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"17)6D9"3    #   *  ,! /+B=5   !7#$    (H6);A8=:8(DBI[hqwq^aI2%  R[FA.((.#/0?9CDP[JSDMF[d<>SL97F@<.;   7! -C*C<GVRTEOGK1"& 3/:hgu`sNF-2(-   X*,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR!UUUMMMlmm      **)444===FFFOOOWWW```hhgoopxwx~~}<<<<<;;;;;;;<<<<<;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: ba195f2200e0400cb9219cb403b1b0bfSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-01-27_14-37_16583130Seq18015137731_K1320_psbafjmadera96-Well%K1320_D5_Chaptalia_nutans_psbaf_psbafKB_3730_POP7_BDTv3.mobSeqPop7z !"##! %,5:;6/**3BUfprnga^`dhlnqv}}mb]]_beebZL:(%,-&    &+16<ADGLUf$>a'Kcr~lO,zB wpg[K:+"!'4BMQMC6) $+26873/,-3=KZix=t *5>B=+ o6re_^]ZSLFDHR^k{Z$0S(X^A n*+NiTJIMRVXXXY[\ZRG;2./353.'"!'/8>>8/$$3DVeq|'rgZ?cF3'"$+5?FMWk br GkrX`)[3        4`N,yD$n>R Sf)zuD)!%-4874/*%$%),-)!   ,WJo|oJh@$   FiDFj=>[(*|C    :}HeX$[     E\2rd-`$  (*%  2V uH3?nxEI <cBbeIM"   (f8OR>i63X3kd,_0 !"%(,/.(!#%%" !&('"!""    3d3ds]%XLsEJ0?  :b 52t?   >knD"      8vi]Zf    " .Zh:  :dq= &Uu^--[ `HU$  "Ew6_gJu6   daNtSr|1     Lm%^    )Ef`:BlS+  N0csZ G      6[{Z:    <gc9 .^ p6I[3   %XNt%y9!RA|z:6 $`2${C  -T~[5    (GgxY8 FzZ. 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")(                !-3/#                                                             "                        !%" fa@AFAS A KB.bcpKB_3730_POP7_BDTv3.mobtermGGGAGGCTCTGACTTAGCTGCTATCGAAGCTCCATCTACAAATGGATAAGACTTTGGTCTGATTGTATAGGAGTTTTGGAACTAAAAAAGGAGCAATAGCTTCCCTCTTGTTTTATCAAGAGGGCGTTATTGCTCCTTTTGTTATTTAGTAGTATTTGCCTTACATAGTTTCTTTAAAAATCGGGTTTTATTGTTTGGTTCGATTAGCGTGTTTTCTCTTTGTATTAATTTATAATATAGGTTTATATATCTTTTTCCCAATCTTTTATGAAGTTTTATTTCTAATTCAATTTCAATCTAAAATGGATAAAAATTCTAAATTTTTTATTTCTGAAATAAGAAAGAAATAATATGCTCGTTTTTTTCATGTTAATGGCAAAATATAGTAATACTAGATAATACTAGATAATAGTAGAGGGGATGATGGCTCATATTGGATGGAGGTAGTGAATTGTAAATCCACCCTGAACAGAGGGAACAGGGAGGCTCTGACTTAGCTGCTATCGAAGCTCCATCTACAAATGGATAAGACTTTGGTCTGATTGTATAGGAGTTTTGGAACTAAAAAAGGAGCAATAGCTTCCCTCTTGTTTTATCAAGAGGGCGTTATTGCTCCTTTTGTTATTTAGTAGTATTTGCCTTACATAGTTTCTTTAAAAATCGGGTTTTATTGTTTGGTTCGATTAGCGTGTTTTCTCTTTGTATTAATTTATAATATAGGTTTATATATCTTTTTCCCAATCTTTTATGAAGTTTTATTTCTAATTCAATTTCAATCTAAAATGGATAAAAATTCTAAATTTTTTATTTCTGAAATAAGAAAGAAATAATATGCTCGTTTTTTTCATGTTAATGGCAAAATATAGTAATACTAGATAATACTAGATAATAGTAGAGGGGATGATGGCTCATATTGGATGGAGGTAGTGAATTGTAAATCCACCCTGAACAGAGGGAACA&9DM_u|!)3@L`ep|-<DO[hv &3>HS`n| "-9DP]it)5ANZfq| "-9FR_jv)5BMXeq~&2?KValx   ! . : F S ` l x  ! - 8 D P ] i v  # . : E S ` l y    + 6 @ K X e q }  " / ; F T _ i v (5@KXer| $0<IU`ly ",8DPZfr})5?KWco{ .:FQ]hu*5AMZgt-5CNYgr~&5?KXdm~ &3AM[hz&9DM_u|!)3@L`ep|-<DO[hv &3>HS`n| "-9DP]it)5ANZfq| "-9FR_jv)5BMXeq~&2?KValx   ! . : F S ` l x  ! - 8 D P ] i v  # . : E S ` l y    + 6 @ K X e q }  " / ; F T _ i v (5@KXer| $0<IU`ly ",8DPZfr})5?KWco{ .:FQ]hu*5AMZgt-5CNYgr~&5?KXdm~ &3AM[hz  ' ()$!04 $6':: >>.)>>>1#>>6>) >>88>>>>/ % 68;1.3;>>>>>;10"2;>>>>>>>;>>>>>>>>>;;;$($+$6>;>>>;>>>>;>>>>>>>>;>;>>;;;>>>>>61>>>>>>>>>>.6>>>>>>>>>>88>;;;>>>>>>;820+1& 08+>>>>;>88>;>>>>>>>>>>;;>;>66>>8;882;;;8>>;>>>;>2.822;>>>>68.0;;8;;>>>;;28;;>>>>>>;888;;;;;>>;38;>;;88;;>2;>>>;>;>;>;;;;>>>8;8;;;>>>>;>;;;>3>662>;>>>>;6>>>>>;;;8>>>>>;>>88;>;>.&(8.3;;;;&2>>>;;+1,-88$.2-3#"- &+'            $$   ' ()$!04 $6':: >>.)>>>1#>>6>) >>88>>>>/ % 68;1.3;>>>>>;10"2;>>>>>>>;>>>>>>>>>;;;$($+$6>;>>>;>>>>;>>>>>>>>;>;>>;;;>>>>>61>>>>>>>>>>.6>>>>>>>>>>88>;;;>>>>>>;820+1& 08+>>>>;>88>;>>>>>>>>>>;;>;>66>>8;882;;;8>>;>>>;>2.822;>>>>68.0;;8;;>>>;;28;;>>>>>>;888;;;;;>>;38;>;;88;;>2;>>>;>;>;>;;;;>>>8;8;;;>>>>;>;;;>3>662>;>>>>;6>>>>>;;;8>>>>>;>>88;>;>.&(8.3;;;;&2>>>;;+1,-88$.2-3#"- &+'            $$  K\@KJ2>VYXO 3QB D`WM7WAc\A$7.$W SVc _Y4 b<6NB&`IHa1Z>Y `TEIIQ ]^N 'K(.b]\=GS?>1,0CVLF?;\+0(E5P, I[Y*B?K^'5+#>XBd4c(%%2 %bDR#/U$OS;-P?(A'>`=.D [?AZ(AYY<*>F:"N3#=>Z6D;1U>[^%=*cI?NYM2%!4(PMH("Q?NIK='@H6("6/%_'+ 2\U [\)bF!LE Q98>6=RSNT&Kd` 9M\#C[1#G+CY: <'D`K&'`J4JVQ6O^@4\`,]C"[,+9A0K PcIKXJ DN[+K;(Q1[<$K^"TGLXX'=(N DY LA%_)' F5SM(26N69]'_ .+_MR C"\2A4\_<Wa0 ;^-o YBao}K2,gs` v5i#pe\]s3F 57@[ABG?a,Ix8+f8_SojB $od|,*1v}`xy9pCH}y#arEn +VG&qiQSUNbIRJ4KrF=+ 8svi @VB<EApVR! F BIxG_Rl  pyAFH4;E#=LEW=Y@!lHUL!C  |;4{ Sp#-Q5VNid|v{6%r-e y2T5&Q> ~} GtPyia wU0 wao-:0"{MF3vvZc&fQC=$">%8 3XB(3;K';6.#% &&+%N5  # %  "  & !1   #AO1 *'>(" ""   -%-K*M -<8*! '1  %.P,  M1Q P_,,8!2IO;<'5'''&'$$#%|$*$''$1 '22222'? 2G$$'^pppdOZ+p-x0{"NXP`Q,k"[-;r;"i#p(89fX|NdJ,t5:X|N[~p]fJ|`8oi&-H`Km#p3p! (-Hd6sVQL,gL<"PpZ\G@ !r/&yPN8>d2WA,tpc*],0Lib zpGgg/<ejP!h^8;+[iLppELiXT+!*~;* 8L&^fc>y8CK*$*7)&5054-&6SF 79Vf=+:5JKQPnK;8'! 2E($0>'!e8PS-Q"Z0OO@+Y<[4;[0E]r8W;J2C)!G%6=4K./ %%*?&(KDESR$61(+0&&2!%l+V2VOeKpsYc/1$?>'02\AOD-?=7=88+#3g::7;2;3=/?!<N(N6?jV2/tX5F9D83-[GY5am53)F'5/"R(4/<761:|<C#@(&?FO2Q(9?89K2:2/4x5>`%$]9]?B/;SA,88L<)1*)R\uOXEDT[Aa%^7I<O<.65Lk&?4P9`0`cb3`4?3$8M2Hj' p.^elg|\}+ JmBDn[tj`!-2RH1 R ah!rMNB0WAAATATTCTAACACATATGTGATAGAGGATCTACATATACCTAATTAGGAGACCCCTGTCCTAGTATAGAAGACCCCTAGCAATGCCCTATGAATTAGAGGCTAACTCATAGCACCTCTAGAAAGAGCCCCCGCTAAACAACATACCTTATAATAGATTGCAGACGGAAGCTCGACCTTTCGCTAAAGACCATCCCAAAAACCAATCGCGAGAACACTCAGAACCCGTAAGGACTATACCTGCATACATATCCACCTTGCCCAGCCCTAAGGACCAGTAACAACCCCGCGGCGTCTAACCCCTCCCCCGTGGTCGTCCCTCCGCCCTACGTCCGCCATGCTGGAGCGAGGATAATCGCGGGAGGCACAAGAGGCCCAGCCTATCGCGCCCCTCCCGACCCCAGTTGGTGATAGGCCTTCCCGAGCATAGACATCCATGGCAGATCTAGTGGGATAGCTGTGTTGAATCCACACCCAGTTTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-01-27 16:08:25 -05:00AEPt3AEPt3APFN APXV2APrN ŒAPrV1APrX}}•ARTNASPFASPtASPtB1Pt B1Pt BCTSXBufT'NYCMNT UCTID0CTNM6CTOwICTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA )SDATA )S8DATA )S-DATA )SDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANE)LAST~~WHLIMS!!bLNTD2LsrPaMCHNMODF ƒMODL3730NAVGNLNE`NOIS3uP1AMFhP1RLBP1WDJ(P2AMQP2BAUhP2RLMPBAS5PBAS3PCON@PCON>PDMFPDMF3PLOC;(PLOC7hPSZE`PTYPQPXLBRGNm(RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT %+RUNT RUNT RUNT Rate RunN""S/N%3mSCAN3SMED SMLtSMPL&&SPACA^SPAC3SPACA^SVER4.0SVER XSVER,,Scal@Scan3TUBED5Tmpr<UserphARphCH3phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN ŒAPrV1APrX}}•ARTNASPFASPtASPtB1Pt B1Pt BCTSXBufT'NYCMNT UCTID0CTNM6CTOwICTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA )SDATA )S8DATA )S-DATA )SDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabg,FVocgFWO_GATCFeat//fGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANE)LAST~~WHLIMS!!bLNTD2LsrPaMCHNMODF ƒMODL3730NAVGNLNE`NOIS3uP1AMFhP1RLBP1WDJ(P2AMQP2BAUhP2RLMPBAS5PBAS3PCON@PCON>PDMFPDMF3PLOC;(PLOC7hPSZE`PTYPQPXLBRGNm(RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT %+RUNT RUNT RUNT Rate RunN""S/N%3mSCAN3SMED SMLtSMPL&&SPACA^SPAC3SPACA^SVER4.0SVER XSVER,,Scal@Scan3TUBED5Tmpr<UserphARphCH3phDYbigphQLcphTRphTRXPvc.[.u2Zv&1x5roz;P= =&e=1&!rE_g4Y@D}4r(>z1{zK90plkm$48v3}k76gg`q:QS#tyYZ(WxdUo`($'tV&ShKn6[M36YZ_SGmm{2}3Nuo2Ko5ry 6CfWBOjzF4#@u]3Fo'h]oP0c{yl1}ssJGH"GrM7VpCJ]wQ4=AY+-^m]ZOQM.jCPf{w&BEey069>R/Ury&EHtVeXciW 45=)ySQbir8r|:7'I3#wor({Y]?\/t]66{qT[sEAhiW?WUBs*u $O6%nOp$lYTPSD`^BnBCmJ1y~I,YtR%g`+m6L#BlHz24a^jmJ!]`-;z128)TK7BKv'xPP"F77jD8z{V-AMV^@Yo]p&9'*P7C]%2)$f&una`V!H|UUmbzee+n[Y<,h`Fzmj7a`<{!,8HW>y;`4E~G{WhcS]p?_sJOh^&6X`??}@8jbX-@^"qrB%P^gWTb*\mvH%lFDI| sYq-f^DR!_TcS{[}7&0E(_y"LAX;:#a92!ap(Z~~gln:tl|3[!]"oB/mn`kI Aq@E4/w!h,J)bIB?_'yBpUA!%EeKOWQ`QV-@[3>>B)u:[owQC(vJnPE*F;2_/gYFi$t_SF`|zvn=Y`1&!AuJUo6;^F6@_=!)}W}oo-o;X^`>EY$y!TW3nG6`VX+*fl{iG>A.8X:B?r@i$!^h{we**,ACT;fQGE-`x&i)6JWT|*0_fs$X=<;#(":?S`,j.uFTcM#yKA#2rBA++mqIl*NU4|1d$M*wH1*g4NB (_e:RJ4ir$G(~}d M]f)C^DZ=<7:cmj^k-T0M5[S*.JpxcSX`"lef5U_9}|Ate1}1"/bW>y&bC]LVn!<|'k0|3.B+0Oj!Q4X+*flt78{u,S]ZD>tSEH$*YYo%+0D5p>?e t}1%jF}up"GrM7VpCJT|Fu*qcjhAhE;qT%;5]U' S!oS\[Z*YaRsgGw>2Mcr.Lk@_aP4rRj"SD=y!.qK|N$t.DkFhwD~EG7&o.AiG3mwYB{,9)-]HqQIxw}H{-*PDMFPDMF3PLOC;(PLOC7hPSZE`PTYPQPXLBRGNm(RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT %+RUNT RUNT RUNT Rate RunN""S/N%3mSCAN3SMED SMLtSMPL&&SPACA^SPAC3SPACA^SVER4.0SVER XSVER,,Scal@Scan3TUBED5Tmpr<UserphARphCH3phDYbigphQLcphTRphTR