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"#       '    ",)(                                      #$%&'()*+,456789:;<=>?@ABCDEz{|}~ % & ' ( L M N h i j k l s t u v w x y z    # $ % N O P l m n o p q y z { | } ! , - . / 0 V W d p q r L M N      #$%&'()*+,456789:;<=>?@ABCDEz{|}~ % & ' ( L M N h i j k l s t u v w x y z    # $ % N O P l m n o p q y z { | } ! , - . / 0 V W d p q r L M N ,,@II^^^{{{&&&999DDDXXXaaavv      ***>>>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR(`tz{{zzzxxwwvtrqqonljigedb_]\YXVTRPNMNNFFFcccyyy   ''(0/0877???FFKMMTTTZZZaaagggnmmssryyy~}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 6d4a204dff024bfbb2a8d4193fe964acSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-30_20-36_07003130Seq16744134747_134747mfagan96-WellK1316_F1_Vitis_sp_psbA3KB_3730_POP7_BDTv3.mobSeqPop7zv :Vfu<d 0Tu  |~hJ+ sB~vuvuodVG9-% yndZQI?4(   ! 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"" 0/5% <l&|'D#  (@7'#%0=,-+"29:"**#7~,(68-_rM _b?GGAGCGCAAAACCTCGCGCCGGCGTGCGCTCTACCACCACGTGCATACGACACCTCCTGACCTAACATATGAGTGCACCCGGGACGGGGGAAGAGCCGGCGCAAAACTCCCGAAATAACCAGAGAAAGGGGACTCTTCCCACAATAAATTACTCTAAACGTCCCACAGAGAGTGGAGGGGTATGGCGAAGGCCCAATAGAGGGTTGGTCGAAGTGCGCGGGATATCCATAAAAGGGAGGGGAGGGGGAGGATAGATAACTCTATATCAGATTCACCTTTCGATCAAGTAAGGAAAAATAAATTCCAATCGAGCTCCCTCGCGGTCATCCGTGGAAGCCCAAGACGGGGGGATGGGTCCAAGTGGACGGAGGCAGGGGCGAGGGAGGCATCCCAAGACGACGTGTGGGGGCAGGGGGAGTTAATTCTTCTTTATTATTGGAGCCCCGCCGGAATATTCCCCCAGGGCGAAACAGCCGGGAAATGGTAATTACTCGGACGGGCAGAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-30 22:23:04 -04:00AEPt@AEPt@APFN ^APXV2APrN :APrV1APrX}}:ARTNASPFASPtuASPtuB1PttB1PttBCTSl8BufT TCMNT :CTIDTtCTNMTzCTOwTCTTL :{CpEPDATA@DATA@DATA@DATA@DATA DATA gDATA &1DATA /DATA <y^DATA <y1DATA <yQDATA <yaDCHTDSamDySN :\Dye#DyeN:gDyeN:lDyeN:qDyeN:vDyeWDyeW8DyeWSDyeWgEPVt!4EVNT 9EVNT 9EVNT: EVNT:FWO_GATCGTyp!!:;HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~jLIMS!!:LNTD2LsrPaMCHNMODF:MODL3730NAVGNLNE`NOISEOfScB P1AMXP1RLTP1WD\P2AMdP2BAhP2RL`PBASG-PBASE4PCONSPCONQ PDMF^yPDMFEPLOCMPLOCI&PSZE`PTYPTPXLBRGNmTlRMXV1RMdNLHRMdV1RMdXLXRPrNT:RPrV1RUND RUND RUND RUND RUNT $RUNT /RUNT RUNT Rate :/RunN""TJS/N%DSCAN@SMED SMLtSMPL^aSPACAiSPACESPACAiSVER4.0SVER l-SVER,,9SatdBScal@Scan@TUBEF1Tmpr<UserphARphCHE/phDYbigphQLcphTRphTR>偅This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN ^APXV2APrN :APrV1APrX}}:ARTNASPFASPtuASPtuB1PttB1PttBCTSl8BufT TCMNT :CTIDTtCTNMTzCTOwTCTTL :{CpEPDATA@DATA@DATA@DATA@DATA DATA gDATA &1DATA /DATA <y^DATA <y1DATA <yQDATA <yaDCHTDSamDySN :\Dye#DyeN:gDyeN:lDyeN:qDyeN:vDyeWDyeW8DyeWSDyeWgEPVt!4EVNT 9EVNT 9EVNT: EVNT:FTabzFVoczFWO_GATCFeat//zSGTyp!!:;HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~jLIMS!!:LNTD2LsrPaMCHNMODF:MODL3730NAVGNLNE`NOISEOfScB P1AMXP1RLTP1WD\P2AMdP2BAhP2RL`PBASG-PBASE4PCONSPCONQ PDMF^yPDMFEPLOCMPLOCI&PSZE`PTYPTPXLBRGNmTlRMXV1RMdNLHRMdV1RMdXLXRPrNT:RPrV1RUND RUND RUND RUND RUNT $RUNT /RUNT RUNT Rate :/RunN""TJS/N%DSCAN@SMED SMLtSMPL^aSPACAiSPACESPACAiSVER4.0SVER l-SVER,,9SatdBScal@Scan@TUBEF1Tmpr<UserphARphCHE/phDYbigphQLcphTRphTRYxr$c>'/_swW^$oVv+rrbf.f7y>i@UebYWg\I5Cd*9_krYZ=kZw4n,7]Ajci(l{~Gj`X0?888%g^^4tX {1t;#G)jp~0,W(YzXrDWy?0T4`FGN9\47.6,8efPmE$w^6Rwe7sE^Z{F9LwU$<;172+C~6bfm{l|?"wdp$^nPz/V3QEMa!1j"SD=;LgqK-o?7.Pv_*NsPB-N&W-dOsWpC@"i@3}cUV'zK\__KdrW*`)l@.u!n`%SzXbhe >k94uL'Jd"*+8@/S(`CW7;Kn|?I)_|_n?\h["#87OF4Wx4Kh#Vl5A|{IGg-' 'DvddD\4A$a{|ds:MkOVc2(_CL,v.h}{ZE!]Rc{_@w,iP;YCWeq }X'>n~~2q>-kb+N4+07> YlGcRb>@iG-@yy3@=?]p&qN<;u4#k:X=<;s_|g+;Np@YS{j4zW2`&TA]BGmb,tJLVJu6v;(vJnqG\buk'"5-z]G3o6Rl>Mm[*&`_M%0g'^+oL.SVX*os7}Z&['K2Fy^q3rjWcgDkFT16Y=*"9.b':8 v/#wCdNmbcnkE=+4[q"CsINi@C,C12Mm|!Y<,h`\wmj7z3bj6[/3-6)SE8pT/@MOsijd(DUfbLb[|!%Z@j7}:EB<~[Vt:(`iDhcL(+gbk1^DR!_TcM#yKA[,5S[l'ny#RqAEPt@AEPt@APFN ^APXV2APrN :APrV1APrX}}:ARTNASPFASPtuASPtuAUDT~~fB1PttB1PttBCTSl8BufT TCMNT :CTIDTtCTNMTzCTOwTCTTL :{CpEPDATA@DATA@DATA@DATA@DATA DATA gDATA &1DATA /DATA <y^DATA <y1DATA <yQDATA <yaDCHTDSamDySN :\Dye#DyeN:gDyeN:lDyeN:qDyeN:vDyeWDyeW8DyeWSDyeWgEPVt!4EVNT 9EVNT 9EVNT: EVNT:FTabzFVoczFWO_GATCFeat//zSGTyp!!:;HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~jLIMS!!:LNTD2LsrPaMCHNMODF:MODL3730NAVGNLNE`NOISEOfScB P1AMXP1RLTP1WD\P2AMdP2BAhP2RL`PBASG-PBASE4PCONSPCONQ PDMF^yPDMFEPLOCMPLOCI&PSZE`PTYPTPXLBRGNmTlRMXV1RMdNLHRMdV1RMdXLXRPrNT:RPrV1RUND RUND RUND RUND RUNT $RUNT /RUNT RUNT Rate :/RunN""TJS/N%DSCAN@SMED SMLtSMPL^aSPACAiSPACESPACAiSVER4.0SVER l-SVER,,9SatdBScal@Scan@TUBEF1Tmpr<UserphARphCHE/phDYbigphQLcphTRphTR