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'(54MFW]Q6QG+0' &"  !!              *&&"9=# , 'CN[CD& $ '%  +% ("         #1*%10$                 !#( )                       )$(&31./#%0- &')"%- &""         cdefghijklmnopqrstuvwxyz{|}~cdefghijklmnopqrstuvwxyz{|}~,,@II^^^{{{&&&999DDDXXXaaavv      ***>>>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR(`tz{{zzzxxwwvtrqqonljigedb_]\YXVTRPNMNNFFFcccyyy   ''(0/0877???FFKMMTTTZZZaaagggnmmssryyy~}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 022f74b676f34c0cbfd93a90ea52f92bSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-30_20-36_07003130Seq16744134747_134747mfagan96-WellK1316_C2_Indeterminate_sp_psbA3KB_3730_POP7_BDTv3.mobSeqPop7z+=MZenw#'*(#yrCOM} ]qIu95(LhM3 ?yF|?n "./&Y%N!vmhdccegimrw 2Mo=|!BSUH/ ~D iB$ {wxK;qs[L!#v? $<ShyyV2}rljjloqrtwy{}|{{:e<3^2hZUUXZ[[YZ]h|O>~v5Lv_PHDA?=;::;<=>>?@CGMT[chkkg`VK@6-&!  %M.V,QX- %+04651)  +sqjgG7b!:@C-HHC}wx|rB~Q?@IT]`^XOD:2,))*,.//,'  :pU2Z Ph=F^$/, !###!    6y+'jfGtA^H   #M2Yk736%&-3:?BA=5,"  %**("   &Q;ODe4,]]LC     '263)C1~<+:v( &6BGD;-   )29<<93.($  )<LWZUH7$%/43.$ )4::4+    #dPcd`Pd\\*")D, Aa :m.     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n dO Z pZ8!%%l[]2  A:4 XAw(^vRuQ G0Anb!RC aGha4X  I2:E 5 DZ= ig V  \LV'C n{ltfC~]$;M=5.@m,@:&&/5   !    7:)\3Ob?'$GPH5 24. )J ,I. *;*Z /8%&S2@ }*WhBKQ?(x\)A<Bd pps.9pwu|&p h jJ9[ptB&8]-I7,k=`\'o~5e5J;cUugz.=iK$KJm}MYL~\lcHyN:<;O8Y{PC%)\o`f_3m0 /i&y.yI+Lkz &l /9^Ud.v |WDZvyq~l79bfh= [g"d"T,:f'I| _v?Jdz v7MyC ;#58{[vg4op }p C V f B8{KYwvSFYeEm:h=QHU=qQw_{Uk[d//U_SG51hd%B4{Zm/3V+N@WAEK_E/ic%q90,5+<J7{"b%R{>V#Q SW'N.QEBb^z(4/nEJC_4G FgA(9;@DOh~`P36BgO~>!cNr^e &0&-=ovs1gg@ TYI7hY.gR)#aCW>m8-`&cQ0@&/'#c"d+@ZrY9m\1IqG"L-\ES3RE`qb mRazpm/q<xe&w`a-k,x. uvtS4 8 M0(?ORNB/ `6u2ZraDkh5l0J;6! 5c e#Ed]LAAAAAACCCCTAACAATAGGCGATAGAGAGCTACCTACACTTAATTGGTATGACCTTAACATAAAGTAGATCAAACACTTTGGGGTACGTCCGCAATAGCCGTATCTCTTCAAAAGATTCCGTTACTTACAAAGGGATACTAACCAAAATCCCGTTCCACCAAGAGTATGGGGCTTGTCCCATATTTTAGTTCTTCCAAAAGCGCGCCACCCCTGAGACCCAAGCCCTACCCATGTGTAGCTGGGACTTCAATATAACCTGGGGCAATGGGGGAAAGTCATGAGAATGTGATTGACTCAAAAGAAGGGTCCCATCCCGCCGAAAAAGGGCCAATATAAATACCTCTAGTGTATAAAAACGCCCACTGCATGCCAACGAGTTAAGTGTGCTTTTCCCCCGCTTCAACCAACGCCAGCAGTTGTTTCTTTTTTTTTAGCGGAGTGATTTTTGACGGTTTGACCCACTTAATGATTCAATACCAACTATGGCCGAAATTTTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-30 22:23:12 -04:00AEPt=AEPt=APFN UAPXV2APrN 1APrV1APrX}}1ARTNASPFASPtMASPtMB1PtLB1PtLBCTSqKBufT KCMNT 1CTIDKCTNMKCTOwKCTTL 1CpEPDATA@DATA@DATA@DATA@DATA  DATA DATA YDATA '#DATA >}2UDATA >}2DATA >}2P-DATA >}2_DCHTDSamDySN 1Dye#DyeN1DyeN1DyeN1DyeN1DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 1EVNT 1%EVNT11EVNT1DFWO_GATCGTyp!!1cHCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~oLIMS!!1LNTD2LsrPaMCHNMODF1MODL3730NAVGNLNE`NOISJOfSc' )P1AM^@P1RLZ\P1WDb$P2AMiP2BAmP2RLfPBASLPBASJPCONXjPCONVxPDMFUPDMFJPLOCRPLOCNPSZE`PTYPKPXLBRGNmKRMXV1RMdNCpRMdV1RMdXCRPrNKbRPrV1RUND RUND RUND RUND RUNT $RUNT /RUNT RUNT Rate 1WRunN""KrS/N%JSCAN@SMED SMLtSMPL USPACAa|SPACJSPACAa|SVER4.0SVER q@SVER,,0Satd'Scal@Scan@TUBEC2Tmpr<UserphARphCHJphDYbigphQLcphTRphTR+This is the confidence range *ABI_Limits..FVocAEPt=AEPt=APFN UAPXV2APrN 1APrV1APrX}}1ARTNASPFASPtMASPtMB1PtLB1PtLBCTSqKBufT KCMNT 1CTIDKCTNMKCTOwKCTTL 1CpEPDATA@DATA@DATA@DATA@DATA  DATA DATA YDATA '#DATA >}2UDATA >}2DATA >}2P-DATA >}2_DCHTDSamDySN 1Dye#DyeN1DyeN1DyeN1DyeN1DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 1EVNT 1%EVNT11EVNT1DFTabFVocFWO_GATCFeat//fGTyp!!1cHCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~oLIMS!!1LNTD2LsrPaMCHNMODF1MODL3730NAVGNLNE`NOISJOfSc' )P1AM^@P1RLZ\P1WDb$P2AMiP2BAmP2RLfPBASLPBASJPCONXjPCONVxPDMFUPDMFJPLOCRPLOCNPSZE`PTYPKPXLBRGNmKRMXV1RMdNCpRMdV1RMdXCRPrNKbRPrV1RUND RUND RUND RUND RUNT $RUNT /RUNT RUNT Rate 1WRunN""KrS/N%JSCAN@SMED SMLtSMPL USPACAa|SPACJSPACAa|SVER4.0SVER q@SVER,,0Satd'Scal@Scan@TUBEC2Tmpr<UserphARphCHJphDYbigphQLcphTRphTRkl{Zq\-4/CeG1{?{sb: MOsh9VMR5t(jICx@VFmYK3AQZ`K'H:{GKipM`{`3e&Vc:z x:H-p9bgh`U*{Ga$FwXAr:3%6AB$AvG9 b?$o0)]j,XXL(y?<&jb!a72BZ|7.N[2MM@Z#bv]Z`lbkF3(jY`VqQIxwcL6-*pfFUeiM/+U*yxT/4f!Z56N'?k?~pmxRVQQ/^idT@CzS[~k8N'O?6pkC:J|bU,iPis|KRaD678w!lVcr,,z^qXnF){3&H9n9f}~E\\&(6Emc9tp] j MfaqQlYm.bBT9zH'zeE^O{U,\)lOY%}WeoVL6I-I#(0+5hf,MA:?LB++/CW22Q&]Q#V>Ax7cXF$~y;|>EY|3.B|ux).u7z"$-m8)FTk2XS"j+OC5wz^`Mv,ZD-_p;s_|g+V3SKo)67:I1@ -Tr+ TW>qO9`}!&RW8X&tG98PbC.Ju5%2-*H$I4f"ZKZ;| |G|-`T #o$K2LF%c{_'`gI2}__';_/Ft(#jFO-U[d1DFW%j'k0WC.%oKO> `VsIRD8z{!J!OpZIBoz?&qN^Fu*t":Ey+D;UTw|]jp&;2isfMFoM/V/5`agv`k';_szT/0p/(ZU&g"=6W\!3[s?RJAQ>KN73o ZbCd]ipPv6~MN#tH*J*q~|H~?pVRI<672tX>PG"BXP2gXk0[+D:rF(5,fc-,RIlVinJilZbCd]*>ZEx$DB+c7T-O)\rS|5qk`T1jdRC WIFM/|RB"H2gQaURkt;"W#G:jmj!>VHr%S!C*}=[^tHF@LeP *">BZuGZ-p@vddD\4ApZTFS$oak"T'oA_AN;)oX6YMI){mnjH[F.A[s:>ZCCd+4k,Z)lO[vHds<-F3x[R2LxC{IF}oH,k#_'>XA[.VJz$*#sejp~DsK2FtSEH$*YYo%rF(5,fX!Hiwe2{sGiSVqrj/.3~V'z(Z=kT/n@6TdO{7>kOptpuze/WR/X2EtERPV]O8oe])%,8Ea-cZ,f*F8B,}&8)TK7BKv'xPoQ`OY}]66{qT[sEF($}v4"/VAEPt=AEPt=APFN UAPXV2APrN 1APrV1APrX}}1ARTNASPFASPtMASPtMAUDTyB1PtLB1PtLBCTSqKBufT KCMNT 1CTIDKCTNMKCTOwKCTTL 1CpEPDATA@DATA@DATA@DATA@DATA  DATA DATA YDATA '#DATA >}2UDATA >}2DATA >}2P-DATA >}2_DCHTDSamDySN 1Dye#DyeN1DyeN1DyeN1DyeN1DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 1EVNT 1%EVNT11EVNT1DFTabFVocFWO_GATCFeat//fGTyp!!1cHCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~oLIMS!!1LNTD2LsrPaMCHNMODF1MODL3730NAVGNLNE`NOISJOfSc' )P1AM^@P1RLZ\P1WDb$P2AMiP2BAmP2RLfPBASLPBASJPCONXjPCONVxPDMFUPDMFJPLOCRPLOCNPSZE`PTYPKPXLBRGNmKRMXV1RMdNCpRMdV1RMdXCRPrNKbRPrV1RUND RUND RUND RUND RUNT $RUNT /RUNT RUNT Rate 1WRunN""KrS/N%JSCAN@SMED SMLtSMPL USPACAa|SPACJSPACAa|SVER4.0SVER q@SVER,,0Satd'Scal@Scan@TUBEC2Tmpr<UserphARphCHJphDYbigphQLcphTRphTR