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" # $ % < R S i ,,@II^^^{{{&&&999DDDXXXaaavv      ***>>>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR(`tz{{zzzxxwwvtrqqonljigedb_]\YXVTRPNMNNFFFcccyyy   ''(0/0877???FFKMMTTTZZZaaagggnmmssryyy~}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 719b91a5eadb490caec8a065c9fc3335Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-30_20-36_07003130Seq16744134747_134747mfagan96-Well$K1316_F10_Plukenetia_volubilis_psbA3KB_3730_POP7_BDTv3.mobSeqPop7z)Pw,8@C@6'}_;`F5) (=Un 'B\u{eL2xngb^[YWUSRSTWZ^`aaa`_^^____^^^_aflv+JihL1kRA:<GXk}[Z+LP,ayZ?'"<Zw|tmga]ZXTNF;0(#%-;OiL)1s] uB#(:JW`flw&bLmzsX+o.v`QJFEEEDCB@?><;976556665446:@DE@6' &:N]efbZSNKJJJIFC?<986530..04773+&-11/,++,./27>GPSOD4##09=;72/037;>@ABCEHJMPRSSRPMJHHIJJJGB<4-,7XoP8pG}bh^SKu>"%-4<BGJKIE?92,(%###$&),/2444447:>CJVj;@uI-#*0469=CJQTRJ<,    !(+( WEEo'qSkPz*`3 '/6=EMTWUM=*  ,~ gL\1%'-148>GQZ`b_YPG=6/*% d+U6}fiE *;ObqzzsgXI<2-+***)('&'(***)(&%&(-4;AEFDA<6, A#;2 3 "*/10.*'$"!"$&()(&#! 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'#% #%)!8$.'   !(-' %!3&E 6! !4$  ! !   )_",& 7 $$,("&"4 A#+ -! *'! %   "4=$#$   !  %.*+f$t' $   #$D9H5#%)7UD6- 9B=L%2#1&v-2l!P,4;:bv5eEQj 5@ ]{_GGGACCCTAACACAATCGCGTATAAGCGCTCCTACCACCACGTAGCCTACGCCTCCCATTTGCTACTAACGGTAGCGCATGGTAGAGCCCCCTGGCGCGGGGGAAGCGCCACCTCCTTTCTCAAAAAATAAGGAGAAAGCCCCAACTCTTCAAAAAAGAAATAAGACGAAACGGCAGGGAGAGACTCAAGGGGGAGGACGAAGGCAAAATACTGAGTAGATCAGAATGCGTGAAAAATCAATGGAAGCAAAGAGAAGAGAAGAATAGATTGCAGATTCCCATCAAAGAAGGAAAAAGAAATGCGAATCGAACTCGATCGGGTAGAGGGGGGGATGTAGAGATGGGGACTGGGAAAGTGCAGACAGAAGGCAACACCGAGGGGGTGCGCGTGGGCCTCTTCGGAGGGTGGGCGGCCCCGCCAACAAGGCGGGGGTTTTTTTATCTCGGTAGTAGAGAGGATTAGAGATTCTAAGAAAGATTCGAGTAGATGGAATCCGCCGAGCGAGGGTAATAACGCGCCGAGAAGABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-30 22:24:13 -04:00AEPt@AEPt@APFN [APXV2APrN 7APrV1APrX}}8ARTNASPFASPtfASPtfB1PteB1PteBCTS'BufT QCMNT 7CTIDQCTNMQCTOwQCTTL 7CpEPDATA@DATA@DATA@DATA@DATA DATA DATA #iDATA -3DATA ?Z[DATA ?Z8DATA ?ZZDATA ?ZDCHTDSamDySN 7Dye#DyeN7DyeN7DyeN7DyeN7DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 7)EVNT 75EVNT7AEVNT7TFWO_GATCGTyp!!7sHCFGCEHCFGHCFGHCFG==InScInVtLANEFLAST~~LIMS!!7LNTD2LsrPaMCHNMODF7MODL3730NAVGNLNE`NOISYNOfSc 1P1AMnP1RLiP1WDr5P2AMzqP2BA~P2RLvSPBAS[PBASYPCONgPCONePDMF[PDMFYePLOCaPLOC]PSZE`PTYPQPXLBRGNmQRMXV1RMdNIRMdV1RMdXIRPrNQrRPrV1RUND RUND RUND RUND RUNT $RUNT /RUNT RUNT Rate 7gRunN""QS/N%YFSCAN@SMED SMLtSMPL%%[SPACAf3SPACY^SPACAf3SVER4.0SVER SVER,,6SatdScal@Scan@TUBEF10Tmpr<UserphARphCHY|phDYbigphQLcphTRphTRAThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN [APXV2APrN 7APrV1APrX}}8ARTNASPFASPtfASPtfB1PteB1PteBCTS'BufT QCMNT 7CTIDQCTNMQCTOwQCTTL 7CpEPDATA@DATA@DATA@DATA@DATA DATA DATA #iDATA -3DATA ?Z[DATA ?Z8DATA ?ZZDATA ?ZDCHTDSamDySN 7Dye#DyeN7DyeN7DyeN7DyeN7DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 7)EVNT 75EVNT7AEVNT7TFTabFVocqFWO_GATCFeat//BGTyp!!7sHCFGCEHCFGHCFGHCFG==InScInVtLANEFLAST~~LIMS!!7LNTD2LsrPaMCHNMODF7MODL3730NAVGNLNE`NOISYNOfSc 1P1AMnP1RLiP1WDr5P2AMzqP2BA~P2RLvSPBAS[PBASYPCONgPCONePDMF[PDMFYePLOCaPLOC]PSZE`PTYPQPXLBRGNmQRMXV1RMdNIRMdV1RMdXIRPrNQrRPrV1RUND RUND RUND RUND RUNT $RUNT /RUNT RUNT Rate 7gRunN""QS/N%YFSCAN@SMED SMLtSMPL%%[SPACAf3SPACY^SPACAf3SVER4.0SVER SVER,,6SatdScal@Scan@TUBEF10Tmpr<UserphARphCHY|phDYbigphQLcphTRphTR6}k1$AZE]bZ&[VGgPH/#8|.IQK%=G{}(-TPrbJ32Qy]xwY>2hpu:In8nZZ*W]U_I[Ddg/.9u2jDTWy2>y^q3Th$Y thENWNv3[My I3)yfuq.$>2tp(kA;M4[{nYD3RhW~^N@.]nUHw>VihGxNM4pW 2:MMvdQh(AX#HTrhV KVSFsA b5}8 v/,J6v`ZeW\j//u@8Q CKOjLH#[,bTmq/*7ag%gcmeLMaCG !9W&a:OcPC]LVn!<|'{XW,@|sF4GG\}d M;Y@iGtX[3>>B%P^gWyR;uIkrE-O)\rS|5qk`T1jdRC WIFM/|RB"H2gQFil=.n2`#]>}Zjp*~tM}rEH-AC3w[0 NL/JX;MM;8'i2{q6.>`A #pJE@^*\vJnPk=o?eU:g;TaywK[*oCf`y6n?2x`agv`k=;A{%i7#W yZE!e~8WY;`.pr$,.[G57'9\47.e6bZ( iu2Zv&1`2h`_`&1hE;qT%;5]U'V[,A4#O|!faiw`6wPjgvG9 HtWo0t[XAn@xg-W!-D-%cwOqHiEKzz#Kz!{bmKB_,qz7A*k|_Mt0mP`k(U.*)./&0@ejUZo-vNIGQ5EYLYswo,n`!3lCIezQd)l'[)VBT0?OC5wz^`Akc PkWhIdp"iK]jLH#[,TcM#yKAI8rBAa^{?nNL)H"i=Q<,{AmXXt;*hZyP4)@Dx`575^@!&M?ZCb|b2x, >>d}:GSg&b0RwCyTQ[pcc??J.LHSF{,ComMOx8yT,I)_|_n?_;?oW~!^F7N? >S[jWcgEQm*Y6i47.r~+:mzv&1`Zkel{F&+?cfoQe=N^tpuzemMOMh7&NxOrz_l4~h@<}9W#()rR.X}e6bZ( ]'nya0#CFlc*{hAC?-4$&"Sf^wKB]A\9I*$6elkgahH;M[=g;^{AEPt@AEPt@APFN [APXV2APrN 7APrV1APrX}}8ARTNASPFASPtfASPtfAUDTUB1PteB1PteBCTS'BufT QCMNT 7CTIDQCTNMQCTOwQCTTL 7CpEPDATA@DATA@DATA@DATA@DATA DATA DATA #iDATA -3DATA ?Z[DATA ?Z8DATA ?ZZDATA ?ZDCHTDSamDySN 7Dye#DyeN7DyeN7DyeN7DyeN7DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 7)EVNT 75EVNT7AEVNT7TFTabFVocqFWO_GATCFeat//BGTyp!!7sHCFGCEHCFGHCFGHCFG==InScInVtLANEFLAST~~LIMS!!7LNTD2LsrPaMCHNMODF7MODL3730NAVGNLNE`NOISYNOfSc 1P1AMnP1RLiP1WDr5P2AMzqP2BA~P2RLvSPBAS[PBASYPCONgPCONePDMF[PDMFYePLOCaPLOC]PSZE`PTYPQPXLBRGNmQRMXV1RMdNIRMdV1RMdXIRPrNQrRPrV1RUND RUND RUND RUND RUNT $RUNT /RUNT RUNT Rate 7gRunN""QS/N%YFSCAN@SMED SMLtSMPL%%[SPACAf3SPACY^SPACAf3SVER4.0SVER SVER,,6SatdScal@Scan@TUBEF10Tmpr<UserphARphCHY|phDYbigphQLcphTRphTR