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    !%  "#      &1BVPb_WQE;3:Xfl5 #   0!0& ** 0      " !*/29?@?@2 ) +&)-J+/=$*&        # (    &"50          &TM,,@II^^^{{{&&&999DDDXXXaaavv      ***>>>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR(`tz{{zzzxxwwvtrqqonljigedb_]\YXVTRPNMNNFFFcccyyy   ''(0/0877???FFKMMTTTZZZaaagggnmmssryyy~}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 1f9f6d7649184c5e9df330fc5b8ec92eSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-30_20-36_07003130Seq16744134747_134747mfagan96-Well K1316_G11_Indeterminate_sp_psbA3KB_3730_POP7_BDTv3.mobSeqPop7z@ABCEHKMOPRTVXZ\^`befhhijkmpruwxz}xngfo:l"3BNUUM>( }U"g4 <b,BXm|fM2p`SI@:6310/.----./0010/.-,+**))((),29CO\k|6IW_b`YN=' waN?4+$"&+3?Pi5R/Lj?KrnO:,# !,=W~O'w.D^tJ/'3ASj\ g({Q3       !!! !!"!!!!!"""""!!!!"$%%$   $[Q vp CfoEt BnJ   "$$#!   +Ky7sa^5   &+./,'  I$\\ $F "&'&#   9hAf}ETYiT !*01,$   U'7-!W"&L. 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":a??krREakB-*9Y.M:tp P=dK>:999740,,4Hr:t6D'[Q.!!.TX\4il;dVG         B .} [}_X|?#')+-/.-)#  RyVCGx'&/6=AA?91)!                  iEDN_iB/]miaO;D`&ip5~b7Q?8!   %&g u   &tyo|2       /4C|PM9         jJIn 0&S"h$3r         6LOx<,,     .P:{&        K9:td.      eB  E      8v ^y-       (7yh    %p^&9 *P     i^8U,G]           *)meb     ?'FO>> @-f|1  fpx).  7U<o*      By<i&        "_"Dn12qf(5%Q$5?B?8.""0>GID9*  T[y0}: 'apz(K' !&(% 1m>UC w9-8=<3&      "   (%m&cEj*B#@Wagq,8BGE9% -Y{}V^<+*2>JTZ^]ZRH<2+*.8ER\a_VJ<0)).488429T+AP( K`zY`1 6TuyZB4/16::6.&!##!  e@A!wAVffAVKB.bcpKB_3730_POP7_BDTv3.mobtermATGAGTCTCTGACTAGCTGCTCTTGAGCTCCATCTACAAATGGATAAGACCGATTCTTAGTGTATATGAGTTTTTGAAAAAAAGTAGAAAAAATCCCCCCTTGGTCTAGCAAGGGGGCTTTATTTCTCCTTTTTTTTCTTTCATTATAATGAAAATGCATAGTATTTAACTGACATATGTTCTTGAAAAAACAGTCAGAAATAGATTGATTAATCTAAATAAGAGTCCTCGCTGGGGGCGGATGTAGCCAAGTGGCTCAAGGCAGTGGATTGTGAATCCACCATGCGCGAGAGGTGGATTGTGAATCCAGGGGGCGGGAGTATGAAAGTGGATCTTAGGCAGTGGATTGTGATTTCCTCATTGCGCGAGGAAGATGAGTCTCTGACTAGCTGCTCTTGAGCTCCATCTACAAATGGATAAGACCGATTCTTAGTGTATATGAGTTTTTGAAAAAAAGTAGAAAAAATCCCCCCTTGGTCTAGCAAGGGGGCTTTATTTCTCCTTTTTTTTCTTTCATTATAATGAAAATGCATAGTATTTAACTGACATATGTTCTTGAAAAAACAGTCAGAAATAGATTGATTAATCTAAATAAGAGTCCTCGCTGGGGGCGGATGTAGCCAAGTGGCTCAAGGCAGTGGATTGTGAATCCACCATGCGCGAGAGGTGGATTGTGAATCCAGGGGGCGGGAGTATGAAAGTGGATCTTAGGCAGTGGATTGTGATTTCCTCATTGCGCGAGGAAG#/;IXbgy &2?Q`kw '/;FS`jv  +5DQ]jv ".:EPZeo{ #/:DP\iv(4@LXcoz'3>IUalx +7BNZgt  $ / ; H T _ j u    ' 2 > I U a m y  ! - 9 F P \ h s    " - : E P ] i u   - 9 F S _ k w *4>IQ_ly*6ER]gr!*4@NZcmv "(7C#/;IXbgy &2?Q`kw '/;FS`jv  +5DQ]jv ".:EPZeo{ #/:DP\iv(4@LXcoz'3>IUalx +7BNZgt  $ / ; H T _ j u    ' 2 > I U a m y  ! - 9 F P \ h s    " - : E P ] i u   - 9 F S _ k w *4>IQ_ly*6ER]gr!*4@NZcmv "(7C  (""%(2125*6:)4)64->>>6>/>>//>>>/>8>1>>3>;>>>>>>>>;>>>>>>>;>>>>116>>;>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>66>>>>>;;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>666>>>>>>>>>>>>>>>;>>>;;>>>>>>>>>>>>>>>>>>>>;>>>>>>>>;8;.81"132"2.)) "."+2+3"" .$   '%             (    (""%(2125*6:)4)64->>>6>/>>//>>>/>8>1>>3>;>>>>>>>>;>>>>>>>;>>>>116>>;>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>66>>>>>;;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>666>>>>>>>>>>>>>>>;>>>;;>>>>>>>>>>>>>>>>>>>>;>>>>>>>>;8;.81"132"2.)) "."+2+3"" .$   '%             (  ELED+ M#(;9&GT`Q&1&\=Cab"ON^=.:0;2#J75)TRK3#VK1 \G0c99DaSJ. UB33S Y^9WB*UH*A#"($ O#:TP$9J[:-SKO#L6`U3 =SZ78C4aH?(>;'a7N-L>C`M:A, A- /OTD:CZ)S# A. ?'6I%BO&VNF<:P+-.cMP2M&#<+@ -Z.$W<<$"Hc\9F<50b+:> W FJ*1Y=99&B$K)1X);WUd<.Sb6HYaSV=;-P7 ADa ;&'b-K14?,TQ+#'. 5B+,YD56!MJ X*G1CSH[ v^sb8VRU<kNX;/!%i,@j =..k.GVO!FjQ{z$|U2v -?S]7bJCL=Z?b J7^1`UcViQ0))XM,tsCxBQ +-]'`SOPr9[Q2fB$/m2B? <P4rp<r ]G" s N`L~=>_UMLe4_tw]y_1|h?Y;_Fhh{B &)+X@}msx`YiT\M@oeS>E>C-&2-(8I)6'.%(')3    , x"YN# xtv* <3 (cG MpppmUQ%yX$Hdop%fOX&]@XcO,d,@ib?NEE=6q|p_d k+db?d%o,<MKc$5z`PRS)-q=/6u+S(88pd},8adhJtX1W.i.y'#Sp6d2LzeVuYs~\|qtD})[D'EJj [!+3C':0*?EL%7 !  &$(0 H++,   "$ "*   ( '  !"#!=j> !>#Px1R*2SmW/6# " &il V.L*73lH)\ ?<igq3At;:}W zfXq [H;sWTAATACTCTAACAATATGTGCTCGTGATCTACATATACGTACATATGACATCTACTCCTAGTGTATAAGAACCCAAGGGGGGGTGGAGGGGGTATTTTTTCAATCTCGCTCGATAACGCAAGAACTCGGCACAAAACTCACACCATAGTGAGGGGGCGCAGTGGAAAGGTGAGGTATGTGCTCAAGGGGGCACTCTCAGGTAGATAGAGAACTCTCGGTAGGAGAGTTCTCTCAACCCGCAGAACGTTGCCAAATGGGCTAAGGCAATGAAAATTGGATTTACGAAAAACACCTATTTAGTGAGTGGTTCTAATATCATAGGACCTTTCTAGATAAGTTTCTGATTAATATTAACTTCGTAGTTTGATATTGABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-30 22:24:28 -04:00AEPt@AEPt@APFN UAPXV2APrN 2APrV1APrX}}2 ARTNASPFASPtASPtB1PtB1PtBCTS7MBufT KCMNT 1CTIDKCTNMKCTOwKCTTL 1CpEPDATA@DATA@DATA@DATA@DATA  DATA DATA qDATA ';DATA 8pUDATA 8pDATA 8p7DATA 8pDCHTDSamDySN 1Dye#DyeN1DyeN1DyeN1DyeN1DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 11EVNT 1=EVNT1IEVNT1\FWO_GATCGTyp!!1{HCFGCEHCFGHCFGHCFG==InScInVtLANESLAST~~5LIMS!!1LNTD2LsrPaMCHNMODF1MODL3730NAVGNLNE`NOISOfSc* 5P1AMu(P1RLu%P1WDu+P2AMu1eP2BAuu4OP2RLu.{PBASuuPBASuuPCONuu$HPCONuu"PDMFUPDMFPLOCuPLOCuPSZE`PTYPKPXLBRGNmKRMXV1RMdNCRMdV1RMdXCRPrNKzRPrV1RUND RUND RUND RUND RUNT $RUNT /RUNT RUNT Rate 1oRunN""KS/N%SCAN@SMED SMLtSMPL!!USPACAmóSPACSPACAmóSVER4.0SVER 7BSVER,,1Satd*Scal@Scan@TUBEG11Tmpr<UserphARphCHphDYbigphQLcphTRphTR This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN UAPXV2APrN 2APrV1APrX}}2 ARTNASPFASPtASPtB1PtB1PtBCTS7MBufT KCMNT 1CTIDKCTNMKCTOwKCTTL 1CpEPDATA@DATA@DATA@DATA@DATA  DATA DATA qDATA ';DATA 8pUDATA 8pDATA 8p7DATA 8pDCHTDSamDySN 1Dye#DyeN1DyeN1DyeN1DyeN1DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 11EVNT 1=EVNT1IEVNT1\FTabEFVocEFWO_GATCFeat//EhGTyp!!1{HCFGCEHCFGHCFGHCFG==InScInVtLANESLAST~~5LIMS!!1LNTD2LsrPaMCHNMODF1MODL3730NAVGNLNE`NOISOfSc* 5P1AMu(P1RLu%P1WDu+P2AMu1eP2BAuu4OP2RLu.{PBASuuPBASuuPCONuu$HPCONuu"PDMFUPDMFPLOCuPLOCuPSZE`PTYPKPXLBRGNmKRMXV1RMdNCRMdV1RMdXCRPrNKzRPrV1RUND RUND RUND RUND RUNT $RUNT /RUNT RUNT Rate 1oRunN""KS/N%SCAN@SMED SMLtSMPL!!USPACAmóSPACSPACAmóSVER4.0SVER 7BSVER,,1Satd*Scal@Scan@TUBEG11Tmpr<UserphARphCHphDYbigphQLcphTRphTR^pF?jA];RI<672_4CF_E[ .*ilw17*$xeK>tW\FU"7?-3U'.1P|qW>C^;=0hSvoIxT6eT*a@*_0t}{JTMF4F>hcDW4KuYGH[{OF%6+,/|8iHAj}W2yv!a!<}B]rQHfKb9hC?{r\*xu3{CW?vR#egxy{RB0+T.h^n@2dFllj_odF4N`j`$]O|QK:jin'Dg!~^ZUS+%+M;`8hC0rOHNJw9b6mtTUk1T:29GfnGO5bLhbB/t|4unL,rg#zv{?oH[{E,8#V>Ax^c~=$~!IZHpq'_C:+0Oj!Q4KK*flK|+AO5^ |J~RA)9*=mQh`]i>\,$bnsYK%FyPq6!oSR{[}7&0AS_7WX773V[R"\"@-bWF'zPz\]G!3@*7_6; 0|&6X`??}@8jaA]!lZbCd]s:goT|gmktayr=u4[PngD]jTj.uFr~o[|Yya,mgrh~nRnX@EV$2kE}:>&ZgL7:tl|y\@fKl\!:"yXQFd7RIn6QU{A!PkB.7 T^B$*!K#Z]Pk=o?eU-:.<)%,8#V>Ax`X~lTmt.qK|-o*.AQ>UA!%EeKOWQ`QV-!w2l89+uTE#!#hmmfc4hAo?e3{0+oiw87[%Vr6R[Ule,rS]RnjH,3LiPtj glX ,cW`}F)h>?[ha_DMOFU>^[?|&Rv3672Z)~6bI.J:*yEwiOM#Y\V&&OE6OLogXY@ZB 3O{ ~VJ(/on}sF{AR8=(V-OGC#!^MrrG]iQkij7!}WQ`QEJ9."G>}O!:i1At*ldAT/n@6TdO{7+VDSHM6FNmMOx^.9,(B~b4O{[=Ku%9tWa-cZ,\|b=-j%`3EFilpAEPt@AEPt@APFN UAPXV2APrN 2APrV1APrX}}2 ARTNASPFASPtASPtAUDTU{B1PtB1PtBCTS7MBufT KCMNT 1CTIDKCTNMKCTOwKCTTL 1CpEPDATA@DATA@DATA@DATA@DATA  DATA DATA qDATA ';DATA 8pUDATA 8pDATA 8p7DATA 8pDCHTDSamDySN 1Dye#DyeN1DyeN1DyeN1DyeN1DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 11EVNT 1=EVNT1IEVNT1\FTabEFVocEFWO_GATCFeat//EhGTyp!!1{HCFGCEHCFGHCFGHCFG==InScInVtLANESLAST~~5LIMS!!1LNTD2LsrPaMCHNMODF1MODL3730NAVGNLNE`NOISOfSc* 5P1AMu(P1RLu%P1WDu+P2AMu1eP2BAuu4OP2RLu.{PBASuuPBASuuPCONuu$HPCONuu"PDMFUPDMFPLOCuPLOCuPSZE`PTYPKPXLBRGNmKRMXV1RMdNCRMdV1RMdXCRPrNKzRPrV1RUND RUND RUND RUND RUNT $RUNT /RUNT RUNT Rate 1oRunN""KS/N%SCAN@SMED SMLtSMPL!!USPACAmóSPACSPACAmóSVER4.0SVER 7BSVER,,1Satd*Scal@Scan@TUBEG11Tmpr<UserphARphCHphDYbigphQLcphTRphTR