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(./" *,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR!UUUMMMlmm      **)444===FFFOOOWWW```hhgoopxwx~~}<<<<<;;;;;;;<<<<<;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: d108cd64a7d04db18ec87edf541876aaSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-01-27_14-37_16583130Seq18015137731_K1320_psbafjmadera96-Well-K1320_C1_Marattia_weinmanniifolia_psbaf_psbafKB_3730_POP7_BDTv3.mobSeqPop7z-)$(7FQWXVTTVZ]^^]]ajwticcjqurfTB63;IXdhe\QJHLRVVQIB@BGLONHB<98:>DJRZafiknt1H_tyW4Z4&:IPNE;2-+,,+(#$,7AJPSSQPOPSW]dlvCj rT8pL6,+.0/+&"#'-379=DN\kx|tmhea\SG=79AO]hpw (O|A!  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Ane=     !')$  &LtiE'    !.BZqw_A&             ,He|zcG-    0ZkC"  9`]<!     3Zg@  1QtkF* -P}b: %@afG0%*>[xoM++;HOL@/     -9AA<2(    0EWbd[K7"   3MgzwbG.)@ZqyhS=)         !4GV\XK9'         (Cb~pR6$"-AYmy{sdO:&  "8Rix{r`J5%"-?Uk|nS5  !  3JY^WF2!  1F[lutiWB/!                                                                                                                                                                             `u@iDA ASAKB.bcpKB_3730_POP7_BDTv3.mobtermCGCATTCTCTGATTAGCTTCTGTTGAGCTCCTTCCGTAAATGCGTAAAAAAAACAAAACCAATAGAAACTTGTTTCATTAAAAACTTTGGTCAAAAAATCAATGCTATTCGCAATGATTTTTGACCACGCAAATATTTCAGAACTTAGTTGAAAGGCTCGGAAACCTTTCAACTAGGTTCTGGAATAAAAAAAAAAAAAGTTCTAGTTAATATTTACTAATTCTTAAAATTTTGTTTGATTTTTCATCTTTCGTTTCATCTTTTGATAGAACTAAAAATTTCGTTACGTATTTACTTGAAAAAGAAGTTTCTATCGATTGAAAAAATAATTTCAAGAATTGAAGGATTCTTCTTTGTATCTCCCCATGGCTGGAAAAAAGAAGGCGGACGTAGCCAAGTGGATTAAGGCAGTGGATTGTGAATCCACCATGTTTTGATTTTTCGCATTCTCTGATTAGCTTCTGTTGAGCTCCTTCCGTAAATGCGTAAAAAAAACAAAACCAATAGAAACTTGTTTCATTAAAAACTTTGGTCAAAAAATCAATGCTATTCGCAATGATTTTTGACCACGCAAATATTTCAGAACTTAGTTGAAAGGCTCGGAAACCTTTCAACTAGGTTCTGGAATAAAAAAAAAAAAAGTTCTAGTTAATATTTACTAATTCTTAAAATTTTGTTTGATTTTTCATCTTTCGTTTCATCTTTTGATAGAACTAAAAATTTCGTTACGTATTTACTTGAAAAAGAAGTTTCTATCGATTGAAAAAATAATTTCAAGAATTGAAGGATTCTTCTTTGTATCTCCCCATGGCTGGAAAAAAGAAGGCGGACGTAGCCAAGTGGATTAAGGCAGTGGATTGTGAATCCACCATGTTTTGATTTTT(5BJWiv(7EQcq~ &2AMYdny $1@KWamz  -9GSbnz(3@MZfq| #/=HT`kw!-:GR]iu!+6BNYfs    % 2 = I T ` k w    ) 6 A L Y d p }  # / ; H S ^ k w    * 3 @ K Y e q }  " - 9 F R ^ k w !,7COZgs}(4?LVbny $0<HT^kw+7BMYfs~ %3?LVbn| #.;GT_lw+4ANVakv(5BJWiv(7EQcq~ &2AMYdny $1@KWamz  -9GSbnz(3@MZfq| #/=HT`kw!-:GR]iu!+6BNYfs    % 2 = I T ` k w    ) 6 A L Y d p }  # / ; H S ^ k w    * 3 @ K Y e q }  " - 9 F R ^ k w !,7COZgs}(4?LVbny $0<HT^kw+7BMYfs~ %3?LVbn| #.;GT_lw+4ANVakv %"#$ *  *4>66>*//8'6%3>>&1&>>2-+>>>>>1>>>;;>33;8>"83;;>>;>;>>;>;>>>>>>;;83;>8;>>>>2;;;>>666>>>>;;6.;.62'28.;8;;."6;;>>>;>>>;;;>>88>816;>>>>>>;;>>;;868;>;>>;>>>>>666.8000088;;;8;.8"'"&&8.;;8;+200.;;;86;;;8;+32&+(8882;32888660;;33'+008;28'"".)";;3+2(+828$&+1&23382288;18""" (" 23.".+.2"#."(2.2$".1382&'"";.+;8# 8>>;;;2283828'13&.1"". >>;33";33>(3"2-82,33;8",,&;8;;#;3388&    %"#$ *  *4>66>*//8'6%3>>&1&>>2-+>>>>>1>>>;;>33;8>"83;;>>;>;>>;>;>>>>>>;;83;>8;>>>>2;;;>>666>>>>;;6.;.62'28.;8;;."6;;>>>;>>>;;;>>88>816;>>>>>>;;>>;;868;>;>>;>>>>>666.8000088;;;8;.8"'"&&8.;;8;+200.;;;86;;;8;+32&+(8882;32888660;;33'+008;28'"".)";;3+2(+828$&+1&23382288;18""" (" 23.".+.2"#."(2.2$".1382&'"";.+;8# 8>>;;;2283828'13&.1"". >>;33";33>(3"2-82,33;8",,&;8;;#;3388&   #a V(LRCWA 47 =5#@RTH335T"V"E<86"X9@  6\J &FLY,)INN!6DWGD"SS$-U^ ZYA'3M=36d]M_ V[700D 8D(:")YFP@ .P=CYP:+4TZ['K>.FH cTK'%\% ,' )C26 6__R8[;. 49Nc6*P/KL2IK-:.aKNB>N$8 1L MW<2M?b2AOaLW(GOQSR^T\b /'REV RVYUIOUa3`$)P0T%]RHI: H$F/L<##F*VUJc.M^-1_1P a .7%YGU`P ;+Z"&![& / OaDKOD.F+ cL.?O b*R b/F[^Q[#?#EYJ A4!^`V Vcb K`6I5 DT*9J Z/`}^ p'SsfR4I*[ b;{7W85^MU[P'yUw|D2x\&On[MNh)G9BlO45WcwH]\6k+.zx_@?`H6 >yNjgr+U .{RwvD #> C+xYF5Pu@XU_ab~c3f6 UX_[4+D`@%bhh3*"NaO2U"su5s4>#nl}mPG]57d~oA>^x4t,jq*ySJ  (JH]g H=.g7!d =@Iz}YAZWX9^t9<9<9*GC$8<-M "H:*'(. N       /-! ( ';9>! .<*l-B  2% 'FM 7/ 8)%=!(X/ pM}pp(Xp=X10Ti+V Akp{~"DTc8Qe*&K-,Np7$p0);XXsQ3TbM(y}4p>dX<q N|QvUZoGg=T -o?$iB&6l=rAD%6i& wF.ytt,p',I>~-&ZIkLMdz5OyA"%uXI)FUa`sB!j&F#C?ec [B[hyB$udrhhm=PPlA{Wh9[a>0u6@,  7+04NK,DA&RVO0S/'DSk_2,p'!5=B $h&%%57#9.JVFaNog3*^e2?Kd"sf/i# Ek9#0"/Xa+8r'd('W18=)^#/03/W)N(9Espi,I'$I'/ UMRl#4yW~ok;=1n_ax=&SB\z6Ub=24X9]QFm_vO0XaVS^s?4UqLy@G:ruic*aZ}f_#ZAF@k,#l6I,OkJpO^'#3*ePZLS BU^_q,o9gfix%5h-KeTB1+9vB^:Alo}+G{wiPdE|n~}JeX7$2'0 |NWAAACAAACTAATAATATCCTGTGGAGATCTTCCTGCATTTACGTATTGGGCCCACCGCAACTATAGCCTCGTCCCACCAGTGGGTGGGATCACCCGGCCACTGCTATGCTCATTGCCCCCGGACGACGTTGCTATCGCACATTGGAGTGGATCGTCTCGCATTCATCCCACCTACCTACTGCATTAGTGGGCCCCCCCGAGCTAGTGAGTATAGACTCTCCCTCAGGGCACGGTCGGAGAAAGCACCTCCCACCCCACCTCAAGACAGAGCTCTGCTTGACTTGACGTATAAATTCGAGCTGGATCAGCCTCTCTCTCGTGTGTTTACCCCCACGACTCGTGGTATACTGCTCGGTATCTCAATACGAAGGCAGTTCCTACGACGCACGCACCTACTTGCTTCATCCCGCTGCATAGTGACTCTACAATATCCGCTTAAAAABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-01-27 16:08:12 -05:00AEPt3AEPt3APFN APXV2APrN ŒAPrV1APrX}}•ARTNASPFASPt,ASPt,B1Pt+B1Pt+BCTSKBufT'NYCMNT UCTID0CTNM6CTOwICTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA (QDATA (Q5DATA (QDATA (Q DCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~JLIMS!!bLNTD2LsrPaMCHNMODF ƒMODL3730NAVGNLNE`NOIS)P1AMt:P1RLt7 P1WDt>P2AMtDP2BAHdP2RLtA|PBAS+ PBAS)PPCON5fPCON3PDMFPDMF)4PLOCt08PLOCt,PSZE`PTYPQPXLBRGNm(RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT %+RUNT RUNT RUNT Rate RunN""S/N%)SCAN3SMED SMLtSMPL..SPACAc;fSPAC)-SPACAc;fSVER4.0SVER KSVER,,Scal@Scan3TUBEC1Tmpr<UserphARphCH)KphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN ŒAPrV1APrX}}•ARTNASPFASPt,ASPt,B1Pt+B1Pt+BCTSKBufT'NYCMNT UCTID0CTNM6CTOwICTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA (QDATA (Q5DATA (QDATA (Q DCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabZFVocYFWO_GATCFeat//YGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~JLIMS!!bLNTD2LsrPaMCHNMODF ƒMODL3730NAVGNLNE`NOIS)P1AMt:P1RLt7 P1WDt>P2AMtDP2BAHdP2RLtA|PBAS+ PBAS)PPCON5fPCON3PDMFPDMF)4PLOCt08PLOCt,PSZE`PTYPQPXLBRGNm(RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT %+RUNT RUNT RUNT Rate RunN""S/N%)SCAN3SMED SMLtSMPL..SPACAc;fSPAC)-SPACAc;fSVER4.0SVER KSVER,,Scal@Scan3TUBEC1Tmpr<UserphARphCH)KphDYbigphQLcphTRphTR,*^(`P. O~JcW`@0'q qoVU-9k *c@{UaG6EG>dcnxgJB7`DK,*Tijh.iOW/D-]$/pTFV2TVyLyD,kSagv9'i?oX$^io4)X^UM]|ny!.qK|C!^g:G1|*dc0U+O}L6iyNmwP@rB ]:l=&3#Veqy{g_hD6z\[N.6| Vkyc p7xMO9j;q6TIUwch.sQ@_=!)}W}oQln:~]a:WkR,uKsH"Kg5!O$AUI/vO3G`8hC0rOHJJr9b6mtTUk1T:29"5-z]T1S6RA0W7yLlo/&U{uclQ[vL}W$P1ObYn#pwW(YzXr$cn?:;wWG{uHYs+/e_'UvBVvwp#_T99Wq3F:{SocJy>@ YlGcRb[xy8J9tG>udrV=y`6@TmkcDynWa9?QLwUF3TP.&"D|;f[5kr+E{@2K/^1m*+9t\K5ej cS>e8:! jHW8I!Kx&549Pm3!,Nl7+n]iv65MT3 #pJE?RVuU,\)lOYW|_nw|]jL@A[]"dMI[h?&0E(_y"X_tXL ViY2]2rRU)>`VZI;P>W+YYo%+04NRY0GbR31}ssb[l"G>}O#pJl&v`VfXr9lKd,}Sn2M5FiKKB*oCaRZ^.EAx8aPI^QJH1}1"/]E]jWJDzbVFJ=`mcTv7KO]q6Po\s!&&v_QjXaq6.>`A?qm#vRRC(vJv:cGavedMNz/b_bRohj4TIx*&`OE!0z^n7aC<)\iyKG>?hECel7B]%j'k0$9eKoQD5pYHT+(aQQxnJilXSXW .C=vz^`%C]8p3iV3>g\]9L9qfLsGhOH+r,]_03BSmv^N6T.iP2i#G2rfJX|7"Wa%#;-T)RJ0vrs|%35uhwuRC{wkfWl@<{ZJ$Ge#a$tRwhA<%X#Ml(8wDo`p$AhVKubQ2a5NDzU4oFo;X.u8xXIc62nrD5pY(d+{o.jaFsA bXX<,x'EEwiOfF{\)lOY1|YL0eF`x&iJ-A|K7~57|NxOrz6 ?63_iPQ2aLtyc@"SD=;LX@HgX63g4`m^*Q1V'.$b[2$YWmOyl}$rEGCN8V+lwc7*<&YTIu{Qqk+^tpu"pcSIoM/V/5`agvLAX;:,aXj`Q Dx6m}aD&Wr`sEHu]4kkSnwkI6 +FwMz%FT!sz^`A(O>|ESRDujXTE-yh;}S$oa$N;172e,qz7|!f|bEdG[~{?26ttRHSK%}jx7Z]et|?%9'o *&AEPt3AEPt3APFN APXV2APrN ŒAPrV1APrX}}•ARTNASPFASPt,ASPt,AUDThB1Pt+B1Pt+BCTSKBufT'NYCMNT UCTID0CTNM6CTOwICTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA (QDATA (Q5DATA (QDATA (Q DCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabZFVocYFWO_GATCFeat//YGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~JLIMS!!bLNTD2LsrPaMCHNMODF ƒMODL3730NAVGNLNE`NOIS)P1AMt:P1RLt7 P1WDt>P2AMtDP2BAHdP2RLtA|PBAS+ PBAS)PPCON5fPCON3PDMFPDMF)4PLOCt08PLOCt,PSZE`PTYPQPXLBRGNm(RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT %+RUNT RUNT RUNT Rate RunN""S/N%)SCAN3SMED SMLtSMPL..SPACAc;fSPAC)-SPACAc;fSVER4.0SVER KSVER,,Scal@Scan3TUBEC1Tmpr<UserphARphCH)KphDYbigphQLcphTRphTR