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"($"1 !   %!      &&& +     %   /%                    "wxy~wxy~,,@II^^^{{{&&&999DDDXXXaaavv      ***>>>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR(`tz{{zzzxxwwvtrqqonljigedb_]\YXVTRPNMNNFFFcccyyy   ''(0/0877???FFKMMTTTZZZaaagggnmmssryyy~}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: f5c988d2ede44349bff458f11aa63ebcSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-06-30_20-36_07003130Seq16744134747_134747mfagan96-WellK1316_A10_Cupania_glabra_psbA3KB_3730_POP7_BDTv3.mobSeqPop7z+6@JRY^dks|}vpkgda^[XTOH>3( |pe[TMHD@=<<?CIQZes(@Wl|~jT<# ~n`SG=4-'$#$&*.38>DLT^gpy /WA$DZiom`H$pP:/,.4:>>8+b5l\OE=979?J[u V r;!FRAr 5|3yst{&TbP^Q=qcYQJC;3+#"-@]3}Oo1<zU=/)+19CLU^jyGjmIvW?-!"*29>@A?<71*"   )7CMSVURLE>82/,+*)($   (06<@CEGGGGGFC@<843359<>>;5,#  $''%!!&,29?CB=4(G_,2?ew+gz/-d5&.5:=<81)! 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'.353.&    %2<A@8,   !"! &-11,%  &2=EIG@5) )8IW__VG5# ",7@EFA8-")5@FHE<1$     )2;CHKIA6( $2>FGC;0&  ds`;L      +|~a~POQ)PUk6>   .8=<4(    AI9w/ 3<9,    "&&#   %s| sxx&  !$%"       "%&%!#&'%""! #'% ifKlDYb    "uRMdW>Q Q`IQe|x)                 "iR:;Z                      J&>+ct*G4F/2   C(h}K_(  "c2lB         \6) Bb        #e3V7          D@~@O!     =~ ]wg0H  $Z2UO$L      !S(PR/\'      2l9RB g' b+8<     %N  M#  .\V.         Z(YP|`u& -Mvzk_Y^o2@6Z>-&#  +9CGF@5* /R3RU;\<.-143-"4qf]tA$"(,,)%  !&(&" 7 @'AtATOAKB.bcpKB_3730_POP7_BDTv3.mobtermCCATGCATCTGACTGGCTGCTGTTGAGCTCCATATACAAATGGATAATGCTTCCTACTTATGTTAATGTTACTTATGTTAATGTTACTTATGTTAATGTATAGTAATTGTTGAAGGAGCAATACCAATTTCTTGTTTTTCAAGTTTTTGGTATTGCTTCCCGAATTTAGAATTTACTAGTGTTTTATTGATATTTTATTTACTCGGGCATCATTTTTTTAATCGTTTAGGTATACTTTTTTTTGTATTACTATAATACATATAGATTTAACTTAAGGGGCGGATGTAGCCAAGTGGATCAAGGCAGTGGATTGTGAATCCAGCATGCGCGAGCGCCATGCATCTGACTGGCTGCTGTTGAGCTCCATATACAAATGGATAATGCTTCCTACTTATGTTAATGTTACTTATGTTAATGTTACTTATGTTAATGTATAGTAATTGTTGAAGGAGCAATACCAATTTCTTGTTTTTCAAGTTTTTGGTATTGCTTCCCGAATTTAGAATTTACTAGTGTTTTATTGATATTTTATTTACTCGGGCATCATTTTTTTAATCGTTTAGGTATACTTTTTTTTGTATTACTATAATACATATAGATTTAACTTAAGGGGCGGATGTAGCCAAGTGGATCAAGGCAGTGGATTGTGAATCCAGCATGCGCGAGCG*4>H[cv)6CSbmy '0<FQ`lx  +6EP\gr~$1>ITbn{+7DQ]ht #0<HUamy*4AMYfr~ $0:FS^kx    , 8 C O [ h s ~    * 6 B N Y d p |  $ 1 > J V b n z    ) 5 A M Y f s     ) 6 C P Z f s ~ '4@MYdq}  1=IUcp}*4>H[cv)6CSbmy '0<FQ`lx  +6EP\gr~$1>ITbn{+7DQ]ht #0<HUamy*4AMYfr~ $0:FS^kx    , 8 C O [ h s ~    * 6 B N Y d p |  $ 1 > J V b n z    ) 5 A M Y f s     ) 6 C P Z f s ~ '4@MYdq}  1=IUcp}  ( !2,3)"4.4) ,4->688&/>88883>>>>>;;;;;;;;>;>>>>>6>>;331681;>;;>;>>;;>;;>;>;28>;;>>>>>>;;>>8;>>>>>>>>>>>>>>>>>>>>;>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>6;>>>>>>>>>>>>>>>>66>>6>>>>>8;>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>66>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>;>>>>;>>3>>>>>;>>>>>>+"0.;3;;>>28*+8#     ( !2,3)"4.4) ,4->688&/>88883>>>>>;;;;;;;;>;>>>>>6>>;331681;>;;>;>>;;>;;>;>;28>;;>>>>>>;;>>8;>>>>>>>>>>>>>>>>>>>>;>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>6;>>>>>>>>>>>>>>>>66>>6>>>>>8;>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>66>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>;>>>>;>>3>>>>>;>>>>>>+"0.;3;;>>28*+8#   : ._L=?UU%O) _^&YR0= LA"-ET/ aLVQ;T-0 d:C+!'VMZG1 V@1_ Id%,'J.W$$#d&0/)KbOHPbK.C7"+SR6E-L 2P-&[ &L6DC$ILLFIJQ+*F 3Z,b' VAJ>#R-KS\4:,.8b:M*,NQ@4_$ 0"B(8_3X4GN !G"c:"B]^Q:2@>>aB)C]Q .aD%M\>NF4TDX B@2=/EF$SQSIOG$PJIKD6V9PT% BF+%T@O[$N #HY&)Ua&>O  q un~L_%>cyNN(l'$qDp_NMeq}"leOGiYy@w Omg8ORb$\ 2Yf"gCld1Z;Q`e#44|{1 ?b*&CEH5}1  u}t'd$_k^ suUR>' |hfoo*h)vz@DzzY [  )nt ng 'G? ;%zf Hh  H HzfTLJ ^z\uUumK|sWUZK@\NJKGL7:CA0+(>#<+#' ) #          L"!ek_:ZYpdJ B%[CpGZ ]QDU?rm_ \~a m6/;G34}w^S,>1x <=4xE_a3O=]!OsQb77D08-aaP%N /W\h~fdB[ :tW5I=hDG8~ LU3 E{og_FjLo  )~ul+$Mx{\o7D<B4qnbP6+qUQL6D,I][hca32I460 I/J75Aq'f*^}$F!MW22`/<"9-9;IcFHI0PM;H$l0(&$"&#;4G$%8#+,0"/**)'&3Q2 &1&D8 O' /!3I* =g<U-/& ,&&+ (0(($&3' # "#'/'*  2-". )9+2$%<#'==)8,%%,#. ')- :/X!~',||AACAATCCTATCAGTTTGCGGTGCACAGCAACATACACGTACCTACTATTCCGAATTGAGGAAACCCCAATTACGCAACTTGAAGGAAAGAAGACCAACGGCTCCCCCAAGCGCTATAACGACGTCGCACGCAAAACGGACGCAGCAATCCCACCACGTGGAGGAAAGAGCCAGGGCGTGCCACCCACTTCTAGAATAAATAATTAAGGAATCCCGAAAGTATTACACCACTACTAAAAAAGGTGGAAGTCTGGGCGGCAGGGTTACGGGGCATCCACAGCATGTATTTTCGAGCCGAAGTTCTGTGCATAGCATGGAGACCACCCAAAAGAAABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-06-30 22:24:27 -04:00AEPt?QAEPt?QAPFN U/APXV2APrN 1ZAPrV1APrX}}1cARTNASPFASPtfASPtfB1PteB1PteBCTSj6BufT K/CMNT 1CTIDK CTNMKCTOwK CTTL 1CpEPDATA@DATA@DATA@DATA@DATA  5DATA DATA DATA &DATA ?N~U8DATA ?N~DATA ?N~RpDATA ?N~ DCHTDSamDySN 0Dye#DyeN0DyeN1DyeN1 DyeN1DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0EVNT0EVNT0FWO_GATCGTyp!!0HCFGCEHCFGHCFGHCFG==InScInVtLANEPLAST~~hLIMS!!1)LNTD2LsrPaMCHNMODF1JMODL3730NAVGNLNE`NOISOOfScTP1AMN\P1RLNZSP1WDN_P2AMNdP2BANNg_P2RLNb'PBASNNQ1PBASNNOPCONNNYPCONNNWPDMFUPDMFOPLOCNUPLOCNRPSZE`PTYPK'PXLBRGNmKRMXV1RMdNBRMdV1RMdXBRPrNJRPrV1RUND RUND RUND RUND RUNT $RUNT /RUNT RUNT Rate 0RunN""JS/N%OSCAN@SMED SMLtSMPLTSPACAf*cSPACOSPACAf*cSVER4.0SVER j+SVER,,0]SatdTScal@Scan@TUBEA10Tmpr<UserphARphCHOphDYbigphQLcphTRphTRCThis is the confidence range *ABI_Limits..FVocAEPt?QAEPt?QAPFN U/APXV2APrN 1ZAPrV1APrX}}1cARTNASPFASPtfASPtfB1PteB1PteBCTSj6BufT K/CMNT 1CTIDK CTNMKCTOwK CTTL 1CpEPDATA@DATA@DATA@DATA@DATA  5DATA DATA DATA &DATA ?N~U8DATA ?N~DATA ?N~RpDATA ?N~ DCHTDSamDySN 0Dye#DyeN0DyeN1DyeN1 DyeN1DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0EVNT0EVNT0FTabxFVocxFWO_GATCFeat//xQGTyp!!0HCFGCEHCFGHCFGHCFG==InScInVtLANEPLAST~~hLIMS!!1)LNTD2LsrPaMCHNMODF1JMODL3730NAVGNLNE`NOISOOfScTP1AMN\P1RLNZSP1WDN_P2AMNdP2BANNg_P2RLNb'PBASNNQ1PBASNNOPCONNNYPCONNNWPDMFUPDMFOPLOCNUPLOCNRPSZE`PTYPK'PXLBRGNmKRMXV1RMdNBRMdV1RMdXBRPrNJRPrV1RUND RUND RUND RUND RUNT $RUNT /RUNT RUNT Rate 0RunN""JS/N%OSCAN@SMED SMLtSMPLTSPACAf*cSPACOSPACAf*cSVER4.0SVER j+SVER,,0]SatdTScal@Scan@TUBEA10Tmpr<UserphARphCHOphDYbigphQLcphTRphTRKat)ecH<]O9Y}TU}xE_q-%Qvqfkv4)xJt;["*Kky#!#fn2lsa!ly^pd1v/gg61C`wWsV&&?-}U`aK|`Vc.O_l;5)F}oP5?z#`g59Wvf0.w1WZAPn\G+6'UH[2g[jWcgnW6Obe"`dbB-Y@iGu2tZG+F7u2]Hn~+luKnPv@+Idp"iK][UcydLC2fo;LY&0]g0b) e:=Us1HB+{fU2sA>i@8/(8\_pzN02%B4O7yr[q?Ib\f. u>fIwV1P%O`5IB+5|jw3U{hYI+oHN+a,|&bX*F8Ba+b8EYWC.%oKO> `VsIefaqQl&A6PG>}Oujd+[Y.m/wBN $`?"*J[x5FaGwpBT9\qwH=qd)B*vH%lF1?HOeCvpN^3jNRjnMgx*&`R@){wJUo6X?/WZ#&a:OcP$SXtmx+9Hgn<_swW^$buJrUlM;_}$A>e6bZ( iu2Zv&1`Z=WcDArh]|Ko5qS+SP7T]TcSI*$6ey(B~beaA({]/wQ2a52rW.X}=1m)h>VO@F$^Rc(mR#gJh=V1xMkIiO5+znJO!p8Npd-(bcnP6GZ]#(lCKOMQw T"S2Mmn[Ca,k +9}WQ@UzbaI23ttrRXc|LVn!<|'kU$3g;Z6Obl% eIe5_@{&]t[XS_Q7u2&q6X+9mvH%lFivFUUsDG+TK#6] TRFM/|gEmpt^14t.N^n"AQcG|Tc,[60_ySssW*=x xeinyk%&`4'3S/vUN|~6>udrV=y`6@vulc*{7=hI8J)T(k"T'o0_s2#O|!Y<,h`+m&wE6Gz zF/"@>oW~!zFHyErN3W4KD@w JU6PX `HO5QfOl>o XSudrH>PWYQIxwcL*L@rIwYWgUTjLj.u%xfmq;MmN2CgGz^nLoT.i:"\oE):|j!]3B68l|ap'Ic3XkSnwkI6 +FwMzbU`AtGbI.J:*yEwiOOd&79?Ou+:n%ra$NwBx6Ks;6R&)Y?Z8Z~9}_{J^z(aT20p/(ZA$den?,}& b!a7QAQ>UbeP *"Y3hk?^NHE,v*(]BcYAFc=gMAEPt?QAEPt?QAPFN U/APXV2APrN 1ZAPrV1APrX}}1cARTNASPFASPtfASPtfAUDTdB1PteB1PteBCTSj6BufT K/CMNT 1CTIDK CTNMKCTOwK CTTL 1CpEPDATA@DATA@DATA@DATA@DATA  5DATA DATA DATA &DATA ?N~U8DATA ?N~DATA ?N~RpDATA ?N~ DCHTDSamDySN 0Dye#DyeN0DyeN1DyeN1 DyeN1DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0EVNT0EVNT0FTabxFVocxFWO_GATCFeat//xQGTyp!!0HCFGCEHCFGHCFGHCFG==InScInVtLANEPLAST~~hLIMS!!1)LNTD2LsrPaMCHNMODF1JMODL3730NAVGNLNE`NOISOOfScTP1AMN\P1RLNZSP1WDN_P2AMNdP2BANNg_P2RLNb'PBASNNQ1PBASNNOPCONNNYPCONNNWPDMFUPDMFOPLOCNUPLOCNRPSZE`PTYPK'PXLBRGNmKRMXV1RMdNBRMdV1RMdXBRPrNJRPrV1RUND RUND RUND RUND RUNT $RUNT /RUNT RUNT Rate 0RunN""JS/N%OSCAN@SMED SMLtSMPLTSPACAf*cSPACOSPACAf*cSVER4.0SVER j+SVER,,0]SatdTScal@Scan@TUBEA10Tmpr<UserphARphCHOphDYbigphQLcphTRphTR