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         n,,,III^^g{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRBUU NNNnnn ###---777AA@IIIRRR[[[dccllksss{|}g<<<<<<<<<;;;<<<;;<;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: c6556d5dddc943a69978aa2843914406Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-02-15_20-14_17403130Seq18115137968_K1318_PSBAFjmadera96-WellK1318_B7_Trema_micrantha_psbafKB_3730_POP7_BDTv3.mobSeqPop7z223334445679;=?ABCCBBAAABDEGIKLNNNLJGEEEFGHGEB@>====;9630.-,,*'#   %*19AJUao}=[w#2;=8/!yX4rbTH@:6310.,*('&(,05:=@BCDEFHILPV\binsy,Mp ~Y1scXPJFEFJPYco|!Z8gH0G@<]i7}i[RNOS[fu'ghF+8RW{#l\SONPU]j{R8b}~gDk.pWF=98:=BFKR[i{M(mW~4\?*   !$(,1:H^R9E'mx9Q+)L+     !!#%'*-///.,*('%#! 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N O 0N] &=FG!Xc+Qc*"N I$MK&]aS^$-6*Q%6 UaC' .X "# +"*@ %X8[;\GX# ?]9wObD#a^,R _7dJ2]Va'V=6[MH J7w+CTAJji%X/Ds&f3Ca);*/y.Whb [X}'`5hS%qU\  d@^B{Y)hM1%E93W[En  _)-XqD|A_05d @ M KNPmg6z#SiRi]9#%y_EOZ;ioXw)faSV[oLeS|XF] w58I:C7Zm J(/$(t7gGJ&lc9M$KCRRY9oHyCqA&B*PNwhu\QIV^ebfOJXSBGGPLB8AA%*==P<#tnJI)-&-%# &'"   "(  !            '          6+) 8=  E& %-9 _c,ppdJGW~2pT9 ka|D.Q=t1z0-8NmtQQ*i9,\T.9;&5mlOZ^ 9Xol}~J^bY>d71>(4e)bsA''KZoG@Y?hKO%88=&dIXT}p-#/Ej#so>Id6pL{tOv3Kg,GioN]FSlw)i$7d%V(BsA!J[;U8.nW1D47`BQ}<30B^%9 >-)##&?6/k?CYfc]7%AKW/217GK(4<Z&p#F,R1OJ(0:&+288?++1$8a#*#0)D<F,;;?=& YU`V.Uo @.)1BD;D?<A?>9> =PGE_NMVbR83h$TplIJUQwVB_L[+\+:;jG'il.*3T8XrB5Hx0R=`Da?/@*Rk%&AGQ-l2,*>f9~IiCp1{t.1S^HVk=`yI5sdZP)QTV1QsDnp]|dwCbO}f[2)BU  + yulATCTCTTACACATATGTGGTAGAGGAGCTCTCTACACGGGTATATGGCACCACTATAATTCAATATGACCCCCCAAGGGGGGGGATATTGTACCAGTAGTCCTTTCCGTGCCCCCCAAAAAAAAAAGCTCAATAACAAAGGATGGAGAAGGACGTAAAAAAAAACCTTACACTACGGAAGGGCGAACTTCCCGAAGAGGGGAAAAAAACTGAGAACAAAACTAAAGGAAAAAAGGGAAAGGAGCAAAACCCGACGGGATTATTGATTCCGCCCGTATCTAAATCTCTAGTAGTCCCTAGGGCCAGGGCCGGAGTTTTATCCAATTGGCGACCGGAATCCCCCCGCAGGCAGTTTCCAAACGAATTAAGGACCTTACTGTTTAAACAACAAAAAGGGACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-02-15 21:45:58 -05:00AEPt3AEPt3APFN APXV2APrN ŒAPrV1APrX}}•ARTNASPFASPtQASPtQB1PtPB1PtPBCTS5BufT'NYCMNT UCTID0CTNM6CTOwICTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA &8LpDATA &8Lp1VDATA &8Lp}DATA &8Lp6DCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANE=LAST~~4kLIMS!!bLNTD2LsrPaMCHNMODF ƒMODL3730NAVGNLNE`NOISP1AM&mP1RL#QP1WD)P2AM/P2BA2P2RL,PBASoPBASPCON!PCON 5PDMFPDMFPLOCPLOCPSZE`PTYPQPXLBRGNm(RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT ;RUNT -RUNT %,RUNT ,Rate RunN""S/N%SCAN3SMED SMLtSMPLSPACA`5^SPACSPACA`5^SVER4.0SVER 5SVER,,Scal@Scan3TUBEB7Tmpr<UserphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN ŒAPrV1APrX}}•ARTNASPFASPtQASPtQB1PtPB1PtPBCTS5BufT'NYCMNT UCTID0CTNM6CTOwICTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA &8LpDATA &8Lp1VDATA &8Lp}DATA &8Lp6DCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabDOFVocD>FWO_GATCFeat//DGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANE=LAST~~4kLIMS!!bLNTD2LsrPaMCHNMODF ƒMODL3730NAVGNLNE`NOISP1AM&mP1RL#QP1WD)P2AM/P2BA2P2RL,PBASoPBASPCON!PCON 5PDMFPDMFPLOCPLOCPSZE`PTYPQPXLBRGNm(RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT ;RUNT -RUNT %,RUNT ,Rate RunN""S/N%SCAN3SMED SMLtSMPLSPACA`5^SPACSPACA`5^SVER4.0SVER 5SVER,,Scal@Scan3TUBEB7Tmpr<UserphARphCHphDYbigphQLcphTRphTRa|cty8JMB*u $O\4I*[DeNq,P+C#Lc93"~zj;N&`,'Kym&FB2p$,%9tWa-cZ,\|b=-j%`3nXv^.PZeOY ~?p96UI//`c!sH3p{ ]G.-RRC(vJ4;N^YEu'+=3yHSBrRLohL)N6PagEU0T6;e=Y"sWi|Cal(i#5h1g9%TFIO>^7ghRS|6dc+GHO5QqEi>o.B3@dSOK[-z|G0_fs_c&a~4~kJF96YZ{sbW)WjmH_T+hSZy&m3HPz/V3QEMa!1j"SD=;LgqK-o?7.Pv_*NsPB-N&W-dOsWpC@"i@3}cUV'4MYbC*TH]pD,VS&sF{LbzMiWJ+yN"J9'!hvRdZY`X {1tMo])} ~T>G$~I!|spKa3gDkFT16Y=*"9.b':8 v/#wCdNmbcnkE=+4[q"CsINi@C,C12Mm|!Y<,h`\wmj7~.<{[t*`Q<,{AmXXt;*hZyP4)@Dx`575^@!&M Z -wqTA)y]Cj,xGJE(Rn1kZHDQ44A:_D\a$Fl@i2ps'Mvn}4|Md^_w&;H26t{_"#k<>Dj&['KD7I 95[Xv,Kho|[E,fcd]R03Wi8b9s|l}$rv/Z-\dXm4c1/`WajZUTw|]jl@i&RCSwToN6|1Dlag&<{[;N`Vn!<|'kT'_C:[rn{.QHT/RD8z{KJ[.z^`A #pJEK^;S4fT{-*<^k'eaK|`Q@#=,`Pf)NKo-Z&@GAI1_]GhnWe^kQtAcP)>`#6E1_@dUa_eM~|[{0%4dc{-m'i2}oGXS:.\_'[wA#Q9?kJ:h6Z]}4nXL-`S_YT{jqT7`h?kXM%YVJ|Q}.z zFTK(>V&j"SD=y!.qK|zW,DK >x(Y7+rIel1}ssb[l"G>z x:H-p@vdkel{F&+?cfoQe=N^tpuze/%{M*AqbEdG9}__';_/d@ikjF=mJGLbva].p>x'[IoOoh-gvxAEPt3AEPt3APFN APXV2APrN ŒAPrV1APrX}}•ARTNASPFASPtQASPtQAUDTRbB1PtPB1PtPBCTS5BufT'NYCMNT UCTID0CTNM6CTOwICTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA &8LpDATA &8Lp1VDATA &8Lp}DATA &8Lp6DCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabDOFVocD>FWO_GATCFeat//DGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANE=LAST~~4kLIMS!!bLNTD2LsrPaMCHNMODF ƒMODL3730NAVGNLNE`NOISP1AM&mP1RL#QP1WD)P2AM/P2BA2P2RL,PBASoPBASPCON!PCON 5PDMFPDMFPLOCPLOCPSZE`PTYPQPXLBRGNm(RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT ;RUNT -RUNT %,RUNT ,Rate RunN""S/N%SCAN3SMED SMLtSMPLSPACA`5^SPACSPACA`5^SVER4.0SVER 5SVER,,Scal@Scan3TUBEB7Tmpr<UserphARphCHphDYbigphQLcphTRphTR