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***>>>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR(`tz{{zzzxxwwvtrqqonljigedb_]\YXVTRPNMNNFFFcccyyy   ''(0/0877???FFKMMTTTZZZaaagggnmmssryyy~}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 369ce7b3bcfd42dbb4080eebf975815cSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-30_20-36_07003130Seq16744134747_134747mfagan96-WellK1316_D1_Matelea_sp_psbA3KB_3730_POP7_BDTv3.mobSeqPop7z(()+-/00///01356665667765557:=?@>:659BPcxr]H6(  #&&$   "1CUh} 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MVdysia[TK@2$|[>'#9Wz$6HYlsdVI=3+%$'/<L_qjH#s[D,lQ7! )A^/_&BXgqvx{'Jjc&W`LkTFH_aK g7 lIhiKYhgVPMG;(2vjEt I}ruvb\YQB/ GJ#n8,?OWVJ4Jerbct #!^@0J`ntrhWC/ %()('(*,-,)%#%)/1, iO0{$Joy[DDd\pC~+hacfiif^PA:H{f F'>&=F; `rt!k  Y=rdgr)   MF^>@    T O`84   9A395=^A (zNjU;-D%2~# 2|&M=N   FEE^[%'y -l<9_ k+!  f4xJ~) S$s^7" *bM `pCv$b[EO-!$%&())&!  Pd- U6YP_7?a. $/.+,6FW`^M1a,nv@z(  Mc*   -|`jRQ$#++'###$#!  ;a~yY2 2pmsG|~;yWj $%"    #/8<5# yx?IQ-"6OH171% "OM   1rh"Jw:(7*?>P&#^2g%F8$P eEO   #Pu>   .Jbqy4MA< l "uupeG}G%&!   >hb8 '1<Oo?O:n0    N '!x>  (^(C8 |=  ':EC6#  *fpU    7  OJnE<6CRc       4V$r{oX{[;     &~Mg  )V<-y B( ^1    8_h@ &FilK,   #u[k/-D         Y5\ :m<--%K 'Hsq>%NX0   H{X.$\HyeH0 }O+  "\R~}P_&     zxjj2G*$ft();Rm(JafW3}Z"n4'-258;==:5.'" #(.3785/' L wfP#  O(\a)OZEBFHD<3,(%$#"$(/7@GNSW[\\XRI?5,$  ,B@ AAEMA]dKB.bcpKB_3730_POP7_BDTv3.mobtermCCAGTTCTGACTGCTTCCTGTTTGAAGTCCCTCTATCAATGGAAAGACTTTCTGTCTTAGTGTATACGAGTCCTTGAAAGTAAAGGAGCAATAACCAATTTCTTGTTTTCTCAAGAGGATTGGTATTGCTCTTTTATTATTAAAAAATTTTACATAACCTTTTTTCTAATTTCTACTTATATATAGTAGAGGGGGCGGATGTAGCCCAGTGGATCAAGGCAGTGGATTGTGAATCCACCATGCGCGAGAAAAGTTTTCAGAACGTTTTTGGTTTTTGTTCGGAAACGTTTAGGGCAAACTTATGTATATAAGAGATGTATTAAGAATCCCCCGTACGGAAGGTTTTGCCTATTAAATGTTTTGATAGAATATGGGCCCATTTTTCTTGATGAATCAAAGCCGGGTTCGACAAACCCCACACGGCGAGGAGGAGCAGGTAGTTCCTCAAGGCAAGTGTGGGGGGGGGGAGAAACAACCCGCCCCGAGCCAGTTCTGACTGCTTCCTGTTTGAAGTCCCTCTATCAATGGAAAGACTTTCTGTCTTAGTGTATACGAGTCCTTGAAAGTAAAGGAGCAATAACCAATTTCTTGTTTTCTCAAGAGGATTGGTATTGCTCTTTTATTATTAAAAAATTTTACATAACCTTTTTTCTAATTTCTACTTATATATAGTAGAGGGGGCGGATGTAGCCCAGTGGATCAAGGCAGTGGATTGTGAATCCACCATGCGCGAGAAAAGTTTTCAGAACGTTTTTGGTTTTTGTTCGGAAACGTTTAGGGCAAACTTATGTATATAAGAGATGTATTAAGAATCCCCCGTACGGAAGGTTTTGCCTATTAAATGTTTTGATAGAATATGGGCCCATTTTTCTTGATGAATCAAAGCCGGGTTCGACAAACCCCACACGGCGAGGAGGAGCAGGTAGTTCCTCAAGGCAAGTGTGGGGGGGGGGAGAAACAACCCGCCCCGAG %3?Rbn| (3:FQ[kt~#6BOYeny$0<JTalw+6ALWamy ",8DOXbo{ ".;GS_jt !,7CN[gs)4?LXcoz   & 2 ? L Y d p |   % 0 < H U a m z    * 7 A P \ i u    / > J X a k v  " - ; I U b p z  &/;JUamz#/9DV`jx *4>LWht,:I[eu !/9DO\ft~ !0AIZmt !39ELXep})5CQZcq $/8CQ\eq &/9GXes %3?Rbn| (3:FQ[kt~#6BOYeny$0<JTalw+6ALWamy ",8DOXbo{ ".;GS_jt !,7CN[gs)4?LXcoz   & 2 ? L Y d p |   % 0 < H U a m z    * 7 A P \ i u    / > J X a k v  " - ; I U b p z  &/;JUamz#/9DV`jx *4>LWht,:I[eu !/9DO\ft~ !0AIZmt !39ELXep})5CQZcq $/8CQ\eq &/9GXes                 1118>086#"  "! "!"-"$""""""""#""'"'"%..++"("+""" "(++""' +  '"' 8;>>>;;>>>>6>" $ 1 #                              "                                                  1118>086#"  "! "!"-"$""""""""#""'"'"%..++"("+""" "(++""' +  '"' 8;>>>;;>>>>6>" $ 1 #                              "                                 W =TK.1 Q SB,17aa -0N89)5( $X "43#*:T@"Pd(V' S/4;^=[DBM/ # TS%0-I5+; ?;1B ;-<W*; 1A3)MN.]0%8 .b("FH?;YYPX 6$,O ^'b[BHFbHc#HW7DA %09 /*X+PX@]T'7(DW9-4W='\12[+Q,ZG^T#JX#O]T>aH,"RX'?E;a5&?!^$a6BXS ^ 2%GbOB@B 17. _E"7\L; cQ< X01_[[!XD-RI$T@&R( SZF@RWT%@P'JTPSG EFMaZ$-R4\@HD'7 &-D2,'$.ARMV C_&?,T] >*LVF J1M&b=L$]G+) F=:'GX&2Z@N0YK18+62Bca]2*GC2 \;4cbTPb> L*?ZK4 O\SD*^FG-"E4x  2 Q\yP]KK^sb;nA,0,`gnRPrZ/%'s0>q5M"il6( R`qYG?]U @Gl)<K0vkE,b">@J_a ";5m(In_QEpxyC? UuF'%v'|#M F %qes*VMV@;Q+Pj $8Vdrx n}|Bk   Vv:v e1cqnOBD:9"vHT\!}X(KRX y)73A1<S>8wDk;o\F#; c`u$}?EZ$}MR  Pz\gS~ FA ;A  H y2% `A@x@@x@Ux@@xxdtnxz}xKxxxx(xxxx= 4@fx $% .%c a8X-+2%^C+3M# 6J6/88 CAg(>"& f  +3$  $2n<vrXT(qQs>pvLptp\X5OyK5 ;d dW\$nT,9d@|(8}Nk,wJ T&)naJ^c4wgR'2a M!}H >8%S\Hi`6d/}EQuGH|"Lo9I-f{()3N^ I2,*J d>%P8 "lc&@2?8;*zZ_'m\#:]Zp^]!p^4=T'yiG5%\'ln.%3G7u*U%;o/4.F4kE~$M:<6zDcBVOV>M*N9V"K=809-9.2dLwwmH#WLh4(}TB9,9ePM,6w`>o/\-v/B_\y&bt >q^ 7&P[) ~0{vE  #0.27iWR0C/+)0]1Es`O$9u [KY<uE]}{$rn<SCxs/sF xeRMY C&& YI9Xe8Vlz)2#>ho7i6A0,nw! y2 dX 4jdd5I#A e?)K/'z 0  0bsAATCCCTAACTAATCGGTGCGAGCGGTATAAATCCCACGGCATTGTGACCCTCCGTCCCACCGTACGTTAGTTCCCGGGTGCGGAAGAGCGGCGGTGTGCCTGGTACCGTCACCTTTTTAGACATAACGCTCCCCCGATAATCTTCTAAAATTTAGGTAAAGGGGACGGAAGTAGTCACGTGGATCAGAGCATTAGATTGTGTATTACCATTCCGCGAAACTCTATAAAGTTGGTTCAACCAACGAGACCCCAAAACTGAGTGCGCCGGTTCGGGATAGGAAGTGGCGAGCATCGTTCACGCAAGGCTCGGATAGCAGCCATTTTTAGTTAATCGATCGCTAAAAATGAAGGGGGTCCGCAGCGATTTTGGGTTATTTTCACACACCTGGAGTGTGGGTTATTAGAAATTTTTTTTTTTTTTTTTTTTTTAGTTTTTGCTAGTAGTCCCCAGTAGTGTATTCAAAAAGATGCTCCAGACGGGGAGTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-30 22:23:08 -04:00AEPt=zAEPt=zAPFN cAPXV2APrN ?2APrV1APrX}}?;ARTNASPFASPtASPtB1PtB1PtBCTS|)BufT YCMNT >CTIDXCTNMXCTOwXCTTL >CpEPDATA@DATA@DATA@DATA@DATA  DATA  DATA *DATA 4kDATA >i|c DATA >i|DATA >i|\DATA >i|فDCHTDSamDySN >Dye#DyeN>DyeN>DyeN>DyeN>DyeWDyeW8DyeWSDyeWgEPVt!4EVNT >aEVNT >mEVNT>yEVNT>FWO_GATCGTyp!!>HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~zLIMS!!?LNTD2LsrPaMCHNMODF?"MODL3730NAVGNLNE`NOISV[OfSc@P1AMiP1RLeP1WDmVP2AMtP2BAxP2RLq"PBASXtPBASVPCONcPCONaPDMFbPDMFVrPLOC^&PLOCZZPSZE`PTYPXPXLBRGNmXRMXV1RMdNPRMdV1RMdXPRPrNXRPrV1RUND RUND RUND RUND RUNT $RUNT /RUNT RUNT Rate >RunN""XS/N%VSSCAN@SMED SMLtSMPLbSPACMSPACVkSPACMSVER4.0SVER |SVER,,>5Satd@Scal@Scan@TUBED1Tmpr<UserphARphCHVphDYbigphQLcphTRphTRrThis is the confidence range *ABI_Limits..FVocAEPt=zAEPt=zAPFN cAPXV2APrN ?2APrV1APrX}}?;ARTNASPFASPtASPtB1PtB1PtBCTS|)BufT YCMNT >CTIDXCTNMXCTOwXCTTL >CpEPDATA@DATA@DATA@DATA@DATA  DATA  DATA *DATA 4kDATA >i|c DATA >i|DATA >i|\DATA >i|فDCHTDSamDySN >Dye#DyeN>DyeN>DyeN>DyeN>DyeWDyeW8DyeWSDyeWgEPVt!4EVNT >aEVNT >mEVNT>yEVNT>FTabFVocrFWO_GATCFeat..DGTyp!!>HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~zLIMS!!?LNTD2LsrPaMCHNMODF?"MODL3730NAVGNLNE`NOISV[OfSc@P1AMiP1RLeP1WDmVP2AMtP2BAxP2RLq"PBASXtPBASVPCONcPCONaPDMFbPDMFVrPLOC^&PLOCZZPSZE`PTYPXPXLBRGNmXRMXV1RMdNPRMdV1RMdXPRPrNXRPrV1RUND RUND RUND RUND RUNT $RUNT /RUNT RUNT Rate >RunN""XS/N%VSSCAN@SMED SMLtSMPLbSPACMSPACVkSPACMSVER4.0SVER |SVER,,>5Satd@Scal@Scan@TUBED1Tmpr<UserphARphCHVphDYbigphQLcphTRphTR}0+wU]C0.Bv|[Eh2!Ul628z>X}m ;gEF~ba+;x~Cm*qHE?OZC@weCS'J7)Ku3Be%{M*A#t5#r~^;?nU[PbI^6E]bZ&[VGgPH/#+-'M35FQ6n:.]X?K90plql{]j6K8eX)GE#!#+X(fcT%GY?tY%%\2D>HM6Fnf%)q.i.]X"vQ*m2WA /kXSE@a!)D$SXtmI^MHgX63g4`mA^.?c`fUw3^NU>xW#nZE35ux2]DuYkqU*{Zq9Fy$C.bT8Poo=[/19~{yrA]#_WfOMt_`VWYhY8|kRYN4+HmLP}6YH4HUXYnuPQh^fP%4X+*flT)FTX>EbrB%P^gWT|0\d&nPl'Z9dp"iK]SSK.N6jn6O1HnYDx8_b_l4P3LFRt( g>::#Q[A0Z'mJ pT[_u\v5Yw SB&$q SMOiiX>VL-&]S9Hgn_RDO6',>71B!Hiwe2{sGitGy;`4E~G{WhcS]p?_sJOh^&6X`??}@8jbX-@^"qrB%P^gWTb*\mvH%lFDI| sYq-f^DR!_TcS{[}7&0E(_y"LAX;:.z9W-y]l0Uo~e8HJj`$dUW7.m/J[Y^%X\HifcJkhi=!TMBR?Vp#Nq6;1&X76O~$1b~%AM&Z'K7rtlqaxkabC;Z6Ob"1{7"{2A2{UZBwE$X~}:>J5*m#mfXr9I&YD8J-o XS_9zH'zeE^O{U,Z:X=<;3On&+VDyK{t61h67A"yV+XZCTIDXCTNMXCTOwXCTTL >CpEPDATA@DATA@DATA@DATA@DATA  DATA  DATA *DATA 4kDATA >i|c DATA >i|DATA >i|\DATA >i|فDCHTDSamDySN >Dye#DyeN>DyeN>DyeN>DyeN>DyeWDyeW8DyeWSDyeWgEPVt!4EVNT >aEVNT >mEVNT>yEVNT>FTabFVocrFWO_GATCFeat..DGTyp!!>HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~zLIMS!!?LNTD2LsrPaMCHNMODF?"MODL3730NAVGNLNE`NOISV[OfSc@P1AMiP1RLeP1WDmVP2AMtP2BAxP2RLq"PBASXtPBASVPCONcPCONaPDMFbPDMFVrPLOC^&PLOCZZPSZE`PTYPXPXLBRGNmXRMXV1RMdNPRMdV1RMdXPRPrNXRPrV1RUND RUND RUND RUND RUNT $RUNT /RUNT RUNT Rate >RunN""XS/N%VSSCAN@SMED SMLtSMPLbSPACMSPACVkSPACMSVER4.0SVER |SVER,,>5Satd@Scal@Scan@TUBED1Tmpr<UserphARphCHVphDYbigphQLcphTRphTR