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RRLLLkkk%%%///888AAAJJJRRR[[Zcccjjjrrryy}&&'''''(((())))****++++,,,,----....///000001111222233334444555556666677777888899999999::::::::::;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 5743bbebfe3a4f4e8ef657535cc8e412Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2017-01-26_13-12_16533130Seq18007137718_K1319_psbajmadera96-WellK1319_E5_Solidago_psbaf_psbafKB_3730_POP7_BDTv3.mobSeqPop7zLLJHECBCDEECA@BGMSVUQJC<74214;EQ^gje[L>3.1:GS]bca]YWVURME<3,)+/59<;989=AC?6* %&*/,!!#"$-8FS^flpu}&<Sm  f=}qhaZPE90+-39>=80($%*05641/037<AEJOT[cp/RqfD${hZQMOT[dmxIx/GRK3 ~U.lO:+$"'.6<>=:74/*##)-.-++-047751,&##'/;FOTTPMO[u%{@R?H=ouR<22:FT_ehin~Z*]\3iRB7-%!"           &,/-&  &+-,+-5ANVWRNW^  bU&U`|iL*"&+0563,"!%'&$""#$#(Fw \;k>!  $'%!    )3<A>6-.K 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'     #     %           %   $  !   %*2 %#  7&$"%'") ('7&HP +(z,ppp9`)J]G\,d9Vd!8Uvy6d8KIpCdW3ld,ddupp OJdU,dl@gk28 6  ,{dlRk4,"8F 7.d,AZ0Y8Y,8* l^ @:pqpEdieVV; g:Y'8b$'-C843>!7Y*B 1@JdA81c0+*-S=! 3)947#)% .%<B8':(=:#9@4&-13-%"BBA.K  V=<B + '/ #@5*'>$LdC+(Kf4%"*@+Q*."+" %%+ &! #?(C,%<!,#W/l4$)#,$'7)2^3(4&:I 2/4"\~B&&}X7?;(gD]nCAAAACTCTATACACATAACATATAGAGATCTACATACACGTACATAGGACACCGCTGTCTTAGCGTACTATACCCTTGAAAGAGCAATTGGGGAAGAATTACCTCCTTTCTCGCCCCAATATGGAGCCCGCGACAGCTCTTCCCACAATGTGAGTGTCGGGGGCTAATATAGTGTAGTATCCCGCCCTATTCCCCCGAGAACTGAGCCACACTCCCTAGATGGTATAGCGCCCCTCGAGGGTCCTCGATATTATTATTGCTCCGAATAAATATATCAGAATATCGAAGGACTTGGGTCTGTCTTTTATCCGAGGCGABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-01-26 15:12:18 -05:00AEPt;AEPt;APFN ZTAPXV2APrN 5SAPrV1APrX}}5\ARTNASPFASPtDASPtDB1PtCB1PtCBCTS]BufTy O-CMNT 5CTIDOCTNMO CTOwOCTTL 5 CpEPDATA@DATA@DATA@DATA@DATAy DATAy DATAy rDATAy )dDATA =FzZ]DATA =FzDATA =FzOuDATA =FzDCHTDSamDySN 4Dye#DyeN4DyeN4DyeN5DyeN5DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 4EVNT 4EVNT4EVNT4FWO_GATCGTyp!!4HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~\bLIMS!!5"LNTD2LsrPaMCHNMODF5CMODL3730NAVGNLNE`NOISDP1AM>|Q4P1RL>|NP1WD>|SP2AM>|XP2BA>>[$P2RL>|V,PBAS>>FPBAS>>DPCON>>MzPCON>>L<PDMFZ=PDMFDPLOC>|IPLOC>|GDPSZE`PTYPO%PXLBRGNmNRMXV1RMdNFRMdV1RMdXFRPrNNRPrV1RUND RUND RUND RUND RUNT  -RUNT  2RUNT  /#RUNT  7Rate 4RunN""NS/N%DSCAN@SMED SMLtSMPLZSPACMSPACDSPACMSVER4.0SVER ]SVER,,4VScal@Scan@TUBEC3Tmpr<UserphARphCHDphDYbigphQLcphTRphTR;This is the confidence range *ABI_Limits..FVocAEPt;AEPt;APFN ZTAPXV2APrN 5SAPrV1APrX}}5\ARTNASPFASPtDASPtDB1PtCB1PtCBCTS]BufTy O-CMNT 5CTIDOCTNMO CTOwOCTTL 5 CpEPDATA@DATA@DATA@DATA@DATAy DATAy DATAy rDATAy )dDATA =FzZ]DATA =FzDATA =FzOuDATA =FzDCHTDSamDySN 4Dye#DyeN4DyeN4DyeN5DyeN5DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 4EVNT 4EVNT4EVNT4FTablFFVocl5FWO_GATCFeat//lGTyp!!4HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~\bLIMS!!5"LNTD2LsrPaMCHNMODF5CMODL3730NAVGNLNE`NOISDP1AM>|Q4P1RL>|NP1WD>|SP2AM>|XP2BA>>[$P2RL>|V,PBAS>>FPBAS>>DPCON>>MzPCON>>L<PDMFZ=PDMFDPLOC>|IPLOC>|GDPSZE`PTYPO%PXLBRGNmNRMXV1RMdNFRMdV1RMdXFRPrNNRPrV1RUND RUND RUND RUND RUNT  -RUNT  2RUNT  /#RUNT  7Rate 4RunN""NS/N%DSCAN@SMED SMLtSMPLZSPACMSPACDSPACMSVER4.0SVER ]SVER,,4VScal@Scan@TUBEC3Tmpr<UserphARphCHDphDYbigphQLcphTRphTR-&/Ur1nsjEth@t#Rt+7+:W~TW<=?-O?;T9"MYMv'nE-\jC}RjJgi8fDtrWT 75Hya0#C"4kyE7*r;IYLDK2N<@O(LxC[>({]>R/Sao&w%:T?4ze^kQtAN-:d=iU~Hfzq?$y!TWrK96``^,Ffn@v-Gt>iHAj}W2yv!a!<}B]rQHfKbQS&sF{LbzMiW~!Tq*Yh-8Z0s&^_XUQLzIQ0%}jx7Z]eth'=o_(@=8Ab;_?{-2Rmci0D8}QhyW\j&1?I| 9I+VWx;#2ps'Mv.h.9q8!0b) e:=Kna9W&a:Oc1o>Zz%8+!3@*7_62n`4573c5?8(F}z2g[jWcg;Z6Obe1Cd}Fp82)XaM~6>udrV|gwCiOfFc':Z^j?F?hECel7B]%j'kjWceOSx+C!K}Z'BNp{ e,&o.jrm$v5GK:.\_'[wA#Q9Tc T-*<^k'nwi5'S;.xhKn6g;X6YMI){wclQ[>A{%i/"a-cZ,f*F8B,}|O[nY?KPQh3f9bz14r1={oTWIn@.Nsz)}z)})i]$!@_|,? W]H6X+9mvH%lFU?-4$&FkNp&iJ-[qr[&Xj|82Z~9}j~:HzAb9ECQ/}Z&['K2;+hj3ST?0sKG'BNp3JWTX+9_e VO[O~}4}XfSap<#2_<2R57}?hik?]wJUo6X?/WZ#&a:Oc!X|eDNJ{s3x>rb TQ3U'.QR0Iel1}ssb[l"G>=?]p&qqQIxw}H{-*zir8rP p'Ic62nFT16Y=*"|Q4P1RL>|NP1WD>|SP2AM>|XP2BA>>[$P2RL>|V,PBAS>>FPBAS>>DPCON>>MzPCON>>L<PDMFZ=PDMFDPLOC>|IPLOC>|GDPSZE`PTYPO%PXLBRGNmNRMXV1RMdNFRMdV1RMdXFRPrNNRPrV1RUND RUND RUND RUND RUNT  -RUNT  2RUNT  /#RUNT  7Rate 4RunN""NS/N%DSCAN@SMED SMLtSMPLZSPACMSPACDSPACMSVER4.0SVER ]SVER,,4VScal@Scan@TUBEC3Tmpr<UserphARphCHDphDYbigphQLcphTRphTR