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"        9..DAC2:   !%  #-"6''  #   $  &,,!#    "(       &0+0'   ( '& )1+=C;C`S:3(!         $) $"(&* +%+ &+ +'.#$-3-%  &0% -""(!&       #-7D)?5<825HV_GB$) $%4I9$)/"(,/+%%&2,H+:=:6++$J6B+$,, * %    %"  ! #3-))      [\]^_`abcdefghijklmnopqrstuvwxyz{|}~[\]^_`abcdefghijklmnopqrstuvwxyz{|}~,,@II^^^{{{&&&999DDDXXXaaavv      ***>>>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR(`tz{{zzzxxwwvtrqqonljigedb_]\YXVTRPNMNNFFFcccyyy   ''(0/0877???FFKMMTTTZZZaaagggnmmssryyy~}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 0cac373e762d4323b450c62a8618db05Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-30_20-36_07003130Seq16744134747_134747mfagan96-WellK1316_F3_Cobeae_sp_psbA3KB_3730_POP7_BDTv3.mobSeqPop7zoqx 9Sn 0Sv 4]1Pjwht`[3u]K;&kxc\oh7 uk`TI?720/00.+&$'4Lo,Z/p)Za&t:_/ywx{H>e$kH.Z>pA,fgfO7!ylIi+.k.FvxJ8t2Bqr0XO]uk tc[YZ\\]_ckyb<nQ, 2`@/@XiJX%`D5//27<ADDB>83.*'%#"      !###" -M*doJZMfTH?95469?EJMNLHA:2+$)Ad9@2Y8! 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"+4<ABBBDKU`hkfZJ8*  $''&##)/22.&$8Qiz~t`F.#%$ !!!7M^d_Q>+ (;KSQF5"%:NZ^XK:* !  %(&!    )..)   $**%   -E[jnfU?)     0Ibt{udL4  "$#$8Rk~ygUIGMYelldVE7,''*/6<@CCCCDHNXdsvmr *4$xedo|w`B''J~{T9.2AXsu`UXimXUhImqSpg,OS7]EBPjqV@2.1;FOROC3! )HkrS8$                KA'A+AK}VAKB.bcpKB_3730_POP7_BDTv3.mobtermTTATGTACCATTGACTAGCTGCGTGTCGAGTTCCATCTACAAATAGGATAAGACTTTGGTCTTAGTGTGTCTGAGCTCTTGAAAAATAAAGGAGCAATGCCAACCCTCTTGATAGAACAAGAAATTGGCGATTGCTCCTTTATTTTAGTATTTTTATTTTTAGTAATAGTTAGTTGACTTTAAATAAATTTTCTTTTTTTTTTTTTTTGACTTTTGTATTTCTTTCTTTTAAGACTTATAAAAAAAAAAAGGTCAAATTGAAGGGTAAAGGCAAAAAAAGAAAAACCTCTTAATTTTTGAAAATGGAATTTCAACAGCAGCTGGGTTTAGGGGGAAATTATGAGCATTTCGTTCCTCCACACCCGGGAGAGTACCGCATAAACAGAAAATATTCTTTTTTATGTTAATGATTCAATCTTGTAATACTACTTTTTATTTGTGGAGCGGATGTAGCCAATTGGATCAACGCGGGGGAGAGTGAATCCCCCCCCCCGACGAAGGATTATGTACCATTGACTAGCTGCGTGTCGAGTTCCATCTACAAATAGGATAAGACTTTGGTCTTAGTGTGTCTGAGCTCTTGAAAAATAAAGGAGCAATGCCAACCCTCTTGATAGAACAAGAAATTGGCGATTGCTCCTTTATTTTAGTATTTTTATTTTTAGTAATAGTTAGTTGACTTTAAATAAATTTTCTTTTTTTTTTTTTTTGACTTTTGTATTTCTTTCTTTTAAGACTTATAAAAAAAAAAAGGTCAAATTGAAGGGTAAAGGCAAAAAAAGAAAAACCTCTTAATTTTTGAAAATGGAATTTCAACAGCAGCTGGGTTTAGGGGGAAATTATGAGCATTTCGTTCCTCCACACCCGGGAGAGTACCGCATAAACAGAAAATATTCTTTTTTATGTTAATGATTCAATCTTGTAATACTACTTTTTATTTGTGGAGCGGATGTAGCCAATTGGATCAACGCGGGGGAGAGTGAATCCCCCCCCCCGACGAAGGA "*6>GR^j"1<IZiu $2:FQ_kv %3>JVdq{(5BNYep{ $1:ER^is~&2=JVan{+5@MZfr| %2<IVbmx   ! - 9 E P \ h t    & 2 > J W c o {   + 7 C O Z f q |  % 1 = I V a l x    * 4 B N Y d p |  -8EQ]gs %1=HTalx(3?KWbku $/AP[hy ",9AQ\hu'3>LValy,7CNZfq)6?LWeq} %-9GT_ju~  ,9DP "*6>GR^j"1<IZiu $2:FQ_kv %3>JVdq{(5BNYep{ $1:ER^is~&2=JVan{+5@MZfr| %2<IVbmx   ! - 9 E P \ h t    & 2 > J W c o {   + 7 C O Z f q |  % 1 = I V a l x    * 4 B N Y d p |  -8EQ]gs %1=HTalx(3?KWbku $/AP[hy ",9AQ\hu'3>LValy,7CNZfq)6?LWeq} %-9GT_ju~  ,9DP  !  !* )4!% +0&1$+/+&&( $ #*;,;+3+.;$;;>+28113;311322;;28231>11"13""$''++)"".+1"+8&'&('";1)1."$'"+11'')1383133833.+)"((1"++1%.2.6.&213332-33"+""2".2332.23333+"((+"+((($#+"()+"" "0.3+(3"("%")"3$             %            ""                           !  !* )4!% +0&1$+/+&&( $ #*;,;+3+.;$;;>+28113;311322;;28231>11"13""$''++)"".+1"+8&'&('";1)1."$'"+11'')1383133833.+)"((1"++1%.2.6.&213332-33"+""2".2332.23333+"((+"+((($#+"()+"" "0.3+(3"("%")"3$             %            ""                         C8 \. .XI=- <3_#CN2b3]KPX )bI@!7aL!d4DOI.K!Z$ A2 G0ANRX"N@3L2]7G3K:"W`>>%??J `@N=K:+&cYNcCAcPPKG,(N=OHB 3J*915dZM 5 DNS 'HYc<U+>I&]&:I7)2O Y9K 5OD4Y.]/?E^1$GR.- 95785*9RT1]M#R+ !J8[XU#BC0)8<H,W#0VK R. 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KB 1.4.1.82016-06-30 22:23:21 -04:00AEPt@AEPt@APFN UAPXV2APrN 2 APrV1APrX}}2ARTNASPFASPtGASPtGB1PtFB1PtFBCTSghBufT KCMNT 1CTIDKCTNMKCTOwKCTTL 1CpEPDATA@DATA@DATA@DATA@DATA  DATA DATA yDATA 'CDATA =PzUDATA =PzЂDATA =PzK"DATA =PzDCHTDSamDySN 1Dye#DyeN1DyeN1DyeN1DyeN1DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 19EVNT 1EEVNT1QEVNT1dFWO_GATCGTyp!!1HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~eLIMS!!1LNTD2LsrPaMCHNMODF1MODL3730NAVGNLNE`NOIS@jOfSc+ 9P1AMT9P1RLPMP1WDX%P2AM_P2BAcP2RL\PBASBPBAS@PCONNWPCONLaPDMFUPDMF@PLOCHuPLOCDPSZE`PTYPKPXLBRGNmKRMXV1RMdNCRMdV1RMdXCRPrNKRPrV1RUND RUND RUND RUND RUNT $RUNT /RUNT RUNT Rate 1wRunN""KS/N%@bSCAN@SMED SMLtSMPLUSPACAwSPAC@zSPACAwSVER4.0SVER g]SVER,,1 Satd+Scal@Scan@TUBEF3Tmpr<UserphARphCH@phDYbigphQLcphTRphTR This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN UAPXV2APrN 2 APrV1APrX}}2ARTNASPFASPtGASPtGB1PtFB1PtFBCTSghBufT KCMNT 1CTIDKCTNMKCTOwKCTTL 1CpEPDATA@DATA@DATA@DATA@DATA  DATA DATA yDATA 'CDATA =PzUDATA =PzЂDATA =PzK"DATA =PzDCHTDSamDySN 1Dye#DyeN1DyeN1DyeN1DyeN1DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 19EVNT 1EEVNT1QEVNT1dFTabuFVocuFWO_GATCFeat//uGTyp!!1HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~eLIMS!!1LNTD2LsrPaMCHNMODF1MODL3730NAVGNLNE`NOIS@jOfSc+ 9P1AMT9P1RLPMP1WDX%P2AM_P2BAcP2RL\PBASBPBAS@PCONNWPCONLaPDMFUPDMF@PLOCHuPLOCDPSZE`PTYPKPXLBRGNmKRMXV1RMdNCRMdV1RMdXCRPrNKRPrV1RUND RUND RUND RUND RUNT $RUNT /RUNT RUNT Rate 1wRunN""KS/N%@bSCAN@SMED SMLtSMPLUSPACAwSPAC@zSPACAwSVER4.0SVER g]SVER,,1 Satd+Scal@Scan@TUBEF3Tmpr<UserphARphCH@phDYbigphQLcphTRphTR<8s*5c'iG3mwYgE@f9kfNpwQXwJ|S#+mVAruXM5Ny1RRRlq[>u}l>&IH$rv{du."sWi|Cal(D&md3Hy$hb #T)3KO]G{LY78As{ww@>(Jt6SGp9}:>JMBrC{fwZ@FrTKZfoQek>2tp(kA;M4[{nYD3RhW~^N@.]nUHw>Sxp/YgZir$G,f *"y&bJ~FT,{12.Wh?7kx`rx).fcEK$J39OQJM~EbrB%P^gW7~+lF{ryE^STt4)T(wfT;C,_,qz7.5k|_6g0ml och_B$e_4ppOxU&g_n&J;s$dUW@_G0:!ygH3~.Nf_qw8(,?P?zI*b-^Vq&!Z=c':Z^STt4)TK%FyPD[6A|zvn[Y<,h`%mI{Mb=K{_"#kI>b0FcS==6X12g[jWcgEQm*Y6i47.z.\_'[wA#Q9U,':ZKr]#Ko5qSF1_T6RA0W7yLl-j(B~W^17a.h^n@EK5llmd$XF$}IGq8->~+gx/E[fD1E?K90c_ax]]?EZp9}:>Jdq3+fFkT/n@6TdO{7>kOptpuzpEey!!fEAx8aPI^QJH){!9Y2]2rRJm@=%FEh_@dUa0u:W^$bePX-N&Wv'i2{M~yHAj}="WlKubQ2a5rjzU4o[H;E12v-T)k\Dr^5} 1{T/(J8dM!tX^"qrB%P^gW?ivd9f TA,h{|dsQ;T`h3/[zS2Mmn[/EZdwYc_`k=`<{!,8#V>Axd$XF$X?;9y8hI0.wv|$@^]?\Hif3qE`HoG83:8\_pf|r,!}&^puJ*n?Z4?C-~ipF*/$}++y;3i-jE(_y"LAX;:,bQ2a5qO Jm}Pz%I!|sp:W+DO]h^5Q1V?ORJedjY{mwYBT3 #pJEK^;S4fT{-*<^kY{zsyN}@*ohj4TIx*&`_M(_yb;E2Yh^n@2dP1jpJ6~=D:W}&O[n/bsDkFhwD~EG7&o.9OQJMrVq}d7rLyEq28B]C\]A@r9I$sY;Fi$t_SF`|zo;LY&05OPh~wA[h@t?"\=QtAcfu?*M~~+N@c'v+gK >I57%6C}B-m@7t{_6p>z.\_'[wA#Q9U,Zy6Ka;v8e7s]jL@A[]".6,x^.9M2"ZwclQ[vL}W$620eVSQ/AAEPt@AEPt@APFN UAPXV2APrN 2 APrV1APrX}}2ARTNASPFASPtGASPtGAUDTB1PtFB1PtFBCTSghBufT KCMNT 1CTIDKCTNMKCTOwKCTTL 1CpEPDATA@DATA@DATA@DATA@DATA  DATA DATA yDATA 'CDATA =PzUDATA =PzЂDATA =PzK"DATA =PzDCHTDSamDySN 1Dye#DyeN1DyeN1DyeN1DyeN1DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 19EVNT 1EEVNT1QEVNT1dFTabuFVocuFWO_GATCFeat//uGTyp!!1HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~eLIMS!!1LNTD2LsrPaMCHNMODF1MODL3730NAVGNLNE`NOIS@jOfSc+ 9P1AMT9P1RLPMP1WDX%P2AM_P2BAcP2RL\PBASBPBAS@PCONNWPCONLaPDMFUPDMF@PLOCHuPLOCDPSZE`PTYPKPXLBRGNmKRMXV1RMdNCRMdV1RMdXCRPrNKRPrV1RUND RUND RUND RUND RUNT $RUNT /RUNT RUNT Rate 1wRunN""KS/N%@bSCAN@SMED SMLtSMPLUSPACAwSPAC@zSPACAwSVER4.0SVER g]SVER,,1 Satd+Scal@Scan@TUBEF3Tmpr<UserphARphCH@phDYbigphQLcphTRphTR