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"  6    $  %           $        "                !          #                                                                                                                     #                            <,,,III^^^{{{&&&/99DDDNNNaaavvv      ***333>>>RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR"RSSMNNmmm  !!!+++555???GHGPQPYYYbbbjjjrrqzzy !!!"""###$$$$%%%&&&&'''(((())))****+++,,,,----..../////0000111122222333334444555556666677777788888999999999::::::::::;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: b0de215dc0aa4fcca7290e5edfe6b8f5Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-01-18_11-54_16323130Seq17983137661_Lehman_dilution_testjmadera96-WellK1320_F1_10_psbafKB_3730_POP7_BDTv3.mobSeqPop7z~stChTUij=dNPh[32PeeP/   8ei?!!  $!1PcbN1/]/OkZkD;BLJ85b{U* 7EE8& #Gmr^Yr:htfM=B\s]J6fMDFOVWSH8&4c>1 y7/ wD^- ##   "2>?6$ *mM[%bWnJzB Di|tR%&j L'" ]#T!  &0/$ (0-  2=AACL\jobF% 9Wf_G'   *@NOA+  ""   ua {S<C$SQ"b`7  'WBTK*fX`}P/0\%DN'B   hC-&(-15<HYlz~vdL5&#.AVcbR8 )AR\^^ezp eKZ Em-3 r iMQ-tH2(" *485,   $075(:1n[d NK %14, 2L]^L- :{moYO1 p   u%X2so3    (g1"Ep_wPZ(dfj-*,! 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(35, *77* #;IF4     /ESTH3 ,GXZK2'?ORE. :LL9Fn~_4 (DTS@#%Zb. ! &Npy[4 (+)'')+(    1?C;($)$.Rp{pR- *41">W_S8   '24- 'BX_S8 1bvI  3FLD1  )9@;-  ")($/2,  #3<8+'( 3=9* !   #15-   %7A?2   (>JI;%   "!  0?D?2!  &CYaV>! &6OfpgJ$3Zst^;  .DRRC)@]_:@AAL AWKB.bcpKB_3730_POP7_BDTv3.mobtermGCCCCCTCTTTCTAAATTAGCTTGCTATTTGAGCTCCATCTACAAAATGGATAAGACTTTGGTCTGATTGTATTGTATAGGAGTTTTTGAACTAAAAAAGGAGCAATAGCTTCCCTCTTGTTTTTATAAAGAGGGCGTTATTGCTCCTTTTTTTATGTTAATGGAAAAAAATTATATAGTAATAGTAATACTATATAATTACTACTTATACTTACTATATAATATAACTAGACTAGATAATAGTATAGTAGAGGGGCGGATGTAGCCAAGTGGATCAAGGCAGTGGATTGTGAATCCACCATGCGCGAAGGCCCCCTCTTTCTAAATTAGCTTGCTATTTGAGCTCCATCTACAAAATGGATAAGACTTTGGTCTGATTGTATTGTATAGGAGTTTTTGAACTAAAAAAGGAGCAATAGCTTCCCTCTTGTTTTTATAAAGAGGGCGTTATTGCTCCTTTTTTTATGTTAATGGAAAAAAATTATATAGTAATAGTAATACTATATAATTACTACTTATACTTACTATATAATATAACTAGACTAGATAATAGTATAGTAGAGGGGCGGATGTAGCCAAGTGGATCAAGGCAGTGGATTGTGAATCCACCATGCGCGAAG $-:IS\es!+5<GZjv#1;FQ[lt $09FT_lu *4@JWbmx)5ANZfs~,9DOZgs%1<HR`mz (3?JUbo{   , 8 D P \ j u    & 1 = I U a n x   " . 8 D P ] i t   , 8 B O Z g s     * 4 A L Y f r }  &1< $-:IS\es!+5<GZjv#1;FQ[lt $09FT_lu *4@JWbmx)5ANZfs~,9DOZgs%1<HR`mz (3?JUbo{   , 8 D P \ j u    & 1 = I U a n x   " . 8 D P ] i t   , 8 B O Z g s     * 4 A L Y f r }  &1<   .'& /3* +"  ($ 0;;;;38;+1;;;1'1"#""!.1++1>;332.8;8;>;8;283.06.2.8$$.8;$2233;>>;;;;;;;>>;3;;;8221180+#;11;6608;311$&11;;2#;8;;;)++"$"$2388;82;8;8>>>;>18028283;;;2;;1211.211..2."33;;283";1;8888;'" +3';""31;11+#"(&'8+,!"("311(+"$13+ "   .'& /3* +"  ($ 0;;;;38;+1;;;1'1"#""!.1++1>;332.8;8;>;8;283.06.2.8$$.8;$2233;>>;;;;;;;>>;3;;;8221180+#;11;6608;311$&11;;2#;8;;;)++"$"$2388;82;8;8>>>;>18028283;;;2;;1211.211..2."33;;283";1;8888;'" +3';""31;11+#"(&'8+,!"("311(+"$13+ "6?X"9E!'U;H%/dJB]bETSM1M"(&4Z/1_`7' X35+X"` G1MA)/]A86E(!#cEHAMNMdYO%. )4G$ b</ZHP  *L/\[X`^8O\1&a'3!E%9 =A0@]=$=Z/GJ<K SSVHJ+FR;-C: +BU2Y +K>a?FY,-"I2N*J </'`9?bIU<#\ >DA+!.7_ZT>W<@FG;Z5#KV6 R+2\#,&B=R+&&)R92SINTBO9E6#)#+4Y-) <\K %bw yhY& \"q/XcK]/\  _3ypP~^|G_iNEoUP&-wS@3dn]z|\1Ik<Si&`]G]#,IW(oFXk.aU|tDmzGe]LI[\b!R;tt.@4ER~&D"?;, qk GY `E^ "xIM`U8;LJh}?s&:Z0d2HMXsKD yZz ")%3MLyf[1R *5%C 8a  .   ,C%#>U% Zv<p8)n8z+id9lp }Z,d-V[| tR@,N[Q,+a@W5 d_=A5,~,lpJ!uV}xiN<1d#lY 4!?wh}#bIpe,6,d&Fz y,f)^,r` DiA-(NSdC W%eRe&tgw#yED4zn)cA:vu%DaW,~Nc\!qs<2<WUJ2>ZUV{+_mxkG7US)a&G^rUB0^W*!.3[d]A0;U[`:6KyOQcYSjE:jhL@Z:>@E[0R{X:z:?7d:3M@iCenvG?VS[K{0I:iS6R>k:WLkAvJYUK]-yg]&_+>xB+N rUq%}Z AAAAAAAACCCTAGGCCAGATGGCTATAAGTGCTCTACATACACTTTATTTGGGAGAGGGCCGTGCTACTATACCCCACATTAAAGAGACGAACGTTTGTACCTCTAGTTCCGTTCTCCTCAAAATAGGTAGCCCTTGATAACAAATGGCGCAGCCACCCTGACTTGCGCTCCTACCCCATCATTGTTCCTACATCCTCAGGAGGGGTGTTGCTTGTCTCTCCTGGCAAGATTCCTCATTCTTCCACCCTACCATTTTATCTGGTTTCCTCTTTGGGGCAAGAGACGGGTATTTCTTTAATGCATAATCABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-01-18 13:41:49 -05:00AEPt3AEPt3APFN mAPXV2APrN ȜAPrV1APrX}}ȥARTNASPFASPtmASPtmB1PtlB1PtlBCTSZLBufT rCMNT eCTID@CTNMFCTOwbCTTL \CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA 0DATA DATA DATA *UDvDATA *UDADATA *UDDATA *UDBDCHTDSamDySN =Dye#DyeNHDyeNMDyeNRDyeNWDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~XLIMS!!rLNTD2LsrPaMCHNMODF ȓMODL3730NAVGNLNE`NOISAP1AM6lMP1RL6lKqP1WD6lPIP2AM6lU!P2BA66WP2RL6lRPBAS66BPBAS66APCON66J;PCON66IPDMFVPDMFAPLOC6lFPLOC6lD-PSZE`PTYPjPXLBRGNm8RMXV1RMdN "RMdV1RMdX+RPrNRPrV1RUND RUND RUND RUND RUNT  6%RUNT  )RUNT  "-RUNT  )Rate RunN""S/N%ASCAN3SMED SMLtSMPLDSPACMSPACASPACMSVER4.0SVER ZASVER,,ǦScal@Scan3TUBEA3Tmpr<UserphARphCHAphDYbigphQLcphTRphTR76This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN mAPXV2APrN ȜAPrV1APrX}}ȥARTNASPFASPtmASPtmB1PtlB1PtlBCTSZLBufT rCMNT eCTID@CTNMFCTOwbCTTL \CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA 0DATA DATA DATA *UDvDATA *UDADATA *UDDATA *UDBDCHTDSamDySN =Dye#DyeNHDyeNMDyeNRDyeNWDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabhFVochFWO_GATCFeat//hgGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~XLIMS!!rLNTD2LsrPaMCHNMODF ȓMODL3730NAVGNLNE`NOISAP1AM6lMP1RL6lKqP1WD6lPIP2AM6lU!P2BA66WP2RL6lRPBAS66BPBAS66APCON66J;PCON66IPDMFVPDMFAPLOC6lFPLOC6lD-PSZE`PTYPjPXLBRGNm8RMXV1RMdN "RMdV1RMdX+RPrNRPrV1RUND RUND RUND RUND RUNT  6%RUNT  )RUNT  "-RUNT  )Rate RunN""S/N%ASCAN3SMED SMLtSMPLDSPACMSPACASPACMSVER4.0SVER ZASVER,,ǦScal@Scan3TUBEA3Tmpr<UserphARphCHAphDYbigphQLcphTRphTRc:]FN}T3pb}045@u:r*nOroz*N^YEu'+=3yHSBrRLohL)N6PagEU0T6;e=Y"sWi|Cal(i#5h1g9%TFIO?z3e]/\(fp%Jp,@jJo{l(l>6,8efPmE$w^6Rwe7sE^Z{F9LwU$<;172+C~6bfm{l|?"wdp$^HApEnFiWb)jN-:d=iU~Hfzq?;o4O6JSt{44|Jf1Qo~[p[UR6? 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