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***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR* RRLLLkkk%%%///888AAAJJJRRR[[Zcccjjjrrryy}&&'''''(((())))****++++,,,,----....///000001111222233334444555556666677777888899999999::::::::::;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 532763a00771472cae27db5a0d29df2aSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2017-01-26_13-12_16533130Seq18007137718_K1319_psbajmadera96-Well'K1319_G4_Cantinoa_mutabilis_psbaf_psbafKB_3730_POP7_BDTv3.mobSeqPop7z"  (3=@<0#"3BIC1 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"   "   &";1(+ #!#(#"8.22.&"+!($+""'#  = G63?T0-UHB;D1A0 [B6N$*A,aE &cZT#-Zc\RI=ED"OCS?'PSH*=LBHT"\_F.47RSS,W2NXQ& dAOE4I$,6#I(R$!+D94<7KCU5W&,J\I-%P\ :>ES4RC?!%&\)+FG:  1S 52@[, 4!R;Z)E(= _MI>` IS>c5B%Z?!0 S#H<9&44%Ya/) 5G0cSX;9 M9%`\FHCS<AY*,bF.JU )! <E!C ? tiVEazvrZ] Xq1BG a9(4Am=I6 V[,"z s~ KJ{xWw#xE 2D8%Ds }z8]Ze}zvlr[jZ"Jci|*5$Z*1\{b g sQ<]yxX ,$oVk7=,I%k9Bw A1eq[G(/7H"*QJ|%FWe4$_N:CRQD5oa )0#EUD 0A>0 0;M)?`" i*9-?3]3 ?[(+ ?  =e2 " ) #jZ3(^  4$p~p,!J\,pt>8RSY=l)a1pcg%'db ,zL3eHNxa)YR]$}("YKi!":,O6`9*3(,2(/Q8Zh_[v |: weezBjv)s'k ?#)[gqNI>)*@y;MZ1D>S'z5'@6.K5bA+el7!SM8BEE,Qi.#o299sKpD0^G*9}MRmU:=D>7Y0qKk?JX@`@qbeuqh8:UXDHKxJWzwj1:[\%O~h_LUFh}lzg,[ 2< v|@;t%CACTTACTTCTATAACTAGAGGTGATAGAGAGCTACACTTATCTAGGACAACCACTCGGAGTGTGTATGACCCCCCCTAATAGAGAGCTGTATGATGAGCTAGCTGGTCGCCAACCCCTTTAGGAGGGTACGGATCGAACATCCATCGGCTACAAACTGGGATGTCAGAGGATCAGACCTCTAAGTCGAGATTTCCCAAAGAGTAACCTGCCTATGGGGGTGTTTTCTCTCGCTTGTTTGGTGGTGTTGGGCGCACTCCCGCCCCTCGAACTTTACAACCTTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-01-26 15:12:16 -05:00AEPt:AEPt:APFN Z^APXV2APrN 5SAPrV1APrX}}5\ARTNASPFASPt<ASPt<B1Pt;B1Pt;BCTSZBufTy O-CMNT 5CTIDOCTNMO CTOwOCTTL 5 CpEPDATA@DATA@DATA@DATA@DATAy DATAy DATAy rDATAy )dDATA =/z^ZgDATA =/z^DATA =/z^O#DATA =/z^ɁDCHTDSamDySN 4Dye#DyeN4DyeN4DyeN5DyeN5DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 4EVNT 4EVNT4EVNT4FWO_GATCGTyp!!4HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~YLIMS!!5"LNTD2LsrPaMCHNMODF5CMODL3730NAVGNLNE`NOISCP1AM4OP1RL4LP1WD4QRP2AM4UP2BAWP2RL4SPBASE4PBASDPCONKPCONJPDMFZGPDMFCPLOC4HPLOC4FNPSZE`PTYPO%PXLBRGNmNRMXV1RMdNFRMdV1RMdXFRPrNNRPrV1RUND RUND RUND RUND RUNT  -RUNT  2RUNT  /#RUNT  7Rate 4RunN""NS/N%CSCAN@SMED SMLtSMPL((ZSPACMSPACCSPACMSVER4.0SVER ZSVER,,4VScal@Scan@TUBED4Tmpr<UserphARphCHDphDYbigphQLcphTRphTR'This is the confidence range *ABI_Limits..FVocAEPt:AEPt:APFN Z^APXV2APrN 5SAPrV1APrX}}5\ARTNASPFASPt<ASPt<B1Pt;B1Pt;BCTSZBufTy O-CMNT 5CTIDOCTNMO CTOwOCTTL 5 CpEPDATA@DATA@DATA@DATA@DATAy DATAy DATAy rDATAy )dDATA =/z^ZgDATA =/z^DATA =/z^O#DATA =/z^ɁDCHTDSamDySN 4Dye#DyeN4DyeN4DyeN5DyeN5DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 4EVNT 4EVNT4EVNT4FTabhFVochFWO_GATCFeat//hGTyp!!4HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~YLIMS!!5"LNTD2LsrPaMCHNMODF5CMODL3730NAVGNLNE`NOISCP1AM4OP1RL4LP1WD4QRP2AM4UP2BAWP2RL4SPBASE4PBASDPCONKPCONJPDMFZGPDMFCPLOC4HPLOC4FNPSZE`PTYPO%PXLBRGNmNRMXV1RMdNFRMdV1RMdXFRPrNNRPrV1RUND RUND RUND RUND RUNT  -RUNT  2RUNT  /#RUNT  7Rate 4RunN""NS/N%CSCAN@SMED SMLtSMPL((ZSPACMSPACCSPACMSVER4.0SVER ZSVER,,4VScal@Scan@TUBED4Tmpr<UserphARphCHDphDYbigphQLcphTRphTR-Dq.$+WH x:H-p9bgh`U*lO6viO$@I{-!`:ZPV2fI&$yNihB:Drj{H,:MX]#=c$DfqcdsTF]luKgk]35FQ6nB!X`7.w>6?xHf.QR9JK[YlyjSu30&rYA r3Oigi,$LzA\w/^ey:hN{+eqL}4|Md'ac_`k(U.*C]J=E]1|eKW3CNIG2gbXW,VE+0&'7%4X+*fl979v$sp3@=?]p&q!R;{?kJ:h+p?H(+Q;T`h3/[ze/G,y"o-,(B~b) e:=8}R"wKgpjm2D%~4r8h98&>LbsMvF(wD1RuM]UF+K4oB6Yl} /PuCaAR[(]f7u`GnTaioCp-WYJI*ys<7qL}4|MdwYc_`k:!.tko2KWtAcP@>/D?*Q| -4KD@4ir$G%1` b&}3672tX>VH~?p?}@8jVFTm[Po)9}P5O-!b+Smfc4P8U5ZfoQe=N^tpups'Mvn5mr+8/Yb~;HR;Lnl_[g(Vqb(nJy:]@|Q$OEH:6\"B-Y@iGtX,P> u)v=i57~+lF{YTl_{1#(7GDT`h3/[zr|R5/*7ag%gcmeaooUzbaI232)O~P@abHBa+b8EY59D4JWT|+adJsLl&pvedsgbk1^J.(KC4!7`h?&03BSmv^*:U[*WXWo0x9zG@}d>4"AEPt:AEPt:APFN Z^APXV2APrN 5SAPrV1APrX}}5\ARTNASPFASPt<ASPt<AUDTvB1Pt;B1Pt;BCTSZBufTy O-CMNT 5CTIDOCTNMO CTOwOCTTL 5 CpEPDATA@DATA@DATA@DATA@DATAy DATAy DATAy rDATAy )dDATA =/z^ZgDATA =/z^DATA =/z^O#DATA =/z^ɁDCHTDSamDySN 4Dye#DyeN4DyeN4DyeN5DyeN5DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 4EVNT 4EVNT4EVNT4FTabhFVochFWO_GATCFeat//hGTyp!!4HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~YLIMS!!5"LNTD2LsrPaMCHNMODF5CMODL3730NAVGNLNE`NOISCP1AM4OP1RL4LP1WD4QRP2AM4UP2BAWP2RL4SPBASE4PBASDPCONKPCONJPDMFZGPDMFCPLOC4HPLOC4FNPSZE`PTYPO%PXLBRGNmNRMXV1RMdNFRMdV1RMdXFRPrNNRPrV1RUND RUND RUND RUND RUNT  -RUNT  2RUNT  /#RUNT  7Rate 4RunN""NS/N%CSCAN@SMED SMLtSMPL((ZSPACMSPACCSPACMSVER4.0SVER ZSVER,,4VScal@Scan@TUBED4Tmpr<UserphARphCHDphDYbigphQLcphTRphTR