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(<,,,III^^^{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR* RRLLLkkk%%%///888AAAJJJRRR[[Zcccjjjrrryy}&&'''''(((())))****++++,,,,----....///000001111222233334444555556666677777888899999999::::::::::;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 7cfd9ce5412e43cfaf50dc61cacb2a61Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2017-01-26_13-12_16533130Seq18007137718_K1319_psbajmadera96-Well+K1319_F8_Cologania_broussonetii_psbaf_psbafKB_3730_POP7_BDTv3.mobSeqPop7z)*)'$$,49960*''*/46641014:@DGIIHGGHIKLNNMMMNPSUTQLGDDGJMLHA;522331,%   $(.6BRf|)=Tm~^;iTA2)$$'+--)#!'**)'&&'(('%%)1>N_p1Z"Y3 hUH>856:AJR[cm}^<J)FF(P8SEy (41~N}aL;/&! 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UJi6>       #&$2#oo'    &)'RAV~3  %&$ #]6*nt9Jc!        %('  %^"l;# :}C MB      %ZQXh2      5 in;IL|=#&"P `vQF         2w>fY%          'TCN6u<   S q{j4    %`ya_,  Cx#==!wD    @p $$ in*wu)R+ Aw/MM0~J&  9^c@$          3iXl!);T0"!&/:KdB^jfS4BgxmIfE2*)+,,*'#$/:CJLLHA:3,'"&Gw>E3 pJ0""@o9oi0qM:459;;:9741-++/6?GKJC7)                                       "A\A+AkA".KB.bcpKB_3730_POP7_BDTv3.mobtermAAATTTCTCTAGATCTAGCTGCGATTGAGCTCCGTCTATTAATGGATAAAATTTTGATCTTCAATTCCAAAAAGTAGATGAGTTTTTGAAAATAAAGGAGTAATATCAACTTTGTTGATATTGCTCCTTTACTTTTATTTTATTCGTAATCTAAATATTTAGAAATATTTATACATACGCTTTTTTTAGTTATTTCACGATTTTTTAGCAGTTTTTCCTCTGAAGGAATAAAGAAAGATAACGAAAGGGTAGAAATTGAAATATAGATCATTTTTACATTTTTACATAGTATAAGGGCGGATGTAGCCAAGTGGATCAAGGCAGTGGATTGTGAATCCACCATGCGCAGGAAAATTTCTCTAGATCTAGCTGCGATTGAGCTCCGTCTATTAATGGATAAAATTTTGATCTTCAATTCCAAAAAGTAGATGAGTTTTTGAAAATAAAGGAGTAATATCAACTTTGTTGATATTGCTCCTTTACTTTTATTTTATTCGTAATCTAAATATTTAGAAATATTTATACATACGCTTTTTTTAGTTATTTCACGATTTTTTAGCAGTTTTTCCTCTGAAGGAATAAAGAAAGATAACGAAAGGGTAGAAATTGAAATATAGATCATTTTTACATTTTTACATAGTATAAGGGCGGATGTAGCCAAGTGGATCAAGGCAGTGGATTGTGAATCCACCATGCGCAGGA"+4:Iafy (4ASdp| (5=JT_jt -9ALXdp|'4>HS]ht #0=JVcp| "-9ER^hs%1<HS^kx'4?KXes    & 0 < H V b n z  " . : F S ^ k w    % 1 < G T a m y    + 6 A L X d q |  " / 9 D O [ f r (3?KXcnz #/<IS`lx+8EQ_"+4:Iafy (4ASdp| (5=JT_jt -9ALXdp|'4>HS]ht #0=JVcp| "-9ER^hs%1<HS^kx'4?KXes    & 0 < H V b n z  " . : F S ^ k w    % 1 < G T a m y    + 6 A L X d q |  " / 9 D O [ f r (3?KXcnz #/<IS`lx+8EQ_   ":'5 -%64*#-$.>6>> >>>1*!>>>% 6>>> >>>>>>>;;&1>>>>>>>>;>>>;>>>>>;>>>>>>;;>>>>>>>>6>;;>;>6>>>>>>;>;>>>;>>>>;>>>>>>>>>>;>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>;>>>;>>>>;;;;>>>>>>>>>>>>>>>>>>>>>>;;>>>>>>>>>>>>>>//>>>>>>>>>>>>>>>>>>>>>>>>>>>1>>>>>>>>>>>>>>>>>>>>>>/>>>>>>>>>>18>>>>>>3>>>>>/6>>442+4$)4:4::44.   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D4@yA""= )2<-&-%#('"#"%/ 4'70<( 7& %'>T-h6C. $6 )(B/))'-!%&,=?(,..&"(0" & :" ""4' ')3 '$ &?F?%/B"9A%.!2:(%HY(:O'4#*Y>+C\]_dKhTTTAACTCTAGATATAGATGTGCTAGTGATCTGCCTATAATTAAATATGTTAAAGATATCCACTACAACCGGGTCCTTAAGAGGGAGAGGTTATGGAAGAATTATCACCTCGGTGGATATAGCGCTTCAACACAAATAAAATACGCAGGCTACGTAGGAAGAGGTATGAATACTTACGCTGAAAAAAGTGATCCCACGAGAAAAAAGCGGTGGCCCTTCTGAGGAAGTATTGATGGATATCTAGGGACTAGAGGTAGAGGTAGAGAGCATAAGAACTTGGAAACTTAGAATAGGATCGAATGAAGCTTCGTGTATAAGGAAGGTATAGGGTGTTGGTTCAAGGATAGGAACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-01-26 15:12:53 -05:00AEPt@AEPt@APFN ZbAPXV2APrN 5SAPrV1APrX}}5\ARTNASPFASPtASPtB1PtB1PtBCTSn4BufTy O-CMNT 5CTIDOCTNMO CTOwOCTTL 5 CpEPDATA@DATA@DATA@DATA@DATAy DATAy DATAy rDATAy )dDATA >}ZkDATA >}iDATA >}VgDATA >}eDCHTDSamDySN 4Dye#DyeN4DyeN4DyeN5DyeN5DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 4EVNT 4EVNT4EVNT4FWO_GATCGTyp!!4HCFGCEHCFGHCFGHCFG==InScInVtLANE\LAST~~lLIMS!!5"LNTD2LsrPaMCHNMODF5CMODL3730NAVGNLNE`NOISRkP1AM_`TP1RL_]P1WD_cP2AM_hP2BA__kLP2RL_ePBAS__SPBAS__RPCON__\7PCON__ZPDMFZKPDMFRPLOC_XPLOC_U\PSZE`PTYPO%PXLBRGNmNRMXV1RMdNFRMdV1RMdXFRPrNNRPrV1RUND RUND RUND RUND RUNT  -RUNT  2RUNT  /#RUNT  7Rate 4RunN""NS/N%RcSCAN@SMED SMLtSMPL,,ZSPACAsl6SPACR{SPACAsl6SVER4.0SVER n)SVER,,4VScal@Scan@TUBEC12Tmpr<UserphARphCHRphDYbigphQLcphTRphTRYThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN ZbAPXV2APrN 5SAPrV1APrX}}5\ARTNASPFASPtASPtB1PtB1PtBCTSn4BufTy O-CMNT 5CTIDOCTNMO CTOwOCTTL 5 CpEPDATA@DATA@DATA@DATA@DATAy DATAy DATAy rDATAy )dDATA >}ZkDATA >}iDATA >}VgDATA >}eDCHTDSamDySN 4Dye#DyeN4DyeN4DyeN5DyeN5DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 4EVNT 4EVNT4EVNT4FTab|FVoc|~FWO_GATCFeat//|OGTyp!!4HCFGCEHCFGHCFGHCFG==InScInVtLANE\LAST~~lLIMS!!5"LNTD2LsrPaMCHNMODF5CMODL3730NAVGNLNE`NOISRkP1AM_`TP1RL_]P1WD_cP2AM_hP2BA__kLP2RL_ePBAS__SPBAS__RPCON__\7PCON__ZPDMFZKPDMFRPLOC_XPLOC_U\PSZE`PTYPO%PXLBRGNmNRMXV1RMdNFRMdV1RMdXFRPrNNRPrV1RUND RUND RUND RUND RUNT  -RUNT  2RUNT  /#RUNT  7Rate 4RunN""NS/N%RcSCAN@SMED SMLtSMPL,,ZSPACAsl6SPACR{SPACAsl6SVER4.0SVER n)SVER,,4VScal@Scan@TUBEC12Tmpr<UserphARphCHRphDYbigphQLcphTRphTRA08^#5EN^D\we*S#ibu@Qe7EG>x'H1PSpj:6R}ag5d9w];H:'~%b9DBjh'&~o[k7MLr92U*a!ZCCd+4ko)Yr13.iOW/D-]$#{N.g\7dHm,_~/S9D>)ecH<]%9t}TU}xE_q-%Qvqs@dUa_e^W^4c Y \,yvMU:6%4sI}]66{6sA bBTd.u?zAw3+fc`]w>\fF4G&\%36Z=J*yc%F:!VO_5M%_|_n?NhN{C]J=E]V&=o>ZEZ,*`y{XW,@|DmOYXzwiKq1)m&Z Y6Gpve4WGe~ipFRd$K2LI/c{/'`gI2}__';_/Ft(#jFO-U[d1DFW%j'k0WC.%oKO> `VsIRD8z{!J!OpZIBoz?&qN^Fu*t":Ey+D;UTw|]jp&;2isfMFoM/V/5`agv`k';_szT/0p/(ZU&g"=6W\!3[s?RJAQ>KN73o ZbCd]ipPv6~MN#tH*J*q~|H~?pVRI<672tX>PG"BXP2gXk0[+D:rF(5,fc-,RIlVinJilZbCd]*>ZEx$DB+c7T-O)\rS|5qk`T1jdRC WIFM/|RB"H2UQFxRn)nZaK]lDs5ZW[HN? |3W'=yD^ZJYw SXE$^U4MOiiX>VL-&]S9Hgn_RDO6',>71B!Hiwe2{sGitG}ZkDATA >}iDATA >}VgDATA >}eDCHTDSamDySN 4Dye#DyeN4DyeN4DyeN5DyeN5DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 4EVNT 4EVNT4EVNT4FTab|FVoc|~FWO_GATCFeat//|OGTyp!!4HCFGCEHCFGHCFGHCFG==InScInVtLANE\LAST~~lLIMS!!5"LNTD2LsrPaMCHNMODF5CMODL3730NAVGNLNE`NOISRkP1AM_`TP1RL_]P1WD_cP2AM_hP2BA__kLP2RL_ePBAS__SPBAS__RPCON__\7PCON__ZPDMFZKPDMFRPLOC_XPLOC_U\PSZE`PTYPO%PXLBRGNmNRMXV1RMdNFRMdV1RMdXFRPrNNRPrV1RUND RUND RUND RUND RUNT  -RUNT  2RUNT  /#RUNT  7Rate 4RunN""NS/N%RcSCAN@SMED SMLtSMPL,,ZSPACAsl6SPACR{SPACAsl6SVER4.0SVER n)SVER,,4VScal@Scan@TUBEC12Tmpr<UserphARphCHRphDYbigphQLcphTRphTR