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H059'1&*%*759.PNSZ3/2':/="39.2##(,$-$)"; <!&"+;6&:9+8C+1GK:Ip<=Xx4<*#~?p MSf_(z.&:am jlP~?|3y U;3~<P/fZV]9H;dF '_0bc1'5hwyF0a}r~tgid>8()')                             )  + %0*!*,''# &  +%#                         (<,,,III^^^{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR* RRLLLkkk%%%///888AAAJJJRRR[[Zcccjjjrrryy}&&'''''(((())))****++++,,,,----....///000001111222233334444555556666677777888899999999::::::::::;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 28976630636940f89163fd62d04294a6Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2017-01-26_13-12_16533130Seq18007137718_K1319_psbajmadera96-Well%K1319_B5_Miconia_mexicana_psbaf_psbafKB_3730_POP7_BDTv3.mobSeqPop7z7789:=?ABBA@??@CFIJJIGGHKOSVWWWUTRPNMLKKJJJIIHFC>82-)''''&$!  %+29@JVds *HesaN9!tdWLC=9740,(%$&*-//-*((*,--+((*.4;CLWg{&Hma>n^SKD=6326>GQ[epFBR4g:scYSNLLPXev4PN=jz#jO@:9:;<=?CIQ]o_ j"mq!OyhL=8:>CIP\oZA]eV1l"uR:+!          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L!gn53;'$KwpNKcIJ.tnoUl;lLUize2ATAATCTAACAATATGTTGTGGAGTGCTACATACACGTGCTTATGACACCCCAATAATTCTGAGTAAATACAAGTAACGGTTGGGAGGGTGGGAAAGCACTATATTACCATCGATGGCCTCACTAGACTCCAATAATAGCTCTGTCACTCACATAGCGGAGGCAGGGTCTATAATGGTATGCATTATTCTACGTAGAGGGATGCTCGGTGTGGGGTTGAGGGTATATGAGGTAGTATACTATCACTCACGAGAGGTAGACCCGCATGTAGCTACGTGTATAACGAAAGTGAATGGTGAGTCTACTATGCACGGGCACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-01-26 15:12:31 -05:00AEPt7YAEPt7YAPFN Z\APXV2APrN 5SAPrV1APrX}}5\ARTNASPFASPtWASPtWB1PtVB1PtVBCTSIBufTy O-CMNT 5CTIDOCTNMO CTOwOCTTL 5 CpEPDATA@DATA@DATA@DATA@DATAy DATAy DATAy rDATAy )dDATA :uZeDATA :uDATA :uEyDATA :uDCHTDSamDySN 4Dye#DyeN4DyeN4DyeN5DyeN5DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 4EVNT 4EVNT4EVNT4FWO_GATCGTyp!!4HCFGCEHCFGHCFGHCFG==InScInVtLANE/LAST~~HOLIMS!!5"LNTD2LsrPaMCHNMODF5CMODL3730NAVGNLNE`NOIS0P1AM=z=*P1RL=z:P1WD=z?P2AM=zDP2BA==GP2RL=zBPBAS==2PBAS==0PCON==9sPCON==86PDMFZEPDMF0PLOC=z5PLOC=z3BPSZE`PTYPO%PXLBRGNmNRMXV1RMdNFRMdV1RMdXFRPrNNRPrV1RUND RUND RUND RUND RUNT  -RUNT  2RUNT  /#RUNT  7Rate 4RunN""NS/N%0SCAN@SMED SMLtSMPL&&ZSPACMSPAC0SPACMSVER4.0SVER ISVER,,4VScal@Scan@TUBEA5Tmpr<UserphARphCH0phDYbigphQLcphTRphTR 8This is the confidence range *ABI_Limits..FVocAEPt7YAEPt7YAPFN Z\APXV2APrN 5SAPrV1APrX}}5\ARTNASPFASPtWASPtWB1PtVB1PtVBCTSIBufTy O-CMNT 5CTIDOCTNMO CTOwOCTTL 5 CpEPDATA@DATA@DATA@DATA@DATAy DATAy DATAy rDATAy )dDATA :uZeDATA :uDATA :uEyDATA :uDCHTDSamDySN 4Dye#DyeN4DyeN4DyeN5DyeN5DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 4EVNT 4EVNT4EVNT4FTabX3FVocX"FWO_GATCFeat//WGTyp!!4HCFGCEHCFGHCFGHCFG==InScInVtLANE/LAST~~HOLIMS!!5"LNTD2LsrPaMCHNMODF5CMODL3730NAVGNLNE`NOIS0P1AM=z=*P1RL=z:P1WD=z?P2AM=zDP2BA==GP2RL=zBPBAS==2PBAS==0PCON==9sPCON==86PDMFZEPDMF0PLOC=z5PLOC=z3BPSZE`PTYPO%PXLBRGNmNRMXV1RMdNFRMdV1RMdXFRPrNNRPrV1RUND RUND RUND RUND RUNT  -RUNT  2RUNT  /#RUNT  7Rate 4RunN""NS/N%0SCAN@SMED SMLtSMPL&&ZSPACMSPAC0SPACMSVER4.0SVER ISVER,,4VScal@Scan@TUBEA5Tmpr<UserphARphCH0phDYbigphQLcphTRphTRf\mci2a>qN8>o Ueh,9n5g~Ri6(*24;x$}FNVUCs-113xT]o7U"y6^i_y>]y&m"o`^ kS\KQpYp&!gL-GI&7.{Mco6m~2HBIUbe1Cd}FxQZEZ,}4HUXYnuPQhoNePv7b{Wj2oFtXyy3@=?]p&)C^DZ=CHTA,h{|dsYuTF}wK[*oCfo;LY&p5S_3I|o6HvFHZ( g>}WqD@~TlW+b@cT]`AqhjYm=ND7!90Q2wQJLs/b>9*q:bw\6I\(kluO\,KZI\T(k"T'o)6yHAj}=D8m%(4Ws _ZZ^sSsYiSOpP S!oSRb;6AI8rBA(&jEaC9}RiTK(>V&bJ~FTWYhY8|kb+N4+~[{Hu#e./f<@j2QuOzcI0 Vps5\iDhkkJ/n=dNL?\U&;BYv7=Rl4%dF}_V/5dYQJ{Ko^=^R:^y\LMUD$bWz%I!|spN$t.DkrD5pY(d+{o.jaxJK[Ylid?O>JWT^Fu*t":Ey+D;UTw|]jL@A[]"TM,s"6rg8Z~9}{MPe!}!"l ppj0j!e~8WY!|s&4)DRhGlB5-u97.{M3>>//x3]wVk=.h^n@EK5llmd$XF$~!SNmW.$7KO]eOY ~?p ,AKM|9v[?|$}~?V'i~s^D\we*V&1pvaUG