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"     Z[\]^_`abcdefghijklmnopqrstuvwxyz{|}~     IJKLMNOPQRSTUmnopqrst 4 5 > ? @ A B C D K L M N O P t u v w x y z 6 7 8 9 C DZ[\]^_`abcdefghijklmnopqrstuvwxyz{|}~     IJKLMNOPQRSTUmnopqrst 4 5 > ? @ A B C D K L M N O P t u v w x y z 6 7 8 9 C D,,@II^^^{{{&&&999DDDXXXaaavv      ***>>>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR(`tz{{zzzxxwwvtrqqonljigedb_]\YXVTRPNMNNFFFcccyyy   ''(0/0877???FFKMMTTTZZZaaagggnmmssryyy~}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: e7be33f49be54ebb9dac0a88ce1843c4Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-30_20-36_07003130Seq16744134747_134747mfagan96-WellK1316_C5_Indeterminate_sp_psbA3KB_3730_POP7_BDTv3.mobSeqPop7z#')+.0356679;>?>;60)$  #?b _:wncWK>2'  *?Vp "9OdwyeP:%~wrolhd_[WVWY^djpuy|||zywvutsssux}5PlqP0p_VU[eovxwrmjjp|HO)bu:TU" &;GH?0xtrrrrqqpnjd\RH@<?GVj X% XCrju}Q803=HRY]`fpV3P\U;VlZNGB><;:;;<<<=>@DHMRTTQLFA>>??<4((17985310.,)&#  !%+6AJLE6# &-/-*(),/110../12/("%$  !!!!#&),0369;=?BEHJKJHD>7/& *K}T2i?8U(o.l>)$'/7?FLPSTSROJE@;62.+)'&%$#" ",?_ZeV1#+17;=?@@>;6/(! 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ZG{M-K >-r@)KphG4`( jY9IMw Pkfe?`e1]o?#I%5Sd7_)PcXDlX`)RK=|fY~{ka xv.T|NL v TbBW$axy1dcj^ @wm3?$N~%Q ?$"N0M8^Na>!tH) L^InWYyiWboY6{NU73TZr6N r[iO 1_$> u ~ak UEf3 N!< < [} q7xJ W 9z 0h   (u) `X WoxM_qq_LPF@XXK73x/Axx50/U" !("   x ,      .|J? ;"Lp#S6p";PL;.OpQBw&OY[XBCk Q}5B{\l&[O+^{&@i6ib /Cy|%hSqLtJNC^LFF!b I @BhdJXaiD<!Zd|1E @nPCKJj=!_ZR,Md/Y(52u HqFedy_Y!5 02Xrd=[?dD;.% Dp%*C BxQp{p]R ~s @IKkWLB!+Khvi4Q) %l1&a6:E% C ! +-.$%   )'4&< &$ &"& #( &!"$ ) *2    !!%' 5    #  E"%'"%Y5)D K!. 5 9 1 &&'(!7#0' &8!D %'!    % A)G$ ?*&&+#&#Sa*2#%.0'$9''2"5-14(a#%p=>SE>B):6 <O:TAGATCGACCTAACACAATCGCGCTAGCGCGCTACCAATACCGACATAGGCCACCTCCTGACCTAACATATTAGCGCCCCATGAACGGTGGAAGAACTATTGCTTTCTCATAAAAGATTTAGAAAGGAGCCGGCGCTTCACCACATAAATTAGGGGAGGCGGCGTACATAAACTCCACGGGTATGTCGAACTCAAGATCGCAAGCTGCTCGAAGCTCGTGGGAAAGGAGCAGACACAAAGGGAAGAGCAACCTCGAAACTATACTGCAGATTAACCTTGGGATAAACTAAGGTACCATACAGTGGAAACTGTCGGGTAGAGAGGGGGAAGACTACGCCTCCTCAATACTAAACAGAACGGAGGTACGGCTACGATTTAGGGATGCTCTTCCCCGATGTCCGGAAAGTTTGCTAGTATTATTCGATATTATTCTTTTTTTTCAGAACGAGTCGACCAGATCCAATGAGCAGAGCAAGGGGAATACCCAAAACCGAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-30 22:23:45 -04:00AEPt?tAEPt?tAPFN ZAPXV2APrN 6*APrV1APrX}}63ARTNASPFASPtLASPtLB1PtKB1PtKBCTS}GBufT OCMNT 5CTIDOCTNMOCTOwOCTTL 5CpEPDATA@DATA@DATA@DATA@DATA DATA DATA !DATA +cDATA ?4Z DATA ?4=DATA ?4XqDATA ?4ץDCHTDSamDySN 5Dye#DyeN5DyeN5DyeN5DyeN5DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 5YEVNT 5eEVNT5qEVNT5FWO_GATCGTyp!!5HCFGCEHCFGHCFGHCFG==InScInVtLANE+LAST~~{LIMS!!5LNTD2LsrPaMCHNMODF6MODL3730NAVGNLNE`NOISVOfSc IP1AMj`P1RLfP1WDn<P2AMuP2BAyP2RLrPBASYPBASWPCONdPCONbPDMFYPDMFVPLOC^PLOCZPSZE`PTYPOPXLBRGNmORMXV1RMdNGRMdV1RMdXGRPrNORPrV1RUND RUND RUND RUND RUNT $RUNT /RUNT RUNT Rate 5RunN""OS/N%VSCAN@SMED SMLtSMPL YSPACAbֹSPACVSPACAbֹSVER4.0SVER }<SVER,,5-SatdScal@Scan@TUBEC5Tmpr<UserphARphCHWphDYbigphQLcphTRphTR&߁This is the confidence range *ABI_Limits..FVocAEPt?tAEPt?tAPFN ZAPXV2APrN 6*APrV1APrX}}63ARTNASPFASPtLASPtLB1PtKB1PtKBCTS}GBufT OCMNT 5CTIDOCTNMOCTOwOCTTL 5CpEPDATA@DATA@DATA@DATA@DATA DATA DATA !DATA +cDATA ?4Z DATA ?4=DATA ?4XqDATA ?4ץDCHTDSamDySN 5Dye#DyeN5DyeN5DyeN5DyeN5DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 5YEVNT 5eEVNT5qEVNT5FTabFVocFWO_GATCFeat//bGTyp!!5HCFGCEHCFGHCFGHCFG==InScInVtLANE+LAST~~{LIMS!!5LNTD2LsrPaMCHNMODF6MODL3730NAVGNLNE`NOISVOfSc IP1AMj`P1RLfP1WDn<P2AMuP2BAyP2RLrPBASYPBASWPCONdPCONbPDMFYPDMFVPLOC^PLOCZPSZE`PTYPOPXLBRGNmORMXV1RMdNGRMdV1RMdXGRPrNORPrV1RUND RUND RUND RUND RUNT $RUNT /RUNT RUNT Rate 5RunN""OS/N%VSCAN@SMED SMLtSMPL YSPACAbֹSPACVSPACAbֹSVER4.0SVER }<SVER,,5-SatdScal@Scan@TUBEC5Tmpr<UserphARphCHWphDYbigphQLcphTRphTR~WhC0-#JpPJwiO*$]_`#?Z9rS}G|u8 ]qU(6ji,x5?h8]Z~m$F5:U~2C 4"])|JjJDeV2E+qmj4I73wJzO`P{ e,&o.]/+M;`8hrjuO*@NzRoB+ks9?OkZKUW59$DtVzs6KP8)y$uVi220b_'{TX@Ep1JdQeK~jcaog_lQFTlzqz^M4'Uw\Pcc{\A_}$A>AR5Z(b|U2mC!KC_KdrWS[jWcgaWmUbe\W'KFkaqQltX[3>>B~:^gWiD!}%`;pT\m34yHf9MBEfIH(cO9+!!f|bEdGn~'_26"nlG;|>k0|3.B|uxPR~{z7_lnqR&GX>2Z <}EGCN8mgmktayr=uzF4/T(zNpP S!oSR5*6Y?_MBSmv^LoT.i6ti#O7(V6[/3-6)SE8pT/@MOsijd(DUfbLbB.!}^Ad}u2CvpRCu&WTu`Gn'zikr%/N2fKB_,qz7.5k|_MBSmv^>:.<)%,8EE.Om,P>bH6X128)TK7\4v3s'kY6C}B-mq5FaT2V3pVoqCH-p@v\vhsW__Z';fQGE-`x&iJ-,qzjN"o+Ax8amb,.63<%Ri24WF=-U[D~TWY+N@c'v+gK >o>s1H#"$w8@&!]T68n(0Ju*zv&1M2F{H/`WajZUTw|INi@C,6KP8)s5AVihx_7&OMb=#w&bN"@7hhSc%bVm? 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