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(E?9F@ :U@'=&)75(2O 6&  $0%-B $% ! !#D?/!< %5%=L0 6Mgnzz1.V%.,Gq|dkGh9-  +!,>9= $5  &AW\cxhc=81 ?LjPL {"   ) #/ {W              %EYT-)   Rr}TN9     !$                +016+)           $%%.*07<FNSkg\IibVjmXK_gWotnzCQ}<1EE eN%oXq|FrY#lgWl_ik9e_B vv\@564/( 37.0CJAb3EHR)vsD=>(,&".-RTOz!<?5#.sr?C0     #%1I\qOfh?:4GVv  "&w[a8!4 ! !6G^{xJL*3! !0gai[6:%*DDj|~zjW9$"   ENoyTUB (%$@/KXM_n\{woou]fegUO7<L%9   7-HLMN7K-  #N^+o #'O$FH. 3;5K+B8=% "*\RW^PY<<D1?MRYlwi\47C-7BMPLbVF>E)   (# 4EMpagX8;(  '   !   !-- #"8.,&,,2    &<901  %5ANEG;*30%8?CWQ@PZ6-(4*.( $<E4*- !.3  +  $     (>DZRO/#%%%    -!& 1" "$GFN=*     -,&/T8W@WQ8@>LMAK`Q@X?D6D9DBI\IAnW`S?M@/   D*9Lt}^jBI388[pfN:40, 0)  -38=K-'0hu.TYQWJuiiaB[k_UcH^99+%7=6`urkE1< *A`*,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR!UUUMMMlmm      **)444===FFFOOOWWW```hhgoopxwx~~}<<<<<;;;;;;;<<<<<;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: ee80c3a5a2c14a44b366a6e95b44943aSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-01-27_14-37_16583130Seq18015137731_K1320_psbafjmadera96-Well K1320_C12_Asteraceae_psbaf_psbafKB_3730_POP7_BDTv3.mobSeqPop7z3222221.("!*4=@<4+$"%,130)!#&'#$.43+ %%  #   #,.) !    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""                     &&                                                                                                  3PcfV:                    #" `[AA/\)Ar~(A*KB.bcpKB_3730_POP7_BDTv3.mobtermGAAAGTAAAGTCTACGGCCTGCTAGCTGCTTTGAGCTCCATCTACAATGGATAAGACTTTGGTCTGATTGTGATTGTATCCGAGATTTTGAACTAAAAAAAGGCGCAATAGCTTCCCTCTTGTTATATCAAGAGGCCGTTATTGCTCCTTTTTTTATTTAGTACTATTTATTTACAATGAAAATTTTATTTATTTATTACTTTTTTTCATTTCATAAAAAAAATAAATTTTAGGCTGTAATGGTTAATAGAAAAATCTAGTAATTATACTAGATAACTATACTATACAATTGAAGATGGAGGTTCAATAGCAGCTAAATCAGTGGATCAAGGATGAGTATTACGTTCATGCATAACGCGAGAAAGTAAAGTCTACGGCCTGCTAGCTGCTTTGAGCTCCATCTACAATGGATAAGACTTTGGTCTGATTGTGATTGTATCCGAGATTTTGAACTAAAAAAAGGCGCAATAGCTTCCCTCTTGTTATATCAAGAGGCCGTTATTGCTCCTTTTTTTATTTAGTACTATTTATTTACAATGAAAATTTTATTTATTTATTACTTTTTTTCATTTCATAAAAAAAATAAATTTTAGGCTGTAATGGTTAATAGAAAAATCTAGTAATTATACTAGATAACTATACTATACAATTGAAGATGGAGGTTCAATAGCAGCTAAATCAGTGGATCAAGGATGAGTATTACGTTCATGCATAACGCGA'<Qbv'7JW^p*3=EP[js|(5@O\gs !.;IRalv !.7>PZfq}'2=GS_hr})5?MZfr~ ,9DO\gt  # / < G S _ l t    + 7 C L Y c q {  ! / ; E T ^ m y    * 6 @ M Y e t ~   % / ; I T ` h w )5CP[jt#4>FR`my %0=GTany #'<Qbv'7JW^p*3=EP[js|(5@O\gs !.;IRalv !.7>PZfq}'2=GS_hr})5?MZfr~ ,9DO\gt  # / < G S _ l t    + 7 C L Y c q {  ! / ; E T ^ m y    * 6 @ M Y e t ~   % / ; I T ` h w )5CP[jt#4>FR`my %0=GTany #  * +   "61  '>86.;';;;!&.'1; .6.6 $6   "#0.+110!!  +22""    """8$$""""$$+$$-"#       $!"22 "  "   "  ! """ !1"&   "    "   " + ++"     * +   "61  '>86.;';;;!&.'1; .6.6 $6   "#0.+110!!  +22""    """8$$""""$$+$$-"#       $!"22 "  "   "  ! """ !1"&   "    "   " + ++"   SY%*`2M-R2-0Da1@1ES$B*A1-*#7W4OO"''L,WA MN?O)X*"!#-23#+Q\R< )#a;NI61"\&-R&(LUCFEOPROE#7(_- $10[)I<*7P$3:dY5*Y/ U;`DaDR![DL<]/>E!## LBS>0C %SLKY%5IAV:,9"RD 7%_+KMU*\3A+9aV,/bN*6GL% B "O]X+.19 682=a 9;63"<bSS!] ;9,U<AcKX%<E<! '3(b8OBY;C$OcN IK9AWA.(BWL;IP"1]2bW[<.B8d_=$( E-wu |xV4J l Ti s+XpSR_mt -0  QWOa7nfk>[ e j ab`FAQ+O_E|Wt*9B'+[OU1qPl+D}obb]Q]jl3$9]W67b>Gc8))nN3whE$ j :80Pq';6)\~4Qc ])zLf  5-q))2?p >5 1{[VbB /uIeptI5Q\XtI]PW<)UpcU:^01255B c E:6(AY{ ?9 Q  ( %N77~_Qa?}4,9)N  %;"$ " ^ L0 g p L 5K"*;=Kx85$$ E_w<#sSp0 |<}1`^ VNpI7L4G #6b- 8jO,v/c_g+>$@K &"ebog/z%a^/88}=8ybSa3=-3GDpCt.%pc1rRCdCd U(p$yJ$.EV|9.9)7O dB!a.DM[7).=&'-*6S%WY55 x1l4k6VNz86wWFah|cpukhw6XU$#_*0<nj_wF,[j1n<`kj"#1uw! b|rl:q|z.sg(d`\wQD+]JmoV E #$XGEnfQ\c@3|$N"(Jnl""(C]:W+7D \}*5d#^ CO1F M5 d56|KNopq0$c+|tMzeAGGTAAGCGAAAACAAATTAAAATATGTGAAGTGAGCTACCTATACTATATATGACACAGATGTGATAGTGTTAGTGTCAGCTATAAAGAGCAAGTTGGTCATAATCTATCGGGAATCTAGAGATAGAAGGAGTAAAACATAGTTCATCAAAAAATACCTAACACCCCATAGACACCCCTCGCGGGAGAGCGAAGTGCCTCCGGAGCTCAGAGCGGGCGGTTGATCGACAATACTAAATGGTTGGGGAGCGGTGTATCCCCCTGGTACTACATATGACTCTAATGCTTTAAATTAGGAACCCGGAGGGTTGTAGCCCGAATAAACGGAGGTTCAATTCGAAGTTAATCCATCATGGAAAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-01-27 16:08:57 -05:00AEPt3AEPt3APFN APXV2APrN ŒAPrV1APrX}}•ARTNASPFASPtASPtB1PtB1PtBCTSKBufT'NYCMNT UCTID0CTNM6CTOwICTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA )ERDATA )ER7rDATA )ERDATA )ER܆DCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANE\LAST~~JLIMS!!bLNTD2LsrPaMCHNMODF ƒMODL3730NAVGNLNE`NOIS/P1AMh=[P1RLh:P1WDh@+P2AMhEP2BAhhHP2RLhBPBAShh0PBAShh/KPCONhh9#PCONhh7PDMFPDMF//PLOCh4PLOCh2PSZE`PTYPQPXLBRGNm(RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT %+RUNT RUNT RUNT Rate RunN""S/N%/SCAN3SMED SMLtSMPL!!SPACMSPAC/(SPACMSVER4.0SVER KSVER,,Scal@Scan3TUBEC12Tmpr<UserphARphCH/FphDYbigphQLcphTRphTR/This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN ŒAPrV1APrX}}•ARTNASPFASPtASPtB1PtB1PtBCTSKBufT'NYCMNT UCTID0CTNM6CTOwICTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA )ERDATA )ER7rDATA )ERDATA )ER܆DCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabYFVocYFWO_GATCFeat..YGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANE\LAST~~JLIMS!!bLNTD2LsrPaMCHNMODF ƒMODL3730NAVGNLNE`NOIS/P1AMh=[P1RLh:P1WDh@+P2AMhEP2BAhhHP2RLhBPBAShh0PBAShh/KPCONhh9#PCONhh7PDMFPDMF//PLOCh4PLOCh2PSZE`PTYPQPXLBRGNm(RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT %+RUNT RUNT RUNT Rate RunN""S/N%/SCAN3SMED SMLtSMPL!!SPACMSPAC/(SPACMSVER4.0SVER KSVER,,Scal@Scan3TUBEC12Tmpr<UserphARphCH/FphDYbigphQLcphTRphTR0'"sIR*"$JQ`Q5f|{ce+dr6>svpkx5yu(p4-dId_$_;zay@Q>ko2Uz| Q-M2"ZwclQ[vL}W$620e:jA:>y7LIG!Hgn+1Ab;*5" ~Jz!#fn2+Xb[l"G>H1,Cz#Z|#e1a_y`[qD|3DG\TcpP.!3WN#4LdCh2d'jw~| imyZSH,6cXM{3!dX!%~Afzv*Cy KQI@'.%Nz7R@X`@kjJ}B~xGH$i*8j|]]u.ibu:,n_SUgi8i(=l27&mWAeAT%{AB02oGhnW"KuQtb'J!>yHPx%bg*p/Ygn7:I1~8U'W33EZ'nO$`j|"/WQ`Q`zX>ykI0o6Gzv&1NmbcnP_[O~}<"&eG1<@O(LxCmpD0,_03gPV]O8oe])%|2CWllme[OCy KJFU'.QHT/}]66{6sA bBT9zH'zeE^O{U,\)lOY%}WeoVL6z-Ik(~y5?X,]d:+PBH,&CW22Q&]Q#V>Ax7cXF$~y;|>EY|3.B|ux).u7z"$-m8)FTk2XS"j+OC5wz^`Mv,ZD-_p;s_|g+V3SKo)67:I1nK-k]|sOJr4{6QuA!9@1%ilJG82G [7P^E$62:gt4zuJiLBcYWgD-+T3.whA<%X#Ml(8wDo2($AhVKubQ2a5NDzU4oFo;X.u8xXIc62nrD5pY(d+{o.jaFsA bXX<,x'EEwiOfF{\)lOY1|YL0eF`x&iJ-A|K7~57|NxOrz6 ?63_iPQ2aLtyc@"SD=;LX@HgX63g4`m^*Q1V'.$b[2$YWmOyl}$rEGCN8V+lwc7*<&YTIu{Qqk+^tpu"pcSIoM/V/5`agvLAX;:,aXj`Q Dx6m}aD&Wr`sEHu]4kkSnwkI6 +FwMz%FT!sz^`A(O>|ESRDujXTE-yh;}S$oa$N;172e,qz7|!f|bEdG[~{?26ttRHSK%}jx7Z]et|?%9'o *&AEPt3AEPt3APFN APXV2APrN ŒAPrV1APrX}}•ARTNASPFASPtASPtAUDTgB1PtB1PtBCTSKBufT'NYCMNT UCTID0CTNM6CTOwICTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA )ERDATA )ER7rDATA )ERDATA )ER܆DCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabYFVocYFWO_GATCFeat..YGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANE\LAST~~JLIMS!!bLNTD2LsrPaMCHNMODF ƒMODL3730NAVGNLNE`NOIS/P1AMh=[P1RLh:P1WDh@+P2AMhEP2BAhhHP2RLhBPBAShh0PBAShh/KPCONhh9#PCONhh7PDMFPDMF//PLOCh4PLOCh2PSZE`PTYPQPXLBRGNm(RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT %+RUNT RUNT RUNT Rate RunN""S/N%/SCAN3SMED SMLtSMPL!!SPACMSPAC/(SPACMSVER4.0SVER KSVER,,Scal@Scan3TUBEC12Tmpr<UserphARphCH/FphDYbigphQLcphTRphTR