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RRLLLkkk%%%///888AAAJJJRRR[[Zcccjjjrrryy}&&'''''(((())))****++++,,,,----....///000001111222233334444555556666677777888899999999::::::::::;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 824def16afef4fcfbc377cab299ba8f4Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2017-01-26_13-12_16533130Seq18007137718_K1319_psbajmadera96-Well K1319_C11_Passiflora_psbaf_psbafKB_3730_POP7_BDTv3.mobSeqPop7z!! 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B1x>    $&#    #fF(^V0c+         "`9 ^a)Ef$           =k!=v6          (ahu}gadO      .`'PZFb9     $&%      +7AGGA7*   ;jiryl5 iS=8644@aS C]U0c#Su;~0kWF8.)*4Gc|bOGJUbnql`SP^y- =eKFO_pH0wZ9mfhnv~zl[J:.% &6I[hnlcUD3#                                                                                                                                                                                              U@]dAANAKB.bcpKB_3730_POP7_BDTv3.mobtermACCCTCCTCTGGACTAGCTGCTTTTGAGCTCGATCTACAAATGGGTAAGACTTTGGTCTTAGTGTATACAAGACCTTGAAATAAAGGAGCAATAACCAATTTCTTGTTATATCAAGGGTATTGTTATTGCTCCTTTATTTTTCTTCCATTTTTTTATTTACTACTTTTTTTTAGCCTTTTTTTAAATATTAAAAAAATGAATTTTAAAAAATTCATAAAGGCTATTTCTTCTTTTCTTTCCATTTTTTCAAAAATGGAAATTCTGCTAATATTTCGGTAGTTTTAAACTAGAGTATTGGGGCGGATGTAGCCAAGTGGATAAAGGCAGTGGATTGTGAATCCACCATGAAAACGCGAACCCTCCTCTGGACTAGCTGCTTTTGAGCTCGATCTACAAATGGGTAAGACTTTGGTCTTAGTGTATACAAGACCTTGAAATAAAGGAGCAATAACCAATTTCTTGTTATATCAAGGGTATTGTTATTGCTCCTTTATTTTTCTTCCATTTTTTTATTTACTACTTTTTTTTAGCCTTTTTTTAAATATTAAAAAAATGAATTTTAAAAAATTCATAAAGGCTATTTCTTCTTTTCTTTCCATTTTTTCAAAAATGGAAATTCTGCTAATATTTCGGTAGTTTTAAACTAGAGTATTGGGGCGGATGTAGCCAAGTGGATAAAGGCAGTGGATTGTGAATCCACCATGAAAACGCGA*8HOVez'4BRalw *5BLXenz(1=GQ\jx(3?IT_jv&0;HR]jv)5AMZeo{,7ALYeoz ".;GT`lx   $ / < H T _ l x    ' 3 @ L X d o {  # . 8 D P ] i u  # / ; H T a k w    + 6 A M X e p } *4@LXcp| %1=IUcpz !,5?KWbm{*8HOVez'4BRalw *5BLXenz(1=GQ\jx(3?IT_jv&0;HR]jv)5AMZeo{,7ALYeoz ".;GT`lx   $ / < H T _ l x    ' 3 @ L X d o {  # . 8 D P ] i u  # / ; H T a k w    + 6 A M X e p } *4@LXcp| %1=IUcpz !,5?KWbm{   %11,:'545*+'!/>!>>/86>8/>>>>/8>>>>>>;3+;>>+>;;;>>>;6>>;>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>;>>>>>>;;>>>>>;>>>>>>>>>>;>>>>>>>>;;>>>>6>>>>>>>>;>>>>>>>>>>;>>>>>>>>>6>>>>>>>>>>>>;>6111>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>;>>>>>>>>>>;>>>>>;>>>>>>>>>>>>>>>>>>>>>>>8>>>>>>>>>>>>>18>>>>>>>>8>8//8 88>8444>.&6>>&/.(    %11,:'545*+'!/>!>>/86>8/>>>>/8>>>>>>;3+;>>+>;;;>>>;6>>;>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>;>>>>>>;;>>>>>;>>>>>>>>>>;>>>>>>>>;;>>>>6>>>>>>>>;>>>>>>>>>>;>>>>>>>>>6>>>>>>>>>>>>;>6111>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>;>>>>>>>>>>;>>>>>;>>>>>>>>>>>>>>>>>>>>>>>8>>>>>>>>>>>>>18>>>>>>>>8>8//8 88>8444>.&6>>&/.( PJ.B' D:@5 <Q;8\/JN-FH])\b*UYDV& !!GM:2G\$S] _V("1:/6X!IN $2Xb W=HD=[; J0'%,*AI LEW$WD&G P3P) E3S4!b< 5Z&)>>0,KGS3b:XYERR#NH)Z`6FEP;(KGR0I 60!EJ=] X7/%EP2C*KJc+"[C/AZS81 *V2M'=DSX[^cH:+_ $"D0"EE#B6%@4Z8*Y9-=!5C%5L DM9:)N&8X$W7R1=(a&=^*XEW5@%F?6?D7 33#2T PE`0U%?S+.ZY 8XFH?42g]3 P/<$hLm}M <@XD${](uFU}I/\]> 9~X=#y9OnQ48 *k%aw`:%x|[  8i\ icme#\$i!}sE;LfE"$&z|~^6=:`V yW-[@$s9_zxv5c ll-`HFCFP 8EVKd- 4Yg c. P; 3u1c&%A[||am^xZohkQliBE>-,M9<H.0D)<.)(+ A)-"%!L'5  &2                   '*  8a8pppp!L!, 9p>d>75Pp|?p UNZSmdpd,o;(#pRxk*P!/SKp>d<VDS6AIyud z0dx'D?H ,I4FD,;daI88efp"3']s^2#B,u `F_ja[Aw 3t Cd-*415F-41Al<.M [(U*,D%'.s&67()! & /#61-&(#"&+0@% ,#, 1#I  &*$9 $ %, '+(!Ru''  (!&  &*  !"##O0 '!,&''",)  2 $L1. !#&AD,&,''33<((.RPfd=F//O_*Q4p3 +CAAAATTCTAAACAATATGTGCAAGAGAGCTCTATACACGTACTTGGGACACCGCTGTCAGTGTGTACACGACATCGAGGTAGGGAAGCGTTATTAACTCCCTCGAGATATCACCCAAATAGAGGTAGCGCTTCAATAAAACGGCTACGGGAAAATAAACGACTCAGGACAAGCGTCGCAGAATGTGGGGTTCCCGTAAGTGCCATTTGTTACTTCCTGACGAGACCTCCTCCCCTCCCTTTAAAAACACGGCGGAAGGTACTGCGACCATAACTCCGGCGCAATTCTAGAGAATACCCACGAATATATCGGCTCAAAGGGGGAATTAGAAGAATACGGCTACTGCCCCCGCGAGAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-01-26 15:12:22 -05:00AEPt<AEPt<APFN ZWAPXV2APrN 5SAPrV1APrX}}5\ARTNASPFASPtHASPtHB1PtGB1PtGBCTSsBufTy O-CMNT 5CTIDOCTNMO CTOwOCTTL 5 CpEPDATA@DATA@DATA@DATA@DATAy DATAy DATAy rDATAy )dDATA ?TZ`DATA ?TٴDATA ?TYDATA ?T\DCHTDSamDySN 4Dye#DyeN4DyeN4DyeN5DyeN5DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 4EVNT 4EVNT4EVNT4FWO_GATCGTyp!!4HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~rjLIMS!!5"LNTD2LsrPaMCHNMODF5CMODL3730NAVGNLNE`NOISWP1AMeeP1RLecP1WDehP2AMen;P2BAeeqP2RLekqPBASeeYPPBASeeWPCONeeaPCONee`IPDMFZ@PDMFWPLOCe]PLOCeZPSZE`PTYPO%PXLBRGNmNRMXV1RMdNFRMdV1RMdXFRPrNNRPrV1RUND RUND RUND RUND RUNT  -RUNT  2RUNT  /#RUNT  7Rate 4RunN""NS/N%WSCAN@SMED SMLtSMPL!!ZSPACAo%aSPACWSPACAo%aSVER4.0SVER sSVER,,4VScal@Scan@TUBEB4Tmpr<UserphARphCHWphDYbigphQLcphTRphTR[This is the confidence range *ABI_Limits..FVocAEPt<AEPt<APFN ZWAPXV2APrN 5SAPrV1APrX}}5\ARTNASPFASPtHASPtHB1PtGB1PtGBCTSsBufTy O-CMNT 5CTIDOCTNMO CTOwOCTTL 5 CpEPDATA@DATA@DATA@DATA@DATAy DATAy DATAy rDATAy )dDATA ?TZ`DATA ?TٴDATA ?TYDATA ?T\DCHTDSamDySN 4Dye#DyeN4DyeN4DyeN5DyeN5DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 4EVNT 4EVNT4EVNT4FTabNFVoc=FWO_GATCFeat//GTyp!!4HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~rjLIMS!!5"LNTD2LsrPaMCHNMODF5CMODL3730NAVGNLNE`NOISWP1AMeeP1RLecP1WDehP2AMen;P2BAeeqP2RLekqPBASeeYPPBASeeWPCONeeaPCONee`IPDMFZ@PDMFWPLOCe]PLOCeZPSZE`PTYPO%PXLBRGNmNRMXV1RMdNFRMdV1RMdXFRPrNNRPrV1RUND RUND RUND RUND RUNT  -RUNT  2RUNT  /#RUNT  7Rate 4RunN""NS/N%WSCAN@SMED SMLtSMPL!!ZSPACAo%aSPACWSPACAo%aSVER4.0SVER sSVER,,4VScal@Scan@TUBEB4Tmpr<UserphARphCHWphDYbigphQLcphTRphTRK2OI*-V>yj6m}:)9`[?y[nvbeK]TM7l:2Fj62o.B3@dSOK[-z|G0_fs_c&a~4~kJF96YZ{sbV_f7o,f9r;qBwJUo6;^UWZi7WeOzJu%~4%rIZHp#)+KT>:L-Ev!$V@AKM\8}48vc. 7$?Z2o41{mXt@krT::_o5qSF1F7T3|2f[F6,6ir%u_bO?NL? nwt!}q|nc{[*M0JNbm|XMrDK"*.GHiJ(/\_'MChMoG;KMJ95nM,aVh6dYHp-Op;ZJLgS]jTj.uFk4W7|!y]18`'|_n?26.?bFWJQ:Ocl2[gLY'BO[T@{:AtRHiv<("Hf.QbU`AtG>udrV|gwCiOfF{\)lOYW|_nw|]jTj.uFPqWgZ*me2&dArXx6m}3,~~+N@c'v+gK >o>s1HT+O}L6>U{mwY1XV7:>JW*z(KC,Zy`[qDr_WG\|xT;#6RlSR5[h-er5!_uJUo6X?/WZ#3|5Dfqcd o;X.>Sxp/YgZir$G,f *"y&bJ~FT,{12.Wh?7kx`rx).fcEK$J39OQJM~EbrB%P^gW7~+lF{ryE^STt4)T(wfT;C,_,qz7.5k|_^Z0b>"EJX.=DaJ2`>nX$t!TQqvLWvq$\@U)tO83m^EG 9*q:bw\6I\(kluL\:KZI\T(k"T'o)6yHAj}=