATTGTGAATCCCCCATGCGCGATATCTCTGACTAGCTGCTTTTGAGCTCGATCTACAAATGGGTAAGACTTTGGTCTTAGTGTATACAAGACCTTGAAATAAAGGAGCAATAACCAATTTCTTGTTATATCAAGGGTATTGTTATTGCTCCTTTATTTTTCTTCCATTTTTTTATTTACTACTTTTTTTTAGCCTTTTTTTAAATATTAAAAAAATGAATTTTAAAAAATTCATAAAGGCTATTTCTTCTTTTCTTTCCATTTTTTCAAAAATGGAAATTCTGCTAATATTTCGGTAGTTTTAAACTAGAGTATTGGGGCGGATGTAGCCAAGTGGATAAAGGCAGTGGATTGTGAATCCCCCATGCGCGA+<V\oz,:JZdp|$.;ER^gs !+6@JVdq| #.8CNYdp{ !+6CMXer %0=IVakx)5?JWcmx !.:GS`lw    % 0 = J U ` m y    * 6 C N [ f q ~    ' 2 < H U a m y    ( 4 A N Y f q }   % 1 < G S _ k w  #0:FR^jv*6BNZgt~&2?LXer+<V\oz,:JZdp|$.;ER^gs !+6@JVdq| #.8CNYdp{ !+6CMXer %0=IVakx)5?JWcmx !.:GS`lw    % 0 = J U ` m y    * 6 C N [ f q ~    ' 2 < H U a m y    ( 4 A N Y f q }   % 1 < G S _ k w  #0:FR^jv*6BNZgt~&2?LXer %.::6#:*.>8>33*/8/>>,;;;>;$>>>>;;>>>;>>;>3;>>;;>;882886>>3>>>>>>>>>>>>>>;>>>>>>>>>>;>>>>;>>>6*>>;>>>>>>>;;>>>>>>>>>>>>>>>>>;>>>111;>>>>>>;>>>>>>>>>>;6>>>>>>>>*>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>;>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>6>>>>>>>>16>>>>>>>>>>>>/>>>>>>>>>>>>/1>>>>>>    %.::6#:*.>8>33*/8/>>,;;;>;$>>>>;;>>>;>>;>3;>>;;>;882886>>3>>>>>>>>>>>>>>;>>>>>>>>>>;>>>>;>>>6*>>;>>>>>>>;;>>>>>>>>>>>>>>>>>;>>>111;>>>>>>;>>>>>>>>>>;6>>>>>>>>*>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>;>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>6>>>>>>>>16>>>>>>>>>>>>/>>>>>>>>>>>>/1>>>>>>   8c.LNT_(D. S %P8OZ,E!S"7N GZ/AR8`2-&:4N7!bL-A_4"6Z4L D25ZMH+^BWdd0QE (:F KI#8WF(%O<+ EA5254O(Z0Z :8@6Q,+*'6@Rb?!+* Q5<52I3cSN aXOZ9*G7*)K70SX4V$+Q'[M *OdB+E1^DL):KO .aaV+LVZX*'%U@64 _8I>1< 11.N'9>QPZ1K'CIFP?b@&Y^,/'OG$"-4#NW`VL#5G6-N!9-:B7QSM'Q#\ I'>;o \?8o8$RqE(LcA,e Mnw j_fF[ljfUA wrO'k/Gvj' ^5`uLf=O!3oNt\$1Z$nu)D#_<}ji2 $Qeya%U`Ak;K0@Ann7=7YC>a5%nt>CB6J_xv]X#ywBM=gKx0NiPB  8 ljfF\?b|,] $}spz\f_WKNV}~M;7.*#.?2,# **.   .$)     %='0ppppcpp.u\K, <nQpp88$p1*C8t&+IC8/&)#6p5a/8.Wd8=g ex8!LDIVvBjCBJ{Tdg0+#wES,8pYn((^d+/8O-H=mpd,T|sQjEzi[;KtS>!zScT<0jZ$!M&<**' ,>XC@A)P+* #  " #60!     #'                 %#$1(     #  - 2)^mI;ATCTCTAACAATATGCGCAAGAGCGCTATATATACGTACATGTGACACCCCTGTCATAGTGTACACGACATCCTTGTATGGAAGCGCTATTAACTACCTCCTAATATAACGCCCCGAGAGGGACCGCTTCAATAAACCTGGTATGGAAAAAGAAAGGAGTCACAACCAGCGTGGGAAAAGGGAGAAGGGCCCTAAGTACCACGGGGTACGCAGGGACGCTACCTCCTAACCTCCCTTTACAAAAACGGGGGAAGGTACTCCTACTATACCGCAAGTACAATTCAATAGAACACCCCCGCATAAACCTTCGAACATACCGACGGACCATACCCCCTCAAAAACAAAAAAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-04-11 16:56:26 -04:00AEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPtASPtB1PtB1PtBCTS<BufT!B!CTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B^DATA!BDATA!BDATA!B$DATA 'ODATA 'O3DATA 'ODATA 'ODCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~;LIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOIS P1AM].P1RL],P1WD]1P2AM]6P2BA]]9P2RL]4CPBAS]]"PBAS]]!-PCON]]*PCON]])[PDMFrPDMF!PLOC]&PLOC]#PSZE`PTYPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrN۹RPrV1RUND  RUND  RUND  RUND  RUNT RUNT 75RUNT 0,RUNT 7Rate RunN""S/N% SCAN3SMED SMLtSMPLcSPACAxSPAC! SPACAxSVER4.0SVER <SVER,,fScal@Scan3TUBEA3Tmpr<UserphARphCH!(phDYbigphQLcphTRphTR RThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPtASPtB1PtB1PtBCTS<BufT!B!CTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!B^DATA!BDATA!BDATA!B$DATA 'ODATA 'O3DATA 'ODATA 'ODCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabJFVocJFWO_GATCFeat//JGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~;LIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOIS P1AM].P1RL],P1WD]1P2AM]6P2BA]]9P2RL]4CPBAS]]"PBAS]]!-PCON]]*PCON]])[PDMFrPDMF!PLOC]&PLOC]#PSZE`PTYPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrN۹RPrV1RUND  RUND  RUND  RUND  RUNT RUNT 75RUNT 0,RUNT 7Rate RunN""S/N% SCAN3SMED SMLtSMPLcSPACAxSPAC! SPACAxSVER4.0SVER <SVER,,fScal@Scan3TUBEA3Tmpr<UserphARphCH!(phDYbigphQLcphTRphTR&T`XDG7IG!Hgn+1Ab;*55p]?'KzK:*]Q{Y^@>=,8,XNKioh|m C]Z&|k1jK4|<0S1pPRuKojmqy=Y`gP#0beaA({]/wQ2a52rW.X}=1m)h>VO@0$^Xc:"RNLJ)TV1gM2{zO5+znJO!p8Npd-(bcnP6GZ]#(lCKOMQw T"S2Mmn[Ca,k +9}WQ@UzbaI23ttrRXc|LVn!<|'kU$3g;Z6Obl% eIe5_@{&]t[XS_Q7u2&q6X+9mvH%lFGTF4k}O!p8Npdpu#Wc4hAZG|JWIKT+}tr2LxCmqL}4|Mde_'61Qve])%,8=0?})jZ>zir8rP p'Ic62ir$1.XH`HO5QqE`|mwP@rB ]:]HE^OXv&nP_[O~}<"&eG1h3/[zGq;)y6}c8dgp3^|o:Xz zFA\|5DhECel7B]%jxxsWD@xG57'9\47.'s1Hhl@tt/rpi|3#xFsx$AWVM[~7Cpt`3AC?-4$&#S%TwKy3ofmq*6Y?Qs?\g>?{:LKu292CB|V&Xc|LVn!<|'@4o*MK>B%P^gWT^Fu*t&:_Gr9#(+gbk1^/.S3xCmqy=Y`gsB0PPOw[h@tC]Q#V>Ax`X~lTmt.qK|-o3o*oUnY!9s!&&v_QjXaM~3>>//x3L#Id4|Ca$"T'MT,Tc%ToN668d#