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***>>>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR(`tz{{zzzxxwwvtrqqonljigedb_]\YXVTRPNMNNFFFcccyyy   ''(0/0877???FFKMMTTTZZZaaagggnmmssryyy~}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: c170f83e36b74b878d2073e2304c6bdbSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-30_20-36_07003130Seq16744134747_134747mfagan96-WellK1316_C3_Indeterminate_sp_psbA3KB_3730_POP7_BDTv3.mobSeqPop7ziiiijjjjihggfffffffeca`^]]]\\[ZYXWVVVUUTSRRRQQQQQPPOONOOQRSTUUVXY[\[XRI>2&  #1>GMQU[er"')(% reZQHA:3.)&%%&'()))*,.157:;=>@ACDDEEGIMS[er"9M^jqsncQ:{nc[UOKGC?:62/--1:H_}UL;quAW[dPC<:<@FOZhzLC^*x- 8ZjdD i On]\iL g*eI6+$"!"%*2?Rl<hk;yX?-!       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I!""1^%~ %<,6:I : !%$.(^4l# '"$' =+D3/aK*-n"#%T~T$8lm'!<2<B5AaF0G> $U?(.V) 4 D]y3?5ER}9N"G09z#'&#S8N(ZeD- .m67b(;Z0)"ING=' 0,O@,8Y$ %4,n;35'+; 09S~3n}.O%Nd25g8!."(--"U`90QjZ[=#co+D#4NZ.Nb;6fes:#'5[hA2IP=F%9%KG&\(KNJC1N"?mUHn9/\l1+&W9Q2uGNZ1-"&.&##tYkOU532/{J2F&8EaACgsi0qM}EAk^F}K#QLJ<'PG$:}3NcE1.@AO):;p-9Bgp.<_DM\9fy/<0r zp.z2j<;Y76G x PP\OX S}pzATAAACTTCTAACAATAGGTGGTCGAGAGCTACCTATATGTATATAGGACACCTCCGTCCTTATATACGCGACCACGAACTCACCTTCTTAGCGGGGCCCTCCCCCTTTTTTCTGTCCCAGAGGGACTTCCTCCATCCCGATACCATCATGGACTAGCTCGAGCTTCCACCACTCAACTAAATATCAGGAGCATTACCTATCTCCCCCAGCCATAGGGATATGTGACCACAACACAGGCAGAGGGGGGAGAAGACCCTCCATGGTATATTTCTAAAATGGAGTGTCTTCTCCCCTCGGGCCGCTCTCCAAAAAAATGGCTGCCTCAGAAGGGTATATCTCTAAAATATGTTCCTACCAAGGAAAGACCACATTCTCCTCTCCATCCAAGGAAATGTGTAGGACCAAAATATAAAGGGGAGGTTAGATCTCTCGAATAAAAATAAAGCAAAAGGAGCACAAGCCCAGTCCCGTAATCCCGGAAAAGGAGCGGGTTCTCTCCCACCCTTTAGGTGTTGAAGGACCCTTTATTCGCGGTAATTGGAATCCCGGGGCCTCCGATAAATGATCCCACCCTTTTATAGAAGGGAATACTCCCCGCCTAAGTTTTCCAGTAGGTCTACACCGTTGAATGAGTTCAAAAACGAACACAAAGGACCAAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-30 22:23:27 -04:00AEPt@AEPt@APFN VAPXV2APrN 3APrV1APrX}}3ARTNASPFASPtMASPtMB1PtLB1PtLBCTSBufT LCMNT 2CTIDLCTNMLCTOwLCTTL 2CpEPDATA@DATA@DATA@DATA@DATA  DATA DATA DATA (KDATA ?ZVDATA ?ZKDATA ?ZUDATA ?ZDCHTDSamDySN 2Dye#DyeN2DyeN2DyeN2DyeN2DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 2AEVNT 2MEVNT2YEVNT2lFWO_GATCGTyp!!2HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!2LNTD2LsrPaMCHNMODF3MODL3730NAVGNLNE`NOISTaOfScL0 P1AM(n\P1RL(i4P1WD(sP2AM(}P2BAP2RL(xPBASW(PBASTPCONfPCONd PDMFVPDMFTxPLOC(^PLOC(YPSZE`PTYPLPXLBRGNmLRMXV1RMdNDRMdV1RMdXDRPrNLRPrV1RUND RUND RUND RUND RUNT $RUNT /RUNT RUNT Rate 2RunN""LS/N%TYSCAN@SMED SMLtSMPL VSPACAdSPACTqSPACAdSVER4.0SVER SVER,,2SatdL0Scal@Scan@TUBEC3Tmpr<UserphARphCHTphDYbigphQLcphTRphTRzThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN VAPXV2APrN 3APrV1APrX}}3ARTNASPFASPtMASPtMB1PtLB1PtLBCTSBufT LCMNT 2CTIDLCTNMLCTOwLCTTL 2CpEPDATA@DATA@DATA@DATA@DATA  DATA DATA DATA (KDATA ?ZVDATA ?ZKDATA ?ZUDATA ?ZDCHTDSamDySN 2Dye#DyeN2DyeN2DyeN2DyeN2DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 2AEVNT 2MEVNT2YEVNT2lFTabtFVoccFWO_GATCFeat//4GTyp!!2HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!2LNTD2LsrPaMCHNMODF3MODL3730NAVGNLNE`NOISTaOfScL0 P1AM(n\P1RL(i4P1WD(sP2AM(}P2BAP2RL(xPBASW(PBASTPCONfPCONd PDMFVPDMFTxPLOC(^PLOC(YPSZE`PTYPLPXLBRGNmLRMXV1RMdNDRMdV1RMdXDRPrNLRPrV1RUND RUND RUND RUND RUNT $RUNT /RUNT RUNT Rate 2RunN""LS/N%TYSCAN@SMED SMLtSMPL VSPACAdSPACTqSPACAdSVER4.0SVER SVER,,2SatdL0Scal@Scan@TUBEC3Tmpr<UserphARphCHTphDYbigphQLcphTRphTR]nd3ghlc T-* +Wt3z{0bInTor@tbfD7E)0Ie/AK8N'nsv&'Y?sxWlZN.YRz!#fn2%FT!sz^2h3)-I5d~)Uk}`:r&xvr_WG\|8 Xo2h{/S9~i,EAx8amb#QJU0iHAj}W2yv!a!<}B]rQHfKbVU}Uz1p=|(LZjp7xMO9j;q6TB(2JLvuUlN1@#vQ!TMF4FprjDp*K0.]+0Oj!Q4aK{WlVO:}mldKcL Z#nHW{$~y!.]t5+y|`}__';_/A@(#jFnjUZ'KvNIGQ5ENI@[wQi^*w% enqb[*jG3T%ycX}%z/q1wv;{?kJ:hAqTFU-vbNhsrU5-uvCbbJ_<$AJu"#ph`*d+S*pF+{d&J9Z&Y?XYoF-Tk>.0Nb0E%L(!f|bEdGKI'_26.?bFW_E7bp~4o-D7I 9_[*?bCDkrD5pY^d,/IQ}{sGitGKXW .CTNCwD(ftyYZFUI8-W=;OF>BrRL8~f)NKo-bEd9gP`gvG9 b?AvQtb'J!>yH~~!|s&4)D@xG57'9\47.<<_r[)PCy5"igxd}s9?Od+k'"5-z]G3o6Rl6%4y^.EAx8aPI^QJH){!9Y2]2f1hu?K2FtSEH$*YYo%@rs{6`VsIeJM*{B]9[ikI0o6Gzv&1`Z=kY+-^tG|JWoa-s1tob*oCfvn}4|Md'ac_` WV|N&WM{7r iwH k:8`[On.e{:^kkSOj!Q4KK*flVy8J9tGHrd+"L&d&2<\d&k@6GZ]]C)TiSOpP S!oS{Rn[-Vt2`\wmj7ar?U)P? sIRD8z{KJ[.z^`A(uKN73B!Hiw8X&L>o.B3@dSO/qg616B4#ky`[qDr_WG\|[S*.JJof6[[HhEg5{og]|_}e])%O1"@7hhSc%bVm? : wT_RuhGlB^PY6C}Afz@jXaMuF_QJO!GE#!#hmmfc4.=+I}S$oa$fU~oM6CqwToN668d#