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'   "  "  "2 ' &   '  -$ %/ !'   %   +  "  %#& * -   !$#  !       #0($  &"  "  #        $"  " %'   -  +    '"  ) "* " <,,,III^^^{{{&&&/99DDDNNNaaavvv      ***333>>>RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR"RSSMNNmmm  !!!+++555???GHGPQPYYYbbbjjjrrqzzy !!!"""###$$$$%%%&&&&'''(((())))****+++,,,,----..../////0000111122222333334444555556666677777788888999999999::::::::::;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 2df0b3fd4fc1426d820bc5c4fdf5f30dSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-01-18_11-54_16323130Seq17983137661_Lehman_dilution_testjmadera96-WellK1320_C2_nodilution_psbafKB_3730_POP7_BDTv3.mobSeqPop7z      !#)2=JXft9SmeB~kZL@6.)%! #-<N`p3bX-mUA1%$+28>CITd{+jALu~Z%BB_H:3/,*+09I`}6z"I8t ArM5'"(.2458@OfUT ;3rP;/(&'+3?Obz#f:myPB_<#       &,120,((1K{5l"i\ XR5z$hm)jm;     !" ,Gr?f!mD'   .V[0vAw/E2dXr;   $),+& ;T>8q*    !#%',:Vz (*u9  ;okQO !^Bg P.X! !#%%! .c8[OPA  !  0uR?}(4z[l9  Vy8@    ',,%KpG&Whb_    #[ kvh.    &Zh!r[~Q:ro2Qy6   '/1+ *aaw7@>9QJV&    .sKANw'      FFHJ       ,a0W\>zA  "TCF'g3  AXH<    'WHS8^" +yr<W5n? 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Iy{<*[[g=G:tsY|]B G!\a%/\@c%+o3  8ZX  5`$UI}oruG`6I1_n7B|"!M  :}.6"Q  -tN&[=^)4eB_0>(_xFV"\TEL2H=^Ky vvMrmt(M\33ndG  P rJ~6   .tC]z1#VJCweF@yUdF  &d"n W   /vJ:G>PB:S  O^7x5   R9l*  /f7[Z2R%"KRGM <}#ev=A*i8fT Z{|\!0ag5    'W=E+q9 L&_wi8X%  (]2Y]<m5  :v Hhc<h1  !J%{F C~ @YMM,4bKw|Z}?  +Vd6  )]0[eLL"          !CnzL&     !!    "#"! "%)-024679:;:::;>BFJLMMMNQUY]__^]]^`accbaabceeedcbbbbba`^\[ZZYWTQMLKMNNLHC>:8665431/-+)'%$#"   $g;WktqdQ;%zR'mVA.-BXlQV>t !-0){KRH\4 X0 }ungb__bfimpsx HvO:q%+$PHC vaQGA=<;:9876569=BHOU]gs>m'DSTG. zEzeVLEBCFNXeu!_PKD4v"x[I=61,($##%(*+*'$! "#$"   $-;Po QD:\xzTS{dH8115:?BA=86;LmL0EbSLSO' 3Z</UZ>]h4 !&+--(?{JN^,  !(-/,%,Y(\`): ^,          !"%'*,---.05:@CA:.!"DjjY&     ""Dv\mW"    2;2~` N     $(,00-#1{oahy0        #')' )tootI#'+% S&!qbM           NKZ/b? #  O!aq@^W\x G            GBLIu3      $('B <O'_:  P~!i3 !&'& I^vQ|<  Ch"^# !" $g9 fJ      M,ewM G!      %V>B$g>9ZNz.G  ,[T-  GxX/         F9s%l%_f?-)+*$'QD:rv6zNxI."!&-5;><5,$ $(($1e2`qb6wH+$,0.'6eSl[D>BHKG>2&'4CQ[_[O>*         n@AHAC A KB.bcpKB_3730_POP7_BDTv3.mobtermCCAATCGTCAAGACTAGCTGCTGTTGAGTTCCATCTATCAATGGATAAGATTTCGGTCTTAATGTATATGAGTTCTTGAAAGTAAAGGAGCAATAACCAATTTCTTGTTTTCTCAAGAGGATTGGTATTGCTCCTTTATTATGAAAAAATTTTACATAAGTTTTTTTTCTAATTTCTACTTCTACATAGTTAGTTATAAGGTAGGGGGGCGGATGTAGCCAAGTGGATCAAGGCAGTGGATTGTGAATCCACAATGCGCACAACCAATCGTCAAGACTAGCTGCTGTTGAGTTCCATCTATCAATGGATAAGATTTCGGTCTTAATGTATATGAGTTCTTGAAAGTAAAGGAGCAATAACCAATTTCTTGTTTTCTCAAGAGGATTGGTATTGCTCCTTTATTATGAAAAAATTTTACATAAGTTTTTTTTCTAATTTCTACTTCTACATAGTTAGTTATAAGGTAGGGGGGCGGATGTAGCCAAGTGGATCAAGGCAGTGGATTGTGAATCCACAATGCGCACAA!2CK_dt{ )5BUcoz#18EP]kr!-6BM\iv'4AKVbmw ,8DO[gq{ #/<GS`mz &2>HS_kw,8DQ[gs    ( 3 ? J V c p |    ( 4 ? K X c o {  $ / < J T ` l x    + 7 D P c!2CK_dt{ )5BUcoz#18EP]kr!-6BM\iv'4AKVbmw ,8DO[gq{ #/<GS`mz &2>HS_kw,8DQ[gs    ( 3 ? J V c p |    ( 4 ? K X c o {  $ / < J T ` l x    + 7 D P c  "%)(2&/$! .:*.'*+1:>14 >>>>#8>.%>>)1>//8&>>8>1>;>>;>>>;>>>11;>>>>>>>>;>>>>>>>>>>>>>>>>>>>;>>1>>>>>;>>>>>>>>>>;>>>>>>>>>>;>>>>>>>>>>>>>>>1>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>/>>>>>>>>>>>>>/6>>>>>>>>8>8//>>>>>888     "%)(2&/$! .:*.'*+1:>14 >>>>#8>.%>>)1>//8&>>8>1>;>>;>>>;>>>11;>>>>>>>>;>>>>>>>>>>>>>>>>>>>;>>1>>>>>;>>>>>>>>>>;>>>>>>>>>>;>>>>>>>>>>>>>>>1>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>/>>>>>>>>>>>>>/6>>>>>>>>8>8//>>>>>888   G4]D6_=% M-AH1XZaIN3bc<.\U(7)]GT.DUY.1[[E)40MB=Q$ 4 ^R'F E%+A W01-J9%XV^WSM,3DE$DFX>VABOL\QX^Fc5c@"O>*a[H8$O]bM+,G2V]-O`DU1NY +?U,D 9E T4RPZcE]8#-+NQ?A+ca%6"S552N(5OaU( FT8BLAR%R?; &EY*TY'+YMX(c%>8` [`.]G$_~-m1Ggfm#k\a5&RK{m( ^R4TJ#wD uo.r^.zT:"`Pvdk,e;vM9! 3cPz,uUALwpKOG-m6*~4} |q 9Dm]y$QOxGE6 g!`hUl(!)zFt9BV N Ei"eCQP[ZA&Fnp.>z,*K.r2~rS8\n^sca@<6>>EA0_d5C;M:P0<C2 "'GL3;#,+'$)2-                             ! +Q 'p,8drS[i,(u5C}A81pLzpd 8%87'1`(K4pA$a dpPd8Zdd/g r5|p=d\!rO^yd;(z6pV}ehf&MX"[Sc`\~*~|#uQ02AU 1,X/L'1F$3-g2$3|&1$.&%"(0*(?'/?%;1<Q$-GC%4H//)0,!,4#$7  C-+   < + )E+&;%,#M' #$ *;&A*)$ ;)+*#D'7r4.)J)<Up@zh' AACCATCCTTTACAATATGCGGTGGAGAGCTACATATAACTAAATATGATACCGCTCTCATTATATATAAGCCCTCGAGGGTGTGGAAGAGTTATCAACTGCCTCGTGCCCTCACGAGAATAGATATAGCTCTTCAATAATGAGCGGGTAAAACATAGGTAACAAACCTATTACCTCCTCCTCCACAGTCAGAAATAGGATAGATTCCCGCATATAGCGACGTGCATAACGACGGTGAATAGTAACTCAACACGAAAAAGAGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-01-18 13:41:36 -05:00AEPt3AEPt3APFN uAPXV2APrN ȜAPrV1APrX}}ȥARTNASPFASPtASPtB1PtB1PtBCTS=BufT rCMNT eCTID@CTNMFCTOwbCTTL \CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA 0DATA DATA DATA '|N~DATA '|N;vDATA '|NnDATA '|NfDCHTDSamDySN =Dye#DyeNHDyeNMDyeNRDyeNWDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~<LIMS!!rLNTD2LsrPaMCHNMODF ȓMODL3730NAVGNLNE`NOIS(fP1AM2P1RL0P1WD4P2AM9 P2BA;P2RL6PBAS)PBAS(PCON/PCON.PDMF^PDMF(}PLOC,PLOC*PSZE`PTYPjPXLBRGNm8RMXV1RMdN "RMdV1RMdX+RPrNRPrV1RUND RUND RUND RUND RUNT  6%RUNT  )RUNT  "-RUNT  )Rate RunN""S/N%(^SCAN3SMED SMLtSMPLDSPACMSPAC(vSPACMSVER4.0SVER =SVER,,ǦScal@Scan3TUBEH1Tmpr<UserphARphCH(phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN uAPXV2APrN ȜAPrV1APrX}}ȥARTNASPFASPtASPtB1PtB1PtBCTS=BufT rCMNT eCTID@CTNMFCTOwbCTTL \CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA 0DATA DATA DATA '|N~DATA '|N;vDATA '|NnDATA '|NfDCHTDSamDySN =Dye#DyeNHDyeNMDyeNRDyeNWDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabLFVocKFWO_GATCFeat..KGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~<LIMS!!rLNTD2LsrPaMCHNMODF ȓMODL3730NAVGNLNE`NOIS(fP1AM2P1RL0P1WD4P2AM9 P2BA;P2RL6PBAS)PBAS(PCON/PCON.PDMF^PDMF(}PLOC,PLOC*PSZE`PTYPjPXLBRGNm8RMXV1RMdN "RMdV1RMdX+RPrNRPrV1RUND RUND RUND RUND RUNT  6%RUNT  )RUNT  "-RUNT  )Rate RunN""S/N%(^SCAN3SMED SMLtSMPLDSPACMSPAC(vSPACMSVER4.0SVER =SVER,,ǦScal@Scan3TUBEH1Tmpr<UserphARphCH(phDYbigphQLcphTRphTRRz0L/kbE!0z^n3HVQQEO2astcZ~~VGC2lB)lW+Uf066}ZC__cIBM}.z zFef&e}hw@q}%Q`+N@c'WQwK m}{H1}z3/onxd:}ml]q:jJRWO[@*!$T]_`P|Y;;y+Y3y'E3ro{`qaL`hlZZdBc'@wMXC5K;@WJG7jVlG0$_9'mSTveurDmCq?`DQ`+}{Uplql{]j6K8eX)GE#!#+X(fcT%GY?tY%%\2D>HM6Fnf%)q.i.]X"vQ*m2W@Vk1$#=4ZbZ4yM=VrTH&zqrua*b iq*#7rO/adu{>(Jt6qZ><}P?[NB@vulc*{w@p;#(":?S`,j.uFTcM#yKA#2rBA++mqIl*NSq|1n$fnwQJ*:["B (_e:RJ4ir$G(~}d M]f)C^DZ=<7:cmj^k-T0M5[S*.JpxcSX`"lef5U_9}|Ate1}1"/bW>y&bC]LVn!<|'k0|3.B+0Oj!Q4X+*flaO8JB6E$} {r*i` `VsIefaqQl&A6PG>}Oujd+[Y.m/wBN $`?"*J[x5FaGwpBT9\qwH=qd)B*vH%lF1?HOeCvpN^3jNRjnMgx*&`R@){wJUo6X?/WZ#&a:OcP$SXtmx+9Hgn<_swW^$buJrUlM;_}$A>e6bZ( iu2Zv&1`Z=WcDArh]|Ko5qS+SP7T]TcSI*$6ey(B~beaA({]/wQ2a52rW.X}=1m)h>VO@0$^Gc:FRNHJ0MVExMeIS-4L7=xU;C,6KP8)s5AVihwYc_`]:L]{RO7K57:=&CaF-B]%jyeY5+!hs|*>H~?p?}@8jaU(]y"/:Boz?xNu~)B*#qc3D44[_o5qS+SP7T]^q;,`h?&03S