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'                     defghijklmnopqrstuvwxyz{|}~HIJKLMNyz{|}~ Y Z [ \ ] ^ _ E defghijklmnopqrstuvwxyz{|}~HIJKLMNyz{|}~ Y Z [ \ ] ^ _ E ,,@II^^^{{{&&&999DDDXXXaaavv      ***>>>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR(`tz{{zzzxxwwvtrqqonljigedb_]\YXVTRPNMNNFFFcccyyy   ''(0/0877???FFKMMTTTZZZaaagggnmmssryyy~}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 3c2a3b6442b54b5389019a1bcef89a59Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-30_20-36_07003130Seq16744134747_134747mfagan96-WellK1316_A2_Vismia_baccifera_psbA3KB_3730_POP7_BDTv3.mobSeqPop7zQRSUWYZ[\]`dhklkjjknpojaVOO\w6e$B\p}udN3tB sM00Gc 6\yq_I1{oe\UNIFDCCDDDDEFGHJJJIHIIJKLKIGEDBA@@@ADHNU_k|,[5Um|~t\9 o; }i[QKHFFFGHJOWdw#m&n*lZE^5wfZRKFB@@@ABA?<:766789;<==<<;;<=>ADGIJKKMPXdv C+OceS/w2nYMGFFGIJKJHFFJUjz"Ara%}R;226<@DGHHFB=8557871' %)(%! 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E(Ya?a)  (Wi8 Gj8 )XY,  GO%  ?}1:b0 9oE "R_3   ;ki;       'N|uK(     %N\3    "Fs[5 =m_8    4SsiK/   !>_}y\>%   "<Xqv]@'0R{yT4     #;TjvwmYA* '?WisqdP9$     )SmX]zi? 5]rT@8:AHMOOPQTX[\[YVSOKF@:2+$ #/?Pbss`L9* &9Rm~cI2"     5# v@A*0AEOA=KB.bcpKB_3730_POP7_BDTv3.mobtermACTAGTACTGGATCTAGCTGCTATAGATGTCCCATCTATAAATGGATAACACCTTCGTGTTATTGTATAGGAGTTATTGAAAAATAAAGTAGCAATAAGGACCTCTTATTGCTACTTTATTTTATTTTAAGTTTTTTCCTTTTTTAAAATGAGTTTATTCTGCTATTTCATTTAGTATTCTATATATTATTTTATTAATATTATTTTATTATTTAGTATTTATATTTATTTTATTATTTAGTATTTATATTTAATTTCTGTTATTTCTATTTTTATATTTAGGTTAACATTTATACTACTATATTACTAGACTACTAGTCTACCTAAATCGATAATTTTTTTATTTTAATAGTTGTTAAAGAAAGGAACATGTTCGAACTCGAAGTTTATGAATGAAATATTAAAAATTAGAGCAGGGGGCGGATGTGGCCAAGTGGATCAAGGCAGTGGATTGTGAATCCACCCATGCGCGAGACTAGTACTGGATCTAGCTGCTATAGATGTCCCATCTATAAATGGATAACACCTTCGTGTTATTGTATAGGAGTTATTGAAAAATAAAGTAGCAATAAGGACCTCTTATTGCTACTTTATTTTATTTTAAGTTTTTTCCTTTTTTAAAATGAGTTTATTCTGCTATTTCATTTAGTATTCTATATATTATTTTATTAATATTATTTTATTATTTAGTATTTATATTTATTTTATTATTTAGTATTTATATTTAATTTCTGTTATTTCTATTTTTATATTTAGGTTAACATTTATACTACTATATTACTAGACTACTAGTCTACCTAAATCGATAATTTTTTTATTTTAATAGTTGTTAAAGAAAGGAACATGTTCGAACTCGAAGTTTATGAATGAAATATTAAAAATTAGAGCAGGGGGCGGATGTGGCCAAGTGGATCAAGGCAGTGGATTGTGAATCCACCCATGCGCGAG!0<FN^jo*8AP]ht~#/:ENZgt *8FR^iu +8DOYep| $0;ER^ku $0<HT_kx'2=IVbo{ !,9EQ\hs    & 1 = J U a n z  ! . 9 D O [ h u    ( 3 > K W b n y  # . : F R _ j u    * 5 @ L W c p }  $0=HUbnz #/;GTalx +7CP\hu'3?LXeq~ %2=IUbmy ,8DP\hu*7CP]gs'3?JT_iqx!0<FN^jo*8AP]ht~#/:ENZgt *8FR^iu +8DOYep| $0;ER^ku $0<HT_kx'2=IVbo{ !,9EQ\hs    & 1 = J U a n z  ! . 9 D O [ h u    ( 3 > K W b n y  # . : F R _ j u    * 5 @ L W c p }  $0=HUbnz #/;GTalx +7CP\hu'3?LXeq~ %2=IUbmy ,8DP\hu*7CP]gs'3?JT_iqx  -' *'2  )/28*>3)>>>>$+>>>%!8;>>>;;>6>6>6>>>>>>>;>;>>>>>;>>>;>>>>>>>>>>>;;>6>>>>>>>>;>>>>;>>>66>>6>;>;>>>>6666616>>>;>>>>>>>>>>>>;>>66>6>>>>6;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>66>>>>>>)"'">>>>>>>>>>>>>>>>>>>;;>>>>>>>>>>>>>>>>>>>>>>66>>>6166>>>>>>>>>>>*>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>6>>>>>>>>+>>>>>>6>>>>6>6>>>;>>>>>>>>>>>>>>>>>>>>>>>;>>>>>3>>>>>>>>>>>>>>>>>>>11>>11>>>>>>>>;>>>>>>>;+-" >8&,%8>8*$//8>8.(2$2 *    -' *'2  )/28*>3)>>>>$+>>>%!8;>>>;;>6>6>6>>>>>>>;>;>>>>>;>>>;>>>>>>>>>>>;;>6>>>>>>>>;>>>>;>>>66>>6>;>;>>>>6666616>>>;>>>>>>>>>>>>;>>66>6>>>>6;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>66>>>>>>)"'">>>>>>>>>>>>>>>>>>>;;>>>>>>>>>>>>>>>>>>>>>>66>>>6166>>>>>>>>>>>*>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>6>>>>>>>>+>>>>>>6>>>>6>6>>>;>>>>>>>>>>>>>>>>>>>>>>>;>>>>>3>>>>>>>>>>>>>>>>>>>11>>11>>>>>>>>;>>>>>>>;+-" >8&,%8>8*$//8>8.(2$2 *  8:6?+W6GAU\>B=0Zc[Va^LEOMG`D) 70LGE<LO;0EH:@> GFaHN[ E`TO"YQD8:A4EX :&= G(6P$(1!5RXJPO +&P#`_WH>=<-K:I3*FaV$35*Z>V&&F/!V+]3>MIAD\EUB\KEG1VN$L*0%E @$%YEb_@@ZQ? Y<[8 Q0ITH ?Y9>6@M%b<YXP8NN H!56HAY8b+Y*GE8Q/]='.a/. >265GBUI3^$I`%?879*F;&_63O+F@?bL8 -Y,(H2L P:=&a 4C%U#OR-T#^J73<P0VS_W`:X'^W[[X\2K7C$-b6!SC [^RC.7S %IW<& -`)/=)FWK540420BOH22$%2 dk6l Qt0*}N91!ui@AE ioQ\ax8c:X[~z?3LmAI-}@(ADT!Q{Yb(\<p?<%POi+PON-OokhO^0xw".5J6m!/f(^wn6X0@"6fW`mB:eN hWW6* ,R/<r%)$+Hq9]{JSoG.chA1+)3P%v"'-k&mR.v'>80Q_ rMMU#R8SE{)bHKlxpo@c&*[8eaBd ~Iw[cbd M hh\ H C T{q:/4@@p@q@@Td}ZWH^F}WI@OK,x(*,3*N !  x( 1  @pL0pvpK gev=&! pZ4bbs":{j"cF`*\<~}I[8?{"(KY7WW$8jdl`?gDr ' }pdf1d_U558T]"p8{Y*%F`d!y/D1e)D'J 88TdAi0!KH $?<V4B4A3A$ EOED\ !Q 8;^bHKp9n=9/jC?;G] l1Zg  YUYz5+vkk4<P5$5NEw@'v.BCE+1' $J? #&   ,+D=##$ ;    PM' &""$ ,g&&F C %P &"  !- %,-"$&&* A!($&Q* .(0\     /!  !  *3&")!#")7 $08$ D(&6!-3%!/.K$#856D'%! / QB681-6 6<=(A-S&=>4* +93.3  )!&#%%!F.%,"330UB<h>#SF;.V;@92t_'(fHgF]"5ug}CAACCCCAAAATATATCGGCGATATATATGATACCTATATGTAAATATCACGTCCACGTCATAACGTAGAAGAGATACTGGGGTACGGAGGAGCGAGGAACATCTCCCGGCGCCACAATGAAATAAGACTAACCCACTTCCCAAATTTGAAGTGAATCCTCCGCGGCATCGCACAACAAACCCCCGCCGAAACTAACGAGAATGCGAGCCGAAGGAAGGCGGCCAAGGAACGGGGGACCCCGGGGGGGGGAAGGAAATCCGGGGGATCGCCAACGGGAAAGCCGGGGTCAACTCTTCGTCGACAAGGCGAGACTTCCCATCTTTCCCGGTAGCGTAAAAGGGTCCAATTGGAAACGGGTTAGGGAAGGGCCCCGTTCCAATCCCAAAGTGTTTAATGGATAATTTGGAAGTGATCCCATTGCATCTATTTTTTTCAAGAGGTTAAGAGCTGGGAATTGGGACATACACAAGAGABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-30 22:23:16 -04:00AEPt@pAEPt@pAPFN W@APXV2APrN 3jAPrV1APrX}}3sARTNASPFASPtYASPtYB1PtXB1PtXBCTSyBufT M?CMNT 3,CTIDMCTNMM"CTOwM0CTTL 3#CpEPDATA@DATA@DATA@DATA@DATA  EDATA DATA DATA (DATA ?TWIDATA ?T֝DATA ?TUDATA ?TEDCHTDSamDySN 3Dye#DyeN3DyeN3DyeN3DyeN3DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 2EVNT 2EVNT2EVNT2FWO_GATCGTyp!!2HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~xLIMS!!39LNTD2LsrPaMCHNMODF3ZMODL3730NAVGNLNE`NOISTOfScW\ P1AMgXP1RLcP1WDk P2AMrtP2BAv(P2RLnPBASVPBASTPCONaPCON_PDMFW)PDMFTPLOC\<PLOCXPSZE`PTYPM7PXLBRGNmMRMXV1RMdNDRMdV1RMdXERPrNLRPrV1RUND RUND RUND RUND RUNT $RUNT /RUNT RUNT Rate 2RunN""LS/N%TSCAN@SMED SMLtSMPL W SPACAgjSPACTSPACAgjSVER4.0SVER ySVER,,2mSatdW\Scal@Scan@TUBEA2Tmpr<UserphARphCHTphDYbigphQLcphTRphTRҁThis is the confidence range *ABI_Limits..FVocAEPt@pAEPt@pAPFN W@APXV2APrN 3jAPrV1APrX}}3sARTNASPFASPtYASPtYB1PtXB1PtXBCTSyBufT M?CMNT 3,CTIDMCTNMM"CTOwM0CTTL 3#CpEPDATA@DATA@DATA@DATA@DATA  EDATA DATA DATA (DATA ?TWIDATA ?T֝DATA ?TUDATA ?TEDCHTDSamDySN 3Dye#DyeN3DyeN3DyeN3DyeN3DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 2EVNT 2EVNT2EVNT2FTabFVocFWO_GATCFeat//GTyp!!2HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~xLIMS!!39LNTD2LsrPaMCHNMODF3ZMODL3730NAVGNLNE`NOISTOfScW\ P1AMgXP1RLcP1WDk P2AMrtP2BAv(P2RLnPBASVPBASTPCONaPCON_PDMFW)PDMFTPLOC\<PLOCXPSZE`PTYPM7PXLBRGNmMRMXV1RMdNDRMdV1RMdXERPrNLRPrV1RUND RUND RUND RUND RUNT $RUNT /RUNT RUNT Rate 2RunN""LS/N%TSCAN@SMED SMLtSMPL W SPACAgjSPACTSPACAgjSVER4.0SVER ySVER,,2mSatdW\Scal@Scan@TUBEA2Tmpr<UserphARphCHTphDYbigphQLcphTRphTR4{,|Qv%F}K7T]Zj7y!R}|MCZ9zvr3H}T"mqD-WeDmq$a~8;R/;8-b#DI#k>4L\\W'KFxz2jA&owbor~Mu^yu6v;(vJ4;iD4ZI,nG9T+8@/Sco6m~2HBII]#'LygURm}Tkl0ml{W7;rRU)>`V2]<|'kp?_sDk`457^^s?92>@iG-@,P> u)v/|vu~CM#Y\Vd*U5aO/CsINi@C,{s2fo;LY&S2Bjr<|Ate1}1"/"@-bWF4oFo;X.: wT_fuwQEnfD~VJ(/on}sF{AR8=(V-OGC#!^MrrG]iQULBGOnie-u23_=LDq;I#Ml(NuDu`($AhVKubQ2a5NDzU4oFo;X.u8xXIc62nrD5pY(d+{o.jaFsA bXX<,x'EEwiOfF{\)lOY1|YL0eF`x&iJ-A|K7~57|NxOrz6 ?63_iPQ2aLtyc@"SD=;LX@HgX63g4`m^*Q1V'.$b[2$YWmOyl}$rEGCN8V+lwc7*<&YTIu{Qqk+^tpu"pcSIoM/V/5`agvLAX;:,aXj`Q Dx6m}aD&Wr`sEHu]4kkSnwkI6 +FwMz%FT!sz^`A(O>|ESRDujXTE-yh;}S$oa$N;172e,qz7|!y]18`'|_n?26.?bFWJQ:Ocl2[ALYNBLnTMg:(qR:!iuyxFlPBK/axJK[YlyjSu3]aC^DfF<7:cmj^sSU|MkO_b2(_CL,vnQ6?ZdwYc_`LJ6txWTikt72WevdAV|+ow,6Q)2Zjpbb-y]2|>xAC3w[0 dV#JX;MM;8'i2{q6.>`A #pJE@^*\vJnPk=o?eU:g;TaywK[*oCf`y6n?2x`agv`k=;A{%i7#W yZE!e~8WY;`.pr$,.[G57'9\47.e6bZ( iu2Zv&1`2h`_`&1hE;qT%;5]U'V[,A4#O|!faiw`6wPjgvG9 HtWo0U:6%4sIe0