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" # $ % & ' ( ) * + , ; < = > ? @ A B C E F G H I J K L M N O P Q R S T U V W X Y Z [ \ ] ^ _ n o p q r s t u 5 6 7 8 k l m n o p q r x y z { | } ~  ( ) * + , - Y Z [ \ ] ^    t u v w x   : ; < = > m n o p q r s | } ~  P,,@II^^^{{{&&&999DDDXXXaaavv      ***>>>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR(`tz{{zzzxxwwvtrqqonljigedb_]\YXVTRPNMNNFFFcccyyy   ''(0/0877???FFKMMTTTZZZaaagggnmmssryyy~}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 1a2ed54e2239497893a826ed06a0cf93Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-30_20-36_07003130Seq16744134747_134747mfagan96-Well'K1316_C9_Lycianthes_stephanocalyx_psbA3KB_3730_POP7_BDTv3.mobSeqPop7z$/8@HPY`fkmnlid^VL@4& tligfec`\XSME;/" .D[s7Qk|jV?& reXLA80)%""$(0;GTbnz -Jm7ITXSE.^-qO8.0;M_mwzywvw}ZkH {j &I=i76e4J87@VB1 <U)3Ru.[  }@[B;CQ_iljhginqrplilv|mb\XVUTSQONKIEB@ACGHE<.-Qw||v[>" !:Un{iXMHHNVbn|ws}R$QTBA#~`Jhj%(%{~nWD=CTmrR/@f{}ubF' (OwnK*:asM' ):GNNIA:67<DKNK@/ #3?ED?83249?CC=1  0<BA;4004:>=6* /@LMD3"&I3X )?n  <G3LZc# )/1225:@C@4/zLtf)a`6" '/6<BGKOPOKFA=;;>ADFFD@90'")Eg$wTMM $%"%9lAe weF! 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UCa.=69=)_  N?^"QJE?`IF @;J-8"//J\&a!c0C( USM]52:_cWG "'[U G7?cQR )* [2$C)?V V *=V2F?NKMK4daVZ^3+L )T9G59; ?:c2 TL)T8< ; B 9O<9 *>?1U1+<` Q2X5OU3(-! [(ZU8KK T,N[J007&DN_;]#7J *WL/MUY6c/+D :>(:_% a+`aLbCcA4$ Z2_:UWRE 6`;3;5A 9'& W&R0,7#AIE^_E;<("F OVI#1I 83&K FM<F+:[Ebd;( 2?%G <29CZF4_G;4?0X X/#6AY[,?JZ, CD#&Tc:L_P@]:cQ%DWE=B0.L,7L7L\RRDAc2Y?7E0 % OAM W/>ahiua/s7C^`0pJd t8w}G5lKIkd`5(Kh%(}zT]q563KU/1qo`tJ` KS10WNY5#J!o&ML4 4C"b&4E,jL|w{y$:S[09<fLtU[b'I0v We-TA[[g+k6pW45 ,! 6"G8WCM&g?1qGgD$)Z hq@5@&IbDpp~rz!L3, 3{\'mPB:K=Cba(ub[+ M41~fq&  "(mdF`},[+ 0  ( ( ( ` @@@@@g`x{xG_p;2)3>*.4,!   D, 96)&   @MS]     O@ie3M85G0 $ND.'/J  %@&B(+,Zm] gBBwJgZn]+y+kz}M8-,IpNp0~Xa$f/\5^0F.c>#jdIE3!6Lpd[pU?r #X!?g2+ K?mC8 M=~8a F$)A@ 0sUik3^[HE)4"GOtHE,,]zk}~OMiI*8p!],\7UdeB7`_n d,G&gTF^pH|A?\Q~,8p=8p\trd7^9X??)z,_+xY|j> vV0%v}'lhf -!r;d54/Ua;~%!-/a+:?;<4(R->9R,9\@,qLML: _&JPX@6&Np3;!20&!0%'/#u;UA0CE/!*.=Q<W: D.(!i/ %$e"[~pgLpxH( i6)?!sN_e"z;H /]B X,[eqc_nyk~^B|/rT #d([f R$<j $GYeq7;0B,=~K84u0H:d5k Z#tO@"/!$nR{%8!$Z`23;#wIaLX6+Y;j"K!U&,LJ3'l:lbTgI(fR|e$ 6wvSrhG;8~V[I > v ] o^GPPAAACCAACTAGACTAATCGGTGGCCCGACTAATCCAGTACATCTCTCTTCCCACACCAATGAAGGGATTAAGCCCGCGACCGCCCTAATTGCTCTTGTAATCGGACGAGGAACCGAACGCCGGAACCCCGAATCAACCCTCCTGGACGAACGGTTGGTTTTGGGAAGATGGTATTAAAATCTGCCATACCCCGTGAAAATAATAGTAGTGCCATAATATAGAAGACGTCCGCAGATGCCTGCTTGTATAAGGACGCTGTATAGTGAGTCTACTGCCCGCGAGCAGGCCGAAAGCCTCCTTAATTAAGGCTAAGGGAAGAAAGGCCGCTGGCACAGAGCCAAGTATTACGGCGTTCACCTGCACCCTCATTAGATTTGAAGCCGAATCGTACTGCGAGGCTCATTGGTTTAAGTGTTTTTTTTTTTTTTTTTGGTCGCCAACATAGAAAGTTCCCTTGTGACCAAAAAGTACACACCCTTGTTTTCGAAABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-30 22:24:22 -04:00AEPt@AEPt@APFN bAPXV2APrN ?APrV1APrX}}? ARTNASPFASPt[ASPt[B1PtZB1PtZBCTSOBufT XCMNT >CTIDXCTNMXCTOwXCTTL >CpEPDATA@DATA@DATA@DATA@DATA DATA  DATA *qDATA 4;DATA 8qbDATA 8qԩDATA 8qFiDATA 8q)DCHTDSamDySN >Dye#DyeN>DyeN>DyeN>DyeN>DyeWDyeW8DyeWSDyeWgEPVt!4EVNT >1EVNT >=EVNT>IEVNT>\FWO_GATCGTyp!!>{HCFGCEHCFGHCFGHCFG==InScInVtLANEKLAST~~NoLIMS!!>LNTD2LsrPaMCHNMODF>MODL3730NAVGNLNE`NOIS)OfSc(P1AM=>P1RL9lP1WDAP2AMHP2BALP2RLDPBAS, PBAS*$PCON7PCON5PDMFbPDMF*PLOC1PLOC-PSZE`PTYPXPXLBRGNmXRMXV1RMdNPRMdV1RMdXPRPrNXzRPrV1RUND RUND RUND RUND RUNT $RUNT /RUNT RUNT Rate >oRunN""XS/N%)SCAN@SMED SMLtSMPL((bSPACAf\SPAC*SPACAf\SVER4.0SVER OSVER,,>Satd(Scal@Scan@TUBEC9Tmpr<UserphARphCH*phDYbigphQLcphTRphTRdThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN bAPXV2APrN ?APrV1APrX}}? ARTNASPFASPt[ASPt[B1PtZB1PtZBCTSOBufT XCMNT >CTIDXCTNMXCTOwXCTTL >CpEPDATA@DATA@DATA@DATA@DATA DATA  DATA *qDATA 4;DATA 8qbDATA 8qԩDATA 8qFiDATA 8q)DCHTDSamDySN >Dye#DyeN>DyeN>DyeN>DyeN>DyeWDyeW8DyeWSDyeWgEPVt!4EVNT >1EVNT >=EVNT>IEVNT>\FTab^SFVoc^BFWO_GATCFeat//^GTyp!!>{HCFGCEHCFGHCFGHCFG==InScInVtLANEKLAST~~NoLIMS!!>LNTD2LsrPaMCHNMODF>MODL3730NAVGNLNE`NOIS)OfSc(P1AM=>P1RL9lP1WDAP2AMHP2BALP2RLDPBAS, PBAS*$PCON7PCON5PDMFbPDMF*PLOC1PLOC-PSZE`PTYPXPXLBRGNmXRMXV1RMdNPRMdV1RMdXPRPrNXzRPrV1RUND RUND RUND RUND RUNT $RUNT /RUNT RUNT Rate >oRunN""XS/N%)SCAN@SMED SMLtSMPL((bSPACAf\SPAC*SPACAf\SVER4.0SVER OSVER,,>Satd(Scal@Scan@TUBEC9Tmpr<UserphARphCH*phDYbigphQLcphTRphTRa|cty8JMB*u $O\4I*[DeNq,P+C#Lc93"~zj;N&`,'Kym&FB2p$,%9tWa-cZ,\|b=-j%`3nXv^.PZeOY ~?p96UI//`c!sH3p{ ]G.-RRC(vJ4;N^YEu'+=3yHSBrRLohL)N6PagEU0T6;e=Y"sWi|Cal(i#5h1g9%TFIO>^7ghRS|6dc+GHO5QqEi>o.B3@dSOK[-z|G0_fs_c&a~4~kJF96YZ{sbW)WjmH_T+hSZy&m3HPz/V3QEMa!1j"SD=;LgqK-o?7.Pv_*NsPB-N&W-dOsWpC@"i@3}cUV'4MYbC*TH]pD,VS&sF{LbzMiWJ+yN"J9'!hvRdZY`X {1tMo])} ~T>G$~I!|spKa3gDkFT16Y=*"9.b':8 v/#wCdNmbcnkE=+4[q"CsINi@C,C12Mm|!Y<,h`\wmj7~.<{[t*`Q<,{AmXXt;*hZyP4)@Dx`575^@!&M?ZURwSwesy^@{ OuZE6Q1m*e)yn!QB;YDSc~Xbq&S)BcS[7}?ef5U_9}j~:HzZbti2]2rRU)>`V|I+!K|-o_}4]'b5 ]{7HFwMzR9vAMV^@c O*Pv#$6wC(vJKq&1hE;qT%;5]U' S!oSRIoM/V/5`agvb_@U])%,1+7XM&b$SXtmx+9Hgn<_swW^$beP`C:F__diM(]y"/:Boz?&qNR0Xwt2)lOY^k_l_e5ll@i2zeqr[&X0VDE?g`p}CW1>r&SPT4VfJlHQo3Yp[UR6? sI(J8dR9F![pE)}%z5dJ6vvdeW\j//u4aO/CsINi@C)Re/K)Z6AI8rBAr] {[q?Eb,f=E]rRS$~=}F)h>S]p?_sDkrD5pYlGcRbi`)lZFGz/X<7:?w5RiV1]cs:*?FvHQn:4L\\W'KFxz2jA&owbBV*d>AEPt@AEPt@APFN bAPXV2APrN ?APrV1APrX}}? ARTNASPFASPt[ASPt[AUDTn&B1PtZB1PtZBCTSOBufT XCMNT >CTIDXCTNMXCTOwXCTTL >CpEPDATA@DATA@DATA@DATA@DATA DATA  DATA *qDATA 4;DATA 8qbDATA 8qԩDATA 8qFiDATA 8q)DCHTDSamDySN >Dye#DyeN>DyeN>DyeN>DyeN>DyeWDyeW8DyeWSDyeWgEPVt!4EVNT >1EVNT >=EVNT>IEVNT>\FTab^SFVoc^BFWO_GATCFeat//^GTyp!!>{HCFGCEHCFGHCFGHCFG==InScInVtLANEKLAST~~NoLIMS!!>LNTD2LsrPaMCHNMODF>MODL3730NAVGNLNE`NOIS)OfSc(P1AM=>P1RL9lP1WDAP2AMHP2BALP2RLDPBAS, PBAS*$PCON7PCON5PDMFbPDMF*PLOC1PLOC-PSZE`PTYPXPXLBRGNmXRMXV1RMdNPRMdV1RMdXPRPrNXzRPrV1RUND RUND RUND RUND RUNT $RUNT /RUNT RUNT Rate >oRunN""XS/N%)SCAN@SMED SMLtSMPL((bSPACAf\SPAC*SPACAf\SVER4.0SVER OSVER,,>Satd(Scal@Scan@TUBEC9Tmpr<UserphARphCH*phDYbigphQLcphTRphTR