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(<,,,III^^^{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR* RRLLLkkk%%%///888AAAJJJRRR[[Zcccjjjrrryy}&&'''''(((())))****++++,,,,----....///000001111222233334444555556666677777888899999999::::::::::;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 7700835589d54301be75c8146565ac52Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2017-01-26_13-12_16533130Seq18007137718_K1319_psbajmadera96-WellK1319_C5_Lantana_psbaf_psbafKB_3730_POP7_BDTv3.mobSeqPop7z+)% "%# $(+-.-,*)('&&%&(+/13210//.+'" %,//+%!(1:?@=81*$   '2?KVbo/BWnpZB)vk_QB5*$!   !'0:DNZh{9Qi~u]@ tW<%  "%(+08DUn>B<|mZ>eH2       -@\DqyCqQ8%           "%''%$"#*<a; x\*ieM >DLIN)      /EbJ&PpD)   ,_h WhOTU(&K}FMU5% ""!=yw;+>>Yd^     <~>=S>B+-ic o?! 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"!%"  -$* 2 %   "& ' )34.&6&=3'G\?$M&jX yV~CAACTATCCTAACAATATGTGATAGATATCAACACGTATATATGACATCGCCTCAGAGTATAGAAGTGCACCCTTTTAGAGGGGTTATATTTCTATGCAGCGGTATTCTCTCCCTCGTGCAATAGCACGAGAAAATGATAGCGCTTCAATAAACTCCCCACGGACGCCTCTAAAGCAAGTAGAAGAGTGTTCCGAAAGAGGACCCTCGAAACTATAGCTCAGGGGGGAGGGACGGGAAAGACTCATTGACGGAGAACGAGGAGAGATTAACAAACCCTCACGGCGGGAGGAAAAGGTAAAACGACTTCCCACCGAGGTACACTTACACCCGCATAAAGCTAGGTAAATGGGTTAGGTGTATGCAATGCGTTCATGCAAAGCAAAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-01-26 15:12:57 -05:00AEPt@.AEPt@.APFN ZSAPXV2APrN 5SAPrV1APrX}}5\ARTNASPFASPtASPtB1PtB1PtBCTSp BufTy O-CMNT 5CTIDOCTNMO CTOwOCTTL 5 CpEPDATA@DATA@DATA@DATA@DATAy DATAy DATAy rDATAy )dDATA >}Z\DATA >}2DATA >}VDATA >}DCHTDSamDySN 4Dye#DyeN4DyeN4DyeN5DyeN5DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 4EVNT 4EVNT4EVNT4FWO_GATCGTyp!!4HCFGCEHCFGHCFGHCFG==InScInVtLANE_LAST~~nLIMS!!5"LNTD2LsrPaMCHNMODF5CMODL3730NAVGNLNE`NOISQP1AM`P1RL]P1WDcP2AMiP2BAmP2RLfPBASSpPBASQPCON\vPCONZPDMFZ<PDMFQPLOCWPLOCTPSZE`PTYPO%PXLBRGNmNRMXV1RMdNFRMdV1RMdXFRPrNNRPrV1RUND RUND RUND RUND RUNT  -RUNT  2RUNT  /#RUNT  7Rate 4RunN""NS/N%QSCAN@SMED SMLtSMPLZSPACAiJSPACQSPACAiJSVER4.0SVER pSVER,,4VScal@Scan@TUBEA11Tmpr<UserphARphCHQphDYbigphQLcphTRphTR{This is the confidence range *ABI_Limits..FVocAEPt@.AEPt@.APFN ZSAPXV2APrN 5SAPrV1APrX}}5\ARTNASPFASPtASPtB1PtB1PtBCTSp BufTy O-CMNT 5CTIDOCTNMO CTOwOCTTL 5 CpEPDATA@DATA@DATA@DATA@DATAy DATAy DATAy rDATAy )dDATA >}Z\DATA >}2DATA >}VDATA >}DCHTDSamDySN 4Dye#DyeN4DyeN4DyeN5DyeN5DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 4EVNT 4EVNT4EVNT4FTab~fFVoc~UFWO_GATCFeat//~&GTyp!!4HCFGCEHCFGHCFGHCFG==InScInVtLANE_LAST~~nLIMS!!5"LNTD2LsrPaMCHNMODF5CMODL3730NAVGNLNE`NOISQP1AM`P1RL]P1WDcP2AMiP2BAmP2RLfPBASSpPBASQPCON\vPCONZPDMFZ<PDMFQPLOCWPLOCTPSZE`PTYPO%PXLBRGNmNRMXV1RMdNFRMdV1RMdXFRPrNNRPrV1RUND RUND RUND RUND RUNT  -RUNT  2RUNT  /#RUNT  7Rate 4RunN""NS/N%QSCAN@SMED SMLtSMPLZSPACAiJSPACQSPACAiJSVER4.0SVER pSVER,,4VScal@Scan@TUBEA11Tmpr<UserphARphCHQphDYbigphQLcphTRphTRc( !j rh[Z66YM'KvT+F4:H[);@#PA+eg[gUGGN*+j:xUIc.jCxO{VZHngM0xW#nZE35ux2]DM6mtTUk1T:29"5-z]T1S6RA0W7yLlo/&U{uclQ[vL}W$P1ObYn#pwW(Y`N!:X>zt %v1${NW8:7F}wxSB#_{ykI0ouNbv&~^\mcJTGyr{[Kl_e5 }37RIBWjt(mID9`ht1A'obU2kR@ef&e}hw@q}%Q`ALIghJ*(k;:|e}OM *R@_>j23T6Y/Xh]'p8Npdo(ktnP_I[Dd@2g|5J_@A[]"p64y^M_9Ui|2Z/}NVE]SwS'8X|U\g?v!YH}&8)TK72K`rxPw% e M]Pz%y;|>EYWC.%o,n`!39G,RS_%ssaP8/GC.JuCglxsQlnWH%`WZ\9~#\W~XF 4S]"~zI#Ml(8wDP'f t1Y*6/t$1|5O-U[d>7:I128)TK7<4Zm[E,fcd]R03DiU3o XSXW .CTNCwD(ftyYZFU5|8e7spFp[O6h"4#O|!lYqdqY7_twvr]LaiY2]2r-U[D~TWrSNmWT/@{K m}{H1}zIeD8zEXTM~ykI0o6Gzv&1NmbcnNl_a?FQPU{mwY1XV7:>JWT|;B9t-yc=3E;qT%;5]U'C,CsDa)xz4|Md?]wclQ[>A{%i/"E.Om,\eyDW"[F%.eq$(Rj:j]NgJ)WyU~Rl]hl5cS_d.u?zAw3+fF<7:cFU.F4/~L][U_1(_CcS{[}7"=I).zP{?7.N{Cj_"@?j~J!Eb4;,}E -kb+N4+t457fc|+{Wj2oFtX>j3XV]'p8NpdNmbcn3?GY\U {%KTfx'H9Kx'!byXYhRwZj1& evr.FZH~1~WGUcN]`VFX+W@-L$"Ohv]n&19!O3}RSVSFsA b':8 v/gg61C8dk7YZFU5|8e7s.FSKpuzeqr[&Xh|ZC!aV"OMb=KubQ2a5|OjAZeXVm? >S-4L7=xU;C,6KP8)s5AVihwYc_`]:L]{RO7K57:=&CaF-B]%jyeY5+!hs|*>H~?p?}@8jaU(]y"/:Boz?xNu~)B*#qc3D44[_o5qS+SP7T]^q;,`h?&03S}Z\DATA >}2DATA >}VDATA >}DCHTDSamDySN 4Dye#DyeN4DyeN4DyeN5DyeN5DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 4EVNT 4EVNT4EVNT4FTab~fFVoc~UFWO_GATCFeat//~&GTyp!!4HCFGCEHCFGHCFGHCFG==InScInVtLANE_LAST~~nLIMS!!5"LNTD2LsrPaMCHNMODF5CMODL3730NAVGNLNE`NOISQP1AM`P1RL]P1WDcP2AMiP2BAmP2RLfPBASSpPBASQPCON\vPCONZPDMFZ<PDMFQPLOCWPLOCTPSZE`PTYPO%PXLBRGNmNRMXV1RMdNFRMdV1RMdXFRPrNNRPrV1RUND RUND RUND RUND RUNT  -RUNT  2RUNT  /#RUNT  7Rate 4RunN""NS/N%QSCAN@SMED SMLtSMPLZSPACAiJSPACQSPACAiJSVER4.0SVER pSVER,,4VScal@Scan@TUBEA11Tmpr<UserphARphCHQphDYbigphQLcphTRphTR