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RRLLLkkk%%%///888AAAJJJRRR[[Zcccjjjrrryy}&&'''''(((())))****++++,,,,----....///000001111222233334444555556666677777888899999999::::::::::;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 7a45e5660a5f45caad51a00bffd5bec3Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2017-01-26_13-12_16533130Seq18007137718_K1319_psbajmadera96-Well%K1319_D2_Pseudognaphalium_psbaf_psbafKB_3730_POP7_BDTv3.mobSeqPop7z  !"#%''%" ##!#-7>@>8.$ ,6>@<3)&,//,(%&+28;6, ,682'  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"5K`ntn_I1                             (4BP[a`YL=1*,6EWdjeVA+         "*/1112330*              &3;><6-#                    !!                 #$!             !#!           %+//,& !"!  !$(**)''*07<=:4,%     #&*.4;CKPPLE>843320,)%#!"'.5;=:3*"                                                                                                                                                                                                                                                                                                                                                             $&$                                                   {@A&sAKAoKB.bcpKB_3730_POP7_BDTv3.mobtermTCCCATTCTTCTAAACTTAGCTGCTATTGAGCTCCATCTACAAATGGATAAGACTTTGGTCTAATTGTATAGGAGTTTTTGAACTAAAAAAGGAGCAATAGCGTCCCTCTGGATAAAACAAGAGGGCATTATTGCTCCTTTTTTTATTTACTTACAAAATTTCTTTCAAAATTATTTGGTTGGATTCGCGTATTTTCTCTTTGTATTTTTCATTTATATATTAAATTTTAAATATTAATTGAGGTTTCTATTCTATTTCTATTCTATCTATTCTATATCCTTTTCTTAATCTTTTAAGAAGTTTTATTTCTAATTCAATTTCAATCGAAAATAAAATAGAGAAAAATTAAAATTTTGCTTATTTATTACTTTTATTTATTTTTATTTTATATTAGAATTTTATTTTATAAATAAGAACCATTTATTTATTTTATTTTTTTTTCTTTTATAATTATTATTATATTATTCTTATTATATTTGTATGTGTGATAATTTGAAATAAGAAATAAGAACTAGAAAATAGTAGAAGGGGCGGATGTAGCCAAGTGGATCAAGGCAGGGATTGTGGAATCCGCCATACGCGAAGGGAAGTTTCCCATTCTTCTAAACTTAGCTGCTATTGAGCTCCATCTACAAATGGATAAGACTTTGGTCTAATTGTATAGGAGTTTTTGAACTAAAAAAGGAGCAATAGCGTCCCTCTGGATAAAACAAGAGGGCATTATTGCTCCTTTTTTTATTTACTTACAAAATTTCTTTCAAAATTATTTGGTTGGATTCGCGTATTTTCTCTTTGTATTTTTCATTTATATATTAAATTTTAAATATTAATTGAGGTTTCTATTCTATTTCTATTCTATCTATTCTATATCCTTTTCTTAATCTTTTAAGAAGTTTTATTTCTAATTCAATTTCAATCGAAAATAAAATAGAGAAAAATTAAAATTTTGCTTATTTATTACTTTTATTTATTTTTATTTTATATTAGAATTTTATTTTATAAATAAGAACCATTTATTTATTTTATTTTTTTTTCTTTTATAATTATTATTATATTATTCTTATTATATTTGTATGTGTGATAATTTGAAATAAGAAATAAGAACTAGAAAATAGTAGAAGGGGCGGATGTAGCCAAGTGGATCAAGGCAGGGATTGTGGAATCCGCCATACGCGAAGGGAAGTT!(:DK`fnw(6DWgs~#19EP]kt #/8DQ_mx'2>JWbny".;EOZep{ ".:GS^kx%/;GS^jw(4BNZfs    ' 2 ? 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GW%cc`KHI`Y3:.PG2EDSJG[4'E'd#b26Q5SBU0GU;LA%;"GM2XcG2W4'U"F")=+A2D1B;D&]12<< #%]H7O2%,PG.FB23YO_K ]A+;]G'mTKGEh j3:Qr +`wec|E\$+7-hY pKo\ qP{?Q l*=kvf8 Sd~ v_;'Axl6}(IvmXym!F@l / IT(bN Fp7 CJ8 /cG\8it<A6 P]x_4T9zN#=`): D,gK0h7`=G ^k4c&4A6Tu%_XVr*G1h!U0!~M ;Gz9(# 3Z:#[:Ue _ N#I}z6uUE$JiF#0~DwE(| T@nYFim6 Tw1v*U%b0w'fKO,-}sS;mt~eL# &_*D|H)E};Uu#elh} Dc+l6>}rbhUgaR^Hy^VF5:#>F>'<Sq*4D3U,:51#B)8iC%+B*8 ',*!*:!  (#E* 4!    &  0 %    &(3."L 5  * A#0 0 %  H!A,?%<P!5I'%<<K8j`)03 # Y\*'Mf IU#D6c=e)$ E(9F(S/& $-Ic/2dG3a![=&1%!'%>$|),UPE6O-*(E5pdc>(JdP1sq+5` @A44okheO|\9999+W++$%+B:+91+;+YO 99+99>+999 9E'#l/'l =kv?Vf5(K!Xllllpap{pp9Oe)QE I7m$p p0ixp{1iXs,=yt084Gr;%S{4I\D =edpO.8:oRpRR ch<(ep zpEI7GHa c'rpmH uN0]L@iVeP3* #%()(K +]YNV+5 Z6i|_3,j-sUcP"{!T-=1B)X;Ra8&[=.hkNIp;?8n&;O6[CMFAVzc}Tjou\vV+_5NtzhDww >|gb 3)b? TQ%/L.9=>1<7$Br]m9D8@oO*gr%<_;DLM,BgI2V/>EQ ;LAm R uQ69nE#`TRU!gNhG%g6rVH CK |cs?9 X q'G3~(EIx'~Y .<#-``V,)*h^pwuAAAATACTCCTCTGCACATATGTGATAGAGAGCTACATACACGTACATATGACACCGTTGTATTAGTGTATAACCCCCCAAGGGGGGGGGGTAGAACTAGATTCTTTCTGTTTATTCCTCGAGAAAAGCAGAGCGCATCAAGAAATGAACTCACACGGTAACTGCAAGGCTAATAAGCTGGAAGGCGCTCATCCCCACAAAAAGGAAAGCTCACATATATAATTTAAAAGGGGGGGCCAATTTAAACTCGACTATACCAGTCCAAGGGCCGGTCCATAAAAACGAAGGTGGGGAATTTCTAAAGCCAACTCCCACGGTAAAAGGAGATTTTGTCCTATTTTCCTTTGGTTTTCCCGTTGAGCAAGCAGGAGAATAAGGACAAAGACGGCAGGCGAGGAAGCTAACAGAGGGGGGAGCAACAAGTACCGGGAATAAAGAAGAGGGGCGGAGGAGGCCCAGTGGAGCGGGGCGCGGGCAGGAGTGTCAGCCAGGCCAACCCAGCCCCTCTCTTGGATTTTTTCTTGTTTGTTACTTTTATGGCGGTTCGCACAACTATGGTAATGGTGATGTATACTATCGAAACCGCAATGACGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-01-26 15:12:30 -05:00AEPt=3AEPt=3APFN Z\APXV2APrN 5SAPrV1APrX}}5\ARTNASPFASPtLASPtLB1PtKB1PtKBCTSyBufTy O-CMNT 5CTIDOCTNMO CTOwOCTTL 5 CpEPDATA@DATA@DATA@DATA@DATAy DATAy DATAy rDATAy )dDATA >6|lZeDATA >6|lDATA >6|lS=DATA >6|lϩDCHTDSamDySN 4Dye#DyeN4DyeN4DyeN5DyeN5DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 4EVNT 4EVNT4EVNT4FWO_GATCGTyp!!4HCFGCEHCFGHCFGHCFG==InScInVtLANE.LAST~~xSLIMS!!5"LNTD2LsrPaMCHNMODF5CMODL3730NAVGNLNE`NOISLP1AMQczP1RLQ^P1WDQhP2AMQq`P2BAQQvP2RLQlPBASQQNPBASQQLPPCONQQ\PCONQQZ6PDMFZEPDMFL4PLOCQUPLOCQPPSZE`PTYPO%PXLBRGNmNRMXV1RMdNFRMdV1RMdXFRPrNNRPrV1RUND RUND RUND RUND RUNT  -RUNT  2RUNT  /#RUNT  7Rate 4RunN""NS/N%LSCAN@SMED SMLtSMPL&&ZSPACA_ϝSPACL-SPACA_ϝSVER4.0SVER ySVER,,4VScal@Scan@TUBEB6Tmpr<UserphARphCHLKphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt=3AEPt=3APFN Z\APXV2APrN 5SAPrV1APrX}}5\ARTNASPFASPtLASPtLB1PtKB1PtKBCTSyBufTy O-CMNT 5CTIDOCTNMO CTOwOCTTL 5 CpEPDATA@DATA@DATA@DATA@DATAy DATAy DATAy rDATAy )dDATA >6|lZeDATA >6|lDATA >6|lS=DATA >6|lϩDCHTDSamDySN 4Dye#DyeN4DyeN4DyeN5DyeN5DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 4EVNT 4EVNT4EVNT4FTab7FVoc&FWO_GATCFeat//GTyp!!4HCFGCEHCFGHCFGHCFG==InScInVtLANE.LAST~~xSLIMS!!5"LNTD2LsrPaMCHNMODF5CMODL3730NAVGNLNE`NOISLP1AMQczP1RLQ^P1WDQhP2AMQq`P2BAQQvP2RLQlPBASQQNPBASQQLPPCONQQ\PCONQQZ6PDMFZEPDMFL4PLOCQUPLOCQPPSZE`PTYPO%PXLBRGNmNRMXV1RMdNFRMdV1RMdXFRPrNNRPrV1RUND RUND RUND RUND RUNT  -RUNT  2RUNT  /#RUNT  7Rate 4RunN""NS/N%LSCAN@SMED SMLtSMPL&&ZSPACA_ϝSPACL-SPACA_ϝSVER4.0SVER ySVER,,4VScal@Scan@TUBEB6Tmpr<UserphARphCHLKphDYbigphQLcphTRphTRR)U`l5j!X`7.qT*uq&6i}8Amabj1A6rd)ziA=sUOzj{>ZHm? V<(?:13Uv|Q{9V \+Yoe2{sGi=&u@B\$nq%StumfXr9lKnD8Y~Csk%pM_T![sGj`X0?888g2lI>iV=1SbN-&e}hw@q}%Q`+N@c'WQwK m}{H1}z3/onxd:}ml]q:jJRWO[@*!$T]_`G]p-@Qhiq|fq'#kD:_f7o,f9r;qBwJUo6;^UWZi7WeOzJu%~4%rIZHp#)+KT>:L-Ev!$V@AKl&O`x Pz5's1Hhl@tt/kp<=3#RF@4$+KV=[H7Cpt`3AC?-4$&#S%TwKy3ofmq*6Y?Qs?\g>?{:LKu292CB|V&Xc|LVn!<|'@4o*MK:.<)%,8#V>Ax`X~lTmt.qK|-o*.AQ>UA!%EeKOWQ`QV-!w2l89+uTE#!#hmmfc4hAo?e3{0+oiw87[%Vr6R[Ule,rS]RnjH,3LiPtj glX ,cW`}F)h>?[ha_DMOFU>^[?|&Rv3672Z)~6bI.J:*yEwiOM#Y\V&&OE6OL}IlE]&l8EE.Om,\eyDWYI!8U-o9D4ogXY@ZB 3O{ ~VJ(/on}sF{AR8=(V-OGC#!^MrrG]fQ/LBUYe:?Gvy3r^6j4G4#Ml(8wDP'f t1/o8/t$1|5O-U[d>7:I128)TK7<4Zm[E,fcd]R03DiU3o XSXW .CTNCwD(ftyYZFU5|8e7spFp[O6h"4#O|!lYqdqY7_twvr]LaiY2]2r-U[D~TWrSNmWT/@{K m}{H1}zIeD8zEXTM~ykI0o6Gzv&1NmbcnNl_a?FQPU{mwY1XV7:>JWT|;B9t-yc=3E;qT%;5]U'C,CsDa)xz4|Md?]R_s_beB Ri/"@6|lZeDATA >6|lDATA >6|lS=DATA >6|lϩDCHTDSamDySN 4Dye#DyeN4DyeN4DyeN5DyeN5DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 4EVNT 4EVNT4EVNT4FTab7FVoc&FWO_GATCFeat//GTyp!!4HCFGCEHCFGHCFGHCFG==InScInVtLANE.LAST~~xSLIMS!!5"LNTD2LsrPaMCHNMODF5CMODL3730NAVGNLNE`NOISLP1AMQczP1RLQ^P1WDQhP2AMQq`P2BAQQvP2RLQlPBASQQNPBASQQLPPCONQQ\PCONQQZ6PDMFZEPDMFL4PLOCQUPLOCQPPSZE`PTYPO%PXLBRGNmNRMXV1RMdNFRMdV1RMdXFRPrNNRPrV1RUND RUND RUND RUND RUNT  -RUNT  2RUNT  /#RUNT  7Rate 4RunN""NS/N%LSCAN@SMED SMLtSMPL&&ZSPACA_ϝSPACL-SPACA_ϝSVER4.0SVER ySVER,,4VScal@Scan@TUBEB6Tmpr<UserphARphCHLKphDYbigphQLcphTRphTR