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***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR* RRLLLkkk%%%///888AAAJJJRRR[[Zcccjjjrrryy}&&'''''(((())))****++++,,,,----....///000001111222233334444555556666677777888899999999::::::::::;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 55c6d01e7c104f2a93e4ce70f6c1aaefSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2017-01-26_13-12_16533130Seq18007137718_K1319_psbajmadera96-WellK1319_B9_Desmodium_psbaf_psbafKB_3730_POP7_BDTv3.mobSeqPop7z#'+./0/-,+*+,-./01479:73.,.3:@B@<768>EJLKHECCEFGEA:4/-,,+(""$!   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(./('-$# $* #)8$!%&&&>"-)8*3$14 &*;7G#)(#$, 4^#a5=MvT}d]m_]ZggAAAAATCTAATATATATGTGCTAGAGATCTACATATATGTACATATGTTACCCATCGCAGGGAGTACAAGTCCCCGAGGGAGGGGGAGCATTATCACCACTCTCGAGAGAGCTCCCCAACTGGGGAAAGTAGTGAACATATAGGAGGGTATACATCTATAGATAAAAAATGGAATGAGACTATAGAATACTCCAGGAAGATAGGTGGAGTATATATATATGCAGGCCTCTCTCTAAAAAACTTACACTAGAGGAGGGGATTGATATTGAGGGCCTAGAGTTCTAGATATAGATCGCGTTAACATAGAATATGAACGCATAAAGCTTCCTGTATGGGGCAAATCAATGGACTGTGTTCATGCCAACGGCCGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-01-26 15:12:39 -05:00AEPt>!AEPt>!APFN ZUAPXV2APrN 5SAPrV1APrX}}5\ARTNASPFASPt`ASPt`B1Pt_B1Pt_BCTSkBufTy O-CMNT 5CTIDOCTNMO CTOwOCTTL 5 CpEPDATA@DATA@DATA@DATA@DATAy DATAy DATAy rDATAy )dDATA >}Z^DATA >}`DATA >}TbDATA >}dDCHTDSamDySN 4Dye#DyeN4DyeN4DyeN5DyeN5DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 4EVNT 4EVNT4EVNT4FWO_GATCGTyp!!4HCFGCEHCFGHCFGHCFG==InScInVtLANE?LAST~~jLIMS!!5"LNTD2LsrPaMCHNMODF5CMODL3730NAVGNLNE`NOISNnP1AMr]P1RLrZ1P1WDr_P2AMreP2BArrhP2RLrbPBASrrPPBASrrNPCONrrXPCONrrWMPDMFZ>PDMFNPLOCrTiPLOCrQPSZE`PTYPO%PXLBRGNmNRMXV1RMdNFRMdV1RMdXFRPrNNRPrV1RUND RUND RUND RUND RUNT  -RUNT  2RUNT  /#RUNT  7Rate 4RunN""NS/N%NfSCAN@SMED SMLtSMPLZSPACAc)SPACN~SPACAc)SVER4.0SVER kSVER,,4VScal@Scan@TUBEA7Tmpr<UserphARphCHNphDYbigphQLcphTRphTRkThis is the confidence range *ABI_Limits..FVocAEPt>!AEPt>!APFN ZUAPXV2APrN 5SAPrV1APrX}}5\ARTNASPFASPt`ASPt`B1Pt_B1Pt_BCTSkBufTy O-CMNT 5CTIDOCTNMO CTOwOCTTL 5 CpEPDATA@DATA@DATA@DATA@DATAy DATAy DATAy rDATAy )dDATA >}Z^DATA >}`DATA >}TbDATA >}dDCHTDSamDySN 4Dye#DyeN4DyeN4DyeN5DyeN5DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 4EVNT 4EVNT4EVNT4FTabyFVocyFWO_GATCFeat//yGTyp!!4HCFGCEHCFGHCFGHCFG==InScInVtLANE?LAST~~jLIMS!!5"LNTD2LsrPaMCHNMODF5CMODL3730NAVGNLNE`NOISNnP1AMr]P1RLrZ1P1WDr_P2AMreP2BArrhP2RLrbPBASrrPPBASrrNPCONrrXPCONrrWMPDMFZ>PDMFNPLOCrTiPLOCrQPSZE`PTYPO%PXLBRGNmNRMXV1RMdNFRMdV1RMdXFRPrNNRPrV1RUND RUND RUND RUND RUNT  -RUNT  2RUNT  /#RUNT  7Rate 4RunN""NS/N%NfSCAN@SMED SMLtSMPLZSPACAc)SPACN~SPACAc)SVER4.0SVER kSVER,,4VScal@Scan@TUBEA7Tmpr<UserphARphCHNphDYbigphQLcphTRphTRf\mci2a>qN8>o Ueh,9n5g~Ri6(*24;x$}FNVUCs-113xT]o7U"y6^i_y>]y&m"o`^ kS\KQpYp&!gL-GI&7.{Mco6m~2HBIUbe1Cd}FxQZEZ,}4HUXYnuPQhoNePv7b{Wj2oFtXyy3@=?]p&)C^DZ=CHTA,h{|dsYuTF}wK[*oCfo;LY&p5S_3I|o6HvFHZ( g>}WqD@~TlW+b@cT]`AqhjYm2N^d,*o66yQo].5b"w\O_y1m-(-hkdr&1ZG|JWm;2FVc.O_l;C[;6&`_M%_|_n?_;?L-&]S9Hgn_RJ4ir$G%1` b&}3672tX.z=@e?xpL#H~?p M;_8Es{sMV^@}OPDsvA\Fu*tnPl'Z9dgTiK]5bf+mbB9YKAmN}9LyHj@C W4ov.lN"@>oW~!Te4;,}aD[h_R9wQi:A,b?Az{fzVS:3AG}X`AL:2ZE?RVuUvcPkga]FU-`Vn!<|'84?73z<^*5 %6pebvM<$AJT[Mo"$+r:y`xH/whA<%X#Ml(8wDu`D$AhVKubQ2a5NDzU4oFo;X.u8xXIc62nrD5pY(d+{o.jaFsA bXX<,x'EEwiOfF{\)lOY1|YL0eF`x&iJ-A|K7~57|NxOrz6 ?63_iPQ2aLtyc@"SD=;LX@HgX63g4`m^*Q1V'.$b[2$YWmOyl}$rEGCN8V+lwc7*<&YTIu{Qqk+^tpu"pcSIoM/V/5`agvLAX;:,aXj`Q Dx6m}aD&Wr`sEHu]4kkSnwkI6 +FwMz%FT!sz^`A(O>|ESRDujXTE-yh;}S$oa$N;172e,qz7|!f|bEdG[~{?26ttRHSK%}jx7Z]et|?%9'o *&AEPt>!AEPt>!APFN ZUAPXV2APrN 5SAPrV1APrX}}5\ARTNASPFASPt`ASPt`AUDTB1Pt_B1Pt_BCTSkBufTy O-CMNT 5CTIDOCTNMO CTOwOCTTL 5 CpEPDATA@DATA@DATA@DATA@DATAy DATAy DATAy rDATAy )dDATA >}Z^DATA >}`DATA >}TbDATA >}dDCHTDSamDySN 4Dye#DyeN4DyeN4DyeN5DyeN5DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 4EVNT 4EVNT4EVNT4FTabyFVocyFWO_GATCFeat//yGTyp!!4HCFGCEHCFGHCFGHCFG==InScInVtLANE?LAST~~jLIMS!!5"LNTD2LsrPaMCHNMODF5CMODL3730NAVGNLNE`NOISNnP1AMr]P1RLrZ1P1WDr_P2AMreP2BArrhP2RLrbPBASrrPPBASrrNPCONrrXPCONrrWMPDMFZ>PDMFNPLOCrTiPLOCrQPSZE`PTYPO%PXLBRGNmNRMXV1RMdNFRMdV1RMdXFRPrNNRPrV1RUND RUND RUND RUND RUNT  -RUNT  2RUNT  /#RUNT  7Rate 4RunN""NS/N%NfSCAN@SMED SMLtSMPLZSPACAc)SPACN~SPACAc)SVER4.0SVER kSVER,,4VScal@Scan@TUBEA7Tmpr<UserphARphCHNphDYbigphQLcphTRphTR