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"]4d,,,III^^^{{{&&&999DDDXXXaaavvv      ***333>>>RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRGRRRRRRRRRRRRRRRRRRRRRRRRRRRRGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRGRRRRRRRRRRRRRRRRRRRRRRRRRRGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRGRRRRRRRRRRRRRRRRRRRRRRRRRRRR=UUUMNNmmm   !!+++555>>>GGGPPPYYYaaaiiiqqqyyy<<<<;;;<<<<<<<;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: ca29dc6aeca8469f919012eebac19e95Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2017-01-11_18-14_16103130Seq17946137607_K1321_psbafjmadera96-Well!K1321_D1_Asteraceae_psba3f_psba3fKB_3730_POP7_BDTv3.mobSeqPop7z57;>@?8+ "3BKNLGB??BHMRUURMF@;98861+$ !%+00*! 0BPTK7         $''%! #)2=K\m}8MY_bcca[QA.uj`UJ>4-*))'$ %((((+06;?ACHSe (@R_fhe^RB0sV@3/27=@BFMYgt-Q ][6E)wni4zZI@>?@ACDEFFGGFC>70)# +:IV]`_^aofWp~2N8d>F\9&#.=N`p$i DWZK+t*rI*           $# 2FYit{W  L;n ?<n\vT_" " $,4?TzL>vK.     -@TbdZKBOt4:l<QeW effj)    0DWceZF1,H%gl,!+-'  "*8I\hhY>')_D>Z  !,460"F~CgR #vlfHo" $'""8g t2z R   '1;DKMI;$ dd V:!TJ         0=CA6%Q#R2Bu. 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K W c o {   $ 0 < H S ^ j v   % 2 = I T _ k x    + 6 A M Z e p }  ! , 8 D P \ i s ~ %1>IT`lx!,8DP\ht&1>IUakv+7CO[fq} %1=HT_jw+7BNZeq|%0=GS`lx ".;GR]iw,9CP[ht (4@QY"-6DMYbjq "-;I]ly *9ALXes| #/;DP]ky(4?JWdoz #.<FP[fq| ".;GT`kx'2>IUbo{ ".;EP[gs}  ' 3 ? K W c o {   $ 0 < H S ^ j v   % 2 = I T _ k x    + 6 A M Z e p }  ! , 8 D P \ i s ~ %1>IT`lx!,8DP\ht&1>IUakv+7CO[fq} %1=HT_jw+7BNZeq|%0=GS`lx ".;GR]iw,9CP[ht (4@QY   5*  2+/'>>*8*'&/'>8>;+;';>166>>>>;&>>;;3;;3;"""2888688;;>>>>>>>>;>>>>>>>>>>>>>>>>6;;>;;;80 68>1*68>88>8>;>>>>>>>>>>;>>>;;2;;1;;88>>>>>>>;>>;;;06;>6>;8;8>>>>>;>;;>>;68;;;2;;>>8>>>>>>;;;;;;>>>>>>>>;>>>>>;>6>;;;;>68;;>>>>>>>86;8386;;>>>>>>;8>>>>>>>>>;;>>>>>;>>>;83;>8>>>>>>3>>3>>>>>>>>>>>>66>;;>>>>>>>;>>>>>>>>>>>>>>>>>>>>.>>;>>>;>>>>>;>;;;;;>>>;;;8>8;;;8>>>8>>8>+>>>>>>>>>3>>>6>8888//+>>>88>88>-82>>/  +8/4+/*>>4+11>&>>>+6>.>!*6(-+)0*+1446%&(-"6:4/:466,."%'4#    5*  2+/'>>*8*'&/'>8>;+;';>166>>>>;&>>;;3;;3;"""2888688;;>>>>>>>>;>>>>>>>>>>>>>>>>6;;>;;;80 68>1*68>88>8>;>>>>>>>>>>;>>>;;2;;1;;88>>>>>>>;>>;;;06;>6>;8;8>>>>>;>;;>>;68;;;2;;>>8>>>>>>;;;;;;>>>>>>>>;>>>>>;>6>;;;;>68;;>>>>>>>86;8386;;>>>>>>;8>>>>>>>>>;;>>>>>;>>>;83;>8>>>>>>3>>3>>>>>>>>>>>>66>;;>>>>>>>;>>>>>>>>>>>>>>>>>>>>.>>;>>>;>>>>>;>;;;;;>>>;;;8>8;;;8>>>8>>8>+>>>>>>>>>3>>>6>8888//+>>>88>88>-82>>/  +8/4+/*>>4+11>&>>>+6>.>!*6(-+)0*+1446%&(-"6:4/:466,."%'4# + V,QG< 984<S+CA;'@c( /;J <I2 P B3_CZ"O7,GHBKN)G!`!0X4HN4@V_)Gc6'"`NJ0I?,329T.D?0\:_!2[ W,d B,J,N ,CHc;^?9Y/ON1/;Y9\dG('IPR=9Ka&4]5RY<\R8R&3& DS#!2A FO\#=*5V..,Q6dGY.D3`Q6SNc9)\ *RTV"W4,>'D\X> [Q`5`0QL <H.Y7RJ VXP$%90a>S,2[ [YB,R5C[ W&_-""NY <B%*"H\]X/a""3\> 58&DL3!M4:OYNA/ ,".!91ZQ*JP:0 -J+\W1X <bC X@ KNA.':2*]\+9HH6A"<@&R%&<P$/FSA1YY26c (P#W!c76X0,&4N,2W2:4TX-/:?s:#Z_]dxlDa,}%#V sls= aTuhVBAPv|+A3?egE$`Ew^Y;,m;n<rv!Hjk\O]:" 00&LV[ yVz YwtYquR\|*m[%R-a2> B,S;Q P<uG,'-<L6dD"V"yJ4vFd 7y?#)6>19nb{;1o Dyt`?Tgtp3zS>E{/~aaL(9VD/yC6r@Lh,_"}nrc.S7hXdg;"\  VRs~ t$&K.F"I^ZU#5ohB Y vO{ 1[Cl (H!wjrd;pLZMQQ:/C2N8?4 8)A%&,+6 <G!   3 (    18 $ ,   6',Qk" L8B$># H*DK-:%9$$7C/ ""219Z,-'c/ 5 ""<q#$5A#>"Z;7,442"4""4"fpCpppp'W9p7xF/0',iLr9z%0`PY ,s95D  `8eV*bkG ddup84do<wZ idiKkUg88f 8/w?<{SO{8PSg%*H*UUTCJ~C.opbdTM!>\NHTUXds z_*\z=s z"EodL,DLVp@p@bTp^9Z   6voNYZdv=O3';kuM.W U)UJl [;W+]IX"RFU$ DO-3.'8@?1*I8VgP-QI.9 ,_.:'~68$w*05 +9o5LfM.  !;*E"J,ES\B#8).C,3R#- +#t @oA/Eg(610zD212E<)B>Q'H$+9,)CYI.0t^1>H6,m0(8QMD&.3588Y59,!L6"V+6,-6 RK=K13t$E "=4,eA<-$U$+#,! },(A!/;!\'T',^TR#.@N2#6[2+[3 #;B+o86+1H+=#r#L+rcb$1>UT*E;&+%C8@h=+347)8B2AA<7SsTI0d[P]G^4c 3k(`FZmpdk2=IG^v[A=aDU4,o)I][^^\vtafF }EwAATAACTTTCTTACACATATGCGATAGAGATCTACATACACTTGCATATGACACCGATCTGATAGCATAGAAGCCCCAGAGCAATGGGGGAAGCACTACCGTCTTCTCGATATGCCTCGAGAAAGGCGCTCGCTCTTCCCCCAAATAAAGAACTATACGTGAACTTAGTCCCTCCACCTTTCCGGATATTACGATACGAGCCGCGCCTCCCCTCTCCGCATCCACGAAGATAAGGGTGAGGGTCTTCGCCTTTCTCTCCAATGAGGTGCCATACCTTCTAAGCTGCCTTTCGAGGTTATATAGTGGTAACCCTGAGGCTCCGAGAGCACTCGGCTGACACACGGGGCGAGTTAGCTGAAAGGCTCTCCGGCCAGGCGGGGGCAGAGGGAAGGGGTCTAGTGGTCATACTCGAGGGGACTCACACTATTATGAATTATTATAGAACCTCATATATCTACTTGTATCACGCCACAGTATAGTGAGTATTCTACCCGCCGACAGABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-01-11 20:02:07 -05:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTSPlBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA dDATA :DATA &DATA :u$TDATA :u$DATA :u$?DATA :u$BDCHTDSamDySN 0}Dye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0EVNT0*EVNT0=FWO_GATCGTyp!!0\HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~NLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS)nP1AM==P1RL9QP1WDA)P2AMIP2BALP2RLEPBAS+PBAS)PCON7[PCON5ePDMFTPDMF)PLOC1yPLOC-PSZE`PTYPJPXLBRGNmJRMXV1RMdNBiRMdV1RMdXByRPrNJ[RPrV1RUND  RUND  RUND  RUND  RUNT 4RUNT 4RUNT &RUNT *Rate 0PRunN""JkS/N%)fSCAN@SMED SMLtSMPL""TSPACAiSPAC)~SPACAiSVER4.0SVER PaSVER,,/Scal@Scan@TUBED1Tmpr<UserphARphCH)phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTSPlBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA dDATA :DATA &DATA :u$TDATA :u$DATA :u$?DATA :u$BDCHTDSamDySN 0}Dye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0EVNT0*EVNT0=FTab^FVoc^FWO_GATCFeat//^GTyp!!0\HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~NLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS)nP1AM==P1RL9QP1WDA)P2AMIP2BALP2RLEPBAS+PBAS)PCON7[PCON5ePDMFTPDMF)PLOC1yPLOC-PSZE`PTYPJPXLBRGNmJRMXV1RMdNBiRMdV1RMdXByRPrNJ[RPrV1RUND  RUND  RUND  RUND  RUNT 4RUNT 4RUNT &RUNT *Rate 0PRunN""JkS/N%)fSCAN@SMED SMLtSMPL""TSPACAiSPAC)~SPACAiSVER4.0SVER PaSVER,,/Scal@Scan@TUBED1Tmpr<UserphARphCH)phDYbigphQLcphTRphTRa,pEnO"#G)jp~0,W(YzX|s$4/YCC"*0RgKtj({W_zEUWo VgEM OkoWvWv9k]ty6Ka;` "Gq.aUPj(K3qoIH}7a2&d:Dp$^HAp2 ^m%bVm? 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