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 (''121;:;CCCLLLUUU]]\eedlllttt{{{<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 2c39cc48bf46407d918c58a7347da87fSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-05-21_01-29_04453130Seq16439134104_134104mfagan96-Well#""#""#"#""#"######"#"##"""##"###############################################"######"#"########"######"####################################################"#"###########"##################"######"#####################################"###"#########################""#########"############################################"#########################################""####"#"#########################################"###"###"########"#######################################""######""####################################"######"#"##"##########################"##########"###########"##"######################################"##"###################"#############"############""######""###########################################""####"######""###"###############"###"#########################"#########"#############################"#####"###"#############"############"########"###"#####"#############################################"##########"#############"#"#################################################################"######"##"##""#####""########"####"#########################""#####""######"#############"##"####"############"#""##"###""####################"#########################""##"#""##############################"######""######################K1313_G7_Pavonia_schiedeana_psbA3_fKB_3730_POP7_BDTv3.mobSeqPop7z;:98789=AEIKLKIFCA@AABCCCCDDDB@<7433579:9864457;?CFHIGECAADGMSX\_``_][ZZ[]`a`\VOIEDEFEB=70*$ 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"##"                               dA?}A#Ac(A0KB.bcpKB_3730_POP7_BDTv3.mobtermAGAATCTCTGACTAGCTGCTATTGAGCTCCATCTACAAATGGATAAGACTTTCGTTTTAGTGTATACGAGTTTTTGAAAATAAAGGAGCAATAACCAATTTCTTGTTCTATCAAGAGTGTTGGTATTGCTCCTTTATTTAGATTTAGTAGTCTTTCTTTTTTTTACATTTCTTTACATAAGTTTGTTTTTTCTTTACTTTCACGTTAAGTTTACCTTTTTTATTTTACCCTATTTATATTTATTTCATAAGAAAAAAAAAAAGATTTTTAATGAGTTTGATTTCGTTTTATTGGATTTGACTTAGTATTATACGTTCCTTATGTACTACTATAATATAGTAACGAATTTCTATAAATATAGAAAAAAAGTAAAAATAAAATTCCAATCTCTATCTTTTATTTTTACTCTAAAATCAAAACTTTACAAAAGATTGTAATTTTTTATATATTGTATCTCAGAAATAAGATAAAAAAAGAATGATGAATGATAGAAATTCACATCTTTTAAATACAAATTTCAAAGATTTAAACAAATAAAAACAAAATAGTATAGTGAAAAATACTATACTGAAAAATACTATAGTAATAGTAGCGGGGGCGGATGTAGGCTAGTGGGATCAAGGGCAGTGGATTGTGAATCCACCCATGCCACGAAAGAAGAATCTCTGACTAGCTGCTATTGAGCTCCATCTACAAATGGATAAGACTTTCGTTTTAGTGTATACGAGTTTTTGAAAATAAAGGAGCAATAACCAATTTCTTGTTCTATCAAGAGTGTTGGTATTGCTCCTTTATTTAGATTTAGTAGTCTTTCTTTTTTTTACATTTCTTTACATAAGTTTGTTTTTTCTTTACTTTCACGTTAAGTTTACCTTTTTTATTTTACCCTATTTATATTTATTTCATAAGAAAAAAAAAAAGATTTTTAATGAGTTTGATTTCGTTTTATTGGATTTGACTTAGTATTATACGTTCCTTATGTACTACTATAATATAGTAACGAATTTCTATAAATATAGAAAAAAAGTAAAAATAAAATTCCAATCTCTATCTTTTATTTTTACTCTAAAATCAAAACTTTACAAAAGATTGTAATTTTTTATATATTGTATCTCAGAAATAAGATAAAAAAAGAATGATGAATGATAGAAATTCACATCTTTTAAATACAAATTTCAAAGATTTAAACAAATAAAAACAAAATAGTATAGTGAAAAATACTATACTGAAAAATACTATAGTAATAGTAGCGGGGGCGGATGTAGGCTAGTGGGATCAAGGGCAGTGGATTGTGAATCCACCCATGCCACGAAAGA !&?Vgn| !/=Qakw *2>IWdmy'1<GUbo| -:EOZfq|'1>HT`ju)7BO[hu%2=JVco{!-:GQ\gs~    * 7 D N Y e r     $ 0 = J W d p |  # - 9 D O \ i t     & 2 = I T a m y   - : D P ] i v +6BO[et)4AMWcq| $1>JVbn{ ".:EQ]ju&3?LXdq| #/<HU`lw)6CNXdr'2?JXbp| $0<HTalx %/<HQ_kv .:GP\hu)4?KWco(3=GR\fr}(5BMXer}  -=HR^lx ")4>HScn{ ",5@HWcmv !&?Vgn| !/=Qakw *2>IWdmy'1<GUbo| -:EOZfq|'1>HT`ju)7BO[hu%2=JVco{!-:GQ\gs~    * 7 D N Y e r     $ 0 = J W d p |  # - 9 D O \ i t     & 2 = I T a m y   - : D P ] i v +6BO[et)4AMWcq| $1>JVbn{ ".:EQ]ju&3?LXdq| #/<HU`lw)6CNXdr'2?JXbp| $0<HTalx %/<HQ_kv .:GP\hu)4?KWco(3=GR\fr}(5BMXer}  -=HR^lx ")4>HScn{ ",5@HWcmv )%" .& '3 1:"5::-:::6:$'::::6>>>#&>>/88'>/>>>>>>>+%88>>8>>>11>>>>>>>>>6/>>>>>>>>;>>;;>;;>1&..>38>,>>;>>;;>>>>;>>>;;>;>>;;>>>>>>>>>>>>>>>>>/>8,>>>>>>6>>,>>>>>>>>8>>>>83$&3&%83/*8>8>8>+8>>>>>3>3>>/8888&'88>>+8>>>-3*888>22..*&/888>/888+8)&>&.*2&8'>>888>4&44>2/6!*6&!>*6>4>/446/*44"4&2:6%6'#)+""*%"::4'!" .%"4#4::66:"# !02*"/*  !#!6"!# 4/00&) *26" ))12/(/)%- :'5*&#%#'! # 3 .    ! ##   #  (   ' ('* $' ) '$%"/#  1!%   #$,' & # *%(#"     )%" .& '3 1:"5::-:::6:$'::::6>>>#&>>/88'>/>>>>>>>+%88>>8>>>11>>>>>>>>>6/>>>>>>>>;>>;;>;;>1&..>38>,>>;>>;;>>>>;>>>;;>;>>;;>>>>>>>>>>>>>>>>>/>8,>>>>>>6>>,>>>>>>>>8>>>>83$&3&%83/*8>8>8>+8>>>>>3>3>>/8888&'88>>+8>>>-3*888>22..*&/888>/888+8)&>&.*2&8'>>888>4&44>2/6!*6&!>*6>4>/446/*44"4&2:6%6'#)+""*%"::4'!" .%"4#4::66:"# !02*"/*  !#!6"!# 4/00&) *26" ))12/(/)%- :'5*&#%#'! # 3 .    ! ##   #  (   ' ('* $' ) '$%"/#  1!%   #$,' & # *%(#"    ? ; +,?VJ_=2WCGD+?%;,7" ^OT -4U& %$Q1`LQ4GCED2-;$X1/@E 7I$b25YPWR "C';9F/ 3Z$!-\C"/0!b. JFIKJA6?S,^.PL'']II65)27,,) 1088P+S\ 2G0$KM'J-350,H3';>&\S*U6I =@=2RO4G =/A*K_G@-Y>+G2.)30<O`!JP'5HJT52I LD-GT&>GLRR!'(bIN@AdV6H[6"G& !D;?-F5('\\aBF4:I1N*[*#/ 5Q 3 -c >9+BO#@*O:$,- L68\ UO);3 38XH2GK*32N,B>5RJ8Za GZ?% 7<F9dZF K.0C'G`/HaFKO8 T>(:# M(A2*CY 2FZ WY21V,`0F)!E`7`-#B"G`0<24N)HX [BX#A2#A2#+29A8#-7AK% 58/ 2Q #?[/[ M cL^FU+S7[92BAS 2>5/2 GO*@ D$2@$".$P_<Z2:^22/@$J3W@$2.]DL)5/*82_C2, 5ZE/11lO7xz2f*A?& 366[<pX U{V$I~x/!X: *+g 9>zZ1W1?}=  >g0cH:'L!T >(na4`Gclb6zfe6q5\b2x!z!@H_nO;g ]oq83% KA`emE5>P+%>V Y BS;liiYIbFlZ4m:s'%Ga`Vaf2(SM'%7[r4rx[o8p,.*D^/YpsC~|j)s1]&NbHK(mVLjbm]:8b V S k{l7*Q{ .a^-;//<9cOig W4 \_/uxtQHDV!,$"[;h RDPbE $zr2d+)|3E;]q0NW5v-3Ec5|'J'Kt(7 r s&mR7oxsHN). `ztupa^8v\HZ405?>A=@&:7D5B@@%&$#;>q>6&#$'..)8,);")!  !  *)"(&6#!,% .&# % +** !!.. (  : B  & )/+!.0)%6$03/ 18G7I)>1%C+5;.7D"!"&)?C(8GH&5L[82*AFME/=MF2>^BKA7KT8G$:NUK[pNAGC)374B\FUA8DN`N?>DXF2PPPP]9U5CcKXiD>OBV DCWCI?VBn_lIiDONW [i7^9=XMM[MQRXoMM[YUaYs}dUMr)MMv:n;.lllllllIClllll{ro]eUUllaUmUylUBB)(>_qNsb]n]wooxkmmmmyyyYp 6d/a^p7p)r48p B 2XK03pp{vVg',~&Vp(pXscpp ;xpJp-I~Vw:`pr,pp}pb~ p`pg/8d,RR4ups ppppx_ \35 ^,*g d2p\0-Ab pdpppp.XvONr=quwq'>@un4@0 P'1?t!:t+334#}/)7wFq*J6'ReZC$*]92(#d'W+$>$I))/9t58JIC"F^A>6;78-F$C#>3#**@ +8 !2!R 9'Z:,`C, @E"?JF6#BG%f .6HQu9f?!PJsBz/:41D/FDi_jaHVfVigq~UH@E@$a<zajyB1fKX!F}L%C+^eSB3)c]|xsYVrm^>:xHHQa\ E9o{aNIP`3wk/jLHLOayp0C=yCT6VE>K E<X~ MR$d,2+V\jH Qr5G?3=z;GeEe_ J5|_6\\P>,Y:K'+"~ +WUh:_ QUTn,CT{S@l%J!noZ@!OG!7<}e@gBHwdo-(L<&/2rzH Z>8`)MwZ_3}p1@/B8XDhJ(64fz'u9FiCACTATCTAACAATATGCGATAGTGCGCTACATATACGGGCATATGTCACGCTCCGAATTGTATATACGCAACCCCGGTTATGGTTGCGCTATTATCTGGAACGTGGTATGACGAGCCCCAAAGGGCCGGTACCCCACAGTCACATTGTAAGCCATCCCCGAGATACCACACAATACATGAGCCTAGCCCCTGCCCACCAACGAGGTTAGAGTAACAAAAATAGAATGAGATGGGTATAGATAAATCACTTTTTTTCTGTGGATACAGATTATCAGAACTAAATAGAGGTAGACTAAATGGACGAATAATACACGGTTCATACCCACTACTCGGATACAATCTCGTAGCTCTCCTTATGTTTTTTCTGAGGGGGTATGGTACTACGGGGGATAACAAATAAGGGTAATCTGCCGTGGGGTGCATGCCGGATACTGTTAAGGGATAGATAGTGTATCACAGGGAGGCGACCCCCTCCGGGTTATGGGTGAGAGCCACTTACCTCGAATGTACACGTAACTGGGTTAGGGCGACCTATTTTCACCGTATCGTATAGTGCCCTACACTACAGCGTTTTCCACTATTATTAGAAGCTCCCCCGCACATCTCCTCCTGTTAGAACGATCGCTGACCAGTGCTTCTTCGGACAAGACAAGGCAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-05-21 03:17:36 -04:00AEPt>)AEPt>)APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtVASPtVB1PtUB1PtUBCTSBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?l~TDATA ?l~ӕDATA ?l~RmDATA ?l~EDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FWO_GATCGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANE3LAST~~.LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP%P1AM$j P1RL$dP1WD$o0P2AM$yxP2BA~P2RL$tTPBASRPBASPXPCONbVPCON_PDMFTPDMFP<PLOC$ZPLOC$U|PSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 2RUNT (RUNT 5 RUNT !Rate 0?RunN""JZS/N%PSCAN@SMED SMLtSMPL$$TySPACA]SPACP5SPACA]SVER4.0SVER SVER,,/Scal@Scan@TUBEG7Tmpr<UserphARphCHPSphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt>)AEPt>)APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtVASPtVB1PtUB1PtUBCTSBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?l~TDATA ?l~ӕDATA ?l~RmDATA ?l~EDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTabFVocFWO_GATCFeat//GTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANE3LAST~~.LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP%P1AM$j P1RL$dP1WD$o0P2AM$yxP2BA~P2RL$tTPBASRPBASPXPCONbVPCON_PDMFTPDMFP<PLOC$ZPLOC$U|PSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 2RUNT (RUNT 5 RUNT !Rate 0?RunN""JZS/N%PSCAN@SMED SMLtSMPL$$TySPACA]SPACP5SPACA]SVER4.0SVER SVER,,/Scal@Scan@TUBEG7Tmpr<UserphARphCHPSphDYbigphQLcphTRphTRf\mci2aqN8>o Ueh,9n5g~Ri6(*24;x$}FNVUCs-113xT]o7U"y6^i_y>]y&m"o`^ kS\KQpYp&!gL-GI&7.{Mco6m~2HBIUbe1Cd}FxQZEZ,}4HUXYnuPQhoNePv7b{Wj2oFtXyy3@=?]p&)C^DZ=CHTA,h{|dsYuTF}wK[*oCfo;LY&p5S_3I|o6HvFHZ( g>}WqD@~TlW+b@cT]`Aqh(YI`N#G]90Q2w8b`sVJX9rPuEJe-"$9:>`)DhcL(+gbk1^DR!_TcM#yKA[,5S[l'ny#/D?*Uq$[-o9D4/D?**s$[-o_}4]'Z5P%?p M;8'i2{&[y3( ]'p8NpdUuk} 9`^avedsYK%FyP\[38f6Tx*&`_MS)AEPt>)APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtVASPtVAUDT%B1PtUB1PtUBCTSBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?l~TDATA ?l~ӕDATA ?l~RmDATA ?l~EDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTabFVocFWO_GATCFeat//GTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANE3LAST~~.LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP%P1AM$j P1RL$dP1WD$o0P2AM$yxP2BA~P2RL$tTPBASRPBASPXPCONbVPCON_PDMFTPDMFP<PLOC$ZPLOC$U|PSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 2RUNT (RUNT 5 RUNT !Rate 0?RunN""JZS/N%PSCAN@SMED SMLtSMPL$$TySPACA]SPACP5SPACA]SVER4.0SVER SVER,,/Scal@Scan@TUBEG7Tmpr<UserphARphCHPSphDYbigphQLcphTRphTR