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 (''121;:;CCCLLLUUU]]\eedlllttt{{{<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 19837442af73482e9f8da5575baf215aSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-05-21_01-29_04453130Seq16439134104_134104mfagan96-Well#""#""#"#""#"######"#"##"""##"###############################################"######"#"########"######"####################################################"#"###########"##################"######"#####################################"###"#########################""#########"############################################"#########################################""####"#"#########################################"###"###"########"#######################################""######""####################################"######"#"##"##########################"##########"###########"##"######################################"##"###################"#############"############""######""###########################################""####"######""###"###############"###"#########################"#########"#############################"#####"###"#############"############"########"###"#####"#############################################"##########"#############"#"#################################################################"######"##"##""#####""########"####"#########################""#####""######"#############"##"####"############"#""##"###""####################"#########################""##"#""##############################"######""#####################K1313_D4_Piper_sp_psbA3_fKB_3730_POP7_BDTv3.mobSeqPop7z58<BJRXZYWWY]acdb_[WTSSTVUTQNKIGD@;61//13552-'"#(,0223358;>ACEGHJJJJIJKMOPPMHC=9631/-,+**+++++,.39ALWdr0NhyW4ueXMFA?=;97669<@BCEIP[iz;G eBwy?_lVE7,!l?xeYSQQRXdzDM $SjiS(3Lqp`TLGGMYm'~evb&0$Hzrlgc_[XWW[bn T^429teG4)$#%*3D];I:2jD+      *2540)#!'.367776530+% $*/0/*#   %'%  Cb41yr]*B7 r~H%%3DXnzaK8)    +pU3 :Ta $+142,"   "mS F:   %))$ !obqp [k  .dy E]d`$x\%EnV$H!setQ2 1 z5   !Kg1\N ~~vn X$ NX~/eC/$!2COTOB0 (6AGF@5)  #/9?@:1%    *12.&  Q9I<  %4BJKB3! 3{GR@  "T4i{e*B)@[8|T`N\a*  '.0+ NU;v&    $)*&$*,' (5<<4( $%!  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J V, !,Cj[.Dz}M4;` HAkyL% ;pb7(:i a. 3bd9%/U j8/T~b;   !I||L'     "()' "(*'   4]wH# >jwN9?_[@B`vI& <c|W5 *Lsa>!    :eiB#%Dha@# !  1Tz|X6  >_~nP:4BaZ:&$CdlO3 5\X7" (./*    "8N_ii_P>.!   !$&'(()*+,,-..//0123444455567765322234320,)'%%&&%#   GUp+s)TzgH(Y/ ;fOB*RvO~/IpP:/.7Ic0AF>(QWn[K<,2V)g(fZ dGvmighijkkkjihghjloruy} *Ow!Lv{Is@VD>C6UzvuuvvtpiaZSNHC>85348?FNV^fmtz|{vpic^YTNHCACHPW]`aaa``_]XSMGCA@@?=82,+0Bb4wLRVpY\xJ:~j^_itua4(Yp]SNKIFB=969FdfSaAe,sG4/27;=<950,)+6O}&:G!\^W. #%%$#! &4ALTWUPIDAACGJJG@6,!   3+Ecl=e*  #&''&%%$!%kx.1 ^D5o:  . n~1DY+|F    #/<GMMG=1$     '0783* (3:<8/#.@P\`^VJ>4-+-159<>@BDGIJJIHFECA?;62.+*(&#    X0'rZA   +34,<~O ' K~w*:Hx6 $+133.' K2Bh0\NL-[       DeGz3   "%(*-06=FOX_bb_XPIB=951-)''+3>GNNH<.  -tI_% E $nv9KEhN "iT$?C~w]$ )151(  4}G3t2 %-0-% '*(! '--)  )4981&  ,gJrrL u<##&h3q U 'Z&LQ2l4PWs"w; +^(PX@ M$ F8J<S& $,12-% %1781& '/2/'  #+.+#  &(&  "3XLe[0a, #-33.% )263* #)-147=EP^lz~yvuvxz{zvqkd_ZVRMGB=952/,($!  %)($ *UO( &)'! (O}X4"&&#  ,WeQVpxUBE\~yR1   ##   #*-*# -QzpL/%('" !#!  $&$    -KlfK97Fb`?$   <[xrV9"&A]t~q[A*     <le\j^=& %?]{u\G=AQiu]I91,*(%  6X{cE.  !Et _H8,$    LI#AA]AY)_AKB.bcpKB_3730_POP7_BDTv3.mobtermACATCTCTGACTGGCTGCTGTTGAGCTCCATCTACAAATGGATAATGCTTCCTCCTTATGTTAATGTATAGGAGTTGTTGAAGGAGCAATACCAAAACCTTGAAAAACAAGAAATAGGGTATTGCTCCTCCTAATTTACTAGTGTTTTATTTACATTTAATCGACGCGAGCATCATTTTTTTTTAATCGTTTAGGTATATTGTATTATTACTAGAATACAGGAAACCCATATTTAAGGGGCGGATGTGGCCAAGTGGATCAAGGCAGTGGATTGTGAATCCACCATGCGCGAACATCTCTGACTGGCTGCTGTTGAGCTCCATCTACAAATGGATAATGCTTCCTCCTTATGTTAATGTATAGGAGTTGTTGAAGGAGCAATACCAAAACCTTGAAAAACAAGAAATAGGGTATTGCTCCTCCTAATTTACTAGTGTTTTATTTACATTTAATCGACGCGAGCATCATTTTTTTTTAATCGTTTAGGTATATTGTATTATTACTAGAATACAGGAAACCCATATTTAAGGGGCGGATGTGGCCAAGTGGATCAAGGCAGTGGATTGTGAATCCACCATGCGCGA 1?V]mz "1AQYdqy )1BKVcp{ !,5@N\gs{ *4@IT_jt '2?KW_jr~ !+6@MWdlw #0:COYepz&0;GQ\gq}    ( 2 = H T _ j s ~    ) 3 > I U ` k t  + 4 ? I S _ j s ~    % / ; E O [ e q z  1?V]mz "1AQYdqy )1BKVcp{ !,5@N\gs{ *4@IT_jt '2?KW_jr~ !+6@MWdlw #0:COYepz&0;GQ\gq}    ( 2 = H T _ j s ~    ) 3 > I U ` k t  + 4 ? I S _ j s ~    % / ; E O [ e q z    &$(:# *644" 6'":>>>>/>>6>6%68&&/*68;>$+;;;;;;/>+;;;;;;3;>>>>>>>;;>>>;;;>>>1$;38,11+>6.>>>>>>;>;;>>>;>;;>;,>>>;;;;33+;;>;>;>;>;;;;;>>3;;33>>>>>>>>>838/*8  8/*6/88/8883/'88383*8883+'8/88>6888228'%8'/'/*8&)+8//>4&//44/+..)4#!4)&"'..!!%*!    &$(:# *644" 6'":>>>>/>>6>6%68&&/*68;>$+;;;;;;/>+;;;;;;3;>>>>>>>;;>>>;;;>>>1$;38,11+>6.>>>>>>;>;;>>>;>;;>;,>>>;;;;33+;;>;>;>;>;;;;;>>3;;33>>>>>>>>>838/*8  8/*6/88/8883/'88383*8883+'8/88>6888228'%8'/'/*8&)+8//>4&//44/+..)4#!4)&"'..!!%*! + V D13Y9E;4IIOU^M.B] 6ac&9*"&@b!Z"#?`HK\O+*RG"0=Y*; G& Sc$%&  CPJ3H9D1"-4_I8PQ*Y <L L=0: 9*HC>.]<VcbP/OUU>>Q&QUW\J;L61-(T:/^$8#$OWG8D11,& ,c(NPY74Q$AJH63)<QH.B]B@!H.!7#/ \$cL2<K-< B 6:U;L20TIF<8 aA#,I:[#VUSE"#93=O>R=;Yh<")}7j}yAD1-[={|Gz!$|GY \w"o,L&:qLJp\v=3t>-w_A7)0th,5cBqr|o|%%E (INU^w/'OZS5IVbA=7:r d"RRfx, \2M[,,TR o^W H4 [ktkCve^cYKV^oHADN16ON02,(?#3&^@+ +!2(,4)#+           :  "               ; I Q_[p=<epCVB6p{pVdR|mpSw{ZC %Np< ='gp',>$qpkp]t)pK?]+^^Bx%8n0dOG}S-{;vL~C"P?:e.qa5)EG3QJ$2p2ac4jPy/#2'_:%<h<_66NgF%I[DT:<I0%YYgO<7o6@|/M"V-=B=P\lS3C,,J@kF$7F_iH F:;a0C,a_OS)~urMOl\\+V+E8EZDp?$B>a_maG^C[>.vf,`FM:O/na|{c2FYU]I(yfId?q3uDM&_=s@ CATCTCTAACAGTCTCCGCTGGAGCGCAACATACACTTACATAGTACGCAGTCTTCATATGATTATGTAGACCTGCTGAAGGAATCGTTGCAAGCGGAGAATTCTTGGCTATTTACCCTGTAGCGATTAGGAGGGACCAATCCTGAAATCCAGGTAAATCAGACTTTAGTATAGGCCCCACCACCTATCCCATCCCCCCACAGTAGGGGGAAGCGTACTCCATTTGTAGGTAGAGCTTCCGCATATGCCGAGTCGAATAGGGACTTTGAATAATGACTATACTGCAAAAAAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-05-21 03:17:11 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt2ASPt2B1Pt1B1Pt1BCTSBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA 0<`xTDATA 0<`x+DATA 0<`xDATA 0<`xvDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FWO_GATCGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~zLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS֛P1AM$H6P1RL$HP1WD$H~P2AM$HP2BA$$VP2RL$HPBAS$$PBAS$$PCON$$PCON$$ݦPDMFTPDMFֲPLOC$H^PLOC$HPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 2RUNT (RUNT 5 RUNT !Rate 0?RunN""JZS/N%֓SCAN@SMED SMLtSMPLTySPACMSPAC֫SPACMSVER4.0SVER SVER,,/Scal@Scan@TUBED4Tmpr<UserphARphCHphDYbigphQLcphTRphTR!This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt2ASPt2B1Pt1B1Pt1BCTSBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA 0<`xTDATA 0<`x+DATA 0<`xDATA 0<`xvDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTab^FVocMFWO_GATCFeat//GTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~zLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS֛P1AM$H6P1RL$HP1WD$H~P2AM$HP2BA$$VP2RL$HPBAS$$PBAS$$PCON$$PCON$$ݦPDMFTPDMFֲPLOC$H^PLOC$HPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 2RUNT (RUNT 5 RUNT !Rate 0?RunN""JZS/N%֓SCAN@SMED SMLtSMPLTySPACMSPAC֫SPACMSVER4.0SVER SVER,,/Scal@Scan@TUBED4Tmpr<UserphARphCHphDYbigphQLcphTRphTRs)B23Hjb.L3`|z&mm,_?DB~P^lQbaeX7SoR:+:j,$}<7-? |EXoW3Y~+ctPp%!++(aQQ*G]mysG(B-O*@NzRfu4WyYZ(Wa:nuXBT]qK_^_<~|Rs5AVih5t}"^wuCt(3ksKp/(ZU&benN#;98wXu0oPnBT16Y-e+&54bn%&|CO1h,9rgzE<=#d1a-c_[O~}4}ceGf+Ko[!AETRF[+]18`F7IOoUVn"eHts'MP(D`~<)l47y!R}*D]d0oA{Qk@ /kXSE@a!)DS *n&-HOnO@5}"a'3.5s\T7}{W_z>FfM~V0Nt'?'|URmquV*wMCtR7R>(hZ$~I!|spN$t.D~F45$\j|KA[Lz2fJq3IxdO#rTiEBq$?seFF} #o$K2LF%c-O=!VBwfmk=;A{%iTK(>V&U)>`V|I+!K|-o*.AQ>UW!\`C}B-8'i2{2[P/ j\V'i~8m#ulc*{<=pjHOo&eG1Vc#TE"J%mydmr+8/Yb~;H26A{%?_CjLhhSc>7:I1DD[ho* xv'o>s1HT/(J8dR9JK[Yle9Su3*-CdM]4e):EGa9?QLwUF3TP.&"D|;f[5kr+E{@2Km91F#+,@j#GWbhc"~9R %2W5OHu+wGkSOj!Q4X+*flVinJils>:4L\\W'KFxz2jA&owbBV*d>AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt2ASPt2AUDT qB1Pt1B1Pt1BCTSBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA 0<`xTDATA 0<`x+DATA 0<`xDATA 0<`xvDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTab^FVocMFWO_GATCFeat//GTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~zLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS֛P1AM$H6P1RL$HP1WD$H~P2AM$HP2BA$$VP2RL$HPBAS$$PBAS$$PCON$$PCON$$ݦPDMFTPDMFֲPLOC$H^PLOC$HPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 2RUNT (RUNT 5 RUNT !Rate 0?RunN""JZS/N%֓SCAN@SMED SMLtSMPLTySPACMSPAC֫SPACMSVER4.0SVER SVER,,/Scal@Scan@TUBED4Tmpr<UserphARphCHphDYbigphQLcphTRphTR