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'!'OAO9=IULZG215#%  &2FPe]uswrzPRC*+    d,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR>VVVNOOnnn$$$...989BBBKKKTTT]]]eeemmmuuu}}}<<<;;;;<;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 9f3b519bbaa3406b9d7ce6a09959d42aSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2017-02-01_19-06_16713130Seq18031137761_K1317_MX_psba3fjmadera96-Well!K1317_H1_Asteraceae_psba3f_psba3fKB_3730_POP7_BDTv3.mobSeqPop7z b CYwvVEn'9m:"T=/ !.3.%!%0<?4 !<ML;! %67"DKaWXv6?LNW UV6YaJD?&x. =Am^[1P >(D@/veT= Oi%%Bw6$$ .5'_j\Rh 6z, 5_]9E* !"/"L" !!#$%x(,R/2G4*578; =^?AsC.EGcJ@MiPfRTsUVX[4^cadvfgijil?n^pPqr5rMrs}ujxz|b|{zVxwIvvEutsoqonmxl#jgb^SYV9SQPOHMcJGDAl>/:741/,*(B%">ZR `6Dk$E^eAkZ~vv/b:{&~akF   hr0|qiD!R+3O%'e;NgqTbm{IN[([p( 1nn:%*" 0`r> B*\XmmLK-7 _(+!#*%-OfhT3 8fa'ZS`j0QM`o% V-DPF$))jb ;{;408Nf&@\)r<  ""!CcqcB#=qKn +/L]  1 z J p $ T+''*un' yha{a&P$o:R-A0cs  mj{/Fo!okIwXyv PtnJ%9}/ E?e. 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(/-%    ,?GA/&,% 4ioA 5IQL;$ 1FQN>' 2K^f_L4   )QtzY/ !#-@E<* 0=?4 &0- /<<0  /L__M07SjutfO4   !!  %!-LbeT7   0OegT3GqtS2  096'3FLC1 '.,#5:02FJ<# ,;:, /EPNA. &14-  /^a*3hzK7YrzoS0 'F`qwqeVIA@DLSUQD3  %9LZ_VC+  0BMK<&>fc9 &$ *0, 3NenfO1   .BQVPB3)(0:BA7' %<MRH4  2@FEA@DHHA3!Bz?AMA!~ADAOvKB.bcpKB_3730_POP7_BDTv3.mobtermAGCCCCTCAAAAGAATGGGGAGAGTTTTATGGTGGGAGGGAGAAATAAACTTTGGTGTTGTTTTTAATGGGGGAAAGGGGGTTGTTGGTTTTTTTAATAGAGGATAGGCCTTGGTTGGGTGGGGGGGGGGGGGGATTAATATTAGCCCCTCAAAAGAATGGGGAGAGTTTTATGGTGGGAGGGAGAAATAAACTTTGGTGTTGTTTTTAATGGGGGAAAGGGGGTTGTTGGTTTTTTTAATAGAGGATAGGCCTTGGTTGGGTGGGGGGGGGGGGGGATTAATATT "4APWdu*5CWjs~'4DLT`iq&.6>GPXaiy'3?IT^o}!0:BQXfo}'/?HR`kv "4APWdu*5CWjs~'4DLT`iq&.6>GPXaiy'3?IT^o}!0:BQXfo}'/?HR`kv                                      #="G 43[?QCaHB\O58.@`ZSG4+P$+_XD]@E_A3K07'M3^H=TY#G1W7 _CaZ` ()*U* R7a 2I\@N:E YH`9`0MOP]a01N0>;D\QR_.>4CV(A#L- V4S/dS$"'~BUJoF />\9zbIwmt f9 e! +%(  ( advd)3BAX;'u X`Kda2gt*R0F^  rcIp (tA4P~\D (fiv*WBw2)[7zO1EMRHO_<n `/),I0)RMQ t&    .`xL@V`Sp,4C }XRw@Zxx1hTdp3x,y|8nc,lM\)\ L8jq (^X~%\pRe$l+Z[_i0q*n $C6Nbqa.O,-T#(\42;9+(UPR""Qj"E&EyPWnFI rP:d"Eu)]HxGCGTATCTCCTGCCCCCCCCCTTAAAGGGGCTCTTTGATACACGGAGGGGGCCCTGTGCCCCCCCCCAAAAAAGGGAATCTGGTGGTTCAGGGAGGGATATTTCATCTGGGGACGAATCGTTAATTTAATTTTAGGAGGGTACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-02-01 20:53:11 -05:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt GASPt GB1Pt FB1Pt FBCTSBufT JCMNT 0}CTIDJmCTNMJsCTOwJCTTL 0tCpEPDATA@DATA@DATA@DATA@DATA DATA ZDATA &DATA %DATA +WTDATA +WXDATA +WDATA +W[DCHTDSamDySN 0UDye#DyeN0`DyeN0eDyeN0jDyeN0oDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT0EVNT0FWO_GATCGTyp!!04HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~@LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISpP1AM9P1RLP1WDWP2AMP2BAP2RLuPBAS2PBASPCONPCONPDMFTPDMFPLOCPLOCPSZE`PTYPJPXLBRGNmJeRMXV1RMdNBARMdV1RMdXBQRPrNJ3RPrV1RUND RUND RUND RUND RUNT RUNT 5RUNT RUNT 3Rate 0(RunN""JCS/N%hSCAN@SMED SMLtSMPL""TfSPACMSPACSPACMSVER4.0SVER SVER,,/Scal@Scan@TUBEH1Tmpr<UserphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt GASPt GB1Pt FB1Pt FBCTSBufT JCMNT 0}CTIDJmCTNMJsCTOwJCTTL 0tCpEPDATA@DATA@DATA@DATA@DATA DATA ZDATA &DATA %DATA +WTDATA +WXDATA +WDATA +W[DCHTDSamDySN 0UDye#DyeN0`DyeN0eDyeN0jDyeN0oDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT0EVNT0FTab#FVocFWO_GATCFeat..GTyp!!04HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~@LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISpP1AM9P1RLP1WDWP2AMP2BAP2RLuPBAS2PBASPCONPCONPDMFTPDMFPLOCPLOCPSZE`PTYPJPXLBRGNmJeRMXV1RMdNBARMdV1RMdXBQRPrNJ3RPrV1RUND RUND RUND RUND RUNT RUNT 5RUNT RUNT 3Rate 0(RunN""JCS/N%hSCAN@SMED SMLtSMPL""TfSPACMSPACSPACMSVER4.0SVER SVER,,/Scal@Scan@TUBEH1Tmpr<UserphARphCHphDYbigphQLcphTRphTRMKdkij7"URQ`)e3T[.3Q~/]nLa0#C"4kyESilV'~\F>9HbM|(%: h4qKl}M2":Da^?vftu3ky+a/hV6XD9=6W\!3[s?z5O`Sdk-~4Kb}wLKkD[&C#]-@f9kf'zbO\8b790-I;dfAY(y'tMz/yxf/5f0]/joM`)6-_X.> %9WK] O7e<>/D?*&qDp-o+9mW'cj!Q4sIRD8z{q>t[XAn@:g-0!'*-%CwM#HifK1?HKz!{bmKB_,qz7A*k|_Mt0mP`k(U.*)./&0@ejUZo-vNIGQ5EYLYswo,n`!3lCIezQd)l'[)VBT0?OC5wz^`Akc PkWhIdp"iK]jLH#[,TcM#yKAI8rBAlI{_*.9nWFa-st2hSXdF#lQ%cy-*7y 4/a"XN.3ZM'3B.7 G^1mQ;R#Glue\fKZI\T(k"T'o)6yHAj}=D8m%(4Ws _ZZ^sSsYiSOpP S!oSRb;6AI8rBA(&jEaC9}RiTK(>V&bJ~FTWYhY8|kb+N4+~[{Hu#e./f<@j2QuOzcI0 Vps5\iDhkkJ/n=dNL?\ &N6Y5#2_C~T[;}IrZ:gHt3U{h.IxI>N1,7`nP@>/D?**s$[-o_}4]'Z5P%?p M;8'i2{&[y3( ]'p8NpdUuk} 9`^avedsYK%FyP\[38f6Tx*&`_MS