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"           !   d,,,III^^^{{{&&&999DDDXNXaaavvv      ***333G>>RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR=VVVNNNnnn  !#",--777@@@IIIRRR[[[cccllksss{{{<<<<;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 8c16148a62864214985a8bd4c81cfb77Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-02-15_10-36_17343130Seq18110137976_K1322_psbafjmadera96-Well$K1322_D3_Prescottia_stachyodes_psbafKB_3730_POP7_BDTv3.mobSeqPop7z$)+*(%$%(-1344334689;=>?@?>=<==>>>=>?ACEFFECBAABBCDEEDB@=;9888876420.,+*****+-/011/-)%"   $(-3:DQ`p2Pm{`C%r\K?71-(# $''&#""&+046679=DLSXZ[^gv'Ip6N\_U>OfJ3" !##""#'/8BKRW]gy'q,Cz6fmLTDDWY3(7FRXUF'z4qZH:0(&*4DZt7fq=uP2 "$',6Ig<og6a;" #&),/11/-**,18?EJKKHD@@IdayrpEo*nC%          &Yc'6{; !+5>DHJKKMPTZ`gnt{ )?d:D |m^O?,}uojc[RI@82,'"     (_UN^+   "Du<?'K$   :_|Q,      "#"!#->Wvo]RPRW\aeilnomjfa^\]`eilnmmllllkhc^XTRRRQOLGB=9643221/+%   !##!6v"s]W &P~Z8$%>m&U1%6_$& c=2Dp"Y1 .Z{N*  3OhzziR9#  <jP)  8_a:3UzlI*   &=XqqU8          )Nye>      2BMQNE7) *<LVXRF5%           /CT]]TE3#  #/9>>:1' !2CQYZUJ;,    &9L[c`TA-   8UoycG+   7Tp}fK2   !,6;<80&  2Mfx{kT;$ -7==7-  -?NWXQC3# *8BFC9,(3992( .G^nsl\F/"-8>@<3'  /?LTTM@/!(,,("   "(,+&  #()&  !*/1.)"  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M#=JLQy_>Ti.>7</N25XT)L&R)>HQx#!; #f\mJ\_4&11'=J%fg 5,cA,1/ )LyY}|nT:,H\# *r7%XtV!<-jDO)<B?P`&eVw=LGFNC+*PQ<P_US:20h\g%:=R3)bAwlL6^4`3J,UoQA &3N/_<AuW+X<DYI-dK/or3H+Akre[!M(Yu!(_;4Jr0EApYl^?-F}[|S5eI'p.9 .O3,Q]KEA:$+rMszM6M3G2 jPB.gksxT|`Q,1?\T6H%41/C*,H/O1QQ[7[tX8XylrB'yX{<RMuSBQlcb<H iiCbGais7DPV{gfgod~K`\=;QdjY&1kL)T>++DPamddsoLd@ZcPS, e-]c_FR0@68huEr@ c{%oN stJ Hs[Gd;ura0$7J+/{Q=RVtgc8K#v6"-]+D4}s:uB_Cm)AK}?^~4  IG#6/iSIl:Oh-LcHz/,v04UK_ Fx\mD-ULgPnO0i)xAAAAAACTCAATATATATCCTGTGTAGAGCTTTCTATAGGTAAATAGGACACCCACATACACACCTCAAACCCCCCTATAGAGGGGCCCCCCGTATCACCCATGAACTATATCCCCCTCCGAGGGGGACCTGGCCTATGAGCCCCCTTTCTTATATGGATATGCAGAGGGGGGAACGGAGCGTGAAGCGTCCCCTACGAAAAAGACCTGGGGGAACTAAAGCTTATCGGAGGCAACTGGTAAACGGCCCAGGGAGGGGAAAAAACAAGTTGTGTTGACTTGGACTCTGGCATCTTCCCGAGTTCCCGCTGTGGCCGACCCTCCCATTCTCCTCCGGTGGGCGAAGGGACTTTTACCCCTGTGATGAGAGTACTCTCACGTCCGGCCCGCATTGCGGACCTTTTCGACAACTACTAGTGCCTCGAGTAAATCCACTATTTGTAAGAAGAAGCGCCGAATCTTACTACGTCACAGCCAGCATTTTGTGTGGGGGACGTAGAACCAATCTCTACGGTTCAATAAGCAGAAAGCGTGTATCGACCGACCCTGTCCCGTATCCACCTAGACGATCCACAACCCCTCACCAACACCTCGGGCTTCTAGCACGAGGTGCGGGGGGGGAACCCTGTCGATTGAAGAATAATCCACCTGCCGAAGGAAACCCAGTGTGGGCGTATGACACGAAGTAACACTATCCCGGCCAAATACAAAAGAGGTGGTCGCTACTCTGCCGACGATACTCGCGTAGAAGTTTATCATCTGGTAGGTGTGTAACCTTGAGAGCCGCCATAACCCGCAGATATAGTTCAGGGGGGGGAGGTTATTCCCATCATCATATATGACAGCTCGCCGACATTAGCTCATGAAGGTCTATGTCAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-02-15 12:06:55 -05:00AEPt3AEPt3APFN APXV2APrN ŒAPrV1APrX}}•ARTNASPFASPtIASPtIB1PtHB1PtHBCTSrbBufT'NYCMNT UCTID0CTNM6CTOwICTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA )QRDATA )QR7DATA )QR0DATA )QRDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~pLIMS!!bLNTD2LsrPaMCHNMODF ƒMODL3730NAVGNLNE`NOIS/|P1AMnQP1RLnKP1WDnXP2AMnfP2BAnnmkP2RLn_PBASnn3PBASnn/PCONnnGPCONnnDCPDMFPDMF/PLOCn=gPLOCn6PSZE`PTYPQPXLBRGNm(RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  $RUNT  %RUNT  ;RUNT  +Rate RunN""S/N%/tSCAN3SMED SMLtSMPL%%SPACA]l-SPAC/SPACA]l-SVER4.0SVER rWSVER,,Scal@Scan3TUBED3Tmpr<UserphARphCH/phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN ŒAPrV1APrX}}•ARTNASPFASPtIASPtIB1PtHB1PtHBCTSrbBufT'NYCMNT UCTID0CTNM6CTOwICTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA )QRDATA )QR7DATA )QR0DATA )QRDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabFVocFWO_GATCFeat//}GTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~pLIMS!!bLNTD2LsrPaMCHNMODF ƒMODL3730NAVGNLNE`NOIS/|P1AMnQP1RLnKP1WDnXP2AMnfP2BAnnmkP2RLn_PBASnn3PBASnn/PCONnnGPCONnnDCPDMFPDMF/PLOCn=gPLOCn6PSZE`PTYPQPXLBRGNm(RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  $RUNT  %RUNT  ;RUNT  +Rate RunN""S/N%/tSCAN3SMED SMLtSMPL%%SPACA]l-SPAC/SPACA]l-SVER4.0SVER rWSVER,,Scal@Scan3TUBED3Tmpr<UserphARphCH/phDYbigphQLcphTRphTReih4qH4|t2#OoV$ zrTbZ?a23|jyHAj}W2yv!)QBmO7]N=]TG4/CeG1{?{sb: h,`,l6IEUY[^wA[h@t#RLk5/>RbS4}(FtSEH[!PYow.mU5s%+ .*;=8Es{jGyZ.T3d8A2r6fgh`K\A^O$['G=3/NwBx6_/<6R[Ule,rSBP^'6]`^ kS\KQp n$l^Wg#>z-nvP?QYMA67E |VzM:*]Q{Y^@>=,8,?qm#v6F[(vW3TA=]I:<{i(os1tog,s48h(PW[9?Db2@&'n[Nn%9SXWl573!dX!%~AfzvrfzoB=SUOuUgQjHbinOY5_}4]'>'s1HTf{fzja:3AG}X`=/r.ZNum#0kc "7:I12gbXW,62Q$OEHbX\"BV_@iD]xy"^O[,z^E$6d+XnhcjPGh{;qeXd9UZ#0AW$u;[[`&eg8gw'" 8+_@sb9, W,D>c^?8(F}z2g[jWcg;Z6Obe1Cd}Fp82)XaM~6>udrV|gwCiOfFc':Z^j?F?hECel7B]%j'kjWceOSx+C!K}Z'BNp{ e,&o.jrm$v5GK:.\_'[wA#Q9Tc T-*<^k'nwi5'S;.xhKn6g;X6YMI){wclQ[>A{%i/"a-cZ,f*F8B,}|O[nY?KPQh3f9b:O4S}=|s4v3[GH~X$,FleoBX}A.Okr2?fuT&-z*Rr,Z:X=<;s_|g+VFl@i2go~WR&^.9,(B~bHjAt3_iP""#]eRWJT|eiB]%j'k0WC.%ouO{9X6!7&o.ja8G$l=b d[qm#vRRC(vJnPX=<-8 )S-F3x@M9X`|W"[XYTZM%SmvlM@=K}VX*os7}Z&[Eb4;,#|>WT?@\xoF5K.Ijd!FwMz&4-@6|k.j,PCH-p}*4d#ky`[qDr_WG\|xT;#6RlSFy!{]-j5OPh~wA[h@t?"\=QtAcP@~~JBv+c8*+C!K}Z'BNp3<(+(aQQxnJilXS_d.u?zcw~CfF{*Tijh.iOW/D-]$/pTFT*u;SAEPt3AEPt3APFN APXV2APrN ŒAPrV1APrX}}•ARTNASPFASPtIASPtIAUDTB1PtHB1PtHBCTSrbBufT'NYCMNT UCTID0CTNM6CTOwICTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA )QRDATA )QR7DATA )QR0DATA )QRDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabFVocFWO_GATCFeat//}GTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~pLIMS!!bLNTD2LsrPaMCHNMODF ƒMODL3730NAVGNLNE`NOIS/|P1AMnQP1RLnKP1WDnXP2AMnfP2BAnnmkP2RLn_PBASnn3PBASnn/PCONnnGPCONnnDCPDMFPDMF/PLOCn=gPLOCn6PSZE`PTYPQPXLBRGNm(RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  $RUNT  %RUNT  ;RUNT  +Rate RunN""S/N%/tSCAN3SMED SMLtSMPL%%SPACA]l-SPAC/SPACA]l-SVER4.0SVER rWSVER,,Scal@Scan3TUBED3Tmpr<UserphARphCH/phDYbigphQLcphTRphTR